Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,823,838 C→A 7.4% intergenic (‑69/+11) recA ← / ← pncC DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,8380CA 7.4% 825.0 / 39.1 230intergenic (‑69/+11)recA/pncCDNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor/nicotinamide‑nucleotide amidohydrolase; NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (107/106);  new base A (8/9);  total (115/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 230 total aligned reads displayed.

GAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACC  >  NC_000913/2823693‑2823985
                                                                                                                                                 |                                                                                                                                                   
gAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCa                                                                                                                                                  >  3:194089/1‑149 (MQ=255)
        aCCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTAt                                                                                                                                          <  2:1434871/149‑1 (MQ=255)
         ccAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATc                                                                                                                                         <  3:1701863/149‑1 (MQ=255)
         ccAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATc                                                                                                                                         >  1:1516669/1‑149 (MQ=255)
          cAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCGACGATTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCa                                                                                                                                        >  3:419530/1‑149 (MQ=255)
          cAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaa                                                                                                                                        <  4:1050893/149‑3 (MQ=255)
           aaaTTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCaa                                                                                                                                       <  1:1205870/149‑1 (MQ=255)
                  ttCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                <  4:1599052/149‑1 (MQ=255)
                     tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTa                                                                                                                             >  1:176013/1‑149 (MQ=255)
                     tcAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTa                                                                                                                             <  2:889858/149‑1 (MQ=255)
                         tCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGaaa                                                                                                                         <  4:261742/149‑1 (MQ=255)
                             gCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgt                                                                                                                     >  1:824332/1‑149 (MQ=255)
                              cccAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    >  4:14984/1‑149 (MQ=255)
                              cccAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    <  3:677294/149‑1 (MQ=255)
                               ccAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTa                                                                                                                                           <  1:307908/125‑1 (MQ=255)
                                cAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGc                                                                                                                  <  1:37822/149‑1 (MQ=255)
                                cAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGc                                                                                                                  <  2:1155537/149‑1 (MQ=255)
                                      tgcCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAg                                                                                                            <  3:14984/149‑1 (MQ=255)
                                       gccgccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTa                                                                                                                                           >  3:979453/1‑117 (MQ=255)
                                         cgccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTc                                                                                                         >  3:1325624/1‑149 (MQ=255)
                                          gccAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCt                                                                                                        >  4:1348127/1‑149 (MQ=255)
                                            cAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaatt                                                                                                                                      <  1:1008341/117‑5 (MQ=255)
                                              aCGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGt                                                                                                          <  3:1814395/143‑1 (MQ=255)
                                                 cTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGc                                                                                                      <  4:1274597/144‑1 (MQ=255)
                                                 cTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGc                                                                                                 <  4:1372384/149‑1 (MQ=255)
                                                 cTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGc                                                                                                 <  4:1863554/149‑1 (MQ=255)
                                                  tttCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCaa                                                                                                                                       >  4:319906/1‑110 (MQ=255)
                                                        tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGt                                                                                                                                            >  1:341663/1‑99 (MQ=255)
                                                        tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAt                                                                                                                        <  4:1387649/119‑1 (MQ=255)
                                                        tttgtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACCGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCAt                                                                                               <  1:535381/144‑1 (MQ=255)
                                                           gtttTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTa                                                                                       >  1:1870046/1‑149 (MQ=255)
                                                               tcgtcgATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGc                                                                                                 <  2:307973/135‑1 (MQ=255)
                                                                    gATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga                                                                              <  1:1061881/149‑1 (MQ=255)
                                                                     aTAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    >  1:1148627/1‑110 (MQ=255)
                                                                     aTAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    >  3:125745/1‑110 (MQ=255)
                                                                     aTAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacgac                                                                             <  2:1515027/149‑1 (MQ=255)
                                                                     aTAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTAtaa                                                                                                                                        <  3:1122574/90‑3 (MQ=255)
                                                                        gCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                >  1:541704/1‑95 (MQ=255)
                                                                         ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgttg                                                                                                                   <  1:1019443/107‑1 (MQ=255)
                                                                         ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                         <  2:1276763/149‑1 (MQ=255)
                                                                         ccATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                         >  2:1009275/1‑149 (MQ=255)
                                                                           aTTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTa                                                                                       <  3:1521628/133‑1 (MQ=255)
                                                                            tttttACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGtttt                                                                                                                                <  3:896997/91‑1 (MQ=255)
                                                                            tttttACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGc                                                                      >  1:795116/1‑149 (MQ=255)
                                                                                  cTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTa                                                                                                                             >  1:268705/1‑88 (MQ=255)
                                                                                  cTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACg                                                                <  2:1870046/149‑1 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATa                                                                                                                                                <  3:161689/68‑1 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGaaa                                                                                                                         <  1:671539/91‑1 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGaaa                                                                                                                         >  2:671539/1‑91 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    <  3:1190259/96‑1 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    >  2:823424/1‑96 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAttgtt                                                                                                                    >  4:1190259/1‑96 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgac                                                                                >  2:1778975/1‑132 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgac                                                                                <  1:1778975/132‑1 (MQ=255)
                                                                                   tCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACgg                                                               <  3:1452069/149‑1 (MQ=255)
                                                                                   tCCTGTCAGGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATa                                                                                                                                                >  4:161689/1‑68 (MQ=255)
                                                                                     cTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgacga                                                                              <  2:1051388/132‑1 (MQ=255)
                                                                                      tGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCAc                                                                         >  4:1243561/1‑136 (MQ=255)
                                                                                          aTGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcgccgc                                                        >  1:1432990/1‑149 (MQ=255)
                                                                                           tGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCt                                                       >  1:1512540/1‑149 (MQ=255)
                                                                                              cGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGaa                                                    <  3:843223/149‑1 (MQ=255)
                                                                                               gggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAg                                                   <  4:83087/149‑1 (MQ=255)
                                                                                               gggTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAg                                                   >  3:264790/1‑149 (MQ=255)
                                                                                                     tACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTa                                                                                       >  2:284458/1‑107 (MQ=255)
                                                                                                     tACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGAGCATACAGTa                                                                                                                                           <  4:1804927/55‑1 (MQ=38)
                                                                                                      aCCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTccc                                            <  1:621308/149‑1 (MQ=255)
                                                                                                       ccGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCa                                                                  >  4:442134/1‑126 (MQ=255)
                                                                                                         ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAAtt                                                                                                                       <  3:298209/71‑1 (MQ=255)
                                                                                                         ggATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCccgcc                                         <  2:551451/149‑1 (MQ=255)
                                                                                                          gATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTc                                                             <  1:1291260/128‑1 (MQ=255)
                                                                                                              gTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCgg                                       <  4:911437/146‑1 (MQ=255)
                                                                                                               tCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTaa                                   >  2:1103760/1‑149 (MQ=255)
                                                                                                                cAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                  <  2:176013/149‑1 (MQ=255)
                                                                                                                 aaTATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTa                                                                                       <  2:657953/95‑1 (MQ=255)
                                                                                                                 aaTATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATg                                 >  3:1349616/1‑149 (MQ=255)
                                                                                                                  atatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgac                                                                                >  1:918791/1‑101 (MQ=255)
                                                                                                                  atatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTgac                                                                                <  2:918791/101‑1 (MQ=255)
                                                                                                                  atatGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCAt                                                >  3:1820465/1‑133 (MQ=255)
                                                                                                                       ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                  <  4:598241/142‑1 (MQ=255)
                                                                                                                       ttCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCa                           >  4:101958/1‑149 (MQ=255)
                                                                                                                          tGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcg                        >  3:513829/1‑149 (MQ=255)
                                                                                                                           gTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACcgc                       <  3:1375555/149‑1 (MQ=255)
                                                                                                                              gAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCggg                    >  3:1863276/1‑149 (MQ=255)
                                                                                                                                aGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCAtt                                               <  4:1817853/120‑1 (MQ=255)
                                                                                                                                aGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTcc                                             <  2:364889/122‑1 (MQ=255)
                                                                                                                                aGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                  >  2:1714273/1‑133 (MQ=255)
                                                                                                                                aGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCa                  <  3:1034526/149‑1 (MQ=255)
                                                                                                                                 gCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTc                                              <  2:539025/120‑1 (MQ=255)
                                                                                                                                   aaTTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGt                                                              <  4:844863/102‑1 (MQ=255)
                                                                                                                                    ttgataCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                  >  3:546380/4‑129 (MQ=255)
                                                                                                                                    ttgataCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAAt                                  <  4:546380/126‑1 (MQ=255)
                                                                                                                                     ttATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc             >  3:766612/1‑149 (MQ=255)
                                                                                                                                        taCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc             <  4:1763178/146‑1 (MQ=255)
                                                                                                                                        taCTGTATGAGCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc             >  3:1763178/1‑146 (MQ=255)
                                                                                                                                          cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCccgcc                                         >  1:1390620/1‑116 (MQ=255)
                                                                                                                                          cTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCccgcc                                         <  2:1390620/116‑1 (MQ=255)
                                                                                                                                           tGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTcgc                                                           >  4:279419/1‑97 (MQ=255)
                                                                                                                                              aTGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaaa    <  2:1430840/149‑1 (MQ=255)
                                                                                                                                               tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAc   >  3:249285/1‑149 (MQ=255)
                                                                                                                                                gCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAcc  >  4:1526675/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCTGTCATGCCGGGTAATACCGGATAGTCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACC  >  NC_000913/2823693‑2823985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: