Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,891,504 T→A 10.2% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,5040TA10.2% 394.3 / 26.8 117intergenic (+21/‑111)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (53/52);  new base A (6/6);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 119 total aligned reads displayed.

CCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTT  >  NC_000913/3891361‑3891652
                                                                                                                                               |                                                                                                                                                    
cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                                                                 <  3:240669/149‑1 (MQ=255)
cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                                                                 <  4:1800070/149‑1 (MQ=255)
     tCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                            <  4:560709/149‑1 (MQ=255)
        gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt                                                                                                                                         <  3:52958/149‑1 (MQ=255)
            ttCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCct                                                                                                                                     >  1:1579164/1‑149 (MQ=255)
               aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                                                                 >  3:1861879/1‑134 (MQ=255)
               aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCt                                                                                                                                                 <  4:1861879/134‑1 (MQ=255)
                       gggCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                                                                          >  2:147831/1‑149 (MQ=255)
                             cAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                    <  1:755867/149‑1 (MQ=255)
                              aaTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATg                                                                                                                   >  3:1554549/1‑149 (MQ=255)
                                tCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                                                                 >  3:921391/1‑149 (MQ=255)
                                  ttATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAAt                                                                                                                           >  2:1798918/1‑137 (MQ=255)
                                     ttCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAAt                                                                                                            <  2:1579164/149‑1 (MQ=255)
                                      tCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATg                                                                                                           <  4:921391/149‑1 (MQ=255)
                                            gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttc                                                                                                                                           >  4:1118771/1‑111 (MQ=255)
                                            gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttc                                                                                                                                           <  3:1118771/111‑1 (MQ=255)
                                            gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       >  1:51873/1‑131 (MQ=255)
                                            gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       <  2:51873/131‑1 (MQ=255)
                                              ttGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                            <  2:249225/108‑1 (MQ=255)
                                              ttGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                            >  1:249225/1‑108 (MQ=255)
                                                       tctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttctt                                                                                                                                         >  1:485895/1‑102 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGc                                                                                                                                                  >  1:589227/1‑91 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGc                                                                                                                                                  <  2:589227/91‑1 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt                                                                                                                      >  4:59886/1‑119 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt                                                                                                                      <  3:59886/119‑1 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCaa                                                                                                       >  1:1605754/1‑134 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCaa                                                                                                       <  2:1605754/134‑1 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctc                                                                                         <  4:424447/148‑1 (MQ=255)
                                                         tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctc                                                                                         >  3:424447/1‑148 (MQ=255)
                                                              tataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGaa                                                                                                                             <  2:1380484/107‑1 (MQ=255)
                                                              tataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGaa                                                                                                                             >  1:1380484/1‑107 (MQ=255)
                                                                taACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                        <  4:673629/94‑1 (MQ=255)
                                                                taACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                        >  3:673629/1‑94 (MQ=255)
                                                                    cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAATGGCTGGcttc                                                                                                                                           >  4:1109344/1‑87 (MQ=38)
                                                                    cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                     <  4:20976/109‑1 (MQ=255)
                                                                    cGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                     >  3:20976/1‑109 (MQ=255)
                                                                     gTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                            <  4:1855383/85‑1 (MQ=255)
                                                                     gTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGctt                                                                                                                                            <  4:937035/85‑1 (MQ=255)
                                                                     gTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGcc                                                                                                 <  2:717171/128‑1 (MQ=255)
                                                                      tGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCaa                                                                                                       <  2:1086203/121‑1 (MQ=255)
                                                                      tGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCaa                                                                                                       >  1:1086203/1‑121 (MQ=255)
                                                                         ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctc                                                                                         <  2:1766775/132‑1 (MQ=255)
                                                                         ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctc                                                                                         >  1:1766775/1‑132 (MQ=255)
                                                                            tgtgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGc                                                                     <  1:147831/149‑1 (MQ=255)
                                                                                         cAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTAt                                                                            >  4:886582/1‑129 (MQ=255)
                                                                                         cAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTAt                                                                            <  3:886582/129‑1 (MQ=255)
                                                                                          aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                                                                              >  4:575220/1‑62 (MQ=37)
                                                                                          aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                                                                              <  4:1647893/62‑1 (MQ=37)
                                                                                          aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                                                                              <  3:575220/62‑1 (MQ=37)
                                                                                          aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCGAAtggctggc                                                                                                                                              >  3:1647893/1‑62 (MQ=37)
                                                                                           aCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       >  3:620300/1‑84 (MQ=255)
                                                                                           aCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       <  4:620300/84‑1 (MQ=255)
                                                                                           aCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTGCAAGAAGCCAGCGAATggctggct                                                                                                                                             >  3:1473070/1‑62 (MQ=25)
                                                                                           aCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTGCAAGAAGCCAGCGAATggctggct                                                                                                                                             <  4:1473070/62‑1 (MQ=25)
                                                                                              tCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                             <  1:667942/107‑1 (MQ=255)
                                                                                              tCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                             >  2:667942/1‑107 (MQ=255)
                                                                                              tCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACt                                                          >  3:447381/1‑142 (MQ=255)
                                                                                              tCTTTAACGTGTTAACTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGa                                                   >  3:132172/1‑149 (MQ=255)
                                                                                                tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGc                                                                                                                                                  >  2:896591/1‑52 (MQ=255)
                                                                                                tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGc                                                                                                                                                  <  1:896591/52‑1 (MQ=255)
                                                                                                tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGcc                                                                                                 <  4:613245/101‑1 (MQ=255)
                                                                                                tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTAc                                                           >  2:460054/1‑139 (MQ=255)
                                                                                                 ttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGc                                                <  4:1554549/149‑1 (MQ=255)
                                                                                                      gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTc                                                                                                                                                    >  1:1172504/1‑44 (MQ=255)
                                                                                                      gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTc                                                                                                                                                    <  2:1172504/44‑1 (MQ=255)
                                                                                                      gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtc                                                                                            >  2:1445649/1‑100 (MQ=255)
                                                                                                      gtgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtc                                                                                            <  1:1445649/100‑1 (MQ=255)
                                                                                                       tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATg                                                                                                           >  1:19233/1‑84 (MQ=255)
                                                                                                       tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCt                                               <  2:474926/144‑1 (MQ=255)
                                                                                                       tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCt                                               >  1:474926/1‑144 (MQ=255)
                                                                                                         ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAg                                                     <  2:1363761/136‑1 (MQ=255)
                                                                                                         ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAg                                                     >  1:1363761/1‑136 (MQ=255)
                                                                                                           aCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                     <  3:1024604/70‑1 (MQ=255)
                                                                                                           aCCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                     >  4:1024604/1‑70 (MQ=255)
                                                                                                              tAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTc                                                                                     >  1:748643/1‑99 (MQ=255)
                                                                                                              tAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTc                                                                                     <  2:748643/99‑1 (MQ=255)
                                                                                                                   ttGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCt                              <  3:638864/149‑1 (MQ=255)
                                                                                                                      gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       <  1:800868/57‑1 (MQ=255)
                                                                                                                      gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       >  2:800868/1‑57 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                                                                 <  4:18821/105‑1 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                                                                 <  4:937862/105‑1 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                                                                 >  3:937862/1‑105 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCa                                                                 >  3:18821/1‑105 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttttt                          >  1:1525765/1‑144 (MQ=255)
                                                                                                                            tCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttttt                          <  2:1525765/144‑1 (MQ=255)
                                                                                                                                 cAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCttt                                                                       >  2:408136/1‑94 (MQ=38)
                                                                                                                                 cAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCttt                                                                       <  1:408136/94‑1 (MQ=255)
                                                                                                                                    gaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                             >  2:270987/1‑69 (MQ=255)
                                                                                                                                    gaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                             <  1:270987/69‑1 (MQ=255)
                                                                                                                                     aagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGc                                          >  4:297089/1‑119 (MQ=255)
                                                                                                                                     aagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGc                                          <  3:297089/119‑1 (MQ=255)
                                                                                                                                        ccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGccc         >  1:1706870/1‑149 (MQ=255)
                                                                                                                                          agccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACt                                                                                                                       <  3:978944/37‑1 (MQ=255)
                                                                                                                                          agccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGc                                                >  2:359954/1‑108 (MQ=255)
                                                                                                                                          agccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGc                                                <  1:359954/108‑1 (MQ=255)
                                                                                                                                              aTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAgg                                                    <  3:1860132/100‑1 (MQ=255)
                                                                                                                                              aTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTATCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGttt   >  3:1088341/1‑149 (MQ=255)
                                                                                                                                              aTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGttt   >  1:593990/1‑149 (MQ=255)
                                                                                                                                               ttCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGtttt  >  2:926682/1‑149 (MQ=255)
                                                                                                                                               ttCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGtttt  >  4:1375945/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTT  >  NC_000913/3891361‑3891652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: