Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,020,510 G→T 13.3% intergenic (+21/‑30) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,020,5100GT13.3% 291.7 / 29.0 90intergenic (+21/‑30)ssnA/ygfMputative chlorohydrolase/aminohydrolase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (36/42);  new base T (7/5);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACC  >  NC_000913/3020363‑3020656
                                                                                                                                                    |                                                                                                                                                  
caGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGc                                                                                                                                                   >  4:65256/1‑149 (MQ=255)
  gCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                 <  1:1311925/149‑1 (MQ=255)
   cGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                <  3:1015223/149‑1 (MQ=255)
   cGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                <  4:387567/149‑1 (MQ=255)
       aTGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATACCCTCTTCCCTGCGGGAag                                                                                                                                            >  4:141678/1‑149 (MQ=255)
        tGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                                                                                                                           <  1:1326884/149‑1 (MQ=255)
        tGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                                                                                                                           <  4:1132259/149‑1 (MQ=255)
           tCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAaga                                                                                                                                           >  2:744500/1‑146 (MQ=255)
           tCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAaga                                                                                                                                           <  1:744500/146‑1 (MQ=255)
                    tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                          <  4:68396/138‑1 (MQ=255)
                    tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAagag                                                                                                                                          >  3:68396/1‑138 (MQ=255)
                    tgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                               <  3:65256/149‑1 (MQ=255)
                         ttCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAgg                                                                                                                              >  4:798492/2‑145 (MQ=255)
                         gTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaag                                                                                                                          >  1:502736/1‑149 (MQ=255)
                         gTCTATGAAGACCGTCAGTTTAACATCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAgg                                                                                                                              <  3:798492/145‑1 (MQ=255)
                             aTGAAGACCTTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                 >  4:1629885/1‑122 (MQ=255)
                             aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAg                                                                                                                                                 <  3:1629885/122‑1 (MQ=255)
                             aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                                                                                                                      <  3:1826645/149‑1 (MQ=255)
                                    ccGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGa                                                                                                               >  3:664654/1‑149 (MQ=255)
                                      gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                   <  1:1218855/143‑1 (MQ=255)
                                      gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                   >  2:1218855/1‑143 (MQ=255)
                                      gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAc                                                                                                             >  2:1877279/1‑149 (MQ=255)
                                       tCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                >  4:231165/1‑113 (MQ=255)
                                       tCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                                                                                                                                <  3:231165/113‑1 (MQ=255)
                                           tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGCAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                     >  2:931674/1‑136 (MQ=255)
                                           tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                     <  1:931674/136‑1 (MQ=255)
                                           tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt                                                                                                        <  2:502736/149‑1 (MQ=255)
                                                 ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                                                                                                                           <  1:1292247/124‑1 (MQ=255)
                                                 ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                                                                                                                           >  2:1292247/1‑124 (MQ=255)
                                                 ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAgg                                                                                                  >  4:746364/1‑149 (MQ=255)
                                                 ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATATTTCAgg                                                                                                  >  2:1450277/1‑149 (MQ=255)
                                                      ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                     <  3:726520/125‑1 (MQ=255)
                                                      ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                                                                                                                     >  4:726520/1‑125 (MQ=255)
                                                          gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                              >  1:912576/1‑128 (MQ=255)
                                                          gATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa                                                                                                              <  2:912576/128‑1 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                               >  3:234372/1‑109 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAg                                                                                                                               <  4:234372/109‑1 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGgaa                                                                                                                           >  4:629669/1‑113 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                                                                                                                           >  4:1771343/1‑113 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                                                                                                                           <  3:1771343/113‑1 (MQ=255)
                                                            ttCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                                                                                                                           <  3:629669/113‑1 (MQ=255)
                                                             tCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTc                                                                                      <  1:1877279/149‑1 (MQ=255)
                                                                 tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         <  1:472844/94‑1 (MQ=255)
                                                                 tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         <  1:1183090/94‑1 (MQ=255)
                                                                 tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         >  2:1183090/1‑94 (MQ=255)
                                                                 tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         >  2:472844/1‑94 (MQ=255)
                                                                 tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATATTTCAGGCCCGACTCTGTCGAAc                                                                                  <  1:1450277/149‑1 (MQ=255)
                                                                     tGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGgc                                                                              <  4:664654/149‑1 (MQ=255)
                                                                        acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         >  4:1476391/1‑87 (MQ=255)
                                                                        acaAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCTGCGGGAAGAgg                                                                                                                                         <  3:1476391/87‑1 (MQ=255)
                                                                             ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAt                                                                                                          >  2:1633297/1‑113 (MQ=255)
                                                                             ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAt                                                                                                          <  1:1633297/113‑1 (MQ=255)
                                                                             acAGAAAAGCCGCTGCCAGTCTGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCttcagt                                                                      >  4:1683621/2‑144 (MQ=255)
                                                                               aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGa                                                                                                                                              <  2:482818/75‑1 (MQ=255)
                                                                               aGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGa                                                                                                                                              >  1:482818/1‑75 (MQ=255)
                                                                                     gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                   <  2:259371/96‑1 (MQ=255)
                                                                                     gCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga                                                                                                                   >  1:259371/1‑96 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                      >  3:450793/1‑70 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                                                                                                                      <  4:450793/70‑1 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                     >  2:1644966/1‑103 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                     <  1:1644966/103‑1 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                     >  2:1252532/1‑103 (MQ=255)
                                                                                            ccAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTc                                                                                                     <  1:1252532/103‑1 (MQ=255)
                                                                                                   gtgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt                                                                                                        >  4:695044/1‑93 (MQ=255)
                                                                                                   gtgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt                                                                                                        <  3:695044/93‑1 (MQ=255)
                                                                                                           ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                                                                                                                            <  1:819712/65‑1 (MQ=255)
                                                                                                           ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                                                                                                                            >  2:819712/1‑65 (MQ=255)
                                                                                                           ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatgat                                                                                                                  >  1:1691295/1‑75 (MQ=255)
                                                                                                           ggatggatGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatgat                                                                                                                  <  2:1691295/75‑1 (MQ=255)
                                                                                                                gatgCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                   <  3:746364/149‑1 (MQ=255)
                                                                                                                                         cccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGa                                                                   <  4:988738/92‑1 (MQ=255)
                                                                                                                                         cccTCTTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGa                                                                   >  3:988738/1‑92 (MQ=255)
                                                                                                                                           ctctTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                         <  1:721717/132‑1 (MQ=255)
                                                                                                                                           ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgcc                                                             <  4:574574/96‑1 (MQ=255)
                                                                                                                                           ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGcgcc                                                             >  3:574574/1‑96 (MQ=255)
                                                                                                                                            tctTCCCTGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTc                                         <  1:1024492/115‑1 (MQ=255)
                                                                                                                                            tctTCCCGGCGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTc                                         >  2:1024492/1‑115 (MQ=255)
                                                                                                                                                 ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                  >  1:404084/1‑133 (MQ=255)
                                                                                                                                                 ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                  <  2:404084/133‑1 (MQ=255)
                                                                                                                                                 ccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAAcc  >  3:1384198/1‑149 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGcc                                            <  3:904925/106‑1 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGcc                                            >  4:904925/1‑106 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                   <  2:619317/115‑1 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                   <  2:890937/115‑1 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                   >  1:890937/1‑115 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTc                                   >  1:619317/1‑115 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa               >  2:1324555/1‑135 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa               <  1:1324555/135‑1 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa               <  4:902648/135‑1 (MQ=255)
                                                                                                                                                  cGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCaa               >  3:902648/1‑135 (MQ=255)
                                                                                                                                                    gCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                       >  2:1719349/1‑78 (MQ=255)
                                                                                                                                                    gCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                       <  3:1699603/78‑1 (MQ=255)
                                                                                                                                                    gCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                       <  1:1719349/78‑1 (MQ=255)
                                                                                                                                                    gCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGaa                                                                       >  4:1699603/1‑78 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAGCGTGATGGTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACC  >  NC_000913/3020363‑3020656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: