Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,174 T→A 6.7% intergenic (+20/+44) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,1740TA 6.7% 417.5 / 14.4 117intergenic (+20/+44)emrB/luxSmultidrug efflux system protein/S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (54/55);  new base A (4/4);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 132 total aligned reads displayed.

AGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTC  >  NC_000913/2814027‑2814322
                                                                                                                                                   |                                                                                                                                                    
aGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAtt                                                                                                                                                     <  3:1040627/149‑1 (MQ=255)
aGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAtt                                                                                                                                                     <  3:1871059/149‑1 (MQ=255)
 ggggCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt                                                                                                                                                    <  4:1728041/149‑1 (MQ=255)
 ggggCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt                                                                                                                                                    >  1:868224/1‑149 (MQ=255)
      tGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa                                                                                                                                                 <  4:846387/147‑1 (MQ=255)
      tGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGaa                                                                                                                                                 >  3:846387/1‑147 (MQ=255)
      tGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACt                                                                                                                                               <  4:1870803/149‑1 (MQ=255)
       gATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTg                                                                                                                                              <  2:1509669/149‑1 (MQ=255)
        attattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTgg                                                                                                                                             <  2:1795786/149‑1 (MQ=255)
         ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGc                                                                                                                                            >  4:772561/1‑149 (MQ=255)
               ccGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCtttttt                                                                                                                                      >  3:1474921/1‑149 (MQ=255)
                 gCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa                                                                                                                                    >  1:1807720/1‑149 (MQ=255)
                 gCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCa                                                                                                                                    >  1:723399/1‑149 (MQ=255)
                   cAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaat                                                                                                                                  >  1:1686737/1‑149 (MQ=255)
                    aaTGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAtt                                                                                                                                                     <  3:100706/129‑1 (MQ=255)
                    aaTGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAtt                                                                                                                                                     >  4:100706/1‑129 (MQ=255)
                      tGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaa                                                                                                                               >  2:1633841/1‑149 (MQ=255)
                          aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACt                                                                                                                                               <  3:117910/129‑1 (MQ=255)
                          aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACt                                                                                                                                               >  4:117910/1‑129 (MQ=255)
                          aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTg                                                                                                                                              >  3:148961/1‑130 (MQ=255)
                          aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTg                                                                                                                                              <  4:148961/130‑1 (MQ=255)
                          aTCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgt                                                                                                                           <  1:862331/149‑1 (MQ=255)
                           tcttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgtg                                                                                                                          >  3:273367/1‑149 (MQ=255)
                            cttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGa                                                                                                                         <  2:868224/149‑1 (MQ=255)
                             ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                                                                        >  1:1046282/1‑149 (MQ=255)
                             ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                                                                        <  4:1474921/149‑1 (MQ=255)
                                tGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaat                                                                                                                                  >  1:732405/1‑136 (MQ=255)
                                  gATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                                                                             <  1:1739897/139‑1 (MQ=255)
                                  gATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAg                                                                                                                   <  2:1046282/149‑1 (MQ=255)
                                      tCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa                                                                                                               >  4:338246/1‑149 (MQ=255)
                                      tCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa                                                                                                               <  2:723399/149‑1 (MQ=255)
                                          ccGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGa                                                                                                           <  1:1559761/149‑1 (MQ=255)
                                                     ctctTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                >  1:1619229/1‑149 (MQ=255)
                                                     cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgt                                                                                                   <  3:1548748/146‑1 (MQ=255)
                                                     cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgt                                                                                                   >  4:1548748/1‑146 (MQ=255)
                                                     cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                <  4:273367/149‑1 (MQ=255)
                                                     cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGc                                                                                                                                            <  1:853791/105‑1 (MQ=255)
                                                     cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGc                                                                                                                                            >  2:853791/1‑105 (MQ=255)
                                                          ccTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           <  3:338246/149‑1 (MQ=255)
                                                           ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTGACTGACTAGATGTGCAGTTc                                                                                            >  1:1612832/1‑147 (MQ=255)
                                                           ctgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTCAGCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTc                                                                                            <  2:1612832/147‑1 (MQ=255)
                                                            tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                               >  3:1715974/1‑143 (MQ=255)
                                                            tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                               <  4:1715974/143‑1 (MQ=255)
                                                            tgctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTg                                                                                         <  2:1686737/149‑1 (MQ=255)
                                                                  ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                                                                             >  4:1553904/1‑107 (MQ=255)
                                                                  ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                                                                             >  2:686828/1‑107 (MQ=255)
                                                                  ggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaatt                                                                                                                             <  1:686828/107‑1 (MQ=255)
                                                                   gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATa                                                                                                                    >  1:141690/1‑115 (MQ=255)
                                                                   gCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATa                                                                                                                    <  2:141690/115‑1 (MQ=255)
                                                                        tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt                                                                             <  2:1807720/149‑1 (MQ=255)
                                                                          tgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                           >  2:1596545/1‑149 (MQ=255)
                                                                             tttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATtgt                                                                                                                           <  1:1613129/98‑1 (MQ=255)
                                                                                    aaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAg                                                                 >  1:806153/1‑149 (MQ=255)
                                                                                     aCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           >  1:200204/1‑122 (MQ=255)
                                                                                        gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGGTGTGCAGTTCCTGCaa                                                                                      >  4:1254505/1‑124 (MQ=255)
                                                                                        gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCaa                                                                                      <  3:645025/124‑1 (MQ=255)
                                                                                        gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCaa                                                                                      >  4:645025/1‑124 (MQ=255)
                                                                                        gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCaa                                                                                      >  4:1568361/1‑124 (MQ=255)
                                                                                        gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCaa                                                                                      <  3:1568361/124‑1 (MQ=255)
                                                                                         ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt                                                                                                             >  1:997460/1‑100 (MQ=255)
                                                                                         ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt                                                                                                             <  2:997460/100‑1 (MQ=255)
                                                                                           aaatGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTgg                                                                                                                                             >  1:1432838/4‑66 (MQ=255)
                                                                                           aTTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc                                                                                <  3:1448895/127‑1 (MQ=255)
                                                                                                gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt                                                     <  2:806153/149‑1 (MQ=255)
                                                                                                gcgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt                                                     <  2:554970/149‑1 (MQ=255)
                                                                                                 cgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                >  4:827347/1‑105 (MQ=255)
                                                                                                 cgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa                                                                                                <  3:827347/105‑1 (MQ=255)
                                                                                                  gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           >  1:1122674/1‑109 (MQ=255)
                                                                                                  gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc                                                                                           <  2:1122674/109‑1 (MQ=255)
                                                                                                         gcggcgGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCg                                            >  2:995582/1‑149 (MQ=255)
                                                                                                                      tGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                               >  1:610183/1‑149 (MQ=255)
                                                                                                                       gcgcACTACGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                              <  2:1619229/149‑1 (MQ=255)
                                                                                                                            cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                               >  3:1763797/1‑143 (MQ=255)
                                                                                                                            cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                               <  4:1763797/143‑1 (MQ=255)
                                                                                                                             tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACt                                                                                                             <  2:807049/64‑1 (MQ=255)
                                                                                                                             tAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGc                        >  4:1050754/1‑149 (MQ=255)
                                                                                                                               aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                      <  2:722787/149‑1 (MQ=255)
                                                                                                                                gtgcAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAc                     >  1:579028/4‑149 (MQ=255)
                                                                                                                                gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc                                                                                                              <  2:1238959/60‑1 (MQ=255)
                                                                                                                                  aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcgc                   <  3:1050754/149‑1 (MQ=255)
                                                                                                                                    aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc                                                                                  <  2:1845318/84‑1 (MQ=255)
                                                                                                                                    aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa                                                >  3:57768/1‑118 (MQ=255)
                                                                                                                                    aaCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa                                                <  4:57768/118‑1 (MQ=255)
                                                                                                                                     aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttct                                                             >  3:152282/1‑104 (MQ=255)
                                                                                                                                         aGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGGTAGTTTACTGTCTAGATGTGAAGTTCCAGCAACTTCTCTTTCGTAAGTGCCCGTTCTACGTTCCTGTTGATGCGTACGTCACTTTCCAGAATGCTATGCGAAatat            >  2:1208534/1‑149 (MQ=255)
                                                                                                                                          gCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATc           >  4:1356873/1‑149 (MQ=255)
                                                                                                                                           ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtct                                                                                 >  1:308961/1‑78 (MQ=255)
                                                                                                                                           ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtct                                                                                 <  2:308961/78‑1 (MQ=255)
                                                                                                                                            cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCt         >  1:1147631/1‑149 (MQ=255)
                                                                                                                                            cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCt         >  4:13232/1‑149 (MQ=255)
                                                                                                                                            cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCt         <  3:13232/149‑1 (MQ=255)
                                                                                                                                               ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                              >  4:1034511/1‑125 (MQ=255)
                                                                                                                                               ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                              >  2:1103338/1‑125 (MQ=255)
                                                                                                                                               ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                              <  1:1103338/125‑1 (MQ=255)
                                                                                                                                               ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACgcgc                 <  3:856865/138‑1 (MQ=255)
                                                                                                                                                   tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGaa                           >  4:1477637/1‑124 (MQ=255)
                                                                                                                                                   tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTg        <  4:57338/143‑1 (MQ=255)
                                                                                                                                                   tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTg        >  3:57338/1‑143 (MQ=255)
                                                                                                                                                   tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTc  >  2:51665/1‑149 (MQ=255)
                                                                                                                                                   tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTc  <  1:51665/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTC  >  NC_000913/2814027‑2814322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: