Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,451,404 T→G 5.9% D94A (GAC→GCC)  rplW ← 50S ribosomal subunit protein L23

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,451,4040TG 5.9% 786.0 / 32.3 221D94A (GAC→GCC) rplW50S ribosomal subunit protein L23
Reads supporting (aligned to +/- strand):  ref base T (97/111);  new base G (8/5);  total (105/116)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 223 total aligned reads displayed.

TTTTTTCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGC  >  NC_000913/3451256‑3451552
                                                                                                                                                    |                                                                                                                                                    
   tttCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCa                                                                                                                                                   >  1:1369022/1‑149 (MQ=255)
     tCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGa                                                                                                                                                 <  2:645696/149‑1 (MQ=255)
              ggAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCct                                                                                                                                        <  3:394446/149‑1 (MQ=255)
                   aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGAtt                                                                                                                                               >  1:674731/1‑137 (MQ=255)
                   aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGAtt                                                                                                                                               <  2:674731/137‑1 (MQ=255)
                   aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGGCCAGAATCTgg                                                                                                                                           >  4:1192440/1‑141 (MQ=255)
                      aGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAg                                                                                                                                <  3:650755/149‑1 (MQ=255)
                       ggTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAgg                                                                                                                               <  2:1309904/149‑1 (MQ=255)
                       ggTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAgg                                                                                                                               <  4:562355/149‑1 (MQ=255)
                              ccTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTcc                                                                                                                                                    <  3:1872775/121‑1 (MQ=255)
                              ccTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGt                                                                                                                        <  1:1719757/149‑1 (MQ=255)
                              ccTTGTGCAGCTCAGGGTTAACACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTa                                                                                                                       >  4:566596/1‑149 (MQ=255)
                                ttGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTaa                                                                                                                      >  3:790689/1‑149 (MQ=255)
                                    gCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtt                                                                                                                  >  3:54354/1‑149 (MQ=255)
                                      aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                <  2:1678590/149‑1 (MQ=255)
                                      aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                <  2:1865021/149‑1 (MQ=255)
                                       gCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCttttt                                                                                                               <  3:1087618/149‑1 (MQ=255)
                                         tCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCcttct                                                                                                                                     >  3:1172894/1‑125 (MQ=255)
                                         tCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCcttct                                                                                                                                     <  4:1172894/125‑1 (MQ=255)
                                         tCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTc                                                                                                             >  2:1094929/1‑149 (MQ=255)
                                          cAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTcc                                                                                                            >  4:1063593/1‑149 (MQ=255)
                                           aGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                           <  4:801898/149‑1 (MQ=255)
                                           aGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                           <  3:270176/149‑1 (MQ=255)
                                                 aaCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                           <  3:602563/143‑1 (MQ=255)
                                                 aaCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                           >  4:602563/1‑143 (MQ=255)
                                                  aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTc                                                                                                             <  1:1465516/140‑1 (MQ=255)
                                                  aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTa                                                                                                    >  2:444864/1‑149 (MQ=255)
                                                  aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGGCCAGAATCTGGActtc                                                                                                                                      >  4:704234/1‑115 (MQ=255)
                                                   tcACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTg                                                                                                                                            <  4:528530/107‑1 (MQ=255)
                                                       tttAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAg                                                                                                                                <  4:431812/116‑1 (MQ=255)
                                                           aCTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCg                                                                                           >  1:1772944/1‑149 (MQ=255)
                                                            cTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTacga                                                                                                 >  1:501956/1‑142 (MQ=255)
                                                             tACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGAt                                                                                         >  1:528392/1‑149 (MQ=255)
                                                                 tGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                           <  2:1506886/127‑1 (MQ=255)
                                                                  ggCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCt                                                                                                                   >  4:184563/1‑118 (MQ=255)
                                                                    cgacgaCCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTg                                                                                   <  2:778881/148‑1 (MQ=255)
                                                                     gacgacCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAg                                                                                                                                                  <  3:45282/84‑1 (MQ=255)
                                                                     gacgacCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTc                                                                                 <  2:1758486/149‑1 (MQ=255)
                                                                      acgacCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCt                                                                                                                                             >  2:1251151/1‑88 (MQ=255)
                                                                      acgacCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTcc                                                                                <  1:1382047/149‑1 (MQ=255)
                                                                          cccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGa                                                                            <  3:1821974/149‑1 (MQ=255)
                                                                          cccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGa                                                                            <  3:566596/149‑1 (MQ=255)
                                                                          cccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGa                                                                            <  1:981615/149‑1 (MQ=255)
                                                                           ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCcttc                                                                                                                                      >  1:1169870/1‑90 (MQ=255)
                                                                           ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGc                                                                                                                    >  4:250101/1‑108 (MQ=255)
                                                                           ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATAc                                                                                       <  1:1326503/137‑1 (MQ=255)
                                                                           ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGAc                                                                           >  2:1797324/1‑149 (MQ=255)
                                                                             ggAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGt                                                                         <  4:750069/149‑1 (MQ=255)
                                                                              gagaTGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                >  2:1023570/1‑109 (MQ=255)
                                                                              gagaTGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                <  1:1023570/109‑1 (MQ=255)
                                                                               agaTGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                <  2:1376048/108‑1 (MQ=255)
                                                                                     cGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACttt                                                                 >  3:230569/1‑149 (MQ=255)
                                                                                      ggTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTc                                                                <  1:1094929/149‑1 (MQ=255)
                                                                                       gTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTcc                                                               <  3:917276/149‑1 (MQ=255)
                                                                                        tttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTg                                                                                                                                            >  1:169145/1‑71 (MQ=255)
                                                                                           aCATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTacga                                                                                                 <  2:933759/111‑1 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttttt                                                                                                              >  3:119493/1‑96 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttttt                                                                                                              <  4:119493/96‑1 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTcc                                                                                                            >  1:958705/1‑98 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTcc                                                                                                            <  2:958705/98‑1 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTacgacg                                                                                               >  3:1761108/1‑111 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTacgacg                                                                                               <  1:197346/111‑1 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                         >  1:1228029/1‑149 (MQ=255)
                                                                                             aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                         >  2:513011/1‑149 (MQ=255)
                                                                                              tttAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAAc                                                        >  4:464870/1‑149 (MQ=255)
                                                                                                 aacaacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGa                                                                                                                                                 >  3:295582/1‑57 (MQ=255)
                                                                                                  acaacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACgacgac                                                                                             >  1:1284241/1‑108 (MQ=255)
                                                                                                  acaacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACgacgac                                                                                             <  1:911789/108‑1 (MQ=255)
                                                                                                   caacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCa                                                   >  2:113237/1‑149 (MQ=255)
                                                                                                    aacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGa                                                                                          <  3:1690302/109‑1 (MQ=255)
                                                                                                    aacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATa                                                                                        <  3:1782820/111‑1 (MQ=255)
                                                                                                    aacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATa                                                                                        >  4:1782820/1‑111 (MQ=255)
                                                                                                    aacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAg                                                  >  1:941197/1‑149 (MQ=255)
                                                                                                     acTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGGCCAGAATCTGGActt                                                                                                                                       <  2:1350429/63‑1 (MQ=38)
                                                                                                      cTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTAc                                                     >  4:1458766/1‑144 (MQ=255)
                                                                                                      cTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTAc                                                     <  3:1458766/144‑1 (MQ=255)
                                                                                                          cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                         >  3:1013733/1‑136 (MQ=255)
                                                                                                            ttGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTaa                                          <  2:1697225/149‑1 (MQ=255)
                                                                                                             tGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAc                                         <  4:230569/149‑1 (MQ=255)
                                                                                                              gTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcg                                        >  1:790136/1‑149 (MQ=255)
                                                                                                                   aCTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttc                                   <  3:1021154/149‑1 (MQ=255)
                                                                                                                       ctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTAc                                                     <  2:1217811/127‑1 (MQ=255)
                                                                                                                           gACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcga                           <  4:1017108/149‑1 (MQ=255)
                                                                                                                           gACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcga                           >  1:690287/1‑149 (MQ=255)
                                                                                                                            aCTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcgac                          <  2:284240/149‑1 (MQ=255)
                                                                                                                              ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTc                                                                                                        >  4:1504521/1‑69 (MQ=255)
                                                                                                                              ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTc                                                                                                        <  3:1504521/69‑1 (MQ=255)
                                                                                                                              ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAg                                                  <  4:372194/123‑1 (MQ=255)
                                                                                                                              ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAc                                         >  4:1761219/1‑132 (MQ=255)
                                                                                                                                  tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCttt                                                           >  1:724545/1‑110 (MQ=255)
                                                                                                                                  tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCttt                                                           <  2:724545/110‑1 (MQ=255)
                                                                                                                                  tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                    <  3:464870/149‑1 (MQ=255)
                                                                                                                                  tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                    <  1:1592788/149‑1 (MQ=255)
                                                                                                                                           ccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTg           <  4:287985/149‑1 (MQ=255)
                                                                                                                                             aaCGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGTCTTCAAACAGt                <  4:170764/142‑1 (MQ=255)
                                                                                                                                             aaCGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                    >  3:74380/1‑138 (MQ=255)
                                                                                                                                             aaCGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGca         >  4:1522327/1‑149 (MQ=255)
                                                                                                                                             aaCGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGc          >  3:1193475/1‑148 (MQ=255)
                                                                                                                                                 aaGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTAcc                                                    >  1:1832534/1‑102 (MQ=255)
                                                                                                                                                 aaGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCAcagc     >  1:430097/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTTTTCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGC  >  NC_000913/3451256‑3451552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: