| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,798,703 | G→C | 6.5% | intergenic (‑208/‑461) | stpA ← / → alaE | DNA binding protein, nucleoid‑associated/alanine exporter, alanine‑inducible, stress‑responsive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,798,703 | 0 | G | C | 6.5% | 211.0 / 3.2 | 61 | intergenic (‑208/‑461) | stpA/alaE | DNA binding protein, nucleoid‑associated/alanine exporter, alanine‑inducible, stress‑responsive |
| Reads supporting (aligned to +/- strand): ref base G (29/28); new base C (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
ACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGA > NC_000913/2798573‑2798844 | acaAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAAt > 1:628909/1‑149 (MQ=255) caAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAAtt < 2:628909/149‑1 (MQ=255) caAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAAtt > 4:1689794/1‑149 (MQ=255) tCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaa > 4:1061251/1‑149 (MQ=255) ccTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa < 3:1689794/149‑1 (MQ=255) gcgAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGaa < 3:1061251/149‑1 (MQ=255) gcgAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGaa > 3:1418919/1‑149 (MQ=255) ttATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACa < 4:1418919/149‑1 (MQ=255) cTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATaaa > 1:64518/1‑149 (MQ=255) aaaCTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGa > 2:1666467/1‑149 (MQ=255) aataatTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAAt > 4:825126/1‑118 (MQ=255) aataatTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAAt < 3:825126/118‑1 (MQ=255) ataatTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAAcatccatc > 2:1748142/1‑149 (MQ=255) aatTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCAc < 2:64518/149‑1 (MQ=255) atTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGCCGctctgtt < 2:952007/70‑4 (MQ=255) atTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGCCGctctgtt > 1:952007/1‑67 (MQ=255) atTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGCCGctctgtt < 2:1044436/70‑4 (MQ=255) atTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGCCGctctgtt > 1:1044436/1‑67 (MQ=255) tttCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCa > 1:791625/1‑116 (MQ=255) tttCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCa < 2:791625/116‑1 (MQ=255) tttCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTg > 4:426795/1‑149 (MQ=255) ttCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTgg > 4:691744/1‑149 (MQ=255) tCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGt < 3:426795/149‑1 (MQ=255) aGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCt < 1:1666467/149‑1 (MQ=255) gATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAata > 3:574094/1‑113 (MQ=255) gATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAata < 4:574094/113‑1 (MQ=255) aTTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATTAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTg < 3:67102/142‑1 (MQ=255) aTTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATTAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTg > 4:67102/1‑142 (MQ=255) ttATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACa > 2:2487/1‑116 (MQ=255) ttATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACa < 1:2487/116‑1 (MQ=255) atatTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTctgct < 1:1748142/149‑1 (MQ=255) ttAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa > 1:1345546/1‑86 (MQ=255) ttAGCCATGTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCCCTAATTCCGCAAGCCTGACAAAATCaaa < 2:1345546/86‑1 (MQ=255) gCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa > 1:1168638/1‑83 (MQ=255) gCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa < 2:1168638/83‑1 (MQ=255) ccATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa > 3:1047046/1‑82 (MQ=255) ccATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCaaa < 4:1047046/82‑1 (MQ=255) ttttGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTAc > 2:192544/1‑149 (MQ=255) tgaGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTc < 1:192544/149‑1 (MQ=255) tgaGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTc > 4:955018/1‑149 (MQ=255) aGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGa < 2:1685073/61‑1 (MQ=255) aGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGa > 1:1685073/1‑61 (MQ=255) aTTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGt > 1:1173980/1‑129 (MQ=255) aTTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGt < 2:1173980/129‑1 (MQ=255) aTTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGt > 3:70277/1‑129 (MQ=255) aTTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGt < 4:70277/129‑1 (MQ=255) gATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGtt < 3:1037230/125‑1 (MQ=255) gATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGtt > 4:1037230/1‑125 (MQ=255) ttattGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAAt < 3:691744/149‑1 (MQ=255) tattGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGaa > 1:40425/1‑93 (MQ=255) tattGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGaa < 2:40425/93‑1 (MQ=255) attGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAAcatcc < 1:428783/99‑1 (MQ=255) attGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAAcatcc > 2:428783/1‑99 (MQ=255) gAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGaa > 3:1195628/1‑78 (MQ=255) gAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGaa < 4:1195628/78‑1 (MQ=255) cAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTctgc < 2:404272/108‑1 (MQ=255) cAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTctgc > 1:404272/1‑108 (MQ=255) cAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGa < 3:955018/149‑1 (MQ=255) cAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGa > 4:910406/1‑149 (MQ=255) agagCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGaaa < 1:1487404/144‑1 (MQ=255) agagCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGaaa > 2:1487404/1‑144 (MQ=255) ggcgCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGaaa > 3:887838/1‑78 (MQ=255) ggcgCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGaaa < 4:887838/78‑1 (MQ=255) gcgcTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATaaaa < 1:1187591/73‑1 (MQ=255) gcgcTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATaaaa > 2:1187591/1‑73 (MQ=255) | ACAAAATTTAATCTATTTACAGTCCTGCGCGAGATTATTTCAGCTGTTATTTTCTGAAACTCTCAGAATAATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGA > NC_000913/2798573‑2798844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |