Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,984,234 A→T 5.1% intergenic (+41/+118) aslB → / ← aslA putative AslA‑specific sulfatase‑maturating enzyme/putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,984,2340AT 5.1% 422.9 / 9.8 117intergenic (+41/+118)aslB/aslAputative AslA‑specific sulfatase‑maturating enzyme/putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (54/57);  new base T (3/3);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 118 total aligned reads displayed.

ATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTG  >  NC_000913/3984087‑3984380
                                                                                                                                                   |                                                                                                                                                  
aTTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTaa                                                                                                                                                   >  3:464622/1‑149 (MQ=255)
  ttCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                                                 <  3:1812/149‑1 (MQ=255)
    ccGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGc                                                                                                                                               <  3:1233696/149‑1 (MQ=255)
     cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  4:970223/1‑149 (MQ=255)
     cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  2:1081734/1‑149 (MQ=255)
     cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  2:149760/1‑149 (MQ=255)
     cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  2:709645/1‑149 (MQ=255)
     cGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  3:230236/1‑149 (MQ=255)
      gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCt                                                                                                                                             <  1:1763089/149‑1 (MQ=255)
         aTCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                          >  1:529770/1‑149 (MQ=255)
           cTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                        <  4:345924/149‑1 (MQ=255)
             aCCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGAGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                      >  2:1772046/1‑149 (MQ=255)
              ccGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     >  1:1230288/1‑149 (MQ=255)
                 ccATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                                  >  2:1272636/1‑149 (MQ=255)
                  cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                 <  4:230236/149‑1 (MQ=255)
                  cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                 <  2:529770/149‑1 (MQ=255)
                  cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                 <  1:1081734/149‑1 (MQ=255)
                  cATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                                 <  1:149760/149‑1 (MQ=255)
                    tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAt                                                                                                                               >  3:1123484/1‑149 (MQ=255)
                        ttAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTATGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     >  1:305014/1‑139 (MQ=255)
                        ttAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                     <  2:305014/139‑1 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  4:967408/1‑128 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              <  3:967408/128‑1 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                                             <  4:598318/145‑1 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                                             >  3:598318/1‑145 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                                                                             <  2:509501/145‑1 (MQ=255)
                          aaaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCAGCCTACagaacgct                                                                                                                         >  1:322426/1‑142 (MQ=255)
                           aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                                                 <  2:1402626/124‑1 (MQ=255)
                           aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAAc                                                                                                                                                 >  1:1402626/1‑124 (MQ=255)
                           aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTg                                                                                                                        >  1:812352/1‑149 (MQ=255)
                                   gCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCtt                                                                                                                                            <  1:256906/121‑1 (MQ=255)
                                   gCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCtt                                                                                                                                            >  2:256906/1‑121 (MQ=255)
                                   gCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCa                                                                                                                >  1:656601/1‑149 (MQ=255)
                                    cTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               <  3:394/149‑1 (MQ=255)
                                    cTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               >  4:394/1‑149 (MQ=255)
                                     tGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                                    <  1:1181994/143‑1 (MQ=255)
                                     tGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                                    >  2:1181994/1‑143 (MQ=255)
                                       aTTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                                    <  4:1834479/141‑1 (MQ=255)
                                       aTTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCCGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGACCGTGCaaa                                                                                                                    >  3:1834479/1‑141 (MQ=255)
                                             tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                                    <  2:948799/135‑1 (MQ=255)
                                             tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCaaa                                                                                                                    >  1:948799/1‑135 (MQ=255)
                                               gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               <  2:1402827/138‑1 (MQ=255)
                                               gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               >  1:1402827/1‑138 (MQ=255)
                                               gcgcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                                    <  4:1123484/149‑1 (MQ=255)
                                                 gcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAACTCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              >  3:1858999/1‑105 (MQ=255)
                                                 gcACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGcc                                                                                                                                              <  4:1858999/105‑1 (MQ=255)
                                                       tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               >  2:1361099/1‑130 (MQ=255)
                                                       tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCaa                                                                                                               <  1:1361099/130‑1 (MQ=255)
                                                       tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAAtt                                                                                                         >  4:1479255/1‑136 (MQ=255)
                                                       tCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                                                                                      <  1:1145681/139‑1 (MQ=255)
                                                                 aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                                                                       <  2:413316/112‑1 (MQ=255)
                                                                 aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                                                                       >  1:413316/1‑112 (MQ=255)
                                                                 aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATaa                                                                                  >  1:1162236/1‑149 (MQ=255)
                                                                   cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGa                                                                                <  2:1162236/149‑1 (MQ=255)
                                                                   cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCCGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGa                                                                                <  2:812352/149‑1 (MQ=255)
                                                                    gACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGAt                                                                               <  3:238523/149‑1 (MQ=255)
                                                                        ttATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATg                                                                              >  4:454677/1‑146 (MQ=255)
                                                                        ttATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGt                                                                           <  2:1230288/149‑1 (MQ=255)
                                                                           tgcatgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                                        >  1:1355898/1‑133 (MQ=255)
                                                                            gcatgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgc                                                                       <  1:709645/149‑1 (MQ=255)
                                                                              atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCTGGCCTACATGATCGTGCAAATTCAATaaa                                                                                                           >  4:379931/1‑111 (MQ=255)
                                                                              atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATaaa                                                                                                           >  1:675674/1‑111 (MQ=255)
                                                                              atgcGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATaaa                                                                                                           <  2:675674/111‑1 (MQ=255)
                                                                              atgcGCAGTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCTGGCCTACATGATCGTGCAAATTCAATaaa                                                                                                           <  3:379931/111‑1 (MQ=255)
                                                                                    aTTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATc                                                                                                                           <  4:863747/89‑1 (MQ=255)
                                                                                    aTTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATc                                                                                                                           >  3:863747/1‑89 (MQ=255)
                                                                                     tttGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAgg                                                                                          <  1:1633140/121‑1 (MQ=255)
                                                                                          tggtggTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTc                                                                                                                 <  2:1548746/93‑1 (MQ=255)
                                                                                          tggtggTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTc                                                                                                                 >  1:1548746/1‑93 (MQ=255)
                                                                                            gtggtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                                        >  1:1049258/1‑116 (MQ=255)
                                                                                            gtggtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCt                                                                                        <  2:1049258/116‑1 (MQ=255)
                                                                                               gtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                    <  4:1037866/69‑1 (MQ=255)
                                                                                               gtgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                    >  3:1037866/1‑69 (MQ=255)
                                                                                                tgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATAAGGCGTTTACGCCGCATCCGGca                                                                                                                                    <  4:58395/68‑2 (MQ=17)
                                                                                                tgAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATAAGGCGTTTACGCCGCATCCGGca                                                                                                                                    >  3:58395/1‑67 (MQ=17)
                                                                                                    taagtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcat                                                                     >  4:251896/1‑127 (MQ=255)
                                                                                                    taagtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcat                                                                     <  3:251896/127‑1 (MQ=255)
                                                                                                       gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                                    >  1:751795/1‑93 (MQ=255)
                                                                                                       gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                                                                                    <  2:751795/93‑1 (MQ=255)
                                                                                                       gtaGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                                    >  3:990523/1‑125 (MQ=255)
                                                                                                                ggcggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTgg                                                                                      <  4:1711602/98‑1 (MQ=255)
                                                                                                                ggcggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTgg                                                                                      >  3:1711602/1‑98 (MQ=255)
                                                                                                                   ggcCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCgg                                >  3:408340/1‑149 (MQ=255)
                                                                                                                         cTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGt                          <  2:656601/149‑1 (MQ=255)
                                                                                                                              gTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGAt                     <  4:408340/149‑1 (MQ=255)
                                                                                                                               ttGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                                    <  2:1765088/101‑1 (MQ=255)
                                                                                                                               ttGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatc                                                                    >  1:1765088/1‑101 (MQ=255)
                                                                                                                                   ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCg                                 <  1:1380684/132‑1 (MQ=255)
                                                                                                                                   ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCg                                 >  2:1380684/1‑132 (MQ=255)
                                                                                                                                     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTACAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGa                                                                                     <  1:178280/78‑1 (MQ=255)
                                                                                                                                     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTACAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGa                                                                                     >  2:178280/1‑78 (MQ=255)
                                                                                                                                     gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                                                 >  4:1889741/1‑114 (MQ=37)
                                                                                                                                     gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                                                 <  1:1349277/114‑1 (MQ=37)
                                                                                                                                     gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGAt                                                 >  2:1349277/1‑114 (MQ=37)
                                                                                                                                         gATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGt                                                                                                                         <  1:400048/38‑1 (MQ=255)
                                                                                                                                              ggcgTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCgtg     >  4:217999/1‑149 (MQ=255)
                                                                                                                                                 gTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCgtggtg  >  2:1028375/1‑149 (MQ=255)
                                                                                                                                                  tAAACGCCTTATCCGGCCTACATGATCGTGCAAAtt                                                                                                                  >  4:160379/1‑36 (MQ=255)
                                                                                                                                                  tAAACGCCTTATCCGGCCTACATGATCGTGCAAAtt                                                                                                                  <  3:160379/36‑1 (MQ=255)
                                                                                                                                                  tAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCa                                                    >  1:711555/1‑98 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTG  >  NC_000913/3984087‑3984380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: