Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,685,527 A→G 100% G354G (GGT→GGC)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG98.7% 263.4 / ‑4.0 76G354G (GGT→GGC) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (36/39);  minor base C (1/0);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGT  >  NC_000913/1685293‑1685781
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                              
cccTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCa                                                                                                                                                                                                                        >  2:244475/1‑275 (MQ=255)
      gttATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCTCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCGCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGATGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGGTCGCCACGTCGTTCCCCATCACCt                                                                                                                                                                                                                  <  2:96408/274‑1 (MQ=255)
               ttttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCCCCTGAcaccag                                                                                                                                                                                                          >  2:343857/1‑274 (MQ=255)
               ttttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCAGCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                                                                                                                                                                            <  2:12824/272‑1 (MQ=255)
                                   aaaggtattAACCAAGGTGGGTGTTAAGCGCTGTCACCAGCATAAGCGATTCATTGATTAATTGAGTGTTTGGCTAACGATTCGGTTCAATACCCACGGCGAAGTGTTTGTGAAAACTTTCCAGG‑CCATCTGCCAGCAAGCGCACCGAGTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                      <  4:175690/266‑1 (MQ=255)
                                    tttGTCATAACCAATGTGGGTGTTAAACGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGACGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACCCt                                                                                                                                                                                    >  2:190417/1‑275 (MQ=255)
                                             acgaaggtgGGTGTTAAGCGCAGTCACCAGGAGCAGGGATTTATTGAGTAATTGATGGTATCGCTTACGATTCGGTTTAATACCCAATGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGAACCGATTGCATGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                                                                                                                                            <  4:438253/268‑1 (MQ=255)
                                                  tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGTAAGCCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                                                                                                                                          >  2:175692/1‑255 (MQ=255)
                                                            aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCCGAGCATg                                                                                                                                                                                                    >  1:74179/1‑235 (MQ=255)
                                                            aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCACTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTATCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTTTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCTCCCATGTTGATCGCCACGTCCTTCCCCATCCCCTGACAGCAGACCATGGTGTATCCCACAACCTGCTTTTGTTTCACTTTTCCCgcc                                                                                                                                                             >  4:260623/1‑272 (MQ=255)
                                                                              cAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCt                                                                                                                                                                                          >  2:37400/1‑227 (MQ=255)
                                                                                  gATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                      <  4:37400/227‑1 (MQ=255)
                                                                                             tAATTGTTTGATTCCCTAACGATGCGGTGCAAAACCCCCTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCAACATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCGCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAtt                                                                                                                            <  4:244471/274‑1 (MQ=255)
                                                                                                ttgattgaTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                      >  7:89407/1‑149 (MQ=255)
                                                                                                   attgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCTCCGATTGCAGGAAAGTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                                                                                                                                   <  8:35104/149‑1 (MQ=255)
                                                                                                   attgattAACTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAGAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATAGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                                                                                                                                   >  6:370966/1‑149 (MQ=255)
                                                                                                    ttgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGccccc                                                                                                                                                                                                                                                  <  5:226204/149‑1 (MQ=255)
                                                                                                       attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                                                                                                                                                                                    >  4:514778/1‑192 (MQ=255)
                                                                                                       attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCAGGCATGATTGAGCTGCCCGGCTCATTTTCGGGGATTGAGATTTcccc                                                                                                                 >  2:499241/1‑275 (MQ=255)
                                                                                                           gCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                <  2:514783/192‑1 (MQ=255)
                                                                                                             tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGAt                                                                                                                                                                                                                                         <  6:315573/149‑1 (MQ=255)
                                                                                                              cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATc                                                                                                                                                                                                                                        <  7:276645/149‑1 (MQ=255)
                                                                                                                    gttaagagacgaccAATGCGCAGAGTTTTGTAAAACTTTCCAGGCCCATGTG‑CAGGAAGCGCCCCGATTTCAGGAAATTGTTGGTCACCATTGGACGGAAGACGGTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAgcg                                                                                                     <  4:464021/262‑1 (MQ=255)
                                                                                                                        ttCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                                                                                                                                              >  7:112589/1‑149 (MQ=255)
                                                                                                                        ttCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                                                                                                                                              >  8:59086/1‑149 (MQ=255)
                                                                                                                            aTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                                                                                                                                          <  8:115391/149‑1 (MQ=255)
                                                                                                                            aTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                                                                                                                                          >  5:122632/1‑149 (MQ=255)
                                                                                                                             tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGaa                                                                                                                                                                                                                                                          >  3:74430/1‑116 (MQ=255)
                                                                                                                             tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGAGCGCCACGTCGTTcccc                                                                                                                                                                                                                         <  5:343841/149‑1 (MQ=255)
                                                                                                                                 cACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                      <  1:74430/116‑1 (MQ=255)
                                                                                                                                  aCTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGATCTGCCCGGCTCCTTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCgggccaggggccag                                                                                      >  2:172319/1‑275 (MQ=255)
                                                                                                                                  aCTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTACCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAAAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGTTTGTGCTTTCACCAATTCCGCAGCGCGGGCCAGAGgacag                                                                                      >  2:143335/1‑275 (MQ=255)
                                                                                                                                    tGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccagcc                                                                                    >  2:331799/1‑275 (MQ=255)
                                                                                                                                     gcgcAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTCCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTCTCCGGGATTGAGATTTCACCAATTCCGCAGCCCGGGCCAGAGgccagcc                                                                                    >  2:402210/1‑274 (MQ=255)
                                                                                                                                      cgcAGTGTTGGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGACACGTCGTTCCCCATCACCTGa                                                                                                                                                                                                                >  5:409217/1‑149 (MQ=255)
                                                                                                                                        cAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAca                                                                                                                                                                                                              <  7:59086/149‑1 (MQ=255)
                                                                                                                                          gtgtTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCCCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCCGCGGa                                                                              >  2:333467/1‑275 (MQ=255)
                                                                                                                                           tgtttgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgctgtccctttta                                                                              >  2:464026/1‑263 (MQ=255)
                                                                                                                                            gtttgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                                                                                                                                          >  6:321544/1‑149 (MQ=255)
                                                                                                                                             tttgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                                                                                                                                               <  5:144935/111‑1 (MQ=255)
                                                                                                                                             tttgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAt                                                                                                                                                                                                                                               >  6:144935/1‑111 (MQ=255)
                                                                                                                                             tttgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCaga                                                                                                                                                                                                         <  6:455673/149‑1 (MQ=255)
                                                                                                                                               tgttAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                       <  6:131667/149‑1 (MQ=255)
                                                                                                                                                   aaaaCTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                                                                                                                                                                                   <  8:112589/149‑1 (MQ=255)
                                                                                                                                                   aaaaCTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                                                                                                                                                                                   <  8:196714/149‑1 (MQ=255)
                                                                                                                                                   aaaaCTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTgg                                                                     >  2:207403/1‑275 (MQ=255)
                                                                                                                                                    aaaCTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                       <  8:18735/144‑1 (MQ=255)
                                                                                                                                                    aaaCTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                       >  7:18735/1‑144 (MQ=255)
                                                                                                                                                       cTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTaa                                                                                                                                                                                               <  6:332016/149‑1 (MQ=255)
                                                                                                                                                       cTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTaa                                                                                                                                                                                               <  7:270560/149‑1 (MQ=255)
                                                                                                                                                        tttCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                                                                                                                              >  7:337412/1‑149 (MQ=255)
                                                                                                                                                          tCCATG‑CCATCTGCCAGCAAGTGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGc                                                                                                                                                                                            <  7:372437/149‑1 (MQ=255)
                                                                                                                                                           ccATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCCGCCGACATCATTGGCGAtttt                                                              >  2:430881/1‑274 (MQ=255)
                                                                                                                                                               g‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTc                                                                                                                                                                                         <  6:160165/147‑1 (MQ=255)
                                                                                                                                                               g‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTc                                                                                                                                                                                         >  5:160165/1‑147 (MQ=255)
                                                                                                                                                                  cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                                                                                                                                                >  6:435363/1‑122 (MQ=255)
                                                                                                                                                                  cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                                                                                                                                                <  5:435363/122‑1 (MQ=255)
                                                                                                                                                                          aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                                                                                                                                             <  7:406362/149‑1 (MQ=255)
                                                                                                                                                                           tcAAGCACACCGATTGCAGGAAATTGTGGCTCACCATTGGACGGAAGACGTTCAGTTCAACGTTGACGGAATCTCCCCCCTTGTTGATCGCCACGTCGTTCCCCATAACCTGACACCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGGGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                              <  4:331795/274‑1 (MQ=255)
                                                                                                                                                                           gcaggggcACCGATTGCAGGAATGTGTGGGTAACCATTGGACGGAAGACGTTCAGTTCAAATTTGCCGGAAGGGCCCCCCATGGTGATGGCCACGTCGTTCCCCATAACCTGACAGCAGGGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCCGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATGTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                              <  4:343853/269‑1 (MQ=255)
                                                                                                                                                                           gCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                                                                                                                                            <  6:122632/149‑1 (MQ=255)
                                                                                                                                                                           acaagacaaCCGACTGGCGGAAATGGGAGAACACCATTGGACTGATGACGTCCATGTCCAAGTTGCCGGAAGCGGCACCCAGTGTGAGCGCACCGTCGTTCCCCATCCCCGGACAGCAGAGAATGGTTAATGCCTCACACTGTGGTGTGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                              <  4:190415/267‑1 (MQ=255)
                                                                                                                                                                                cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCAc                                                                                                                                                                       <  6:460722/149‑1 (MQ=255)
                                                                                                                                                                                               aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAt                                                                                                                                                        <  8:337412/149‑1 (MQ=255)
                                                                                                                                                                                                         tCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                                                                                                                                              <  6:409217/149‑1 (MQ=255)
                                                                                                                                                                                                             cATTTGACTGAAGACGTTCAGTTCAAAGTTGCCGGCAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCTATCACCTTACAGCAGAGCATGGTTAATGCCTCACACTGTGTCGGGTTCACTTTCCCCGGCACGATTGAGCTGCCCGGCt                                                                                                                                          <  5:370966/149‑1 (MQ=255)
                                                                                                                                                                                                             aaTTGGAAGAAAGAAGTTCAGTGCAAAGTTGCGCGAAGCGCACCCCATGGTGACCGCCACGTCGTTCCCAATCACCTGACAGAAGAGCATGGTTAATGCATCACACTGTGTTGGGTTCACTTGCCCAGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCa               <  1:140500/271‑1 (MQ=255)
                                                                                                                                                                                                                      gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                                                                                                                                 <  8:317252/117‑1 (MQ=255)
                                                                                                                                                                                                                      gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg                                                                                                                                 >  7:379213/1‑149 (MQ=255)
                                                                                                                                                                                                                      gAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTGTccc                                                                                                                                                                 >  7:317252/1‑117 (MQ=255)
                                                                                                                                                                                                                       aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCgg                                                                                                                                >  7:385254/1‑149 (MQ=255)
                                                                                                                                                                                                                       aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGt  >  2:221932/1‑275 (MQ=255)
                                                                                                                                                                                                                             ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTga                                                                                                                          >  7:247743/1‑149 (MQ=255)
                                                                                                                                                                                                                                 gttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGt                           >  4:184350/1‑240 (MQ=255)
                                                                                                                                                                                                                                  ttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAttt                                                                                                                     >  6:442602/1‑149 (MQ=255)
                                                                                                                                                                                                                                     aaaGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTgcgc                       <  2:184352/240‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                              
CCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATG‑CCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGT  >  NC_000913/1685293‑1685781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑