Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,984,227 G→A 5.4% intergenic (+34/+125) aslB → / ← aslA putative AslA‑specific sulfatase‑maturating enzyme/putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,984,2270GA 5.4% 376.8 / 8.2 111intergenic (+34/+125)aslB/aslAputative AslA‑specific sulfatase‑maturating enzyme/putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base G (50/55);  new base A (3/3);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 116 total aligned reads displayed.

TCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGAT  >  NC_000913/3984079‑3984361
                                                                                                                                                    |                                                                                                                                      
tCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATg                                                                                                                                        <  2:987105/149‑1 (MQ=255)
   gCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcgg                                                                                                                                     >  2:1763089/1‑149 (MQ=255)
   gCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcgg                                                                                                                                     <  1:135149/149‑1 (MQ=255)
        aTTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTaa                                                                                                                                >  3:464622/1‑149 (MQ=255)
          ttCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                              <  3:1812/149‑1 (MQ=255)
            ccGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGc                                                                                                                            <  3:1233696/149‑1 (MQ=255)
             cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  4:970223/1‑149 (MQ=255)
             cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  2:149760/1‑149 (MQ=255)
             cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  2:709645/1‑149 (MQ=255)
             cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  3:230236/1‑149 (MQ=255)
              gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcgg                                                                                                                                     >  3:1363566/1‑138 (MQ=255)
              gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCt                                                                                                                          <  1:1763089/149‑1 (MQ=255)
                 aTCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                       >  1:529770/1‑149 (MQ=255)
                   cTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                     <  4:345924/149‑1 (MQ=255)
                     aCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                   <  1:731899/149‑1 (MQ=255)
                     aCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGAGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                   >  2:1772046/1‑149 (MQ=255)
                      ccGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                  >  1:1230288/1‑149 (MQ=255)
                         ccATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                               >  2:1272636/1‑149 (MQ=255)
                          cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                              <  2:529770/149‑1 (MQ=255)
                          cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                              <  1:1081734/149‑1 (MQ=255)
                            tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAt                                                                                                            >  3:1123484/1‑149 (MQ=255)
                            tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAt                                                                                                            <  4:1745298/149‑1 (MQ=255)
                                ttAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTATGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                  >  1:305014/1‑139 (MQ=255)
                                ttAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                  <  2:305014/139‑1 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           <  3:967408/128‑1 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  4:967408/1‑128 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                          >  3:598318/1‑145 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                          >  1:509501/1‑145 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                          <  2:509501/145‑1 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                          <  4:598318/145‑1 (MQ=255)
                                  aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCAGCCTACagaacgct                                                                                                      >  1:322426/1‑142 (MQ=255)
                                   aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                              >  1:1402626/1‑124 (MQ=255)
                                   aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                              <  2:1402626/124‑1 (MQ=255)
                                   aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTg                                                                                                     >  1:812352/1‑149 (MQ=255)
                                           gCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCtt                                                                                                                         >  2:256906/1‑121 (MQ=255)
                                           gCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCa                                                                                             >  4:238523/1‑149 (MQ=255)
                                            cTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            <  3:394/149‑1 (MQ=255)
                                            cTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            >  4:394/1‑149 (MQ=255)
                                             tGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                 >  2:1181994/1‑143 (MQ=255)
                                             tGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                 <  1:1181994/143‑1 (MQ=255)
                                               aTTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                 <  4:1834479/141‑1 (MQ=255)
                                               aTTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCCGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGACCGTGCaaa                                                                                                 >  3:1834479/1‑141 (MQ=255)
                                                     tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                 >  1:948799/1‑135 (MQ=255)
                                                     tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                 <  2:948799/135‑1 (MQ=255)
                                                     tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                                                                   <  4:464622/149‑1 (MQ=255)
                                                       gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            <  2:1402827/138‑1 (MQ=255)
                                                       gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            >  1:1402827/1‑138 (MQ=255)
                                                       gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                 <  4:1123484/149‑1 (MQ=255)
                                                         gcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAACTCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           >  3:1858999/1‑105 (MQ=255)
                                                         gcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                           <  4:1858999/105‑1 (MQ=255)
                                                               tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            <  1:1361099/130‑1 (MQ=255)
                                                               tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                            >  2:1361099/1‑130 (MQ=255)
                                                               tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAAtt                                                                                      >  4:1479255/1‑136 (MQ=255)
                                                               tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                                                                   >  2:1145681/1‑139 (MQ=255)
                                                               tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                                                                   <  1:1145681/139‑1 (MQ=255)
                                                                         aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                                                    >  1:413316/1‑112 (MQ=255)
                                                                         aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                                                    <  2:413316/112‑1 (MQ=255)
                                                                         aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATaa                                                               >  1:1162236/1‑149 (MQ=255)
                                                                           cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGa                                                             <  3:970223/149‑1 (MQ=255)
                                                                           cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGa                                                             <  2:1162236/149‑1 (MQ=255)
                                                                                ttATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATg                                                           <  3:454677/146‑1 (MQ=255)
                                                                                ttATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGt                                                        <  2:1230288/149‑1 (MQ=255)
                                                                                   tgcatgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                     >  1:1355898/1‑133 (MQ=255)
                                                                                    gcatgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgc                                                    <  1:709645/149‑1 (MQ=255)
                                                                                      atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCTGGCCTACATGATCGTGCAAATTCAATaaa                                                                                        >  4:379931/1‑111 (MQ=255)
                                                                                      atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATaaa                                                                                        <  2:675674/111‑1 (MQ=255)
                                                                                      atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATaaa                                                                                        >  1:675674/1‑111 (MQ=255)
                                                                                      atgcGCAGTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCTGGCCTACATGATCGTGCAAATTCAATaaa                                                                                        <  3:379931/111‑1 (MQ=255)
                                                                                         cgcATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatcgc                                               <  1:1272636/149‑1 (MQ=255)
                                                                                            aTTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATc                                                                                                        >  3:863747/1‑89 (MQ=255)
                                                                                            aTTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATc                                                                                                        <  4:863747/89‑1 (MQ=255)
                                                                                             tttGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAgg                                                                       <  1:1633140/121‑1 (MQ=255)
                                                                                                  tggtggTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTc                                                                                              <  2:1548746/93‑1 (MQ=255)
                                                                                                  tggtggTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTc                                                                                              >  1:1548746/1‑93 (MQ=255)
                                                                                                   ggtggtGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCaaa                                     <  1:1772046/149‑1 (MQ=255)
                                                                                                    gtggtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                     >  1:1049258/1‑116 (MQ=255)
                                                                                                    gtggtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                     <  2:1049258/116‑1 (MQ=255)
                                                                                                       gtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                 <  4:1037866/69‑1 (MQ=255)
                                                                                                       gtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                 >  3:1037866/1‑69 (MQ=255)
                                                                                                        tgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATAAGGCGTTTACGCCGCATCCGGca                                                                                                                 <  4:58395/68‑2 (MQ=17)
                                                                                                            taagtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcat                                                  <  3:251896/127‑1 (MQ=255)
                                                                                                            taagtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcat                                                  >  4:251896/1‑127 (MQ=255)
                                                                                                               gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                 <  2:751795/93‑1 (MQ=255)
                                                                                                               gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                 >  1:751795/1‑93 (MQ=255)
                                                                                                               gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                 >  3:990523/1‑125 (MQ=255)
                                                                                                                        ggcggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTgg                                                                   >  3:1711602/1‑98 (MQ=255)
                                                                                                                        ggcggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTgg                                                                   <  4:1711602/98‑1 (MQ=255)
                                                                                                                           ggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCgg             >  3:408340/1‑149 (MQ=255)
                                                                                                                                 cTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGt       <  2:656601/149‑1 (MQ=255)
                                                                                                                                      gTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGAt  <  4:408340/149‑1 (MQ=255)
                                                                                                                                       ttGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                 <  2:1765088/101‑1 (MQ=255)
                                                                                                                                       ttGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                 >  1:1765088/1‑101 (MQ=255)
                                                                                                                                           ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCg              <  1:1380684/132‑1 (MQ=255)
                                                                                                                                           ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCg              >  2:1380684/1‑132 (MQ=255)
                                                                                                                                             gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTACAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGa                                                                  <  1:178280/78‑1 (MQ=255)
                                                                                                                                             gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTACAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGa                                                                  >  2:178280/1‑78 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                              >  2:1349277/1‑114 (MQ=37)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                              >  4:1889741/1‑114 (MQ=37)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                              <  3:1889741/114‑1 (MQ=37)
                                                                                                                                                 gATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGt                                                                                                      <  1:400048/38‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
TCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGAT  >  NC_000913/3984079‑3984361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: