| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,877,558 | G→C | 60.0% | intergenic (‑93/+147) | yidB ← / ← gyrB | DUF937 family protein/DNA gyrase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,877,558 | 0 | G | C | 60.0% | 1.3 / 9.9 | 10 | intergenic (‑93/+147) | yidB/gyrB | DUF937 family protein/DNA gyrase, subunit B |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base C (3/3); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
ATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGC > NC_000913/3877411‑3877566 | atTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCATCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatga < 3:807903/149‑1 (MQ=255) tttCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc < 1:836681/140‑1 (MQ=255) tttCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc > 2:836681/1‑140 (MQ=255) aGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatga < 1:1431014/129‑1 (MQ=255) aGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatga > 2:1431014/1‑129 (MQ=255) aCAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtc > 1:568490/1‑119 (MQ=255) aCAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtc < 2:568490/119‑1 (MQ=255) cTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat < 3:970581/114‑1 (MQ=255) cTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAgatgat > 4:970581/1‑114 (MQ=255) ttCATCAAAAAGCCCCGTGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc > 1:984162/1‑119 (MQ=255) ttCATCAAAAAGCCCCGTGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc < 2:984162/119‑1 (MQ=255) ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc > 3:1517676/1‑119 (MQ=255) ttCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATCATCTCCGc < 4:1517676/119‑1 (MQ=255) aaGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtct < 3:1062377/83‑1 (MQ=255) aaGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGAtct > 4:1062377/1‑83 (MQ=255) | ATTTCCCCGCATCGCCTTTCAGAAAGGCACCGACAACTTCATCAAAAAGCCCCATGATAATCACCTGTAAAGCGTTACGTGTTGACCCAAAAAGTATAGATTTGCGGGATGATAATTGCGGATTGCAGAAATAAAAAGGGCGGAGATGATCTCCGC > NC_000913/3877411‑3877566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |