Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,051 T→G 5.2% A33A (GCA→GCC rplN ← 50S ribosomal subunit protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,0510TG 5.2% 967.0 / 31.3 263A33A (GCA→GCCrplN50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base T (119/130);  new base G (6/8);  total (125/138)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447904‑3448198
                                                                                                                                                   |                                                                                                                                                    
aTTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTg                                                                                                                                                     <  4:366408/149‑1 (MQ=255)
aTTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTg                                                                                                                                                     >  3:718240/1‑149 (MQ=255)
     cATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAg                                                                                                                                                >  1:335436/1‑149 (MQ=255)
       tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTa                                                                                                                                                 >  2:87483/1‑146 (MQ=255)
       tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTa                                                                                                                                                 <  1:800754/146‑1 (MQ=255)
       tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCg                                                                                                                                             >  1:1643096/1‑149 (MQ=255)
                aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:1031082/149‑1 (MQ=255)
                aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  3:419904/149‑1 (MQ=255)
                     aGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAc                                                                                                                               >  3:494495/1‑149 (MQ=255)
                       aaCCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAccc                                                                                                                             <  1:777717/149‑1 (MQ=255)
                               gggCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     >  2:542920/1‑149 (MQ=255)
                                ggCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTg                                                                                                                    <  4:1749650/149‑1 (MQ=255)
                                 gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:200377/149‑1 (MQ=255)
                                 gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:335436/149‑1 (MQ=255)
                                   gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  3:144710/1‑149 (MQ=255)
                                   gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  1:1138702/1‑149 (MQ=255)
                                    acgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATac                                                                                                                >  3:902068/1‑149 (MQ=255)
                                       aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  2:1670404/1‑126 (MQ=255)
                                       aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:1670404/126‑1 (MQ=255)
                                       aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   >  4:1312500/1‑143 (MQ=255)
                                       aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  3:1312500/143‑1 (MQ=255)
                                        acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACCTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGGCACCCAGAACCTTGATACACAt                                                                                                            >  4:1563047/1‑149 (MQ=255)
                                        acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGAc                                                                                                                                           >  1:1830826/1‑118 (MQ=255)
                                        acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        >  3:360307/1‑137 (MQ=255)
                                           cccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAc                                                                                                         >  1:495609/1‑149 (MQ=255)
                                            ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 <  4:149524/140‑1 (MQ=255)
                                            ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 >  4:215023/1‑140 (MQ=255)
                                            ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  1:44336/1‑149 (MQ=255)
                                             cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgc                                                                                                       <  3:103900/149‑1 (MQ=255)
                                             cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggcg                                                                                                                                             <  3:1889610/112‑4 (MQ=255)
                                              ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgcg                                                                                                      >  2:1460413/1‑149 (MQ=255)
                                              ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgcg                                                                                                      >  4:462363/1‑149 (MQ=255)
                                              ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcg                                                                                                                                             >  2:1772423/1‑107 (MQ=255)
                                               tcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTATTTTGACCTAACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACTTTGATACACATTACGCGa                                                                                                     <  4:142537/149‑1 (MQ=255)
                                                cttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGAc                                                                                                    <  4:891425/149‑1 (MQ=255)
                                                    gTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  4:1045058/130‑1 (MQ=255)
                                                          accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  4:806450/107‑1 (MQ=255)
                                                          accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  2:401962/149‑1 (MQ=255)
                                                           ccacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAg                                                                                         >  1:697077/1‑149 (MQ=255)
                                                            cacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATacac                                                                                                              <  4:551134/127‑1 (MQ=255)
                                                             acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         >  3:1634508/1‑99 (MQ=255)
                                                             acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         <  4:1634508/99‑1 (MQ=255)
                                                                 ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   >  1:1071576/1‑117 (MQ=255)
                                                                 ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  2:1071576/117‑1 (MQ=255)
                                                                 ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGAt                                                                                                                  >  3:1140513/1‑118 (MQ=255)
                                                                 ccGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGAt                                                                                                                  <  4:1140513/118‑1 (MQ=255)
                                                                   gCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGc                                                                                 >  4:6715/1‑149 (MQ=255)
                                                                     cTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  2:1204994/96‑1 (MQ=255)
                                                                      ttCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTACACCGGAGTTGTCg                                                                                   <  2:734495/144‑1 (MQ=255)
                                                                          gCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACAtt                                                                                                           >  4:267970/1‑116 (MQ=255)
                                                                          gCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACAtt                                                                                                           <  3:267970/116‑1 (MQ=255)
                                                                          gCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTa                                                                                                          <  3:865075/117‑1 (MQ=255)
                                                                              aTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCt                                                                                                                      >  3:845663/1‑101 (MQ=255)
                                                                               tCACCTTTTTTGACCTTACCACGCGGATTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCAt                                                                     >  1:943267/1‑149 (MQ=255)
                                                                               tCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGTCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCAt                                                                     >  1:1438743/1‑149 (MQ=255)
                                                                                 aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     <  3:1485806/99‑1 (MQ=255)
                                                                                 aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGAc                                                                              <  1:1563126/138‑1 (MQ=255)
                                                                                 aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAg                                                                   >  1:1782881/1‑149 (MQ=255)
                                                                                   cTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTc                                                                 >  4:484311/1‑149 (MQ=255)
                                                                                    ttttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                              <  2:273643/72‑3 (MQ=255)
                                                                                      ttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGt                                                              <  3:462363/149‑1 (MQ=255)
                                                                                       tttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGtt                                                             <  2:1138702/149‑1 (MQ=255)
                                                                                          gACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          <  2:1799209/149‑1 (MQ=255)
                                                                                           aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  2:1561105/149‑1 (MQ=255)
                                                                                           aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  1:554765/149‑1 (MQ=255)
                                                                                           aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  2:1314709/149‑1 (MQ=255)
                                                                                           aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTACGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         <  1:1807173/149‑1 (MQ=255)
                                                                                            ccTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTg                                                         >  3:1154029/1‑148 (MQ=255)
                                                                                             cTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  1:1481886/1‑149 (MQ=255)
                                                                                              ttACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGAt                                                      >  4:425383/1‑149 (MQ=255)
                                                                                                      cgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGAc                                                                              <  4:533411/117‑1 (MQ=255)
                                                                                                      cgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGAc                                                                              >  3:533411/1‑117 (MQ=255)
                                                                                                         aaTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGc                                           >  1:227553/1‑149 (MQ=255)
                                                                                                             gCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  3:634220/120‑1 (MQ=255)
                                                                                                              cttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      >  2:1572919/1‑149 (MQ=255)
                                                                                                              cttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      >  4:1210536/1‑149 (MQ=255)
                                                                                                               ttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    >  3:1546100/1‑118 (MQ=255)
                                                                                                               ttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     >  1:1208686/1‑149 (MQ=255)
                                                                                                                 cttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTaa                                   <  2:471191/149‑1 (MQ=255)
                                                                                                                  tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      >  3:242813/1‑145 (MQ=255)
                                                                                                                       tGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCaa                             <  2:697077/149‑1 (MQ=255)
                                                                                                                        ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  1:1109654/109‑1 (MQ=255)
                                                                                                                               ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          >  1:575896/1‑112 (MQ=255)
                                                                                                                               ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          <  2:575896/112‑1 (MQ=255)
                                                                                                                               ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactacta                       <  3:1830446/147‑1 (MQ=255)
                                                                                                                               ttGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactacta                       >  4:1830446/1‑147 (MQ=255)
                                                                                                                                 gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTga                   >  4:1686388/1‑149 (MQ=255)
                                                                                                                                    gatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         >  4:859637/1‑92 (MQ=255)
                                                                                                                                    gatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGAc                >  1:958317/1‑149 (MQ=255)
                                                                                                                                        tCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGa            >  3:554968/1‑149 (MQ=255)
                                                                                                                                         tcgcTACGCAGGCGTAGTCGACGGGGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGa            >  3:1621876/4‑149 (MQ=255)
                                                                                                                                            cTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAAt        >  3:540847/1‑149 (MQ=255)
                                                                                                                                              aCGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCa      <  3:1210536/149‑1 (MQ=255)
                                                                                                                                               cGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAg     <  2:95120/149‑1 (MQ=255)
                                                                                                                                                gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCAcc                                                                                             <  3:1781918/60‑1 (MQ=255)
                                                                                                                                                gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactactac                      >  2:1813147/1‑131 (MQ=255)
                                                                                                                                                  cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactacta                       >  4:1846028/1‑128 (MQ=255)
                                                                                                                                                  cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGAccc              <  4:1724346/137‑1 (MQ=255)
                                                                                                                                                  cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGAccc              >  3:1724346/1‑137 (MQ=255)
                                                                                                                                                  cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTc  >  4:321448/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447904‑3448198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: