Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,442 G→T 5.0% intergenic (‑76/+107) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,4420GT 5.0% 651.5 / 18.0 179intergenic (‑76/+107)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (83/87);  new base T (3/6);  total (86/93)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01

Only 100 of 183 total aligned reads displayed.

TGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGC  >  NC_000913/3908296‑3908582
                                                                                                                                                  |                                                                                                                                            
tGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCa                                                                                                                                            >  3:777712/1‑149 (MQ=255)
    aTTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                                        <  4:777712/149‑1 (MQ=255)
     tttGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCgg                                                                                                                                       >  2:171027/1‑149 (MQ=255)
      ttGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCGGc                                                                                                                                      <  2:1669413/149‑1 (MQ=255)
            tagtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                                >  3:1211723/1‑149 (MQ=255)
             agtagAAGTTCAAATTACGAAGCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtgt                                                                                                                               <  4:1784582/149‑1 (MQ=255)
             agtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtgt                                                                                                                               <  1:171027/149‑1 (MQ=255)
              gtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGtt                                                                                                                              >  1:1132111/1‑149 (MQ=255)
               tagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTa                                                                                                                             >  2:847805/1‑149 (MQ=255)
               tagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTa                                                                                                                             <  1:1552880/149‑1 (MQ=255)
               tagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTa                                                                                                                             <  1:1635448/149‑1 (MQ=255)
                  aaGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAAcc                                                                                                                          <  1:847805/149‑1 (MQ=255)
                    gTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgt                                                                                                                        <  2:1334668/149‑1 (MQ=255)
                    gTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgt                                                                                                                        >  4:1018101/1‑149 (MQ=255)
                     ttCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                                        <  1:101501/132‑1 (MQ=255)
                     ttCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                                        >  4:1245647/1‑132 (MQ=255)
                     ttCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgtg                                                                                                                       >  4:1653538/1‑149 (MQ=255)
                           ttACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACaga                                                                                                                 <  3:69876/149‑1 (MQ=255)
                               gAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATACGGCAAAGTGTTAACCGTGTACAGAGTCa                                                                                                             >  2:110104/1‑149 (MQ=255)
                                 aCCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCaaa                                                                                                           >  1:514799/1‑149 (MQ=255)
                                   cTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCGGc                                                                                                                                      <  4:818164/120‑1 (MQ=38)
                                   cTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCaaaaa                                                                                                         >  1:1390156/1‑149 (MQ=255)
                                    tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATcc                                                                                                                                         <  3:281372/116‑1 (MQ=255)
                                    tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATcc                                                                                                                                         >  4:281372/1‑116 (MQ=255)
                                      aaTTTTACTCGGGGCATTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgc                                                                                                      <  4:1704074/149‑1 (MQ=255)
                                      aaTTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgc                                                                                                      <  2:1132111/149‑1 (MQ=255)
                                      aaTTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgc                                                                                                      <  2:1390156/149‑1 (MQ=255)
                                                 gggCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTcggtcggt                                                                                           <  1:110104/149‑1 (MQ=255)
                                                  ggCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCggtcggtc                                                                                          >  3:1268010/1‑149 (MQ=255)
                                                   gCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgcgc                                                                                                    >  3:767353/1‑138 (MQ=255)
                                                           aaCCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgtg                                                                                                                       >  2:1495300/1‑111 (MQ=255)
                                                           aaCCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgtg                                                                                                                       <  1:1495300/111‑1 (MQ=255)
                                                           aaCCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgcg                                                                                                     <  3:334226/129‑1 (MQ=255)
                                                              cATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCctc                                                                              <  2:514799/149‑1 (MQ=255)
                                                               aTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTaa                                                                                                                            <  4:1517108/102‑1 (MQ=255)
                                                                  cTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                          <  3:1018101/149‑1 (MQ=255)
                                                                   tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCctc                                                                              <  1:566938/144‑1 (MQ=255)
                                                                   tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCctc                                                                              >  2:566938/1‑144 (MQ=255)
                                                                   tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                         >  1:1568625/1‑149 (MQ=255)
                                                                     aTTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGtt                                                                                                                              <  4:705167/94‑1 (MQ=255)
                                                                      ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                           <  2:1788347/144‑1 (MQ=255)
                                                                      ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                           >  1:1788347/1‑144 (MQ=255)
                                                                      ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      >  4:407779/1‑149 (MQ=255)
                                                                      ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      >  2:1675798/1‑149 (MQ=255)
                                                                      ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      <  3:936869/149‑1 (MQ=255)
                                                                        gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                    <  3:1653538/149‑1 (MQ=39)
                                                                          aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtgt                                                                                                                               >  2:790895/1‑88 (MQ=255)
                                                                          aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtgt                                                                                                                               <  1:790895/88‑1 (MQ=255)
                                                                          aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                  >  4:1804757/1‑149 (MQ=255)
                                                                           aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      >  1:1532243/1‑144 (MQ=255)
                                                                           aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGGTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      <  2:1532243/144‑1 (MQ=255)
                                                                              ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTcggtcggt                                                                                           >  1:461961/1‑120 (MQ=255)
                                                                              ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                              <  1:1675798/149‑1 (MQ=255)
                                                                              ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                              <  3:1804757/149‑1 (MQ=255)
                                                                               tttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                             <  2:1568625/149‑1 (MQ=255)
                                                                                   cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                         >  3:93580/1‑149 (MQ=255)
                                                                                   cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                         >  3:63502/1‑149 (MQ=255)
                                                                                    tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgc                                                                                                      <  3:1381546/103‑1 (MQ=255)
                                                                                      aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgcgc                                                                                                    <  2:1559292/103‑1 (MQ=255)
                                                                                      aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                          >  4:1140441/1‑129 (MQ=255)
                                                                                      aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                        <  3:983716/147‑1 (MQ=255)
                                                                                      aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                        >  4:983716/1‑147 (MQ=255)
                                                                                         gTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTaa                                                                                                                            >  3:244353/1‑76 (MQ=255)
                                                                                         gTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTaa                                                                                                                            <  4:244353/76‑1 (MQ=255)
                                                                                         gTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                        <  1:1564360/144‑1 (MQ=255)
                                                                                          tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaaa                                                                                                                                   >  4:1331775/1‑68 (MQ=255)
                                                                                          tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaaa                                                                                                                                   <  3:1331775/68‑1 (MQ=255)
                                                                                             cTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                             <  1:1327010/103‑1 (MQ=255)
                                                                                             cTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGgaga                                                                      >  1:18910/1‑126 (MQ=255)
                                                                                              tGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgt                                              <  4:63502/149‑1 (MQ=255)
                                                                                                     cTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCa                                       >  4:1524141/1‑149 (MQ=255)
                                                                                                         gCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                          >  2:82377/1‑110 (MQ=255)
                                                                                                              tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAcc                              >  4:1605754/1‑149 (MQ=255)
                                                                                                               cAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                    <  3:1624499/110‑1 (MQ=255)
                                                                                                               cAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                    >  4:1624499/1‑110 (MQ=255)
                                                                                                                aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACaga                                                                                                                 >  1:1786209/1‑64 (MQ=255)
                                                                                                                aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACaga                                                                                                                 <  2:1786209/64‑1 (MQ=255)
                                                                                                                aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                >  2:236935/1‑113 (MQ=255)
                                                                                                                aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                            >  2:268704/1‑149 (MQ=255)
                                                                                                                  gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                              >  3:1276316/1‑113 (MQ=255)
                                                                                                                  gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                              <  4:1276316/113‑1 (MQ=255)
                                                                                                                   cTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                            <  3:737268/146‑1 (MQ=255)
                                                                                                                   cTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                            >  4:737268/1‑146 (MQ=255)
                                                                                                                   cTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                         <  1:932946/149‑1 (MQ=255)
                                                                                                                     tACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTa                       <  3:1524141/149‑1 (MQ=255)
                                                                                                                         gTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttct                   >  1:1205108/1‑149 (MQ=255)
                                                                                                                          tttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                              >  3:1514690/4‑105 (MQ=25)
                                                                                                                           aGGCCGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                 <  3:1605754/149‑1 (MQ=255)
                                                                                                                               cGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa             >  3:459751/1‑149 (MQ=38)
                                                                                                                               cGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCGCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgt                                              <  3:1610332/116‑1 (MQ=25)
                                                                                                                                 gATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaa           >  3:828301/1‑149 (MQ=255)
                                                                                                                                     aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCaa                                      >  4:1490418/1‑118 (MQ=255)
                                                                                                                                       gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg     >  4:905722/1‑149 (MQ=255)
                                                                                                                                         gTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgt                                              <  1:640692/106‑1 (MQ=255)
                                                                                                                                         gTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgt                                              >  2:640692/1‑106 (MQ=255)
                                                                                                                                         gTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcg   >  2:166053/1‑149 (MQ=255)
                                                                                                                                          ttCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcgc  >  2:1734969/1‑149 (MQ=255)
                                                                                                                                                ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCaaaa                                                                                                          <  2:1285352/39‑1 (MQ=255)
                                                                                                                                                ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                             <  1:813496/52‑1 (MQ=255)
                                                                                                                                                ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                             >  2:813496/1‑52 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGC  >  NC_000913/3908296‑3908582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: