Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,258,251 T→A 6.9% intergenic (‑241/+34) yhaO ← / ← tdcG putative transporter/L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,258,2510TA 6.9% 199.9 / 5.4 58intergenic (‑241/+34)yhaO/tdcGputative transporter/L‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base A (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

AAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCG  >  NC_000913/3258107‑3258387
                                                                                                                                                |                                                                                                                                        
aaaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTtgt                                                                                                                                      <  3:1430207/149‑1 (MQ=255)
 aaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTtgtg                                                                                                                                     >  2:348858/1‑149 (MQ=255)
      aTGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCAccc                                                                                                                                >  4:1451084/1‑149 (MQ=255)
                     tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCa                                                                                                                                   >  1:1312826/1‑131 (MQ=255)
                     tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCa                                                                                                                                   <  2:1312826/131‑1 (MQ=255)
                        gagaAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCaa                                                                                                                              >  2:281839/1‑133 (MQ=255)
                        gagaAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCaa                                                                                                                              <  1:281839/133‑1 (MQ=255)
                                  tCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTtgtg                                                                                                                                     >  4:1489921/1‑116 (MQ=255)
                                  tCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTtgtg                                                                                                                                     <  3:1489921/116‑1 (MQ=255)
                                        tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAt                                                                                                                                          <  1:1165173/105‑1 (MQ=255)
                                        tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAt                                                                                                                                          >  2:1165173/1‑105 (MQ=255)
                                                 tGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCGCATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTaa                                                                                     <  3:1451084/149‑1 (MQ=255)
                                                          ggAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtc                                                                            >  4:793930/1‑149 (MQ=255)
                                                                 gATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa                                                                               <  4:791437/139‑1 (MQ=255)
                                                                 gATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa                                                                               >  3:791437/1‑139 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa                                                                                                                 >  3:260263/1‑100 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa                                                                                                                 <  4:260263/100‑1 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCa                                                                                                <  2:225305/117‑1 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCa                                                                                                >  1:225305/1‑117 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt                                                                                    <  4:1466475/129‑1 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt                                                                                    >  3:1466475/1‑129 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGAt                                                                 <  4:1272843/148‑1 (MQ=255)
                                                                      gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGAt                                                                 >  3:1272843/1‑148 (MQ=255)
                                                                       aaTTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCaa                                                                                                                              >  1:1759992/1‑86 (MQ=255)
                                                                       aaTTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCaa                                                                                                                              <  2:1759992/86‑1 (MQ=255)
                                                                         ttttCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGcc                                                                                <  1:237428/130‑1 (MQ=255)
                                                                         ttttCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGcc                                                                                >  2:237428/1‑130 (MQ=255)
                                                                              tactaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAAATGTGc                                                                                                                                    >  1:1440338/1‑73 (MQ=255)
                                                                              tactaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACAAATGTGc                                                                                                                                    <  2:1440338/73‑1 (MQ=255)
                                                                               actaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGaaa                                                                                                                      <  2:454727/86‑1 (MQ=255)
                                                                               actaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGaaa                                                                                                                      >  1:454727/1‑86 (MQ=255)
                                                                                 taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGtt                                                              >  3:790402/1‑140 (MQ=255)
                                                                                 taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGtt                                                              <  4:790402/140‑1 (MQ=255)
                                                                                     ttAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTAt                                                 <  3:793930/149‑1 (MQ=255)
                                                                                         tCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGtt                                             <  1:348858/149‑1 (MQ=255)
                                                                                            cAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGAt                                                                 >  1:1318860/1‑126 (MQ=255)
                                                                                            cAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGAt                                                                 <  2:1318860/126‑1 (MQ=255)
                                                                                               gagaATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATc                                       >  1:419287/1‑149 (MQ=255)
                                                                                                      ttcattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGa                                                                                              <  2:835932/87‑1 (MQ=255)
                                                                                                      ttcattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGa                                                                                              >  1:835932/1‑87 (MQ=255)
                                                                                                      ttcattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCg                                <  2:419287/149‑1 (MQ=255)
                                                                                                        cattcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGt                              >  2:1688188/1‑149 (MQ=255)
                                                                                                         attcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATgg                                                                                  <  1:1883063/96‑1 (MQ=255)
                                                                                                         attcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATgg                                                                                  >  2:1883063/1‑96 (MQ=255)
                                                                                                          ttcTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGttt                            >  2:1322504/1‑149 (MQ=255)
                                                                                                             tctATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAATCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCttt                                    <  2:680648/138‑1 (MQ=255)
                                                                                                             tctATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCttt                                    >  1:680648/1‑138 (MQ=255)
                                                                                                               tATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa                                                                               <  3:33919/93‑1 (MQ=255)
                                                                                                               tATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCa                                                                               >  4:33919/1‑93 (MQ=255)
                                                                                                                  ttGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATc                    <  1:1688188/149‑1 (MQ=255)
                                                                                                                          gATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGAt            <  1:1322504/149‑1 (MQ=255)
                                                                                                                           aTCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATc           <  2:319186/149‑1 (MQ=255)
                                                                                                                           aTCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATc           >  1:319186/1‑149 (MQ=255)
                                                                                                                                  aaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCaa                                                                                                           >  1:381738/1‑46 (MQ=255)
                                                                                                                                  aaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCaa                                                                                                           <  2:381738/46‑1 (MQ=255)
                                                                                                                                    aaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCg  >  1:1203297/1‑149 (MQ=255)
                                                                                                                                     ttgggTGCACAAATGTGCACCCAAGGATGTAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCtt                                     <  4:1078955/110‑1 (MQ=255)
                                                                                                                                     ttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCtt                                     >  3:1078955/4‑113 (MQ=255)
                                                                                                                                           gCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATc                                                >  2:1132548/1‑96 (MQ=255)
                                                                                                                                           gCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATc                                                <  1:1132548/96‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
AAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCG  >  NC_000913/3258107‑3258387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑