Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,675 C→T 5.6% intergenic (‑150/+29) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6750CT 5.6% 224.1 / 2.2 71intergenic (‑150/+29)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base C (39/27);  new base T (2/2);  total (41/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAC  >  NC_000913/2085516‑2085912
                                                                                                                                                               |                                                                                                                                                                                                                                             
ttGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCGCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAACAAAAGGTTCATTCGACTCCGGTTTATAATTAGAACAGATGATTATGCTACGTCTTCGTACTGAGGACCCGGAGTAAGGAAGCCCTTGGGAACCGCAGCCAGGTAAATCAAAACGaaaacacccaaaa                                                                                                                             >  4:197713/1‑262 (MQ=255)
                 ttaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                                                                                                                         >  5:199763/1‑149 (MQ=255)
                        gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTAACGCAGGCCAGGTAAATCAGACCGATAGCAGCCCAGCTCAGCACCAGAACCATTAAGCTttc                                                                                                    >  4:368822/1‑275 (MQ=255)
                            aaTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattatt                                                                                                                                                                                                                              <  6:476413/149‑1 (MQ=255)
                                            ttattCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATg                                                                                                                                                                                                              >  8:102576/1‑149 (MQ=255)
                                                  aTGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTa                                                                                                                                                                                                                >  8:169802/1‑141 (MQ=255)
                                                  aTGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTa                                                                                                                                                                                                                <  7:169802/141‑1 (MQ=255)
                                                       tggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGCGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGACCCGGATTACGGAAGCTCTTGGTGTCGCAAGCCAGGTAAATCAGACCCATAGCAGCACAGATCAAACCCAGAACCCTTGACCTTTCCTCCCGGTTAACCCACAGCacacaacgcgc                                                                      >  4:352500/1‑267 (MQ=255)
                                                        ggtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCa                                                                    <  2:368827/275‑1 (MQ=255)
                                                         gtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTAt                                                                                                                                                                                                               >  1:24276/1‑135 (MQ=255)
                                                         gtggAATGCAGACCAATAACCAAATTCCCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTATTCCAGGTTAACCAACAGCGCACAAACGGTCAg                                                                   >  2:380669/1‑275 (MQ=255)
                                                           ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAgcg                                                                 <  2:197715/275‑1 (MQ=255)
                                                             aaTNCAGNCCAATAANCAAATTCGCTAAATTAGAACATATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGTAGCCACATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATTATTATGCTa                                                                                                                                                                                                           <  3:24276/135‑1 (MQ=255)
                                                                  aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTTGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAc                                                                                                                                                                                        <  7:456936/149‑1 (MQ=255)
                                                                  aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                                                                                                                          >  5:280737/1‑99 (MQ=255)
                                                                  aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                                                                                                                          <  6:280737/99‑1 (MQ=255)
                                                                    aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCg                                                                                                                                                                                      >  7:26922/1‑149 (MQ=255)
                                                                    aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCg                                                                                                                                                                                      >  7:78139/1‑149 (MQ=255)
                                                                    aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATagt                                                                                                                                     >  2:20998/1‑197 (MQ=255)
                                                                      cAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTAc                                                                                                                                                                                                >  6:76764/1‑137 (MQ=255)
                                                                      cAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTAc                                                                                                                                                                                                <  5:76764/137‑1 (MQ=255)
                                                                                aTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                                                                                                                            >  3:153975/1‑131 (MQ=255)
                                                                                   ctctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTAGTATTCGAAAAGAAGCTTATGCGACGGCTACGCACTGCGGAACCGGATTACGCaaccgct                                                                                                                                                                       <  6:199763/149‑6 (MQ=255)
                                                                                    tctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAc                                                                                                                                                                                        <  1:153975/131‑1 (MQ=255)
                                                                                        aaTTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTg                                                                                                                                                                  >  7:263924/1‑149 (MQ=255)
                                                                                           taaaaaaaaTGTTTATTAATGATGCTTTTGATACAAAAAAATAAAAAAACCTGAGCCGAATGTTCTTTATGATCCGGTTTATTATTAGAAGAGCTGATTATGCTAGGCCTTCGTACTGCGGAACCGGATTACGGAAGAGAGTGGGAACGCAAGCCAGGTAAATCACACCGATAGAAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCAACAGCGTACCAACGGTCAGCGGACCAGACGTCGGCAGGAACAGATACTGGAAg                                 <  4:380664/269‑1 (MQ=255)
                                                                                              aaCAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGa                              >  2:433610/1‑275 (MQ=255)
                                                                                                   aTGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCCCCAACGGTCCGCGCACCACACATCGGAAGGAACAGATACCGGAACTAATCtt                          >  4:311740/1‑274 (MQ=255)
                                                                                                        tattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTAt                                                                                                                                                                                                               >  4:535550/1‑88 (MQ=255)
                                                                                                        tattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                                                                                                                                  >  8:99735/1‑149 (MQ=255)
                                                                                                           taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAAt                                                                                                                                                 >  5:230791/1‑147 (MQ=255)
                                                                                                           taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAAt                                                                                                                                                 <  6:230791/147‑1 (MQ=255)
                                                                                                           taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                                                                                                                       >  1:78546/1‑173 (MQ=255)
                                                                                                            aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                                                                                                                           <  2:535555/88‑1 (MQ=255)
                                                                                                               gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                                                                                                                           <  8:78139/149‑1 (MQ=255)
                                                                                                               gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAg                                                                                                                   <  3:78546/173‑1 (MQ=255)
                                                                                                                 ggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAcc                                                                                                                                                                                       >  1:65353/1‑103 (MQ=255)
                                                                                                                   agTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                                                                                                                             >  4:521168/1‑111 (MQ=255)
                                                                                                                    gTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATag                                                                                                                                      >  8:381423/1‑149 (MQ=255)
                                                                                                                    gTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATag                                                                                                                                      <  7:102576/149‑1 (MQ=255)
                                                                                                                     ttCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGAt                                                                                                                                                                                   <  3:65353/103‑1 (MQ=255)
                                                                                                                     ttCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGTTCAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAACGCCACAACGAGCAACGGACCACAAAGCGGCAGGAACAACTACTGGAAGTAGACTTTCAGACTCTTATTAAGcct       >  4:333889/1‑273 (MQ=255)
                                                                                                                     ttCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTTAGCTTTCTTCCAGGTTAACCCACAGCGAACCAACGGTCAGCGCACCCAACATCCGCAGGACAAAAAACGGGAAGTTATCATTCCGCTTCTTTTTACGcct       >  4:94405/1‑273 (MQ=255)
                                                                                                                     ttCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGACCAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt       >  2:160947/1‑275 (MQ=255)
                                                                                                                       cGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGct                                                                                                                                                                         <  2:521173/111‑1 (MQ=255)
                                                                                                                          tACAAAAGAATAAAATAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCtt                                                                                                                                                                                                     >  8:455077/1‑80 (MQ=255)
                                                                                                                          tACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCtt                                                                                                                                                                                                     <  7:455077/80‑1 (MQ=255)
                                                                                                                          tACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTAGGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc  <  4:433605/275‑1 (MQ=255)
                                                                                                                             aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAAt                                                                                                                                                 >  6:425829/1‑129 (MQ=255)
                                                                                                                             aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAAt                                                                                                                                                 <  5:425829/129‑1 (MQ=255)
                                                                                                                             aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGa                                                                                                                             >  7:451801/1‑149 (MQ=255)
                                                                                                                              aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                                                                                                                            >  6:292069/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                                                                                                                        <  7:381423/149‑1 (MQ=255)
                                                                                                                                   aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                                                                                                                               <  6:89493/125‑1 (MQ=255)
                                                                                                                                   aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                                                                                                                               >  5:89493/1‑125 (MQ=255)
                                                                                                                                   aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                                                                                                                       >  5:460553/1‑149 (MQ=255)
                                                                                                                                       aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAg                                                                                                                   >  6:315556/1‑149 (MQ=255)
                                                                                                                                        aaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGCCGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                    <  4:474492/243‑1 (MQ=255)
                                                                                                                                           aCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc                                                                                                               >  8:387085/1‑149 (MQ=255)
                                                                                                                                              ggAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAg                                                                                                                                                       >  5:450239/1‑106 (MQ=38)
                                                                                                                                              ggAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAg                                                                                                                                                       <  6:450239/106‑1 (MQ=38)
                                                                                                                                                    aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGaa                                                                                                                 <  5:445594/138‑1 (MQ=255)
                                                                                                                                                    aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGaa                                                                                                                 >  6:445594/1‑138 (MQ=255)
                                                                                                                                                    aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa                                                                                                                  >  5:67053/1‑137 (MQ=255)
                                                                                                                                                    aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa                                                                                                                  <  6:67053/137‑1 (MQ=255)
                                                                                                                                                      aTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTttc                                                                                                    <  8:451801/149‑1 (MQ=255)
                                                                                                                                                          tCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGAt                                                                                                                                        <  8:374617/109‑1 (MQ=255)
                                                                                                                                                          tCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGAt                                                                                                                                        >  7:374617/1‑109 (MQ=255)
                                                                                                                                                            aTTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGAc                                                                                                                                                                <  8:230981/83‑1 (MQ=255)
                                                                                                                                                            aTTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGAc                                                                                                                                                                >  7:230981/1‑83 (MQ=255)
                                                                                                                                                             gtCGAATCCGGATTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTAGTGACGCATGCCAGGTAAATCAGAACGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAgg                                                                                             >  6:9691/2‑149 (MQ=255)
                                                                                                                                                               cGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGtt                                                                                           <  7:99735/149‑1 (MQ=255)
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TTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAC  >  NC_000913/2085516‑2085912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: