| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,621 | A→G | 10.4% | intergenic (+87/‑109) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,621 | 0 | A | G | 10.4% | 244.3 / 17.5 | 87 | intergenic (+87/‑109) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (43/35); new base G (4/5); total (47/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-03 | |||||||||||
TGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCAAAG > NC_000913/164356‑164895 | tGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCTCACCCTAACCCTCTCCCTCAAggggaag > 2:442918/1‑270 (MQ=255) tGGAGTTGCTTTAACCGTCCCAAAGGCCATTACAAATCACACGAGATTGACGCGACGGCTGGAAAGGGGCGTACCGTGGGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCGGGCCGGACGGCCCGGCAAATACTGCACCGACGCGACGGAAGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGTGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgggt < 4:446251/274‑2 (MQ=255) tGCTTCCACCGTCCCAAAGGCCATTACAGATCACACCAGTGTTGAGCGACTTCTGGAAAGTGGCGTACCGTGAGGTGCAAAAAGGGATGAAAGGGCGTTATCACAAACACGTCTGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCGTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTTCACCCTCACCCTTTCCCTCTCCCTCAAGGGAGAGGGGACCGAt < 4:259678/274‑1 (MQ=255) aTCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATAAGCGCGCCCGGTCACACTCACCCTCACCCACTCCCTCAAGTGAGAGGGGACCGATCGGG‑ACAAAATGTTGAGA‑GATCtcttcctc > 4:112615/1‑275 (MQ=255) gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCCACAAA‑TTTGAGA‑GATCTCTTCTCCTGTCTTGTCACCGA‑AGAAcctgtctcttat > 4:133498/1‑263 (MQ=255) aGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGCGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCt > 2:23442/1‑275 (MQ=255) gTACCGTGAGGTGCAAAAAGGGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGt < 2:133499/248‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTg > 2:513762/1‑219 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAC‑CTCAAATATTGAGA‑GATATCTTCTTCTGTCTGGTAACAGA‑CTAACAAAAAATCCGGCTTTTGCGCCTGAATATTGCg > 4:398795/1‑275 (MQ=255) gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa > 2:209396/1‑275 (MQ=255) tGANNGGGCGTTATCCCAAACATGGCTGGCCGGACGACCCGGCAAATACTGCANCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgaga‑g < 4:513757/206‑1 (MQ=255) agtgCGTTAGCCCAAGCAGGTCTGGCCGGACGGCCAGGAAAATAAGGGACCGAGGCGAAGGATGAAAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCGGGGGGGGAGGGCCGGCGTGAGGGCATCAGCGCGCACGTTCACTCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAG‑GAAAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTACCAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAGAAGc < 4:23442/271‑1 (MQ=255) aTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCAACCTAACCCCCTCCCTCAAGGGAGAGGGGACCGAACGAG‑CCAAAAGTTTGAGA‑GATATCTTCTTCTGTCTTGTTAAAGA‑AGAACAGACAACCGGGCTTTTGCGCCTGAATATTGAGGAAAAAAAACATGGCCcgg > 4:85601/1‑275 (MQ=255) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg < 2:112616/274‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 8:315993/149‑1 (MQ=255) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCACCTCCCTGTGGGCGATGGCCTGGGTGAGGGCATCAGCGCGCCAGCTCATCCTCACCCTAACCCTCCCCCTAAAGGGAGAGGGGGCCGACCGAG‑CACAAA‑CTTGATACGCTCTCTTCTTCTGTCGTTTACCAGACACAAC‑GAAAATAGTGTTTGTGAGCCTGCATATTGCGGGGAAAAAGCAGGACCGGTAATGGccaggac > 4:193408/1‑270 (MQ=255) tGGCCGGACTACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTAGGAAGGGTGGTTCTCTCCCTCTCCCTCTGGTAGAGAGCCGGGGAGAGGGCAGCAGCGCGAACGTTAACCCTTACCCTAACGCTACCTCTCAAGGGAGAGGGGACCCATGCAG‑CACAACATTTGAGA‑GTTATCTTCTTCTGTCCTGTAACAGA‑CGAACAGACAATCCGCGTTTTCCGCTCGAATCGGGCGGTAAAAAAGCACGCCCGGGACGTACCCCGAGcc > 4:302312/1‑275 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 6:364/149‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag > 7:150318/1‑148 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 8:150318/148‑1 (MQ=255) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc < 2:85602/275‑1 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAtttt > 7:191583/1‑149 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCACGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTcc > 2:81427/1‑275 (MQ=255) cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGC‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATTTTGCGGAGAAAAAGCATTGCCGGGAATGACCGCGAGACAAGTGGACGCAAAGGGAAACCGACCCGTCCggg > 4:215484/1‑274 (MQ=255) cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑Gat < 6:419271/149‑1 (MQ=255) cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGt < 2:302316/275‑1 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg > 7:307252/1‑119 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 8:307252/119‑1 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTc < 2:416390/275‑1 (MQ=255) tATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCtt > 7:467874/1‑149 (MQ=255) aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTgg > 2:474755/1‑232 (MQ=255) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag > 8:375870/1‑99 (MQ=255) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 7:375870/99‑1 (MQ=255) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAAc > 5:140671/1‑149 (MQ=255) tAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgtc < 2:193410/274‑1 (MQ=255) aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACaga < 6:353147/149‑1 (MQ=255) ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga < 5:47830/118‑1 (MQ=255) ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga > 6:47830/1‑118 (MQ=255) ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACaga‑aga > 5:270941/1‑149 (MQ=255) ggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAAc < 8:191583/149‑1 (MQ=255) tctccttcccctCGTGGGAGAGGGGCGGCGGGAGGTCGTCCGCGCGCGCGTTCACCCGAACCCTAACCCTATCCCTCAAGGGGGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GGTATCTTCTTCTGTCTTGTAAAAGA‑AGAACAGAAAATCGGGCTTGTGCGCCTGAATAGTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGTAAAGGGAAACCGACGCGTCCGGTCAATCAAAAGGGAAGCCGTCGTCGTTACGAAGa < 4:156042/275‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAAc > 2:523507/1‑129 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACaga‑a < 4:523502/129‑1 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCtttt > 5:202333/1‑149 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAt > 6:390959/1‑94 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAt < 5:390959/94‑1 (MQ=255) gtgGGGGAGGGACGGGGCTAGGGGTTCAGCGCGCACGTCCAACGTCCACCTAACCCATGCCCTAAATGGAGAGGGGAACGATCGAG‑AACAAATTTTGAGA‑GTTATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGTTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTac < 4:81426/275‑1 (MQ=255) gtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTgc < 6:270941/149‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTCCGAAGATGACgataatga > 2:219723/1‑271 (MQ=255) gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTacga < 2:249337/275‑1 (MQ=255) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGCAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCCAGCCAATTGGAGGCAAAGGGAAACCGACGCGTCCGGTCAACCAAAAGGTCAGCCGGCGTCGTTACGAAGATGACGATGAttcagaaga > 2:61443/1‑268 (MQ=255) gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgag < 6:294698/93‑1 (MQ=255) gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgag > 5:294698/1‑93 (MQ=255) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCAGGTCAAACAAAAGGTAAGCAGTCGTCGTTACGAAGACGACGATACTTacgacagtt > 2:85314/1‑271 (MQ=255) gCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTatg < 2:215487/275‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCt > 4:105758/1‑123 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 6:140671/149‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCgg > 8:466149/1‑116 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCgg < 7:466149/116‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTg < 2:105759/123‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCg > 4:406163/1‑54 (MQ=255) aGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATg < 8:467874/149‑1 (MQ=255) gcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg < 2:406168/54‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGCAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa > 7:260745/1‑149 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa < 5:234477/149‑1 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg > 5:74316/1‑135 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 6:74316/135‑1 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCGTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATg < 2:161412/274‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATAACcgc > 5:16406/1‑149 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGTACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGt < 2:20756/275‑1 (MQ=255) ctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 5:264178/1‑149 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGAGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGAtt > 1:53795/1‑221 (MQ=255) cccTCTCCCTTGAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 7:236097/140‑1 (MQ=255) cccTCTCCCTTGAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 8:236097/1‑140 (MQ=255) ccTCTCCCTTGAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACa < 6:182159/78‑1 (MQ=255) ccTCTCCCTTGAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACa > 5:182159/1‑78 (MQ=39) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGCGCCAATTGGa < 8:260745/149‑1 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa > 7:356398/1‑149 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATACTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa > 8:458277/1‑149 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa > 7:469806/1‑127 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa < 8:469806/127‑1 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGC‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTACg > 1:12496/1‑169 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTAAGACGATTATGATGAATATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAg > 1:63575/1‑268 (MQ=255) ccccaaaaGGGAGGGGGGACCGAGAGNN‑CAAAAATGTTGGCA‑GATTGTTTCTTCTGTCNTGTAACAGA‑AGAAACGAAAATCGGGCTTTGGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCNAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggca < 3:63575/266‑1 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaaa > 6:192461/1‑149 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaaa > 7:23391/1‑149 (MQ=255) tCAAGGGAGAGGGGACCGATCGAG‑CACAACTTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCTGGCTTTTGCGCCTGAATATGGCGGAGAAAAAGCATGGCCGGGACTGACGGCGAGACAATTAGAAGCAAAGGGAGACAGACGCATACGGTAACACAAAAGGTACGCGGACTGCGTTACGAAAATTACGAAGATTCCGACGTGTATGCTACCTATATCGATGAAAAACGGATTCCGAGCAGAGGAAAgagataagcgag > 4:498795/1‑264 (MQ=255) tCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 8:419722/1‑149 (MQ=255) tCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggcaaaggcaa < 2:398800/275‑1 (MQ=255) aaGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCAAATTGGACGCAAAAGGAAACCGACGCGACAGGTCCAACAAAAGGTAAGCCGTCGTCGTTACGAAGNAGACGATGATTACGACGAATATGATGACGAAGAGGCTGAAGAATAGATGCCACGAAAAGGGAAGggcaaagagaaaa > 3:111148/1‑269 (MQ=255) | TGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGA‑GATATCTTCTTCTGTCTTGTAACAGA‑AGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCAAAG > NC_000913/164356‑164895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |