Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,050 C→A 5.3% G34C (GGC→TGC)  rplN ← 50S ribosomal subunit protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,0500CA 5.3% 935.4 / 25.2 256G34C (GGC→TGC) rplN50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base C (116/126);  new base A (6/8);  total (122/134)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 264 total aligned reads displayed.

GCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447902‑3448198
                                                                                                                                                    |                                                                                                                                                     
  aTTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTg                                                                                                                                                     >  3:718240/1‑149 (MQ=255)
       cATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAg                                                                                                                                                <  4:1121102/149‑1 (MQ=255)
         tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTa                                                                                                                                                 >  2:87483/1‑146 (MQ=255)
         tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTa                                                                                                                                                 <  1:800754/146‑1 (MQ=255)
         tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCg                                                                                                                                             >  1:1643096/1‑149 (MQ=255)
         tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                              <  2:300323/149‑3 (MQ=255)
            aaGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACg                                                                                                                                          >  3:283742/1‑149 (MQ=255)
              gCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGt                                                                                                                                        <  4:1739393/149‑1 (MQ=255)
                  aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:1031082/149‑1 (MQ=255)
                       aGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAc                                                                                                                               >  3:494495/1‑149 (MQ=255)
                                  ggCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTg                                                                                                                    <  1:542920/149‑1 (MQ=255)
                                   gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTAGGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  4:38600/1‑132 (MQ=255)
                                   gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:200377/149‑1 (MQ=255)
                                   gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:458398/149‑1 (MQ=255)
                                    cgacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGAt                                                                                                                  <  4:470480/149‑1 (MQ=255)
                                     gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  3:144710/1‑149 (MQ=255)
                                     gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  1:1138702/1‑149 (MQ=255)
                                      acgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATac                                                                                                                >  3:902068/1‑149 (MQ=255)
                                         aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:1670404/126‑1 (MQ=255)
                                          acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGAc                                                                                                                                           >  1:1830826/1‑118 (MQ=255)
                                          acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        >  3:360307/1‑137 (MQ=255)
                                             cccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAc                                                                                                         >  1:495609/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  3:149524/1‑140 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  3:1843033/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  1:44336/1‑149 (MQ=255)
                                              ccTTCTTGCTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  4:157950/1‑149 (MQ=255)
                                               cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgc                                                                                                       <  3:103900/149‑1 (MQ=255)
                                               cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggcg                                                                                                                                             <  3:1889610/112‑4 (MQ=255)
                                                ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcg                                                                                                                                             >  2:1772423/1‑107 (MQ=255)
                                                  cttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGAc                                                                                                    <  4:891425/149‑1 (MQ=255)
                                                            accacTACCGCCTTCAGCACATCGCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          >  3:1143147/1‑149 (MQ=255)
                                                            accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  4:806450/107‑1 (MQ=255)
                                                            accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  2:401962/149‑1 (MQ=255)
                                                             ccacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAg                                                                                         >  1:697077/1‑149 (MQ=255)
                                                              cacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATacac                                                                                                              <  4:551134/127‑1 (MQ=255)
                                                                   ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   >  1:1071576/1‑117 (MQ=255)
                                                                   ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:1071576/117‑1 (MQ=255)
                                                                   ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCg                                                                                   >  3:1634819/1‑149 (MQ=255)
                                                                     gCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGc                                                                                 >  4:6715/1‑149 (MQ=255)
                                                                       cTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  2:1204994/96‑1 (MQ=255)
                                                                        ttCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTACACCGGAGTTGTCg                                                                                   >  1:734495/1‑144 (MQ=255)
                                                                        ttCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTACACCGGAGTTGTCg                                                                                   <  2:734495/144‑1 (MQ=255)
                                                                          cAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTACGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGt                                                                            >  2:1807173/1‑149 (MQ=255)
                                                                             cacaTCACCTTTTTTGACCTTACCACGCAGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         <  4:283742/149‑1 (MQ=255)
                                                                              acaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAg                                                                        <  3:6715/149‑1 (MQ=255)
                                                                              acaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAg                                                                        >  3:879203/1‑149 (MQ=255)
                                                                                aTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCt                                                                                                                      >  3:845663/1‑101 (MQ=255)
                                                                                 tCACCTTTTTTGACCTTACCACGCGGATTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCAt                                                                     >  1:943267/1‑149 (MQ=255)
                                                                                 tCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGTCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCAt                                                                     >  1:1438743/1‑149 (MQ=255)
                                                                                 tCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          >  2:1645842/1‑128 (MQ=255)
                                                                                  cACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  4:1843033/149‑1 (MQ=255)
                                                                                   aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGAc                                                                              <  1:1563126/138‑1 (MQ=255)
                                                                                   aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAg                                                                   >  1:1782881/1‑149 (MQ=255)
                                                                                      ttttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                              <  2:273643/72‑3 (MQ=255)
                                                                                        ttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGt                                                              <  3:462363/149‑1 (MQ=255)
                                                                                         tttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGtt                                                             <  2:1138702/149‑1 (MQ=255)
                                                                                            gACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          <  2:1799209/149‑1 (MQ=255)
                                                                                             aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  2:1314709/149‑1 (MQ=255)
                                                                                             aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  2:1561105/149‑1 (MQ=255)
                                                                                             aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  4:201507/149‑1 (MQ=255)
                                                                                             aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTACGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  1:1807173/149‑1 (MQ=255)
                                                                                              ccTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTgg                                                        <  4:1688460/149‑1 (MQ=255)
                                                                                              ccTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  4:1154029/148‑1 (MQ=255)
                                                                                              ccTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         >  3:1154029/1‑148 (MQ=255)
                                                                                               cTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  1:1481886/1‑149 (MQ=255)
                                                                                               cTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  3:1404772/1‑149 (MQ=255)
                                                                                                        cgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGAc                                                                              <  4:533411/117‑1 (MQ=255)
                                                                                                           aaTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGc                                           >  1:227553/1‑149 (MQ=255)
                                                                                                             ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTc                                         <  4:350521/149‑1 (MQ=255)
                                                                                                             ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTc                                         <  2:495609/149‑1 (MQ=255)
                                                                                                                cttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      >  2:1572919/1‑149 (MQ=255)
                                                                                                                 ttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    >  3:1546100/1‑118 (MQ=255)
                                                                                                                 ttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  4:1546100/118‑1 (MQ=255)
                                                                                                                 ttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     >  1:1208686/1‑149 (MQ=255)
                                                                                                                    tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      <  4:242813/145‑1 (MQ=255)
                                                                                                                    tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      >  3:242813/1‑145 (MQ=255)
                                                                                                                    tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  >  4:1447939/1‑149 (MQ=255)
                                                                                                                          ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  1:1109654/109‑1 (MQ=255)
                                                                                                                          ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAac                            >  3:538010/1‑149 (MQ=255)
                                                                                                                            tGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               >  4:1264947/1‑128 (MQ=255)
                                                                                                                            tGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  3:1264947/128‑1 (MQ=255)
                                                                                                                             gATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactac                         <  4:442906/149‑1 (MQ=255)
                                                                                                                              aTCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGt                                                                                                                                        <  1:293414/37‑1 (MQ=255)
                                                                                                                                 ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          <  2:575896/112‑1 (MQ=255)
                                                                                                                                 ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          >  1:575896/1‑112 (MQ=255)
                                                                                                                                   gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTga                   <  2:943267/149‑1 (MQ=255)
                                                                                                                                      gatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         >  4:859637/1‑92 (MQ=255)
                                                                                                                                      gatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGAc                >  1:958317/1‑149 (MQ=255)
                                                                                                                                          tCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGa            >  3:554968/1‑149 (MQ=255)
                                                                                                                                           tcgcTACGCAGGCGTAGTCGACGGGGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGa            >  3:1621876/4‑149 (MQ=255)
                                                                                                                                              cTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAAt        >  3:540847/1‑149 (MQ=255)
                                                                                                                                                aCGCCTGCGTAGCGACGGTGCGAGTCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCa      <  2:1438743/149‑1 (MQ=255)
                                                                                                                                                aCGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCa                                                                      <  2:634080/85‑1 (MQ=255)
                                                                                                                                                aCGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCa      <  3:425383/149‑1 (MQ=255)
                                                                                                                                                aCGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCa      <  4:1404772/149‑1 (MQ=255)
                                                                                                                                                  gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCAcc                                                                                             >  4:1781918/1‑60 (MQ=255)
                                                                                                                                                  gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCAcc                                                                                             <  3:1781918/60‑1 (MQ=255)
                                                                                                                                                   ccTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGt   <  4:489291/149‑1 (MQ=255)
                                                                                                                                                    cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGAccc              >  3:1724346/1‑137 (MQ=255)
                                                                                                                                                    cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTc  <  4:1634819/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447902‑3448198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: