Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,123,637 T→A 5.2% E295V (GAG→GTG)  cpsB ← mannose‑1‑phosphate guanyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,123,6370TA 5.2% 264.1 / 4.5 78E295V (GAG→GTG) cpsBmannose‑1‑phosphate guanyltransferase
Reads supporting (aligned to +/- strand):  ref base T (31/41);  new base A (2/2);  total (33/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

TCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTCACAGGCATCGAGGATATCCGGGCGATATTTTTTCAGTTCTTCGAGATAGCGT  >  NC_000913/2123418‑2123888
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                           
tCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTTGGCGCTCATTCCCCATAATGAAGACCAGGAGCCAACATCGCTCCa                                                                                                                                                                                                      >  2:169336/1‑275 (MQ=255)
tCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCa                                                                                                                                                                                                      >  2:224649/1‑275 (MQ=255)
      tGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCTCTCCAGCCCGc                                                                                                                                                                                                >  1:19665/1‑275 (MQ=255)
                gCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTTGGCGGTGTGGGCGCTGACCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCgg                                                                                                                                                                                       <  2:99811/274‑1 (MQ=255)
                         gACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaaca                                                                                                                                                                             >  2:499765/1‑275 (MQ=255)
                                      gttCTGCTCGACCCCTTTTTTGCCATCCTGTACCGCTTTAGGGTTGGAAATTAGCCCCGCATCTTGTGGCCTCACCACTTCCAGATCTTTCCCCCCGGCGGGGGTGACCAGGGCGGATTCAGCATACACATAGCTGTTTTCAGGTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGTCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGGGCCACCATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTa                                                                                                                                                                 <  4:499760/272‑1 (MQ=255)
                                            cTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCCTAATGAAGACCAGGGGCCAACATCGCTCCAGCCCGCATCCCTCGGCCACACCACAGCATCTgtcccttaaaca                                                                                                                                                           >  1:135454/1‑263 (MQ=255)
                                              gcccctcTTTTTTCCCTCCGTTACCCCTTTACGTGCGGCAATCAGACCCGCTACTTTGGTCTGCACCACTACCACATCTTTCACCCCGACGGTGATGACCAGGCCAGATTCAGCATACACATAGCTGTGTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGa                                                                                                                                                       <  4:224645/269‑1 (MQ=255)
                                                gccACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTa                                                                                                                                                                 <  2:21405/263‑1 (MQ=255)
                                                               tCCTTTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCGGATCTTTCACCCCGGCGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGa                                                                                                                                       <  2:401240/275‑1 (MQ=255)
                                                               tCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGATGATCTCCCATAATGAAGACCAGGAGCCAACATCGCCCCAGCCCGCATCCCACCGCACCACAACAGCATCTGCCTTAACGTCCATGACCGCGGAATCccccaa                                                                                                                                       >  4:390889/1‑273 (MQ=255)
                                                                        gcgTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAg                                                                                                                                                                                                                            >  4:52797/1‑181 (MQ=255)
                                                                        gcgTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCg                                                                                                                               >  2:347092/1‑274 (MQ=255)
                                                                         cgTTACGGTCGGCAACCAGAACGCCATCTTTGGCGTGACCCCACACCAGTTCTTTCACCCCGACGTGGGTGACCAGGCCGGATTCGTCATACCAATAGCTGGTTTCAGTTGTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCGCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCggg                                                                                                                             <  4:184348/275‑1 (MQ=255)
                                                                           ttACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGACGTGGCAAACGTTGACCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCACTCCAGCCCGCATCCATCGGCCCCCCCACCGCATCTGCGCGCCGTTCCTTTACCCCGAGACCCCACAACCCTTCCGGGCa                                                                                                                           >  4:495171/1‑275 (MQ=255)
                                                                            tACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCa                                                                                                                                                                                                                        <  2:52798/181‑1 (MQ=255)
                                                                            tACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACa                                                                                                                                                                                                              <  2:228823/191‑1 (MQ=255)
                                                                                   ggCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCgaga                                                                                                                    >  2:128781/1‑274 (MQ=255)
                                                                                   ggCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGaa                                                                                                                   <  2:390894/275‑1 (MQ=255)
                                                                                       aTCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCt                                                                                                                                                                                                                                             <  8:118159/149‑1 (MQ=255)
                                                                                        tCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTg                                                                                                                                                                                                                                            >  7:290387/1‑149 (MQ=255)
                                                                                           gCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGAtc                                                                                                                                                                                                                                         <  5:114979/149‑1 (MQ=255)
                                                                                             aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGAtctc                                                                                                                                                                                                                                       <  5:403465/149‑1 (MQ=255)
                                                                                                 cATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCAt                                                                                                                                                                                                                                   <  8:290387/149‑1 (MQ=255)
                                                                                                    cTTTGGTCTGCACCACTACCACATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCAc                                                                                                   <  2:495176/274‑1 (MQ=255)
                                                                                                        tGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGaa                                                                                                                                                                                                                             <  5:295161/149‑1 (MQ=255)
                                                                                                        tGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGaa                                                                                                                                                                                                                             <  8:93352/149‑1 (MQ=255)
                                                                                                        tGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGaa                                                                                                                                                                                                                             <  7:97429/149‑1 (MQ=255)
                                                                                                        tGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAAAGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGaa                                                                                                                                                                                                                             <  8:276617/149‑1 (MQ=255)
                                                                                                           tCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGAc                                                                                                                                                                                                                          <  6:26262/149‑1 (MQ=255)
                                                                                                             tGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGc                                                                                                                                                                     >  4:192772/1‑200 (MQ=255)
                                                                                                                  ccacTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTa                                                                                                                                                                 <  2:192774/200‑1 (MQ=255)
                                                                                                                      tACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCaa                                                                                                                                                                                                                <  8:39598/149‑1 (MQ=255)
                                                                                                                      tACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCAcggcgat                                                                                                                                                                                                                                                      <  8:442261/111‑3 (MQ=37)
                                                                                                                        ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTccc                                                                                                                                                                                                                                     >  7:245586/1‑126 (MQ=255)
                                                                                                                        ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTccc                                                                                                                                                                                                                                     <  8:245586/126‑1 (MQ=255)
                                                                                                                        ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACa                                                                                                                                                                                                              >  7:282364/1‑149 (MQ=255)
                                                                                                                        ccAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCAcggcgat                                                                                                                                                                                                                                                      >  8:46427/1‑107 (MQ=37)
                                                                                                                         cAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACAt                                                                                                                                                                                                             >  6:219764/1‑149 (MQ=255)
                                                                                                                         cAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACAt                                                                                                                                                                                                             <  5:190118/149‑1 (MQ=255)
                                                                                                                          aGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCa                                                                                                                                                                                                                 <  6:142385/144‑1 (MQ=255)
                                                                                                                          aGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCa                                                                                                                                                                                                                 >  5:142385/1‑144 (MQ=255)
                                                                                                                          aGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTa                                                                                                                                                                 <  1:143699/192‑1 (MQ=255)
                                                                                                                            aTCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGc                                                                                                                                                                                                          >  7:319480/1‑149 (MQ=255)
                                                                                                                              cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                                                                                                                        <  7:184544/149‑1 (MQ=255)
                                                                                                                                ttCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCt                                                                                                                                                                                                                                             >  2:281937/1‑110 (MQ=255)
                                                                                                                                ttCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAGACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGAc                                                                        >  2:72413/1‑275 (MQ=255)
                                                                                                                                    ccccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGAtc                                                                                                                                                                                                                                         <  4:281933/110‑1 (MQ=255)
                                                                                                                                      ccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCACGGCGAtgtgattaa                                                                                                                                                                                                                                              >  8:78586/1‑98 (MQ=21)
                                                                                                                                      ccGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACACCGCCGAGGGCAACGTTTGCCACGGCGAtgtgattaa                                                                                                                                                                                                                                              <  7:78586/103‑6 (MQ=18)
                                                                                                                                        gACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCgaga                                                                                                                    >  4:233077/1‑223 (MQ=255)
                                                                                                                                            gtggtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACg                                                                                                                <  2:233081/223‑1 (MQ=255)
                                                                                                                                             tggtgACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCggg                                                                                                                             <  1:44058/209‑1 (MQ=255)
                                                                                                                                                  aCCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGgtg                                                                                                                                                                                                                                                     >  2:507341/1‑84 (MQ=255)
                                                                                                                                                   ccAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGa                                                                                                                                       <  2:308831/193‑1 (MQ=255)
                                                                                                                                                     aGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTcaca                                                   <  2:445344/275‑1 (MQ=255)
                                                                                                                                                     aGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCACATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTcaca                                                   <  2:304102/275‑1 (MQ=255)
                                                                                                                                                      ggCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTggg                                                                                                                                                                                                                                                 <  4:507336/84‑1 (MQ=255)
                                                                                                                                                         cAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaaca                                                                                                                                                                             <  5:219764/149‑1 (MQ=255)
                                                                                                                                                             ttCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCAt                                                                                                                                                                         >  5:370387/1‑149 (MQ=255)
                                                                                                                                                                   aTACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                                                                                                                        >  7:348320/1‑112 (MQ=255)
                                                                                                                                                                   aTACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTc                                                                                                                                                                                                        <  8:348320/112‑1 (MQ=255)
                                                                                                                                                                       acaTAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACg                                                                                                                                                               >  7:363979/1‑149 (MQ=255)
                                                                                                                                                                               gTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTa                                                                                                                                                                 <  2:306750/139‑1 (MQ=255)
                                                                                                                                                                                ttttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGcatc                                                                                                                                                                                             >  2:167196/1‑110 (MQ=255)
                                                                                                                                                                                ttttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaaca                                                                                                                                                                             >  6:467121/1‑126 (MQ=255)
                                                                                                                                                                                ttttCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCacaaca                                                                                                                                                                             <  5:467121/126‑1 (MQ=255)
                                                                                                                                                                                    cAGTTTTGAGATTAAGCACATAGCCGTGGCAAACGTTGGCCGCGGCGGTGTGGGGGCTGATCTCCCATAATGAAGACCAGGAGGCAACATCGATCAAGCCCGaatccatc                                                                                                                                                                                         <  4:167194/110‑1 (MQ=255)
                                                                                                                                                                                     aGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGt                                                                                                                                                 <  6:187178/149‑1 (MQ=255)
                                                                                                                                                                                            tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGAcc                                                                                                                                                     <  7:453187/138‑1 (MQ=255)
                                                                                                                                                                                            tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGAcc                                                                                                                                                     >  8:453187/1‑138 (MQ=255)
                                                                                                                                                                                            tgATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCAc                                                                                                                                          >  6:35118/1‑149 (MQ=255)
                                                                                                                                                                                               ttAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCGAGCCAGCATCCATCGGCACCACAACAGCATCTGCCGTAAGTTCCATGACCGCGTAATACACCGa                                                                                                                                       >  8:240142/1‑149 (MQ=255)
                                                                                                                                                                                                      caTCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTCACAGGCATCGAGGATATCCGGGCGATATTTTTTCAGTTCTTCGAGATAGCGt  >  2:75946/1‑275 (MQ=255)
                                                                                                                                                                                                             gTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCGCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTCACAGGCa                                               <  1:46609/223‑1 (MQ=255)
                                                                                                                                                                                                              tGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGc                                                                                                                        <  5:35118/149‑1 (MQ=255)
                                                                                                                                                                                                                         gCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGctt                                                                                                             <  6:370387/149‑1 (MQ=255)
                                                                                                                                                                                                                           ccTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGcttct                                                                                                           >  6:82263/1‑149 (MQ=255)
                                                                                                                                                                                                                             tCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGcttct                                                                                                           >  8:350937/1‑147 (MQ=255)
                                                                                                                                                                                                                             tCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGcttct                                                                                                           <  7:350937/147‑1 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                           
TCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGGCGGTGTGGGCGCTGATCTCCCATAATGAAGACCAGGAGCCAACATCGCTCCAGCCCGCATCCATCGGCACCACAACAGCATCTGCCGTACGTTCCATGACCGCGTAATCCACCGACTCTTCCGGGCAGGCGAGAAACGCTTCTTCATCCACGCGAATAAAATTGAGATCCGGATCGACGGCGCTCATCGCTTTTTCACAGGCATCGAGGATATCCGGGCGATATTTTTTCAGTTCTTCGAGATAGCGT  >  NC_000913/2123418‑2123888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: