Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 151,519 | A→C | 5.1% | I27M (ATT→ATG) | yadK ← | putative fimbrial‑like adhesin protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 151,519 | 0 | A | C | 5.1% | 406.1 / 11.5 | 118 | I27M (ATT→ATG) | yadK | putative fimbrial‑like adhesin protein |
Reads supporting (aligned to +/- strand): ref base A (56/56); new base C (3/3); total (59/59) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01 |
Only 100 of 120 total aligned reads displayed.
AGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGC > NC_000913/151263‑151778 | agCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATACCTTTCAGGAGTAACCTTACCTGAAAAATATCCCAACCCAACGACGCCAAGATAAATATTACCCTGGTTACTGATGCCGCTTTTGCAGGTGCTGTTTTTTACTTTGGCAACAAGTTCAATATCCTGTCCAGCAATGCC‑TGGACAGGCAAta > 1:101482/1‑274 (MQ=255) gtataagagacatccGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCaaa < 1:18235/262‑1 (MQ=255) gcagcCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCaaaa > 1:144647/1‑275 (MQ=255) tctAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGCTAACTTTCAGGAGTAACATTACCTGAAAAATATCACACCCCAACGACGCCAAGATCCATATTACCCTGGTTACTGCTTCCGCTTTTACAGGTGCTGTTTTTTACATTATCAACAAGGTCAATATCCTGTCCAGCAATGGCGCGGAC‑GGGGATGCAAAACAGGAATTAAAGAAACAGAgtt > 4:17856/1‑274 (MQ=255) tctAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTTATTCCGCTTTTGCCGGTGCTGTTTTTTACATTTGAAACAAGGTCAATTTCCTTTCCCGCAATGCC‑TGGACAGGTGGNGCCAANNAATAATAaccattttctttta > 3:18235/1‑262 (MQ=255) aaattttaacctaTTTAGCTAATCGCCATNNCCCTTTATTGCACAGTCGCATTCATTGATANTCACCTCTTTTCCACCTGGTTAATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGTCGCCANGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGGTGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca < 3:68943/263‑1 (MQ=255) cGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGCATTATTCCCTTCAACAGCTTACTTTGAGGGGGATGc > 4:490575/1‑275 (MQ=255) tAATGCAAAGTCGGTTAACTTTATAGTGANNNCTTTTCCACCTGGTTCAAAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCCAAGACGCCAAGATAAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCAGTCCATCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACTTTGAGTGGGATGCATATATAGTTCCt < 3:144647/275‑1 (MQ=255) aGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCACTATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAGGGTCAATATCCTGTCCAGCAATGGC‑TGTACAGGCGTTGCAAAACAGTAATGACACACAAAGAATTATCCTCTTCATAAGCTTACGTTGTGTGGGCTACATATCTTGTTCTTGActcttt > 4:72181/1‑273 (MQ=255) aGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAAAAGAATTATTCTCTTCATCAGCTTACGTTGAGt > 2:136155/1‑247 (MQ=255) tACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCACTGGC‑TGGACAGGCGATGCAAAACAGTACTGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGGGTGGGATGCCTATATAGTTCCAGAATCTTTAAGTCTg > 4:446902/1‑275 (MQ=255) tACAGTGATAGTGAACTCGTATCCACCTGGTTGATAACGTTCAGGAGTAACATTACCTGAAAAATATCCCAGCCCAACGAAGCCAAGATCAATATTACCCGGGTTACTGATTCCTCTTTTACAGGTGCTGTTATTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGAGTCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTg < 1:40814/275‑1 (MQ=255) aCAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgt < 2:108294/275‑1 (MQ=255) cAGGGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtt < 2:17856/275‑1 (MQ=255) tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGTCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGaca > 2:518258/1‑198 (MQ=255) tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt > 2:225491/1‑235 (MQ=255) tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTCTTGCAGGTGCTGTTCTTTACATTGGNAACAAGGCCAATATCCTGTTCCGCAATGGC‑TGGACAGGCGATGCAAAACCGTAATGAAAGAAACAGAAAAATACGCTCAANCAGACTACCATGAGTGGGAGGCAAANAAAGTTCTCGAATCTGGAAGtaggtgtgt > 3:84330/1‑267 (MQ=255) aTAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc > 4:153726/1‑213 (MQ=255) tAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtat > 2:255631/1‑275 (MQ=255) aGTNAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTANCATTNCCTGAAAAATAGCCCACCCCAACGACGTCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGacagaaa < 4:518253/198‑1 (MQ=255) aGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttagtta < 1:84330/273‑1 (MQ=255) ctctTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTATGTTAGTTATAGGTTAc > 2:68705/1‑275 (MQ=255) tCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCaa < 7:4659/149‑1 (MQ=255) ccACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAAt > 7:308217/1‑149 (MQ=255) ccTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGacagaaacaga > 4:81586/1‑186 (MQ=255) tGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑Tg < 5:333711/149‑1 (MQ=255) tGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑Tg < 5:398645/149‑1 (MQ=255) ggTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTTCTGTTTTTTACATCGGCAACACGGTCAATATCCTGTCCAGCAATGGC‑TTGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt > 2:403449/1‑212 (MQ=255) ggTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGc < 2:490580/274‑1 (MQ=255) gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGa < 6:68080/149‑1 (MQ=255) gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatta < 2:81587/186‑1 (MQ=255) gcaagctgtCAGGCGTAACATTACCTGAACAAAACCCCACCCCAACGACGCCAAGATCACTATTACCCTGGTTACTGATTCCGTTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTACAGAAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTg < 4:85752/267‑1 (MQ=255) gATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAg > 6:66667/1‑149 (MQ=255) aCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGa < 5:66667/149‑1 (MQ=255) tttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATg > 8:454290/1‑149 (MQ=255) ttCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaa > 2:70206/1‑274 (MQ=255) ggAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCaaaa > 6:52076/1‑149 (MQ=255) aaCATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGa > 4:327820/1‑130 (MQ=255) aaCATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCt > 2:85753/1‑255 (MQ=255) ttACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAgg < 2:327824/130‑1 (MQ=255) tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGacagaa > 6:103965/1‑149 (MQ=255) gAAAAATATCCCACCCCAACGAAGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACAATGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGacagaaa < 5:275425/149‑1 (MQ=255) aaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCaaaa > 8:213485/1‑130 (MQ=255) aaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCaaaa < 7:213485/130‑1 (MQ=255) atatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGacagaaacagaa > 6:336844/1‑149 (MQ=255) tCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatta < 6:266755/149‑1 (MQ=255) cccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCACTGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattat > 8:243942/1‑149 (MQ=255) ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt < 5:52076/149‑1 (MQ=255) cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTa > 5:330774/1‑149 (MQ=255) aTCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt < 5:455460/149‑1 (MQ=255) tCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTg > 8:148413/1‑149 (MQ=255) tatTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa > 4:529703/1‑146 (MQ=255) tttccccTGGTTACTTATTCCGCTTTTGCAGGGTCTTTTATTTACATGGGCAACAAGGTCAAAATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGGAATGACAGAACCAGAATTATTCTCTTCATCAGCTTACTGTGAGTGGGATGCATATATAGTTACTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTg < 4:78314/270‑1 (MQ=255) atTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg > 6:365767/1‑149 (MQ=255) ttACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTggg > 7:340389/1‑149 (MQ=255) ttACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTggg > 5:162021/1‑149 (MQ=255) tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa < 8:308217/149‑1 (MQ=255) aCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg < 2:529708/146‑1 (MQ=255) gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCa > 4:100541/1‑146 (MQ=255) cTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGTGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatata < 2:100542/146‑1 (MQ=255) gATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATAGATAGTTCCTGAATCTGTAAGTCAGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACCCATCGGataat > 2:466700/1‑274 (MQ=255) tCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCt < 6:330774/149‑1 (MQ=255) ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATc > 5:97472/1‑149 (MQ=255) tGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGt < 7:454290/149‑1 (MQ=255) tcAGGGGGTGGTTTTTACATTGGAAAAAAGGGCAATATACTGTCAAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt < 4:70205/274‑1 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa < 7:148413/149‑1 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa < 5:103965/149‑1 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgt > 8:122584/1‑149 (MQ=255) tGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCt < 5:109371/146‑1 (MQ=255) tGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCt > 6:109371/1‑146 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTTAATCTGTAAGTCTgttag > 8:286528/1‑149 (MQ=255) ttgttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTgg < 2:446907/272‑1 (MQ=255) tACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtata < 7:56571/149‑1 (MQ=255) aCATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAg < 8:340389/149‑1 (MQ=255) cATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt < 8:234849/103‑1 (MQ=255) cATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt > 7:234849/1‑103 (MQ=255) tGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAg > 2:523853/1‑131 (MQ=255) ggCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtata < 6:49453/143‑1 (MQ=255) ggCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTtata > 5:49453/1‑143 (MQ=255) gCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACATAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACt > 8:33343/1‑149 (MQ=255) aacaaGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCg < 2:85779/178‑1 (MQ=255) aaGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAATTATAGGTTACTGTaaa > 5:52355/1‑149 (MQ=255) aGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAt > 8:201257/1‑149 (MQ=255) gTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAtgt > 6:259473/1‑149 (MQ=255) taaaTATCCTGTCCAGCAATGGC‑TGGAAAGGCAATGAAAAAAAGTAATGCCAGAAACAGAATTATTCCCTTAATCAGCTTACTTGGAGTGGGAGGCATATATATTTCCTGAATTTGTAAGTCTG‑TAGTTATAGGTTACTGTAAATGTgg < 7:286528/147‑1 (MQ=255) atcgccTGTCCAGCCATTGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa < 7:149056/86‑1 (MQ=38) atcgccTGTCCAGCCATTGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa > 8:149056/5‑90 (MQ=38) ccTGTCCAGCCATTGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt > 7:82928/1‑99 (MQ=255) cTGTCCAGCCATTGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGa > 6:78178/1‑103 (MQ=255) tGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa > 1:61639/1‑78 (MQ=255) gTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGACTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTa < 6:388674/108‑1 (MQ=255) gTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTc > 2:369301/1‑97 (MQ=255) gTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTa > 5:388674/1‑108 (MQ=255) aGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGa < 4:369296/97‑1 (MQ=255) aGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTaaa > 6:308389/1‑131 (MQ=255) aGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTaaa < 5:308389/131‑1 (MQ=255) aGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc < 1:40678/203‑1 (MQ=255) aGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCa < 2:326009/228‑1 (MQ=255) gCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGGTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAAc > 8:362692/1‑149 (MQ=255) aaTGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACtt > 7:86545/1‑149 (MQ=255) | AGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGC‑TGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGC > NC_000913/151263‑151778 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |