Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 288,399 A→C 5.1% *537Y (TAA→TAC yagH → CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913288,3990AC 5.1% 283.2 / 2.5 78*537Y (TAA→TACyagHCP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (37/37);  new base C (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCA  >  NC_000913/288251‑288547
                                                                                                                                                    |                                                                                                                                                    
cGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTaa                                                                                                                                                      <  1:550035/149‑1 (MQ=255)
cGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTaa                                                                                                                                                      <  2:88742/149‑1 (MQ=255)
 ggTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAAc                                                                                                                                                     <  3:1124369/149‑1 (MQ=255)
    gACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGc                                                                                                                                                  <  4:992890/149‑1 (MQ=255)
       gTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCa                                                                                                                                               <  2:1420267/149‑1 (MQ=255)
             ggCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCgg                                                                                                                                         >  3:213646/1‑149 (MQ=255)
             ggCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCgg                                                                                                                                         <  2:1073846/149‑1 (MQ=255)
              gCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCggg                                                                                                                                        >  2:1749145/1‑149 (MQ=255)
                     aGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTAACCTACGAGCCGGTCTAACGGCTCAGGCCgg                                                                                                                                         <  1:180492/141‑1 (MQ=255)
                     aGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTAACCTACGAGCCGGTCTAACGGCTCAGGCCgg                                                                                                                                         >  2:180492/1‑141 (MQ=255)
                      gCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAg                                                                                                                                <  4:213646/149‑1 (MQ=255)
                         gTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAgcgc                                                                                                                             <  4:966653/149‑1 (MQ=255)
                             gacgacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTaa                                                                                                                                                      >  3:215959/1‑120 (MQ=255)
                             gacgacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTaa                                                                                                                                                      <  4:215959/120‑1 (MQ=255)
                               cgacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCga                                                                                                                       <  3:325661/149‑1 (MQ=255)
                                gacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGTCGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCgag                                                                                                                      >  2:561597/1‑149 (MQ=255)
                                gacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGTTAGACCGGCTcgta                                                                                                                                             <  2:450769/126‑4 (MQ=255)
                                gacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTa                                                                                                                                      >  4:29512/1‑133 (MQ=255)
                                gacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTa                                                                                                                                      <  3:29512/133‑1 (MQ=255)
                                gacTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCAACTACGAGCCGTTAGACCGGCTcgta                                                                                                                                             >  1:450769/1‑123 (MQ=255)
                                 acTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCgaga                                                                                                                     <  3:531990/149‑1 (MQ=255)
                                      aTCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGcgcg                                                                                                                >  1:1542152/1‑149 (MQ=255)
                                       tCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGa                                                                                                               <  1:1749145/149‑1 (MQ=255)
                                          gcgggcgCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTCCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGcc                                                                                                            >  1:1582049/1‑149 (MQ=255)
                                           cgggcgCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGTCGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGccc                                                                                                           <  1:561597/149‑1 (MQ=255)
                                              gcgcGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGc                                                                                                        >  2:372647/1‑149 (MQ=255)
                                                gcgGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCggg                                                                                                                                        <  4:207229/115‑1 (MQ=255)
                                                gcgGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCggg                                                                                                                                        >  3:207229/1‑115 (MQ=255)
                                                        ttcACCGGCGTGTTTGTGGGCCTGCACTGCGAGGACATGAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAgc                                                                                              >  2:1447418/1‑149 (MQ=255)
                                                          cACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGTTAGACCGGCTcgta                                                                                                                                             >  3:813878/1‑97 (MQ=255)
                                                          cACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGTTAGACCGGCTcgta                                                                                                                                             <  4:813878/100‑4 (MQ=255)
                                                            ccGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAgctgct                                                                                          <  2:1542152/149‑1 (MQ=255)
                                                                        tgGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCga                                                                                                                       <  1:12020/108‑1 (MQ=255)
                                                                        tgGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCga                                                                                                                       >  2:12020/1‑108 (MQ=255)
                                                                         gggCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGc                                                                             >  4:706864/1‑149 (MQ=255)
                                                                         gggCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGc                                                                             >  2:450491/1‑149 (MQ=255)
                                                                          ggCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGc                                                                                                             >  2:1246305/1‑116 (MQ=255)
                                                                          ggCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGc                                                                                                             <  1:1246305/116‑1 (MQ=255)
                                                                           gCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCa                                                                           >  4:120390/1‑149 (MQ=255)
                                                                            ccTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGcgcgcgcg                                                                                                                        <  1:1244220/103‑1 (MQ=255)
                                                                            ccTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGcgcgcgcg                                                                                                                        >  2:1244220/1‑103 (MQ=255)
                                                                            ccTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCg                                                                                                          >  2:1867630/1‑117 (MQ=255)
                                                                            ccTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCg                                                                                                          <  1:1867630/117‑1 (MQ=255)
                                                                            ccTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAg                                                                          <  1:372647/149‑1 (MQ=255)
                                                                                      gAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGa                                                                                                               >  4:113167/1‑102 (MQ=255)
                                                                                      gAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGa                                                                                                               <  3:113167/102‑1 (MQ=255)
                                                                                               aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       <  3:706864/149‑1 (MQ=255)
                                                                                               aGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       <  1:450491/149‑1 (MQ=255)
                                                                                                 cggcgACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGa                                                     <  3:120390/149‑1 (MQ=255)
                                                                                                     gACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTc                                                 >  2:1375202/1‑149 (MQ=255)
                                                                                                       cGGCTGCTACGCGGACTTCGACTACTCCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAg                                               <  2:1582049/149‑1 (MQ=255)
                                                                                                           tgctACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTc                                           >  3:205216/1‑149 (MQ=255)
                                                                                                                 gcgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGt                                                  >  4:9863/1‑136 (MQ=255)
                                                                                                                 gcgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGt                                                  <  3:9863/136‑1 (MQ=255)
                                                                                                                 gcgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGAc                                        >  2:1725820/1‑146 (MQ=255)
                                                                                                                 gcgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGAc                                        <  1:1725820/146‑1 (MQ=255)
                                                                                                                 gcgGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAgg                                     >  2:558545/1‑149 (MQ=255)
                                                                                                                       ttCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAg                                                    >  4:777390/1‑128 (MQ=255)
                                                                                                                       ttCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAg                                                    <  3:777390/128‑1 (MQ=255)
                                                                                                                       ttCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAgg                               >  4:403798/1‑149 (MQ=255)
                                                                                                                       ttCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAgg                               >  4:1688971/1‑149 (MQ=255)
                                                                                                                         cGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       >  3:632103/1‑123 (MQ=255)
                                                                                                                         cGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       <  4:632103/123‑1 (MQ=255)
                                                                                                                            ctactTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGggcggc                          >  3:1002835/1‑149 (MQ=255)
                                                                                                                              actTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGa                        <  1:558545/149‑1 (MQ=255)
                                                                                                                               ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGAt                       >  2:403797/1‑149 (MQ=255)
                                                                                                                                ttCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAc                                                           >  4:298593/1‑112 (MQ=255)
                                                                                                                                ttCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCAc                                                           <  3:298593/112‑1 (MQ=255)
                                                                                                                                ttCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAg                                                    <  3:239379/119‑1 (MQ=255)
                                                                                                                                ttCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAg                                                    >  4:239379/1‑119 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       <  1:1576160/111‑1 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCgg                                                       >  2:1576160/1‑111 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGa                                                     >  2:1411637/1‑113 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGa                        <  4:1387202/142‑1 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGa                        >  3:1387202/1‑142 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGAcgcg                 >  4:1100358/1‑149 (MQ=255)
                                                                                                                                     cTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCATCGTCCACGCGGGa                                                     <  1:1411637/113‑1 (MQ=255)
                                                                                                                                      tACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCgg                <  3:403798/149‑1 (MQ=255)
                                                                                                                                        cGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGggcg                            <  4:402813/135‑1 (MQ=255)
                                                                                                                                        cGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGggcg                            >  3:402813/1‑135 (MQ=255)
                                                                                                                                               gTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAg                                >  1:1742339/1‑124 (MQ=255)
                                                                                                                                               gTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAg                                <  2:1742339/124‑1 (MQ=255)
                                                                                                                                                   aaCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTg        <  1:81846/144‑1 (MQ=255)
                                                                                                                                                   aaCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTg        >  2:81846/1‑144 (MQ=255)
                                                                                                                                                   aaCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTcc   <  1:403797/149‑1 (MQ=255)
                                                                                                                                                    aCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCca  >  1:156354/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGTGACGTATGAGGCGTGGAAGCTGTCGGACGACTACATCGGCGGGCGCGGCTTCTTCACCGGCGCGTTTGTGGGCCTGCACTGCGAGGACATCAGCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCA  >  NC_000913/288251‑288547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: