Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,676,195 A→G 5.3% intergenic (+87/+95) yhjE → / ← yhjG putative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,1950AG 5.3% 263.1 / 3.4 76intergenic (+87/+95)yhjE/yhjGputative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base G (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

GCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCT  >  NC_000913/3676049‑3676338
                                                                                                                                                  |                                                                                                                                               
gctggctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  1:1067248/149‑1 (MQ=255)
gctggctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  1:610872/149‑1 (MQ=255)
gctggctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  2:1288136/149‑1 (MQ=255)
  tggctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGc                                                                                                                                             <  2:493709/149‑1 (MQ=255)
         aCGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               >  2:614402/1‑140 (MQ=255)
         aCGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  1:614402/140‑1 (MQ=255)
          cGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                                     <  2:720177/149‑1 (MQ=255)
          cGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                                     <  3:25912/149‑1 (MQ=255)
           gTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                    >  4:1617747/1‑149 (MQ=255)
             tAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                  <  4:1886629/149‑1 (MQ=255)
                   cccTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                    <  4:246104/141‑1 (MQ=255)
                   cccTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                    >  3:246104/1‑141 (MQ=255)
                             gACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaaa                                                                                                                  <  3:1617747/149‑1 (MQ=255)
                                 cATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcac                                                                                                              >  4:1088202/1‑149 (MQ=255)
                                  aTGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGc                                                                                                                                             <  2:1004613/117‑1 (MQ=255)
                                  aTGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGc                                                                                                                                             >  1:1004613/1‑117 (MQ=255)
                                       aCGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  3:11191/110‑1 (MQ=255)
                                       aCGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               >  4:11191/1‑110 (MQ=255)
                                       aCGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTg                                                                                                        >  3:1634194/1‑149 (MQ=255)
                                           gACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaa                                                                                                    <  4:1634194/149‑1 (MQ=255)
                                             cATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcaca                                                                                                             >  3:1662334/1‑138 (MQ=255)
                                             cATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcaca                                                                                                             <  4:1662334/138‑1 (MQ=255)
                                                  gTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGcc                                                                                                      >  3:1137850/1‑140 (MQ=255)
                                                  gTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGcc                                                                                                      <  4:1137850/140‑1 (MQ=255)
                                                    cGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTaaa                                                                                           <  3:1088202/149‑1 (MQ=255)
                                                      ttGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                           <  2:1704240/115‑1 (MQ=37)
                                                      ttGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                           >  1:1704240/1‑115 (MQ=37)
                                                       tGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTg                                                                                                        <  3:689929/133‑1 (MQ=255)
                                                       tGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTg                                                                                                        >  4:689929/1‑133 (MQ=255)
                                                         taataaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               <  3:1504051/92‑1 (MQ=255)
                                                         taataaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAg                                                                                                                                               >  4:1504051/1‑92 (MQ=255)
                                                           ataaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGc                                                                                                       <  4:1645351/130‑1 (MQ=255)
                                                           ataaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGc                                                                                                       >  3:1645351/1‑130 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTgtt                                                                                                      >  3:453659/1‑128 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTgtt                                                                                                      <  4:453659/130‑3 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaacaa                                                                                                 <  3:902630/135‑1 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaacaa                                                                                                 >  4:902630/1‑135 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATc                                                                                               <  3:1783171/137‑1 (MQ=255)
                                                            taaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATc                                                                                               >  4:1783171/1‑137 (MQ=255)
                                                               gagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACtt                                                                                                          <  2:1225462/123‑1 (MQ=255)
                                                               gagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACtt                                                                                                          >  1:1225462/1‑123 (MQ=255)
                                                               gagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaac                                                                                                   >  2:1310748/1‑130 (MQ=255)
                                                               gagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaac                                                                                                   <  1:1310748/130‑1 (MQ=255)
                                                                     aaaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATc                                                                                         >  2:366174/1‑134 (MQ=255)
                                                                     aaaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATc                                                                                         <  1:366174/134‑1 (MQ=255)
                                                                         ccTGATGCGCTACGCTTCTCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg                                                                                                                 >  2:627751/1‑106 (MQ=255)
                                                                         ccTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg                                                                                                                 <  1:627751/106‑1 (MQ=255)
                                                                                 gcTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcaca                                                                                                             <  4:312198/102‑1 (MQ=255)
                                                                                 gcTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcaca                                                                                                             >  3:312198/1‑102 (MQ=255)
                                                                                   tACGCTTATCAGGCCTGAATTTCCATACACTATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCa                                                                     <  4:146230/140‑1 (MQ=255)
                                                                                   tACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCa                                                                     >  3:146230/1‑140 (MQ=255)
                                                                                        ttATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGtt                                                                                     >  4:1313120/1‑119 (MQ=255)
                                                                                        ttATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGtt                                                                                     <  3:1313120/119‑1 (MQ=255)
                                                                                          aTCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaa                                                                                                    >  4:1474226/1‑102 (MQ=255)
                                                                                          aTCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaa                                                                                                    <  3:1474226/102‑1 (MQ=255)
                                                                                                      tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAAt                                                                                 <  1:697168/109‑1 (MQ=255)
                                                                                                      tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAAt                                                                                 >  2:697168/1‑109 (MQ=255)
                                                                                                           aTACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGc                                                                              <  4:1399488/107‑1 (MQ=255)
                                                                                                           aTACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGc                                                                              >  3:1399488/1‑107 (MQ=255)
                                                                                                                     ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATc                                                               >  2:815198/1‑112 (MQ=255)
                                                                                                                     ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATc                                                               <  1:815198/112‑1 (MQ=255)
                                                                                                                     ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAtcatca                                                >  2:229201/1‑127 (MQ=255)
                                                                                                                     ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAtcatca                                                <  1:229201/127‑1 (MQ=255)
                                                                                                                             gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             >  4:415569/1‑90 (MQ=255)
                                                                                                                             gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             <  3:415569/90‑1 (MQ=255)
                                                                                                                                aGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGtt                                                                                     <  1:900248/79‑1 (MQ=255)
                                                                                                                                aGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGtt                                                                                     >  2:900248/1‑79 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             <  1:305317/84‑1 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             <  1:1688587/84‑1 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             >  2:305317/1‑84 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                             >  2:1688587/1‑84 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCg                                                             <  2:671769/100‑1 (MQ=255)
                                                                                                                                   aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCg                                                             >  1:671769/1‑100 (MQ=255)
                                                                                                                                    tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTa           >  3:754277/1‑149 (MQ=255)
                                                                                                                                             cGGATGCGGCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATcc                                                              <  3:261690/89‑1 (MQ=25)
                                                                                                                                             cGGATGCGGCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCt  >  1:1121424/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCT  >  NC_000913/3676049‑3676338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: