Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,594,071 T→C 5.4% T46A (ACG→GCG)  livG ← branched‑chain amino acid ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,594,0710TC 5.4% 618.0 / 14.1 180T46A (ACG→GCG) livGbranched‑chain amino acid ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (90/80);  new base C (4/6);  total (94/86)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 188 total aligned reads displayed.

ATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGCTCTCCTT  >  NC_000913/3593923‑3594219
                                                                                                                                                    |                                                                                                                                                    
aTTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGt                                                                                                                                                      <  1:1809101/149‑1 (MQ=255)
     aCGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGttt                                                                                                                                                 >  2:464632/1‑149 (MQ=255)
     aCGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGttt                                                                                                                                                 >  4:1670191/1‑149 (MQ=255)
     aCGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGttt                                                                                                                                                 >  4:1851556/1‑149 (MQ=255)
        gAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGCGGTTTTTc                                                                                                                                              <  4:159117/149‑1 (MQ=255)
         aaCAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTcc                                                                                                                                             >  3:587063/1‑149 (MQ=255)
          aCAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaac                                                                                                                                            <  4:1701713/149‑4 (MQ=255)
           cAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaact                                                                                                                                           <  2:15729/149‑5 (MQ=255)
               cGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCGAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCAcc                                                                                                                                       <  3:1670191/149‑1 (MQ=255)
                    aTGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGt                                                                                                                                                      >  2:1191367/1‑129 (MQ=255)
                    aTGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                                                                  <  3:289779/149‑1 (MQ=255)
                     tGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAgg                                                                                                                                 <  4:1122965/149‑1 (MQ=255)
                        tGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTc                                                                                                                                              <  3:1745773/133‑1 (MQ=255)
                        tGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGcc                                                                                                                              >  3:1311986/1‑149 (MQ=255)
                        tGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGcc                                                                                                                              >  2:1518921/1‑149 (MQ=255)
                           aaGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAt                                                                                                                           <  3:1851556/149‑1 (MQ=255)
                            aGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAtt                                                                                                                          >  2:1582628/1‑149 (MQ=255)
                               tGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAAc                                                                                                                       <  4:587063/149‑1 (MQ=255)
                                  gcACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACgag                                                                                                                    >  4:1580391/1‑149 (MQ=255)
                                                 gggCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAgg                                                                                                                                 <  1:1161050/121‑1 (MQ=255)
                                                 gggCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCggg                                                                                                     >  2:584927/1‑149 (MQ=255)
                                                     aaTTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACa                                                                                                 >  2:1538455/1‑149 (MQ=255)
                                                       tttGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTcc                                                                                                           >  3:1227328/1‑137 (MQ=255)
                                                       tttGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTcc                                                                                                           <  4:1227328/137‑1 (MQ=255)
                                                       tttGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGt                                                                                               >  3:656673/1‑149 (MQ=255)
                                                        ttGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGtt                                                                                              >  1:723086/1‑149 (MQ=255)
                                                          gctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCa                                                                                            >  2:225796/1‑149 (MQ=255)
                                                           ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                                                                           <  4:175275/149‑1 (MQ=255)
                                                           ctgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                                                                           <  1:308755/149‑1 (MQ=255)
                                                            tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGtt                                                                                                                                                  <  2:1221020/93‑1 (MQ=255)
                                                            tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGtt                                                                                                                                                  >  1:1221020/1‑93 (MQ=255)
                                                            tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAt                                                                                                                           >  1:424396/1‑116 (MQ=255)
                                                            tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGAt                                                                                                                           <  2:424396/116‑1 (MQ=255)
                                                            tgcCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAg                                                                                          >  2:86346/1‑149 (MQ=255)
                                                                ccGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCACGGtttttaa                                                                                                                                             <  4:276676/94‑3 (MQ=255)
                                                                   gTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCa                                                                                                                                         >  4:382462/1‑95 (MQ=255)
                                                                   gTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCa                                                                                                                                         <  3:382462/95‑1 (MQ=255)
                                                                      caCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttg                                                                                <  3:1844130/148‑1 (MQ=255)
                                                                       aCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt                                                                               <  4:1311986/149‑1 (MQ=255)
                                                                       aCCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt                                                                               <  1:1518921/149‑1 (MQ=255)
                                                                        ccTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTaa                                                                                                                        >  1:461820/1‑107 (MQ=255)
                                                                          ttCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                                                                  >  2:173787/1‑95 (MQ=255)
                                                                          ttCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAg                                                                                                                                  <  1:173787/95‑1 (MQ=255)
                                                                          ttCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTaa                                                                                                                        <  3:592242/105‑1 (MQ=255)
                                                                                    tGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagcag                                                                  >  3:355079/1‑149 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTaa                                                                                                                        >  4:635863/1‑94 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                                                                           >  1:1297182/1‑123 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCaa                                                                                           <  2:1297182/123‑1 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAcc                                                                          <  1:236174/140‑1 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCAc                                                                           >  2:648081/1‑139 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAgg                                                                 >  4:1074031/1‑149 (MQ=255)
                                                                                     gATCGCGCAGTAAAATGGTGCCGCCGATGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAgg                                                                 >  2:1194448/1‑149 (MQ=255)
                                                                                            cAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGa                                                          >  3:1797342/1‑149 (MQ=255)
                                                                                             aGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGaa                                                         <  3:1074031/149‑1 (MQ=255)
                                                                                              gTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAg                                                        >  4:509379/1‑149 (MQ=255)
                                                                                               tAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCca                                                                      >  4:34515/1‑134 (MQ=255)
                                                                                               tAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgc                                                       <  4:1797342/149‑1 (MQ=255)
                                                                                               tAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgc                                                       >  1:330943/1‑149 (MQ=255)
                                                                                                 aaaTGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAgcgc                                                     <  4:656673/149‑1 (MQ=255)
                                                                                                         ccgccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAg                                             <  3:509379/149‑1 (MQ=255)
                                                                                                         ccgccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAg                                             <  2:330943/149‑1 (MQ=255)
                                                                                                           gccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGc                                           <  3:1580391/149‑1 (MQ=255)
                                                                                                            ccgGTGGGTTTGTAGAATCCGGTCAGACAGTTAATAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGGTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGcc                                          <  1:1538455/149‑1 (MQ=255)
                                                                                                                tGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca                                                                   <  3:717597/120‑1 (MQ=255)
                                                                                                                tGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagca                                                                   >  4:717597/1‑120 (MQ=255)
                                                                                                                 gggTTTGTCGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCg                                         <  2:1066623/145‑1 (MQ=255)
                                                                                                                   gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTg                                                                                                         >  3:1063095/1‑79 (MQ=255)
                                                                                                                   gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTg                                                                                                         <  4:1063095/79‑1 (MQ=255)
                                                                                                                   gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTg                                                                                                         <  2:510565/79‑1 (MQ=255)
                                                                                                                   gTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGcatcat                                                >  2:1322888/1‑136 (MQ=255)
                                                                                                                    tttGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAg                                             >  3:1410427/1‑138 (MQ=255)
                                                                                                                    tttGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAg                                             <  4:1410427/138‑1 (MQ=255)
                                                                                                                     ttGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGa                                 >  3:322450/1‑149 (MQ=255)
                                                                                                                      tGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGat                                <  4:322450/149‑1 (MQ=255)
                                                                                                                         aGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGataat                             >  1:930190/1‑149 (MQ=255)
                                                                                                                          gAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAAc                                    <  3:101162/141‑1 (MQ=255)
                                                                                                                          gAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAAc                                    >  4:101162/1‑141 (MQ=255)
                                                                                                                            aTCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGaa                                                         <  2:307577/118‑1 (MQ=255)
                                                                                                                             tCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGAtctc                                                                                                            <  1:103966/66‑1 (MQ=255)
                                                                                                                                  tCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCcagcag                                                                  <  1:645811/103‑1 (MQ=255)
                                                                                                                                         gTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGAc                                                                                    <  1:1547299/78‑1 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                                                                 <  2:511276/80‑1 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgtt                                                                                 >  1:511276/1‑80 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTACCAGCTAATAATGGCTGACTCATGcc            >  1:1667977/1‑149 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATg                         >  1:488091/1‑136 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGcc            >  4:1505322/1‑149 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGcc            >  3:76118/1‑149 (MQ=255)
                                                                                                                                          ttAAAAACCGTGGTTTTTCCGGCACCGGTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCACCAGGCCGTTAACAGATAATAATg                         >  3:1260933/1‑136 (MQ=255)
                                                                                                                                            aaaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTaa                                     >  4:302023/1‑122 (MQ=255)
                                                                                                                                            aaaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTg          <  1:1194448/149‑1 (MQ=255)
                                                                                                                                             aaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt                                                                               >  1:1360804/1‑79 (MQ=255)
                                                                                                                                             aaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACgttgt                                                                               <  2:1360804/79‑1 (MQ=255)
                                                                                                                                             aaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGc         <  3:1529685/149‑1 (MQ=255)
                                                                                                                                             aaaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGc         <  1:86346/149‑1 (MQ=255)
                                                                                                                                              aaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGc             >  4:940677/1‑144 (MQ=255)
                                                                                                                                              aaaCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGc             <  3:940677/144‑1 (MQ=255)
                                                                                                                                                aCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCt                      <  3:1038271/133‑1 (MQ=255)
                                                                                                                                                aCCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCt                      >  4:1038271/1‑133 (MQ=255)
                                                                                                                                                   gTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGCTCTCCt   >  1:1015990/1‑149 (MQ=255)
                                                                                                                                                   gTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGCTCTCCt   >  4:1282730/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTTCACGGAACAGACGCACATGCTGGAAGGTGCGCACCACGCCCATGCGGGCAATTTGCTGCCCCGGTAAACCTTCCAGGTGCTGATCGCGCAGTAAAATGGTGCCGCCGGTGGGTTTGTAGAATCCGGTCAGACAGTTAAAAACCGTGGTTTTTCCGGCACCGTTAGGGCCGATTAACGAGACGATCTCCTGCGGGTACAGTTCAAGATTGACGTTGTTCACCGCCAGCAGGCCGCCGAAGCGCATCATCAGGCCGTTAACAGATAATAATGGCTGACTCATGCCTGCTCTCCTT  >  NC_000913/3593923‑3594219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: