Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,339,818 Δ1 bp 7.1% coding (248/810 nt) mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,339,8180A. 7.1% 394.8 / 21.8 113coding (248/810 nt)mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (60/45);  new base . (3/5);  total (63/50)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

Only 100 of 116 total aligned reads displayed.

CCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAT  >  NC_000913/3339545‑3340090
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
ccAACAAAAGGTTCATCAAACATGATGAGATCCGGCACCAGCGCAAACGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCa                                                                                                                                                                                                                                                                                   <  2:107639/274‑1 (MQ=255)
              atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATCATCATCCGGTTGCGCACTGTATACactgtc                                                                                                                                                                                                                                                                     >  2:487772/1‑269 (MQ=255)
                               ccGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGCAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGa                                                                                                                                                                                                                                                  >  1:114446/1‑275 (MQ=255)
                                                   tgggatggcgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               >  2:300891/8‑274 (MQ=255)
                                                        cAGCGCTGCCAGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtct                                                                                                                                                                                                                         <  2:460119/275‑1 (MQ=255)
                                                            gcTGCACGCCGCGCCATCCAACCGGAAAGTTCCAAAGGCATCAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTATGATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc                                                                                                                                                                                                                     <  4:300887/275‑1 (MQ=255)
                                                                                           cAGAAGGCATTATTTTAGCCGCACCCAGNGNGCCCACGGCCTCAAGCTTCATCATCCCCGTACTATGCAACAAGGGNGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACNCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGa                                                                                                                                                                                      <  3:42799/275‑1 (MQ=255)
                                                                                                              cgacgcACGGAGCCCCACGCACCCTAGCTNNAACATCAACCGACGATGCAACAATGGCGCGNAGAGTTGGGTATGTTAGCGCAGTGGATAGGCGACGTTGTAAAATACGTTCATATCATTGAACAACGACCCGGACNGAAATAACCTGCTCATCCGGTTGCGCACTGTAGACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAc                                                                                                                                                                   <  3:60647/270‑1 (MQ=255)
                                                                                                                cTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGCTCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAcc                                                                                                                                                                  >  2:12481/1‑274 (MQ=255)
                                                                                                                                 gCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGt                                                                                                                                                                                                                                                                               <  5:16657/149‑1 (MQ=255)
                                                                                                                                        gCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc                                                                                                                                                                                             <  2:346721/223‑1 (MQ=255)
                                                                                                                                          ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgcg                                                                                                                                                                                                                                                                     >  7:416028/1‑149 (MQ=255)
                                                                                                                                          ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgcg                                                                                                                                                                                                                                                                     >  5:232479/1‑149 (MQ=255)
                                                                                                                                             atcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata                                                                                                                                                                                                                                                                            >  8:231010/1‑140 (MQ=255)
                                                                                                                                             atcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata                                                                                                                                                                                                                                                                            <  7:231010/140‑1 (MQ=255)
                                                                                                                                              tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTGATCCGTTTGCGCACTGTATACAGGCGCGaa                                                                                                                                                                                                                                                                 >  5:309038/1‑149 (MQ=255)
                                                                                                                                              tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGaa                                                                                                                                                                                                                                                                 >  7:286783/1‑149 (MQ=255)
                                                                                                                                               catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAAc                                                                                                                                                                                                                                                                <  8:125995/149‑1 (MQ=255)
                                                                                                                                                atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACg                                                                                                                                                                                                                                                               >  8:212454/1‑149 (MQ=255)
                                                                                                                                                atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACg                                                                                                                                                                                                                                                               >  8:332774/1‑149 (MQ=255)
                                                                                                                                                 tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACgg                                                                                                                                                                                                                                                              <  5:34897/149‑2 (MQ=255)
                                                                                                                                                   aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACgaga                                                                                                                                                                                                                                                            <  6:289526/149‑1 (MQ=255)
                                                                                                                                                    ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt                                                                                                                             <  2:129950/275‑1 (MQ=255)
                                                                                                                                                    ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACggat                                                                                                                                                                                                                                                            <  5:72679/148‑4 (MQ=255)
                                                                                                                                                    ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACggat                                                                                                                                                                                                                                                            >  6:72679/1‑145 (MQ=255)
                                                                                                                                                            tGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACGCCGTGACCTTCCCTCGCGGCAc                                                                                                                     >  2:510805/1‑275 (MQ=255)
                                                                                                                                                               aacaacGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAGAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGt                                                                                                                  <  2:100247/275‑1 (MQ=255)
                                                                                                                                                               aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  6:186641/1‑149 (MQ=255)
                                                                                                                                                               aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  5:135469/1‑149 (MQ=255)
                                                                                                                                                               aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  8:50186/1‑149 (MQ=255)
                                                                                                                                                                 caaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACCCCGTAATCTTCCCTcg                                                                                                                           >  1:44597/1‑264 (MQ=255)
                                                                                                                                                                     ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtctc                                                                                                                                                                                                                                          <  8:416028/149‑1 (MQ=255)
                                                                                                                                                                      gcgcGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCa                                                                                                                                                                                                                                         <  8:372736/149‑1 (MQ=255)
                                                                                                                                                                               gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCCTGtat                                                                                                                                                                                                                                                                             <  5:272493/104‑1 (MQ=255)
                                                                                                                                                                               gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCCTGtat                                                                                                                                                                                                                                                                             >  6:272493/1‑104 (MQ=255)
                                                                                                                                                                                    gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt                                                                                                                                                                                                                           >  8:60195/1‑149 (MQ=255)
                                                                                                                                                                                        gTTCGCGCAGTGGATAGTCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCNGAATATTNCCANCATCGANAAGGAt                                                                                                                                                                                                                           >  3:24497/1‑145 (MQ=255)
                                                                                                                                                                                            gcgcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa                                                                                                                                                                                                                       <  1:24497/145‑1 (MQ=255)
                                                                                                                                                                                                aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATc                                                                                                                                                                                                               <  7:332774/149‑1 (MQ=255)
                                                                                                                                                                                                 gTGGATAGGCGACTTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAAGAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGcc                                                                                 <  2:395219/274‑1 (MQ=255)
                                                                                                                                                                                                   ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg                                                                                                                                                                                                            >  5:348281/1‑149 (MQ=255)
                                                                                                                                                                                                   ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGcc                                                                                                                                                            >  4:162151/1‑197 (MQ=255)
                                                                                                                                                                                                     aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg                                                                                                                                                                                                          <  5:186641/149‑1 (MQ=255)
                                                                                                                                                                                                       aGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt                                                                                                                                                        <  2:162153/197‑1 (MQ=255)
                                                                                                                                                                                                        ggCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGa                                                                                                                                                                                                       <  7:60195/149‑1 (MQ=255)
                                                                                                                                                                                                         gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                                                                                                                                                      >  7:33318/1‑149 (MQ=255)
                                                                                                                                                                                                         gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                                                                                                                                                      <  6:56557/149‑1 (MQ=255)
                                                                                                                                                                                                         gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                                                                                                                                                      >  6:72181/1‑149 (MQ=255)
                                                                                                                                                                                                          cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgcg                                                                                                                                                                                                                                                                     >  3:76239/1‑85 (MQ=255)
                                                                                                                                                                                                          cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                                                                                                                                                     <  7:50186/149‑1 (MQ=255)
                                                                                                                                                                                                           gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            >  5:30196/1‑109 (MQ=255)
                                                                                                                                                                                                           gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            <  6:30196/109‑1 (MQ=255)
                                                                                                                                                                                                              gTTGTCAAATACGTTCATATCATTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACg                                                                                                                                                                                                                                                               <  7:143844/87‑1 (MQ=25)
                                                                                                                                                                                                              gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGaa                                                                                                                                                                                                                                                                 <  1:76239/85‑1 (MQ=255)
                                                                                                                                                                                                              gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAAGATTAGCCGAGAAGACACGATTGCCCCGCGTaaaccgt                                                                    >  4:332175/1‑270 (MQ=255)
                                                                                                                                                                                                              gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACg                                                                                                                                                                                                                                                               >  8:143844/1‑87 (MQ=37)
                                                                                                                                                                                                                tGTCAAATACGTTCATATCAGTGAACAACGCCCAGGACTGAAAGAACATGCGCATCCTTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt                                                                                                                             <  2:322815/215‑1 (MQ=255)
                                                                                                                                                                                                                 gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc                                                                                                                                                                                              <  7:212454/149‑1 (MQ=255)
                                                                                                                                                                                                                  tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATCACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCCGCACGGTCAGGGAACTATTATCGACGATGCCGCGATTGCCACGTGTaaaaattaaatc                                                               >  1:39390/1‑267 (MQ=255)
                                                                                                                                                                                                                  tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACga                                                                                                                                                                                                                                                              <  8:80836/84‑1 (MQ=255)
                                                                                                                                                                                                                    aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt                                                                                                                                                                                                           <  5:402135/133‑1 (MQ=255)
                                                                                                                                                                                                                    aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCaa                                                                                                                                                                                           >  5:456740/1‑149 (MQ=255)
                                                                                                                                                                                                                      aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATc                                                                                                                                                                                         >  5:158925/1‑149 (MQ=255)
                                                                                                                                                                                                                       tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg                                                                                                                                                                                                                              >  7:379309/1‑111 (MQ=255)
                                                                                                                                                                                                                          gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCCCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCCGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACCTcgccgata                                                        >  2:194509/1‑269 (MQ=255)
                                                                                                                                                                                                                           ttCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACg                                                                                                                                                                                    >  8:300465/1‑149 (MQ=255)
                                                                                                                                                                                                                            tCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACTCGTAAAACTGACATCGCGCATATCg                                                     >  2:273296/1‑275 (MQ=255)
                                                                                                                                                                                                                                atCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTa                                                                                                                                                                               >  8:108497/1‑149 (MQ=255)
                                                                                                                                                                                                                                 tCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAg                                                                                                                                                                              >  8:267472/1‑149 (MQ=255)
                                                                                                                                                                                                                                   cgataacaacGCCCCAGACTGAAATAACATGCTCATCCGTTTGCGCACGGTATACAGGCGCTAACGGGACATCGCCGGAATATTCTCACCATCGAAAAGGTGCTCACCAGGATCTGGTGCGATTGGCCCGCCAAGCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAAt                                              <  4:273292/271‑1 (MQ=255)
                                                                                                                                                                                                                                   aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg                                                                                                                                                                            >  6:429885/1‑149 (MQ=255)
                                                                                                                                                                                                                                    gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt                                                                                                                                                                           >  8:273549/1‑149 (MQ=255)
                                                                                                                                                                                                                                     tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc                                                                                                                                                                          >  7:17042/1‑149 (MQ=255)
                                                                                                                                                                                                                                       aacaacGCCCCGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt                                                                                                                                                                        >  8:125793/1‑149 (MQ=255)
                                                                                                                                                                                                                                          aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtct                                                                                                                                                                                                                         >  3:14813/1‑97 (MQ=255)
                                                                                                                                                                                                                                          aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCa                                                                                                                                                 >  1:83597/1‑169 (MQ=255)
                                                                                                                                                                                                                                           aagCCCCGGACTGAAAAAACCATTGCATCCGTTTGCGAAAGGGAAACAGGCGCGAACGAGACAGCGCCGGAATATTCTCACCATCGAAAAGGGTCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTGACCGATGCCCGATGGACCCATGATCGCCGTGATCTTCCCTCGCGGCACTGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCcaca                                      <  4:194507/273‑1 (MQ=255)
                                                                                                                                                                                                                                            cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCCAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATAACGACTAAATTCGccccc                                      >  2:9835/1‑271 (MQ=255)
                                                                                                                                                                                                                                              ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc                                                                                                                                                                                                                     <  1:14813/97‑1 (MQ=255)
                                                                                                                                                                                                                                              ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAt                                                                                                                                             <  3:83597/169‑1 (MQ=255)
                                                                                                                                                                                                                                               cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               <  6:380537/86‑1 (MQ=255)
                                                                                                                                                                                                                                               cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               >  5:380537/1‑86 (MQ=255)
                                                                                                                                                                                                                                                  caaCTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGTCCCGCACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGACGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCt                               <  2:514111/273‑1 (MQ=255)
                                                                                                                                                                                                                                                     cTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt                                                                                                                                                                                                                           <  3:78891/84‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc                                                                                                                                                             <  5:453857/145‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc                                                                                                                                                             >  6:453857/1‑145 (MQ=255)
                                                                                                                                                                                                                                                      tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc                                                                                                                                                             <  7:229791/145‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGa                                                                                                                                                         >  8:430013/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        aaaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            >  5:51822/1‑64 (MQ=255)
                                                                                                                                                                                                                                                        aaaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            <  6:51822/64‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa                                                                                                                                                                                  >  6:281077/1‑122 (MQ=255)
                                                                                                                                                                                                                                                          aTAACATGCTCCTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCTGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAAtt                       <  1:56522/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaCATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGccc                                                                                                                                                   >  6:265220/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  cTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAAGGTCAGGGAAATCTTATCGAAGATGCAGCGATTGCCAAGCGTAAAACTGACATCGCGATTATAGAATCAATTCTCCACACCCTGCCACACAATTCACCATTc               >  4:274836/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                     aTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg                                                                                                                                                                                                   <  8:61186/92‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg                                                                                                                                                                                                   >  7:61186/1‑92 (MQ=255)
                                                                                                                                                                                                                                                                     aTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc                                                                                                                                          >  5:307493/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         gTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg                                                                                                                                      <  5:429885/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ttGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAt                                                                                                                                    <  7:84713/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gcACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGGCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTAAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAt  <  2:53217/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
CCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAT  >  NC_000913/3339545‑3340090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: