Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,768 A→G 5.7% intergenic (+445/+45) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,7680AG 5.7% 362.7 / 8.8 106intergenic (+445/+45)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (54/45);  new base G (3/3);  total (57/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

Only 100 of 112 total aligned reads displayed.

TTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCAC  >  NC_000913/3183623‑3183916
                                                                                                                                                 |                                                                                                                                                    
ttCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACaa                                                                                                                                                   >  2:1092733/1‑149 (MQ=255)
ttCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACaa                                                                                                                                                   >  2:1305438/1‑149 (MQ=255)
    tatGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTc                                                                                                                                               <  1:1305438/149‑1 (MQ=255)
                    cGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAgg                                                                                                                               <  1:1092733/149‑1 (MQ=255)
                                 tGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAagc                                                                                                                  >  1:1327522/1‑149 (MQ=255)
                                         aTCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGtt                                                                                                                                           <  3:1521873/116‑1 (MQ=255)
                                         aTCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGtt                                                                                                                                           >  4:1521873/1‑116 (MQ=255)
                                         aTCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAAt                                                                                                                       >  2:439326/1‑136 (MQ=255)
                                         aTCATAATGAAAAAGAATGCCTGATATAAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAAt                                                                                                                       <  1:439326/136‑1 (MQ=255)
                                             tAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAg                                                                                                      >  4:213703/1‑149 (MQ=255)
                                              aaTGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAg                                                                                                                            >  2:1087037/1‑126 (MQ=255)
                                              aaTGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAg                                                                                                                            <  1:1087037/126‑1 (MQ=255)
                                              aaTGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGc                                                                                                     >  2:1360359/1‑149 (MQ=255)
                                                            ccTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCt                                                                                                    <  3:1795070/136‑1 (MQ=255)
                                                            ccTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCt                                                                                                    >  4:1795070/1‑136 (MQ=255)
                                                                      aTGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGAc                                                                             >  2:961330/1‑149 (MQ=255)
                                                                       tGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACg                                                                            >  3:1449453/1‑149 (MQ=255)
                                                                        gAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTaa                                                                                                                    <  3:1329236/108‑1 (MQ=255)
                                                                        gAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTaa                                                                                                                    >  4:1329236/1‑108 (MQ=255)
                                                                          aaTTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGAc                                                                             <  2:1536800/145‑1 (MQ=255)
                                                                          aaTTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGAc                                                                             >  3:359022/1‑145 (MQ=255)
                                                                          aaTTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGAc                                                                             <  4:359022/145‑1 (MQ=255)
                                                                           aTTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTAt                                                                        <  3:213703/149‑1 (MQ=255)
                                                                            ttttCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCAtgt                                                                                    >  2:95202/1‑136 (MQ=255)
                                                                            ttttCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCAtgt                                                                                    <  1:95202/136‑1 (MQ=255)
                                                                                   aTAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTc                                                                                                                                               >  1:356576/1‑70 (MQ=255)
                                                                                   aTAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGa                                                                                                                                     <  4:1554421/80‑1 (MQ=255)
                                                                                   aTAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGa                                                                                                                                     >  3:1554421/1‑80 (MQ=255)
                                                                                   aTAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAg                                                                <  4:866910/149‑1 (MQ=255)
                                                                                      gAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAg                                                                <  4:1271177/146‑1 (MQ=255)
                                                                                      gAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAg                                                                >  3:1271177/1‑146 (MQ=255)
                                                                                       aaaGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCt                                                            >  4:1698961/1‑149 (MQ=255)
                                                                                            gaAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAAt                                                       >  2:348344/1‑149 (MQ=255)
                                                                                             aaGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTGCATCTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  2:1612481/1‑149 (MQ=255)
                                                                                             aaGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTGCAGCTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  2:1612482/1‑149 (MQ=255)
                                                                                             aaGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTGCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  4:122087/1‑149 (MQ=255)
                                                                                             aaGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  1:1360359/149‑1 (MQ=255)
                                                                                             aaGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  2:422636/149‑1 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTgg                                                                                                  <  1:435262/103‑1 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTgg                                                                                                  >  2:435262/1‑103 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCaa                                                        >  4:15662/1‑145 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCaa                                                        <  3:15662/145‑1 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  1:783082/147‑1 (MQ=255)
                                                                                               gCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  2:783082/1‑147 (MQ=255)
                                                                                                  gAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCt                                                                                                                                        <  2:468363/62‑1 (MQ=255)
                                                                                                  gAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCt                                                                                                                                        >  1:468363/1‑62 (MQ=255)
                                                                                                   aaTTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCa                                                                                                       <  4:66046/94‑1 (MQ=255)
                                                                                                   aaTTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCa                                                                                                       >  3:66046/1‑94 (MQ=255)
                                                                                                     ttAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGa                                                                              >  3:897159/1‑117 (MQ=255)
                                                                                                     ttAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGt                                                    <  2:1143963/143‑1 (MQ=255)
                                                                                                     ttAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGt                                                    >  1:1143963/1‑143 (MQ=255)
                                                                                                       aaCATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAgcg                                            >  3:1642136/1‑149 (MQ=255)
                                                                                                          aTCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTc                                                          <  1:492372/132‑1 (MQ=255)
                                                                                                          aTCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTc                                                          >  2:492372/1‑132 (MQ=255)
                                                                                                          aTCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCa                                         >  3:636466/1‑149 (MQ=255)
                                                                                                          aTCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCa                                         >  2:391795/1‑149 (MQ=255)
                                                                                                          aTCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCa                                         >  1:951406/1‑149 (MQ=255)
                                                                                                           tCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCt                                                                                                                                        <  1:1251447/53‑1 (MQ=255)
                                                                                                           tCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCt                                                                                                                                        >  2:1251447/1‑53 (MQ=255)
                                                                                                            cTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCata                                       >  1:1592447/1‑149 (MQ=255)
                                                                                                             ttGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCatat                                      >  3:226131/1‑149 (MQ=255)
                                                                                                              tGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGtttt                                                                                                         <  3:1372665/81‑1 (MQ=255)
                                                                                                              tGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGtttt                                                                                                         >  4:1372665/1‑81 (MQ=255)
                                                                                                               gCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACg                                                                            <  3:1248477/109‑1 (MQ=255)
                                                                                                               gCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACg                                                                            >  4:1248477/1‑109 (MQ=255)
                                                                                                                 aTAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGt                                                              <  4:1524038/121‑1 (MQ=255)
                                                                                                                 aTAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGt                                                              >  3:1524038/1‑121 (MQ=255)
                                                                                                                    gCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGttt                               >  2:625504/1‑149 (MQ=255)
                                                                                                                       gggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTAtt                            <  1:625504/149‑1 (MQ=255)
                                                                                                                       gggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTAtt                            >  4:1150620/1‑149 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTCCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCACATTGTGTTTATTg                           <  3:1888742/149‑1 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCa                                                                                                       <  4:515777/73‑1 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCa                                                                                                       >  3:515777/1‑73 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGTTATGCCACCAGGTCTTCAATa                                                      >  4:630783/1‑122 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  4:1580685/1‑122 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  3:1580685/122‑1 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  3:630783/122‑1 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTg                           <  4:636466/149‑1 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTg                           >  4:1888742/1‑149 (MQ=255)
                                                                                                                        ggAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTg                           <  4:1642136/149‑1 (MQ=255)
                                                                                                                            aaaTAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      >  2:174810/1‑118 (MQ=255)
                                                                                                                            aaaTAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATa                                                      <  1:174810/118‑1 (MQ=255)
                                                                                                                            aaaTAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCaa                       >  2:1113334/1‑149 (MQ=255)
                                                                                                                               tAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACt                    >  1:1799849/1‑149 (MQ=255)
                                                                                                                                aaaaaCCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATtgtg                                  >  3:1146896/1‑134 (MQ=255)
                                                                                                                                aaaaaCCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATtgtg                                  <  4:1146896/134‑1 (MQ=255)
                                                                                                                                  aaaCCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCaa                 <  1:391795/149‑1 (MQ=255)
                                                                                                                                    aCCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCAc                                                 <  1:1467580/115‑1 (MQ=255)
                                                                                                                                       gAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATacac            <  3:1150620/149‑1 (MQ=255)
                                                                                                                                        aaGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACAct           >  2:1854026/1‑149 (MQ=255)
                                                                                                                                           ccTGCTGCAACTTCGGTTTCTTTATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACGAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACActccg        <  1:1612481/149‑3 (MQ=255)
                                                                                                                                           ccTGCTGCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTg        <  3:122087/149‑1 (MQ=255)
                                                                                                                                           ccTGCTGCAACTTCGGTTTCTTCATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACTAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTg        <  1:1612482/149‑1 (MQ=255)
                                                                                                                                             tGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGt      <  4:1440453/149‑1 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTa                                                                         >  2:1128066/1‑78 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTa                                                                         <  1:1128066/78‑1 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATacac            >  1:1589301/1‑139 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATacac            <  2:1589301/139‑1 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCAc  >  2:1821159/1‑149 (MQ=255)
                                                                                                                                                 aCAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCAc  >  1:983386/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTCATATGGTCGCATAATAACGGAACAATTATATGATTTTGATCATAATGAAAAAGAATGCCTGATATTAATGAAATTTTCTCATAGAAAGAGAAGCGGAATTAACATCTTGCATAGCAGGGAAAAATAAAAACCGAAGCCTGCTACAACTTCGGTTTCTTGATTAAGGCAGTAAATTAAGCAGCGGTTTTCAGCTGGCTTTACGCTCATGTGCCTGACGGTATGCCACCAGGTCTTCAATAGTCACGAGCGCCATATTGTGTTTATTGGCAAACTCAATACACTCTGGTGCAC  >  NC_000913/3183623‑3183916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: