Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,784 C→A 9.3% intergenic (+16/+32) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7840CA 9.3% 186.7 / 9.1 54intergenic (+16/+32)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base C (21/28);  new base A (2/3);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

ACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATC  >  NC_000913/2673641‑2673927
                                                                                                                                               |                                                                                                                                               
aCTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTGTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                            <  2:196251/149‑1 (MQ=255)
aCTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                            <  3:1232859/149‑1 (MQ=255)
    ggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        <  4:1712923/149‑1 (MQ=255)
    ggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        <  2:95760/149‑1 (MQ=255)
    ggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        <  1:568169/149‑1 (MQ=255)
      atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGc                                                                                                                                      <  4:1050111/149‑1 (MQ=255)
             cGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                               <  1:1127061/149‑1 (MQ=255)
             cGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                               <  1:896407/149‑1 (MQ=255)
             cGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGCATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                               <  3:1563125/149‑1 (MQ=255)
                            gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAtt                                                                                                                >  3:75317/1‑149 (MQ=255)
                            gCCAAATATGATTAATTTCTGGAAGAATATCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        >  1:123823/1‑125 (MQ=255)
                            gCCAAATATGATTAATTTCTGGAAGAATATCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        <  2:123823/125‑1 (MQ=255)
                                      aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActc                                                                                                      >  4:1388387/1‑149 (MQ=255)
                                           tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActc                                                                                                      <  4:1468299/144‑1 (MQ=255)
                                           tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActc                                                                                                      >  3:1468299/1‑144 (MQ=255)
                                            ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                          >  2:1229174/1‑123 (MQ=38)
                                            ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                          <  1:1229174/123‑1 (MQ=38)
                                                ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcgg                                                                                                                                               <  4:1002466/98‑1 (MQ=255)
                                                ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcgg                                                                                                                                               >  3:1002466/1‑98 (MQ=255)
                                                ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                                                                                 >  1:57629/1‑128 (MQ=255)
                                                ggAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                                                                                 <  2:57629/128‑1 (MQ=255)
                                                 gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                                                     <  1:1623886/107‑1 (MQ=255)
                                                 gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                                                     >  2:1623886/1‑107 (MQ=255)
                                                             tATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                                               >  3:724881/1‑149 (MQ=255)
                                                                 gCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgt                                                                           <  4:724881/149‑1 (MQ=255)
                                                                  cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                          <  3:1388387/149‑1 (MQ=255)
                                                                   tGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGTCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGttgttg                                                                         >  1:1214074/1‑149 (MQ=255)
                                                                             ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                                                                         <  2:1880149/107‑1 (MQ=255)
                                                                             ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                                                                         >  3:1183628/1‑107 (MQ=255)
                                                                             ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                                                                         >  1:1880149/1‑107 (MQ=255)
                                                                             ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                                                                         <  4:1183628/107‑1 (MQ=255)
                                                                             ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTgg                                                               >  1:1881619/1‑149 (MQ=255)
                                                                                   ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        >  4:1535117/1‑70 (MQ=255)
                                                                                   ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAAc                                                                                                                                        <  3:1535117/70‑1 (MQ=255)
                                                                                      gCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                          <  2:918435/129‑1 (MQ=255)
                                                                                      gCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                                                          >  1:918435/1‑129 (MQ=255)
                                                                                        aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                    >  4:612442/1‑149 (MQ=255)
                                                                                           tACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATga                                                 <  3:612442/149‑1 (MQ=255)
                                                                                              cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                           >  1:1854632/1‑88 (MQ=255)
                                                                                              cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                           <  2:1854632/88‑1 (MQ=255)
                                                                                               gggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                        >  4:1545899/1‑74 (MQ=255)
                                                                                               gggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                        <  3:1545899/74‑1 (MQ=255)
                                                                                                         cAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACa                                                                                                             <  3:1589350/75‑1 (MQ=255)
                                                                                                         cAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACa                                                                                                             >  4:1589350/1‑75 (MQ=255)
                                                                                                             ttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCa                                            >  2:218686/1‑136 (MQ=255)
                                                                                                             ttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCa                                            <  1:218686/136‑1 (MQ=255)
                                                                                                                  tcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatga                          <  2:1214074/149‑1 (MQ=255)
                                                                                                                     aTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                       <  2:1881619/149‑1 (MQ=255)
                                                                                                                                     aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                       >  4:1366604/1‑85 (MQ=255)
                                                                                                                                     aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAt                                                                       <  3:1366604/85‑1 (MQ=255)
                                                                                                                                      tGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCt      >  2:1473393/1‑149 (MQ=255)
                                                                                                                                      tGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                       >  2:1087905/1‑132 (MQ=38)
                                                                                                                                      tGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                       <  1:1087905/132‑1 (MQ=38)
                                                                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc  <  1:1473393/149‑1 (MQ=255)
                                                                                                                                            aTGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                       >  4:403420/1‑126 (MQ=255)
                                                                                                                                            aTGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                       <  3:403420/126‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
ACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATC  >  NC_000913/2673641‑2673927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑