Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,104 G→C 7.0% W204S (TGG→TCG) ‡ sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,1040GC 7.0% 503.1 / 23.0 144W204S (TGG→TCG) ‡sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (67/65);  new base C (4/6);  total (72/72)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 144 total aligned reads displayed.

TAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCG  >  NC_000913/2652963‑2653247
                                                                                                                                             |                                                                                                                                               
  aCCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACt                                                                                                                                        >  2:1276635/1‑149 (MQ=255)
  aCCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAca                                                                                                                                        <  3:985018/149‑2 (MQ=255)
   ccGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAcat                                                                                                                                       <  1:1155717/149‑3 (MQ=255)
          attattGTCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGTAGCACTGAATGTTCCGTGGACGGAACTGGTGcgcg                                                                                                                                >  2:1774489/1‑149 (MQ=255)
            tattGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGaa                                                                                                                              <  1:1103503/149‑1 (MQ=255)
             attGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAg                                                                                                                             <  3:1896763/149‑1 (MQ=255)
              ttGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAgg                                                                                                                            >  2:1371197/1‑149 (MQ=255)
               tGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGc                                                                                                                           <  1:1094785/149‑1 (MQ=255)
                 gCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAg                                                                                                                             <  3:597676/145‑1 (MQ=255)
                 gCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGa                                                                                                                         >  3:1636681/1‑149 (MQ=255)
                  cAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGaa                                                                                                                        <  4:743522/149‑1 (MQ=255)
                        aTGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTAGACGGAACTGGTGCGCGAAGGCGAACTaaaa                                                                                                                  <  3:1885704/149‑1 (MQ=255)
                         tGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaaa                                                                                                                 <  1:1774489/149‑1 (MQ=255)
                             aaTACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGAc                                                                                                             <  1:701982/149‑1 (MQ=255)
                                  ggCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATg                                                                                                        <  4:1636681/149‑1 (MQ=255)
                                     gcAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAAc                                                                                                     >  4:207395/1‑149 (MQ=255)
                                       aaaTTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaaa                                                                                                                 >  4:143016/1‑135 (MQ=255)
                                       aaaTTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaaa                                                                                                                 <  3:143016/135‑1 (MQ=255)
                                       aaaTTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaaa                                                                                                                 >  4:1880953/1‑135 (MQ=255)
                                            attGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGc                                                                                              >  2:900808/1‑149 (MQ=255)
                                              tGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGa                                                                                                          >  4:1837569/1‑135 (MQ=255)
                                              tGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGa                                                                                                          <  3:1837569/135‑1 (MQ=255)
                                               gATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGat                                                                                           >  1:579553/1‑149 (MQ=255)
                                                aTGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGaa                                                                                                                                          <  3:122228/101‑1 (MQ=255)
                                                 tGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGatat                                                                                         >  1:1364633/1‑149 (MQ=255)
                                                  gcccgcccGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtt                                                                                        >  4:1245028/1‑149 (MQ=255)
                                                    ccgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGaa                                                                                                                        >  3:233012/1‑115 (MQ=255)
                                                    ccgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGaa                                                                                                                        <  4:233012/115‑1 (MQ=255)
                                                    ccgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAcattca                                                                                                                                    >  4:1353680/1‑98 (MQ=255)
                                                    ccgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAcattca                                                                                                                                    <  3:1353680/103‑6 (MQ=255)
                                                     cgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt                                                                                     <  1:900808/149‑1 (MQ=255)
                                                     cgcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt                                                                                     <  3:1032881/149‑1 (MQ=255)
                                                      gcccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    <  1:1317764/149‑1 (MQ=255)
                                                       cccgGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg                                                                                   <  2:579553/149‑1 (MQ=255)
                                                            cTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTcgc                                                                              <  1:1371197/149‑1 (MQ=255)
                                                               cACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    >  4:1566662/1‑140 (MQ=255)
                                                               cACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    <  3:1566662/140‑1 (MQ=255)
                                                                    tttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGAcc                                                                                                            <  2:1112166/111‑1 (MQ=255)
                                                                    tttAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAg                                                                      >  3:1426110/1‑149 (MQ=255)
                                                                       aaCGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTaaaaa                                                                                                                 >  1:633159/1‑103 (MQ=255)
                                                                          gCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  4:930832/1‑149 (MQ=255)
                                                                          gCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  1:25059/1‑149 (MQ=255)
                                                                          gCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  1:1656437/1‑149 (MQ=255)
                                                                          gCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  4:293168/1‑149 (MQ=255)
                                                                          gCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  4:369235/1‑149 (MQ=255)
                                                                            aGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    >  1:981854/1‑127 (MQ=255)
                                                                            aGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    <  2:981854/127‑1 (MQ=255)
                                                                                gTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGa                                                                >  1:969156/1‑143 (MQ=255)
                                                                                   gatgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCTGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAAt                                                       <  3:1220524/149‑1 (MQ=255)
                                                                                   gatgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAAt                                                       >  1:688932/1‑149 (MQ=255)
                                                                                    atgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTgg                                                                                  >  2:17313/1‑121 (MQ=255)
                                                                                    atgaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTgg                                                                                  <  1:17313/121‑1 (MQ=255)
                                                                                      gaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGa                                                                                            <  3:240904/109‑1 (MQ=255)
                                                                                      gaACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTCCACGGAAcatt                                                                                                                                      >  2:1680088/1‑64 (MQ=38)
                                                                                       aaCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACCAACCAATTATc                                                   <  3:1245028/149‑1 (MQ=255)
                                                                                       aaCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATc                                                   <  2:1844156/149‑1 (MQ=255)
                                                                                        aCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg                                                                                   >  4:1684521/1‑116 (MQ=255)
                                                                                        aCCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg                                                                                   <  3:1684521/116‑1 (MQ=255)
                                                                                         ccTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATc                                                   >  4:560415/1‑147 (MQ=255)
                                                                                             gCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGc                                             >  1:1394454/1‑149 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGa                                                                                                              >  4:608824/1‑82 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGa                                                                                                              <  3:608824/82‑1 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt                                                                                     >  2:758514/1‑107 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt                                                                                     <  4:756951/107‑1 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAttttt                                                                                     >  3:756951/1‑107 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACaaa                                                            >  1:1697538/1‑132 (MQ=255)
                                                                                               ccAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCaa                                                        >  2:1367637/1‑136 (MQ=255)
                                                                                                   gTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGc                                       >  3:1432870/1‑149 (MQ=255)
                                                                                                     ttACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAAt                                                       >  2:235051/1‑131 (MQ=255)
                                                                                                     ttACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAAt                                                       <  1:235051/131‑1 (MQ=255)
                                                                                                      tACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGctct                                    <  4:1432870/149‑1 (MQ=255)
                                                                                                              ggACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAAc                            >  3:830630/1‑149 (MQ=255)
                                                                                                               gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    >  3:513913/1‑92 (MQ=255)
                                                                                                               gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATAtttttt                                                                                    <  4:513913/92‑1 (MQ=255)
                                                                                                               gACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACg                           >  3:1878147/1‑149 (MQ=255)
                                                                                                                    atTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGgt                                 >  1:753206/1‑138 (MQ=255)
                                                                                                                    atTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGgt                                 <  2:753206/138‑1 (MQ=255)
                                                                                                                    atTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGc                      >  1:796702/1‑149 (MQ=255)
                                                                                                                        ccGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTa                                                     <  2:90260/114‑1 (MQ=255)
                                                                                                                        ccGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTg                  <  2:796702/149‑1 (MQ=255)
                                                                                                                         cGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCg                                                  >  4:106969/1‑116 (MQ=255)
                                                                                                                         cGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTgg                 <  3:930832/149‑1 (MQ=255)
                                                                                                                         cGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTgg                 <  3:369235/149‑1 (MQ=255)
                                                                                                                             gCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGtttt             <  4:830630/149‑1 (MQ=255)
                                                                                                                               aCTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGt           <  4:633668/149‑1 (MQ=255)
                                                                                                                                cTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGtt          >  3:1533169/1‑149 (MQ=255)
                                                                                                                                cTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGAGAACGAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGtt          <  4:1230791/149‑1 (MQ=255)
                                                                                                                                 tGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg                                                                                   <  4:562440/75‑1 (MQ=255)
                                                                                                                                 tGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTg                                                                                   >  3:562440/1‑75 (MQ=255)
                                                                                                                                 tGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGgt                                 <  3:1612900/125‑1 (MQ=255)
                                                                                                                                 tGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGgt                                 >  4:1612900/1‑125 (MQ=255)
                                                                                                                                 tGAATGTTCCGCGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTa         >  2:1410691/1‑149 (MQ=255)
                                                                                                                                  gAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAg        <  4:1533169/149‑1 (MQ=255)
                                                                                                                                    cagTTCCGTCCACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGt           >  1:1843725/3‑144 (MQ=255)
                                                                                                                                    aTGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCa      >  2:294481/1‑149 (MQ=255)
                                                                                                                                       ttCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCTGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTc   >  1:1010460/1‑149 (MQ=255)
                                                                                                                                       ttCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGAc                                                               >  1:1118135/1‑89 (MQ=255)
                                                                                                                                       ttCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGAc                                                               <  2:1118135/89‑1 (MQ=255)
                                                                                                                                       ttCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTcg  >  3:672277/1‑149 (MQ=255)
                                                                                                                                            tGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTc                                                >  2:1751582/1‑99 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TAACCGATGTATTATTGGCAAGCCATGAAAATACGGCGCAAATTATTGATGCCCGCCCGGCTGCACGTTTTAACGCAGAAGTTGATGAACCTCGCCCAGGTTTACGTCGCGGACATATTCCCGGAGCACTGAATGTTCCGTGGACGGAACTGGTGCGCGAAGGCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCG  >  NC_000913/2652963‑2653247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: