| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,620,417 | G→T | 6.3% | R409L (CGG→CTG) | rhsB → | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,620,417 | 0 | G | T | 6.3% | 223.4 / 4.7 | 63 | R409L (CGG→CTG) | rhsB | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base G (29/30); new base T (2/2); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACG > NC_000913/3620272‑3620565 | tACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 2:1486987‑M2/1‑149 (MQ=255) aCGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 2:1350118‑M2/1‑149 (MQ=255) cGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG > 2:828302‑M2/1‑149 (MQ=255) gACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT > 1:718922‑M2/1‑149 (MQ=255) aCAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG < 1:28019‑M2/149‑1 (MQ=255) aCAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG < 1:1535035‑M2/149‑1 (MQ=255) aCAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG > 1:1820414‑M2/1‑149 (MQ=255) aCAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCAGTGAAGTACTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGGGGTG < 4:1595313‑M2/149‑1 (MQ=255) cAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGA < 4:1425553‑M2/149‑1 (MQ=255) cGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG < 1:1093183‑M2/142‑1 (MQ=255) cGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG < 1:1115574‑M2/142‑1 (MQ=255) cGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 2:1115574‑M2/1‑142 (MQ=255) cGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 2:1093183‑M2/1‑142 (MQ=255) gcgggTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACGCGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT > 2:276802‑M2/1‑138 (MQ=255) gcgggTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT < 1:276802‑M2/138‑1 (MQ=255) ggTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG < 2:24368‑M2/136‑1 (MQ=255) ggTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG > 1:24368‑M2/1‑136 (MQ=255) gTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCGCACGCAGGGCGAAGCCGGGCTGAAGCGGGTG < 1:1692961‑M2/132‑1 (MQ=255) gTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCGCACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 2:1692961‑M2/1‑132 (MQ=255) tGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT < 4:171802‑M2/130‑1 (MQ=255) tGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 3:171802‑M2/1‑130 (MQ=255) cTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG < 4:308021‑M2/120‑1 (MQ=255) cTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 3:308021‑M2/1‑120 (MQ=255) tAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG > 2:1217964‑M2/1‑120 (MQ=255) tAAACCCGGCAGGCTTAAGCTACACGTATCAGGATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGAGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG < 1:1217964‑M2/120‑1 (MQ=255) aaCCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT > 4:801236‑M2/1‑119 (MQ=255) aaCCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT < 3:801236‑M2/119‑1 (MQ=255) aCCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG < 4:1516043‑M2/117‑1 (MQ=255) aCCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG > 3:1516043‑M2/1‑117 (MQ=255) ccGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 4:371436‑M2/1‑113 (MQ=255) ccGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT < 3:371436‑M2/113‑1 (MQ=255) aGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG < 1:1067755‑M2/110‑1 (MQ=255) aGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG > 2:1067755‑M2/1‑110 (MQ=255) ttAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG > 1:97320‑M2/1‑105 (MQ=255) ttAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTG < 2:97320‑M2/105‑1 (MQ=255) tACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT < 3:1551723‑M2/98‑1 (MQ=255) tACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT < 1:1031647‑M2/98‑1 (MQ=255) tACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 4:1551723‑M2/1‑98 (MQ=255) tACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 2:1031647‑M2/1‑98 (MQ=255) acacGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG > 3:1669288‑M2/1‑101 (MQ=255) acacGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTG < 4:1669288‑M2/101‑1 (MQ=255) acGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG < 1:233920‑M2/97‑1 (MQ=255) acGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGG > 2:233920‑M2/1‑97 (MQ=255) gTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT > 4:801970‑M2/1‑96 (MQ=255) gTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT < 3:801970‑M2/96‑1 (MQ=255) gACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgccc < 3:1303230‑M2/149‑68 (MQ=255) atcaccatcaccGACAGCCTGGACCGCGGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgct < 2:358993‑M2/149‑74 (MQ=255) atcaccatcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgct < 1:1434590‑M2/149‑74 (MQ=255) caccatcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctctctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgt < 2:710622‑M2/149‑76 (MQ=255) ccatcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT > 1:1575894‑M2/1‑67 (MQ=255) ccatcaccGACAGCCTGGACCGCCGGGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGT < 2:1575894‑M2/67‑1 (MQ=255) catcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgtcgt < 1:653212‑M2/149‑79 (MQ=255) catcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgt > 4:862898‑M2/1‑71 (MQ=255) catcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgt < 3:862898‑M2/124‑54 (MQ=255) tcaccGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgtcgtaa > 2:653212‑M2/1‑69 (MQ=255) cAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgtcgtaacggtaac > 4:1303230‑M2/1‑62 (MQ=255) cTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGTCGGTGATGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagct < 3:489648‑M2/109‑52 (MQ=255) cTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGTCGGTGATGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagct > 4:489648‑M2/1‑58 (MQ=255) cTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT < 2:1059373‑M2/56‑1 (MQ=255) cTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGT > 1:1059373‑M2/1‑56 (MQ=255) ggACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGTCGGTGATGGTGAtgcggtctttctca < 4:1018885‑M2/70‑15 (MQ=255) ggACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGTCGGTGATGGTGAtgcggtctttctca > 3:1018885‑M2/1‑56 (MQ=255) aGCCGGGCTGAAGCGGGTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgtcgtaacggtaacggatttccggccggcccgtgtgacggtgcgccaccatcc > 1:710622‑M2/1‑23 (MQ=255) gggTGGTGAtgcggtctttctcatactgatacgtgtagcttaagcctgccgggtttagctgttctgtcacccgcccgtcgctgtcgtaacggtaacggatttccggccggcccgtgtgacggtgcgccaccatccggccccggtattta > 1:358993‑M2/1‑9 (MQ=255) | TACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACG > NC_000913/3620272‑3620565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |