Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,007,466 T→C 10.7% intergenic (+14/‑44) ygeW → / → ygeX putative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,007,4660TC10.7% 361.4 / 27.2 121intergenic (+14/‑44)ygeW/ygeXputative carbamoyltransferase/2,3‑diaminopropionate ammonia lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (53/55);  new base C (6/7);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

Only 100 of 125 total aligned reads displayed.

CGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTT  >  NC_000913/3007321‑3007613
                                                                                                                                                 |                                                                                                                                                   
cGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAg                                                                                                                                                  >  4:923486/1‑149 (MQ=255)
 gTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGc                                                                                                                                                 >  2:157491/1‑149 (MQ=255)
   aTCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTg                                                                                                                                               <  3:1634418/149‑1 (MQ=255)
        tacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCgg                                                                                                                                          <  1:157491/149‑1 (MQ=255)
        tacctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCgg                                                                                                                                          <  1:1420787/149‑1 (MQ=255)
         acctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGc                                                                                                                                         <  3:923486/149‑1 (MQ=255)
           ctacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGccc                                                                                                                                       <  1:1676440/149‑1 (MQ=255)
            tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCct                                                                                                                                      <  4:1876806/149‑1 (MQ=255)
             acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGAt                                                                                                                                                     >  1:442051/1‑133 (MQ=255)
             acAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGAt                                                                                                                                                     <  2:442051/133‑1 (MQ=255)
                 agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAtt                                                                                                                                 >  1:512336/1‑149 (MQ=255)
                 agaagCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAtt                                                                                                                                 >  1:972397/1‑149 (MQ=255)
                     gCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCa                                                                                                                             <  1:146849/149‑1 (MQ=255)
                        aGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAATCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCagag                                                                                                                          >  2:178446/1‑149 (MQ=255)
                           tGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGcc                                                                                                                       <  4:1463282/149‑1 (MQ=255)
                             gAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGTTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAt                                                                                                                                  <  1:1067004/136‑1 (MQ=255)
                             gAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAt                                                                                                                                  >  2:1067004/1‑136 (MQ=255)
                               aGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaaa                                                                                                                   >  4:929565/1‑149 (MQ=255)
                                gCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                                                                                                                  >  4:1723254/1‑149 (MQ=255)
                                 ccTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAttt                                                                                                                                <  2:1249590/134‑1 (MQ=255)
                                  cTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagga                                                                                                                <  3:1723254/149‑1 (MQ=255)
                                  cTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagga                                                                                                                >  2:1106454/1‑149 (MQ=255)
                                   ttATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggat                                                                                                               >  2:14831/1‑149 (MQ=255)
                                   ttATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 >  3:966449/1‑115 (MQ=255)
                                   ttATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 <  4:966449/115‑1 (MQ=255)
                                    tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                              <  3:170055/149‑1 (MQ=255)
                                    tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                              <  2:972397/149‑1 (MQ=255)
                                    tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                              <  4:1277588/149‑1 (MQ=255)
                                    tataTCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggata                                                                                                              <  2:512336/149‑1 (MQ=255)
                                    aggatcatcGCGGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                                                                   <  1:1141369/125‑1 (MQ=255)
                                    aggatcatcGCGGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                                                                   <  1:1741025/125‑1 (MQ=255)
                                    aggatcatcGCGGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTa                                                                                                                                   >  2:1741025/4‑128 (MQ=255)
                                        tcatcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggataggat                                                                                                          >  1:1066089/1‑149 (MQ=255)
                                          atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctc                                                                                                                                     <  2:1770953/120‑1 (MQ=255)
                                          atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctc                                                                                                                                     >  1:1770953/1‑120 (MQ=255)
                                          atcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                                                                        >  4:52160/1‑149 (MQ=255)
                                           tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 >  4:802981/1‑107 (MQ=255)
                                           tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 >  4:145593/1‑107 (MQ=255)
                                           tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 <  3:802981/107‑1 (MQ=255)
                                           tcGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGc                                                                                                                                                 <  3:145593/107‑1 (MQ=255)
                                              ccgcGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaa                                                                                                                     >  4:1231229/1‑132 (MQ=255)
                                              ccgcGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaa                                                                                                                     <  3:1231229/132‑1 (MQ=255)
                                              ccgcGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTcc                                                                                                    >  2:198789/1‑149 (MQ=255)
                                               cgcgATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCg                                                                                                   >  4:1180870/1‑149 (MQ=255)
                                                     gatCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTtct                                                                                             >  2:1089506/1‑149 (MQ=255)
                                                      atCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGACCAGCTGATCGGcccga                                                                                                                                     >  4:1670935/1‑106 (MQ=255)
                                                       tCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTtct                                                                                             >  2:1790634/1‑147 (MQ=255)
                                                       tCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCa                                                                                           <  3:52160/149‑1 (MQ=255)
                                                        ccTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAt                                                                                          <  1:1089506/149‑1 (MQ=255)
                                                         cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGc                                                                                                                        <  4:1815606/118‑1 (MQ=255)
                                                         cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGc                                                                                                                        >  3:1815606/1‑118 (MQ=255)
                                                         cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                                                           >  4:1599308/1‑131 (MQ=255)
                                                         cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                                                           <  3:1599308/131‑1 (MQ=255)
                                                         cTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                         <  3:1180870/149‑1 (MQ=255)
                                                          tGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTg                                                                                        >  4:1148669/1‑149 (MQ=255)
                                                           gTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTg                                                                                                                                               >  1:1406746/1‑93 (MQ=255)
                                                                gTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGAtt                                                                                  <  3:929565/149‑1 (MQ=255)
                                                                   aaTACGCCAAATCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGat                                                                               <  1:178446/149‑1 (MQ=255)
                                                                     tACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGa                                                                                    >  4:1801618/1‑142 (MQ=255)
                                                                     tACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGa                                                                                    <  3:1801618/142‑1 (MQ=255)
                                                                        gCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctc                                                                                                                                     <  2:1832388/90‑1 (MQ=255)
                                                                        gCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctc                                                                                                                                     >  1:1832388/1‑90 (MQ=255)
                                                                         ccAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGcc                                                                         <  3:1148669/149‑1 (MQ=255)
                                                                           aaaCCAGGTTCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                                                                                                                 >  2:1148778/1‑107 (MQ=255)
                                                                           aaaCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                                                                                                                 >  2:446367/1‑107 (MQ=255)
                                                                           aaaCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                                                                                                                 <  1:1148778/107‑1 (MQ=255)
                                                                           aaaCCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                                                                                                                 <  1:446367/107‑1 (MQ=255)
                                                                                 ggTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTtc                                                                                              >  3:1113679/1‑120 (MQ=255)
                                                                                       cTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                                                                        <  2:1529781/104‑1 (MQ=255)
                                                                                       cTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGatat                                                                                                        >  1:1529781/1‑104 (MQ=255)
                                                                                        tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAt                                                                                                                                  >  3:438574/1‑77 (MQ=255)
                                                                                        tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTAt                                                                                                                                  <  4:438574/77‑1 (MQ=255)
                                                                                        tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatag                                                                                                             <  2:1845345/98‑1 (MQ=255)
                                                                                        tGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatag                                                                                                             >  1:1845345/1‑98 (MQ=255)
                                                                                             gAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATaa                                                                    >  3:957214/1‑134 (MQ=255)
                                                                                             gAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATaa                                                                    <  4:957214/134‑1 (MQ=255)
                                                                                                 aaCTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                                                                       <  3:1401218/127‑1 (MQ=255)
                                                                                                 aaCTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGa                                                                       >  4:1401218/1‑127 (MQ=255)
                                                                                                         aaaGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCaa                                                        >  3:1547546/1‑134 (MQ=255)
                                                                                                         aaaGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCaa                                                        <  4:1547546/134‑1 (MQ=255)
                                                                                                           agaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                                                           >  2:1186550/1‑81 (MQ=255)
                                                                                                           agaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagga                                                                                                           <  1:1186550/81‑1 (MQ=255)
                                                                                                            gaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGaa                                                                                      <  4:1610223/101‑1 (MQ=255)
                                                                                                            gaagCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGaa                                                                                      >  3:1610223/1‑101 (MQ=255)
                                                                                                                 gcAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCg                                                                        >  1:1881350/1‑110 (MQ=255)
                                                                                                                 gcAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCg                                                                        <  2:1881350/110‑1 (MQ=255)
                                                                                                                   aaGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                         <  4:641770/91‑1 (MQ=255)
                                                                                                                   aaGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt                                                                                         >  3:641770/1‑91 (MQ=255)
                                                                                                                                 tAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCCGAGGCCAAAAGGTTAGGATATGTCCGTTTTCTCATTGAAGATTGat                                                                               >  2:1511758/1‑87 (MQ=255)
                                                                                                                                 tAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGat                                                                               <  1:1511758/87‑1 (MQ=255)
                                                                                                                                 tAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCaa                                                        >  1:1589637/1‑110 (MQ=255)
                                                                                                                                      ccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACAt                                             <  1:1037394/116‑1 (MQ=255)
                                                                                                                                      ccTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACAt                                             >  2:1037394/1‑116 (MQ=255)
                                                                                                                                         agggCCGACCAGCTGATCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAt                                                                                          <  3:1375517/67‑1 (MQ=38)
                                                                                                                                            gCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGctc                                     <  1:1255121/118‑1 (MQ=255)
                                                                                                                                            gCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGctc                                     >  2:1255121/1‑118 (MQ=255)
                                                                                                                                             ccGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCa     >  1:116729/1‑149 (MQ=255)
                                                                                                                                                aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGctct                                    >  4:1461491/1‑115 (MQ=255)
                                                                                                                                                aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGctct                                    <  3:1461491/115‑1 (MQ=255)
                                                                                                                                                aTCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGctct                                    <  1:1799613/115‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTGAAGATTGATATCGCCGATAACAAATTTTTCAACGGCGAAACATCACCGCTCTTTTCGCAAAGCCAGGCCAAACTGGCGCGCCAGTT  >  NC_000913/3007321‑3007613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: