Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,917,896 C→A 5.3% intergenic (+83/+149) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,917,8960CA 5.3% 264.1 / 5.3 75intergenic (+83/+149)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base C (31/40);  new base A (2/2);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

GAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAA  >  NC_000913/2917748‑2918009
                                                                                                                                                    |                                                                                                                 
gAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATgc                                                                                                                   <  3:1261176/149‑1 (MQ=255)
 aGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTAATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATgcg                                                                                                                  <  3:159984/149‑1 (MQ=255)
    tgctgGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATgcggcg                                                                                                               <  4:1419183/149‑1 (MQ=255)
     gctgGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGt                                                                                                              <  1:413420/149‑1 (MQ=255)
       tgGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTa                                                                                                            >  2:955529/1‑149 (MQ=255)
       tgGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTa                                                                                                            <  3:810688/149‑1 (MQ=255)
        ggAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATAAGGCGTTaa                                                                                                           >  1:567263/1‑149 (MQ=255)
         gAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAAc                                                                                                          <  1:1404576/149‑1 (MQ=255)
           aCTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAACATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATTACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGc                                                                                                        <  1:1531212/149‑1 (MQ=255)
           aCTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTTTGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGc                                                                                                        <  2:1727141/149‑1 (MQ=255)
           aCTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGc                                                                                                        <  3:445122/149‑1 (MQ=255)
              gTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATgc                                                                                                                   <  4:1434674/135‑1 (MQ=255)
              gTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATgc                                                                                                                   >  3:1434674/1‑135 (MQ=255)
              gTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                                     <  4:1334275/149‑1 (MQ=255)
               ttGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                    <  2:1474996/149‑1 (MQ=255)
                  gACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                 >  3:447830/1‑149 (MQ=255)
                  gACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                 <  4:1298081/149‑1 (MQ=255)
                  gACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                 >  2:611379/1‑149 (MQ=255)
                  gACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATAAGGCGTTAACGccgcatcc                                                                                                 <  2:567263/149‑7 (MQ=255)
                          ggATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                                     >  4:357166/1‑137 (MQ=255)
                          ggATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                                     <  3:357166/137‑1 (MQ=255)
                          ggATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTCGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                         >  4:1511129/1‑149 (MQ=255)
                             tAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                                      <  1:955529/149‑1 (MQ=255)
                                          gAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCa                                                                         >  4:353204/1‑149 (MQ=255)
                                               cAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGaa                                                                    <  1:611379/149‑1 (MQ=255)
                                                  cAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTaaa                                                                             <  4:1470098/137‑1 (MQ=255)
                                                  cAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTaaa                                                                             >  3:1470098/1‑137 (MQ=255)
                                                   aaaaTTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         >  1:684491/1‑108 (MQ=255)
                                                   aaaaTTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         <  2:684491/108‑1 (MQ=255)
                                                     aaTTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTaaaa                                                              <  4:447830/149‑1 (MQ=255)
                                                     aaTTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTaaaa                                                              >  3:1335353/1‑149 (MQ=255)
                                                      aTTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTaaaaa                                                             >  1:1327072/1‑149 (MQ=255)
                                                           cGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAatgat                                                        <  2:1327072/149‑1 (MQ=255)
                                                            gggTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAatg                                                          <  3:688903/146‑1 (MQ=255)
                                                            gggTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAatg                                                          >  4:688903/1‑146 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                           <  1:1048348/111‑1 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                           <  3:747731/111‑1 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                           >  4:747731/1‑111 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                           >  2:1048348/1‑111 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTa                                                     <  3:1511129/149‑1 (MQ=255)
                                                              gTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTa                                                     >  3:1518954/1‑149 (MQ=255)
                                                                 aTGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGAc                                                  <  4:1335353/149‑1 (MQ=255)
                                                                          ttGTCTAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAAt                                                                                 >  1:1341843/1‑109 (MQ=255)
                                                                          ttGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAAt                                                                                 <  2:1341843/109‑1 (MQ=255)
                                                                           tGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        <  3:353204/149‑1 (MQ=255)
                                                                               gAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCaa                                                                        >  3:1861988/1‑113 (MQ=255)
                                                                               gAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCaa                                                                        <  4:1861988/113‑1 (MQ=255)
                                                                                   aGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACAt                                         <  1:1187149/140‑1 (MQ=255)
                                                                                   aGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACAt                                         >  2:1187149/1‑140 (MQ=255)
                                                                                     cAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAgg                              <  4:1518954/149‑1 (MQ=255)
                                                                                            cTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGa                                              >  3:458191/1‑126 (MQ=255)
                                                                                            cTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGa                                              <  4:458191/126‑1 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         <  1:47068/66‑1 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         >  2:1518800/1‑66 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         >  2:47068/1‑66 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACg                                                                                                         <  1:1518800/66‑1 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCaa                                                                        >  1:1759187/1‑99 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCaa                                                                        <  2:1759187/99‑1 (MQ=255)
                                                                                             tttttATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTg                      >  1:85389/1‑149 (MQ=255)
                                                                                                     aCGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        >  4:209221/1‑123 (MQ=255)
                                                                                                     aCGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        <  3:129808/123‑1 (MQ=255)
                                                                                                     aCGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        >  4:129808/1‑123 (MQ=255)
                                                                                                     aCGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        <  3:209221/123‑1 (MQ=255)
                                                                                                                 gATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCaaa  <  2:85389/149‑1 (MQ=255)
                                                                                                                        gACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCacaaca                                >  4:1360702/1‑112 (MQ=255)
                                                                                                                        gACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCacaaca                                <  3:1360702/112‑1 (MQ=255)
                                                                                                                           aaaGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        <  4:1001084/101‑1 (MQ=255)
                                                                                                                           aaaGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATa                                        >  3:1001084/1‑101 (MQ=255)
                                                                                                                           aaaGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGtct      >  2:1334342/1‑135 (MQ=255)
                                                                                                                           aaaGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGtct      <  1:1334342/135‑1 (MQ=255)
                                                                                                                                  cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAGCCAATTAAATTCaa                                                                        <  1:271747/62‑1 (MQ=255)
                                                                                                                                  cTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCaa                                                                        >  2:271747/1‑62 (MQ=255)
                                                                                                                                   tgtgTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGa                                                   <  2:1716513/82‑1 (MQ=255)
                                                                                                                                   tgtgTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGa                                                   >  1:1716513/1‑82 (MQ=255)
                                                                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCa             <  1:859220/116‑1 (MQ=255)
                                                                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCa             >  2:859220/1‑116 (MQ=255)
                                                                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCa             >  4:200953/1‑116 (MQ=255)
                                                                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCa             <  3:200953/116‑1 (MQ=255)
                                                                                                                                            gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCg                    <  4:1866329/104‑1 (MQ=38)
                                                                                                                                            gCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCg                    >  3:1866329/1‑104 (MQ=38)
                                                                                                                                                    |                                                                                                                 
GAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGCCAGCAAAATTCTCGGGTAATGGATGTCTTGTCGAGTAGCAATCGGCTTTTTATGACGCTGGTTTCAGATTGGTGACAAAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAA  >  NC_000913/2917748‑2918009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: