Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,400 G→A 5.4% R405W (CGG→TGG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4000GA 5.4% 300.8 / 5.8 94R405W (CGG→TGG) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (48/40);  new base A (3/2);  total (52/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-02

GTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCGAAGAAATGACCGGGCGCGGCAACGTTGA  >  NC_000913/2140131‑2140664
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                          
gTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCCCAACGTTTTCCAGCCTTTGCt                                                                                                                                                                                                                                                                       >  2:35641/1‑275 (MQ=255)
                                                 ccATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATAGGATAGTTAAACGCATTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCGGGAAGTTTATCCGCGGATTTATTGATTTCGCGTCAAGCGTGCCCGGCa                                                                                                                                                                                                                       >  1:56330/1‑274 (MQ=255)
                                                         ctctACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCCCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCACGGTGCCCGGCAGGTAAACCt                                                                                                                                                                                                              >  4:208856/1‑275 (MQ=255)
                                                                        aaTCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCTTCCAGCCTGCCCGGCAGTGAAACCTTTCCCCCGGTAAGtt                                                                                                                                                                                               >  2:375213/1‑275 (MQ=255)
                                                                                      aGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCAGCGGATTTATTGATGTCCCGTCCAGCGTGCCCGGCAGTGAAACCTGTCAACCGTTCAGTTTGCCCttcgttcga                                                                                                                                                                                 >  4:477618/1‑267 (MQ=255)
                                                                                                  ccATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGc                                                                                                                                                                     >  2:232661/1‑275 (MQ=255)
                                                                                                       cgcgTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAAcc                                                                                                                                                                >  2:165412/1‑275 (MQ=255)
                                                                                                            gtCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCATTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGa                                                                                                                                                             >  2:285650/1‑273 (MQ=255)
                                                                                                                      tCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAACTCGGATAGTTAAACGCCTTCAGACTGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCCGTGAAACCTGTCCACCGTTACGTTTGCCCTGCCGTTGAGTAATTTCACGCAAACCCGACttgtttttct                                                                                                                                                  >  4:210891/1‑269 (MQ=255)
                                                                                                                        gACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                                                                                                                                                                                                       <  2:455146/187‑1 (MQ=255)
                                                                                                                                ccggTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGc                                                                                                                                        <  2:89657/271‑1 (MQ=255)
                                                                                                                                 cTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCg                                                                                                                                                              <  2:340887/251‑1 (MQ=255)
                                                                                                                                   gTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGGAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaa                                                                                                                                                                                                                                                                  <  5:352135/149‑2 (MQ=255)
                                                                                                                                       ttGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTa                                                                                                                                                                                                                                                              >  6:288020/1‑149 (MQ=255)
                                                                                                                                       ttGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTa                                                                                                                                                                                                                                                              >  8:161190/1‑149 (MQ=255)
                                                                                                                                       ttGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTCTTCCAGCCACGGCTGGaaaacgt                                                                                                                                                                                                                                                              >  5:209633/1‑144 (MQ=255)
                                                                                                                                        tGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGCATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGACGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTCCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCCGTTGAGTAATTTCCAGCAAACCCGACTCGTTGGTCCTTTGCGAGGCAAAATCTGt                                                                                                                               >  2:179695/1‑275 (MQ=255)
                                                                                                                                         gCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGt                                                                                                                               >  2:304101/1‑274 (MQ=255)
                                                                                                                                          ccAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACCTTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATcc                                                                                                                                                                                                                                                           <  8:179284/149‑1 (MQ=255)
                                                                                                                                              ttGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGCAACCTTTCCACCGTTACGTTTGCCCTGCAGTTCAGTACTTTCCAGCAAACCCGCCTTGTTGTTCATTTTCGTGGCAACCTCTGTAAAatt                                                                                                                         >  4:464125/1‑275 (MQ=255)
                                                                                                                                                gtgGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                                                                                                                                                                                              >  1:138286/1‑172 (MQ=255)
                                                                                                                                                     ggAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                                                                                                                                                                                                <  5:127475/149‑1 (MQ=255)
                                                                                                                                                        aTGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGAt                                                                                                                                                                                                                                             <  7:224294/149‑1 (MQ=255)
                                                                                                                                                          gCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGt                                                                                                                                                                                                                                           <  6:361505/149‑1 (MQ=255)
                                                                                                                                                               gtcactgttGTAAGCACCGAAGAGATCACATGTCAGTGGGATTTGTCCGGGTATTGAATTAGGGTAGGTGCGCGCCTTTCGAATAGTACCATACGAACCGTTTGACAGCGGTGGCTGGAAGTTTATCCTCGGTTTTATTGAGGTCGCGTCCGGGGTGCCCGACAGTTAAACATGTCGACCGTTAAGGTTGCCCTGTAGTTGAGTAATTTCCAGCAAACCCGTCTTGTTGGTCATTTGCCTGGCGACATCTGTAAAATTCATTCCGCGCCAGCGCa                                                                                                        <  4:461910/267‑1 (MQ=255)
                                                                                                                                                                gCATCTATGTCAGCACCGGAGAAATCGCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCa                                                                                                        <  2:464130/274‑1 (MQ=255)
                                                                                                                                                                gCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTGATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCa                                                                                                        <  2:208859/274‑1 (MQ=255)
                                                                                                                                                                gCATATATGACAAAACCGGGGAAATTCCCCGCCAGTGACAATTTTCCGGTCAACCAAATCGGTTAGGTAAACGCTATCAGAATGCAACCTATCTCAGCGTCGTCCAGCCGGGTCTTGCAGTTTATCAGGGGAGTTATTGATGTAGAGTCGAGCGTGCCCGGCAATGGAAACTAACCACCGTTAGATTTGCCCAGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTAGGTCATTTGCGTGGCAACATCTGTAAAGTTCATTCCGCACAAGCGCa                                                                                                        <  4:235073/274‑1 (MQ=255)
                                                                                                                                                                 cATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                                                                                                                                                                                                    >  5:20141/1‑149 (MQ=255)
                                                                                                                                                                  aTCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTa                                                                                                                                                                                                                                                  <  5:194804/134‑1 (MQ=255)
                                                                                                                                                                  aTCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTa                                                                                                                                                                                                                                                  >  6:194804/1‑134 (MQ=255)
                                                                                                                                                                  aTCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                                                                                                                                                                                                   >  6:11144/1‑149 (MQ=255)
                                                                                                                                                                    cTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGc                                                                                                                                                                                                                                 <  5:11144/149‑1 (MQ=255)
                                                                                                                                                                     gaTGTCAGCACCGGAGGAATCACCAGCCAGTGACATGTTTCCGGTCAACGAAATCGGATAGTTAAACCCCTTCGGGATGGTAGCAATCTCAACGTTTTCCAGCCGTGGCTGGAGGTTTATCCGCGGGTTTATGGGGGTCGCGTCCAGCGTGCCAGGCAGTGAAACCTGTCCACGGTTAAGTTTAGCCTGCAGTTGGGGAATTTCCAGCAAACCCGACTTGGAGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGGGCGTTa                                                                                                   <  4:232657/273‑1 (MQ=255)
                                                                                                                                                                      aTGTCAGCGACGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTAc                                                                                                  <  1:73658/274‑1 (MQ=255)
                                                                                                                                                                      aTGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGt                                                                                                                                                                                                                                                                           >  5:313338/1‑105 (MQ=255)
                                                                                                                                                                      aTGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGt                                                                                                                                                                                                                                                                           <  6:313338/105‑1 (MQ=255)
                                                                                                                                                                                cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGcaa                                                                                      <  2:210894/274‑1 (MQ=255)
                                                                                                                                                                                    gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTgg                                                                                                                                                                                                                                                                         <  7:11001/93‑1 (MQ=255)
                                                                                                                                                                                    gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTgg                                                                                                                                                                                                                                                                         >  8:11001/1‑93 (MQ=255)
                                                                                                                                                                                    gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaaa                                                                                                                                                                                                                 <  5:114332/149‑1 (MQ=255)
                                                                                                                                                                                         cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                                                                                                                                                                                                >  8:322358/1‑113 (MQ=255)
                                                                                                                                                                                         cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                                                                                                                                                                                                <  7:322358/113‑1 (MQ=255)
                                                                                                                                                                                         cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                                                                                                                                                                                            <  6:21813/149‑1 (MQ=255)
                                                                                                                                                                                          aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTc                                                                                                                                                                                                           <  7:441052/149‑1 (MQ=255)
                                                                                                                                                                                                acttggcATTTTTCCGGCAAACGGATGCGGGAAGTTAAACGCGTTCAGAATGGTACCAATCTTAACGTTTTCACGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGCGGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTGACTGGCCTGCAACAAAATATCAGCCGTa                                                                      <  4:304097/268‑1 (MQ=255)
                                                                                                                                                                                                   tGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGACGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCAACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCTGCCTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTACGCGCATCCGCACGTTCATGGCCGGCAAACAAAttatatccttaaacc                                                                   >  4:65398/1‑264 (MQ=255)
                                                                                                                                                                                                     aCATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                                                                                                                                                                                                >  6:272717/1‑149 (MQ=255)
                                                                                                                                                                                                       aTTTTTCCGGTCAACGAAATCGGATAGTTAAACGTCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATACGCGGATTTATTGATGTCGCGTCCAGCGTGcc                                                                                                                                                                                                                            >  8:182194/1‑119 (MQ=255)
                                                                                                                                                                                                       aTTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGcc                                                                                                                                                                                                                            <  7:182194/119‑1 (MQ=255)
                                                                                                                                                                                                       aTTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGttt                                                                                                                                                                                              >  6:244794/1‑149 (MQ=255)
                                                                                                                                                                                                       aTTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTg                                                              <  2:477623/275‑1 (MQ=255)
                                                                                                                                                                                                         ttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGc                                                                                                                                                                                            <  5:288020/149‑1 (MQ=255)
                                                                                                                                                                                                         ttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATCTCAGCCGTAAAGCCTTGc                                                             >  2:440437/1‑274 (MQ=255)
                                                                                                                                                                                                            tCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt                                                                                                                                                                                         >  8:10885/1‑149 (MQ=255)
                                                                                                                                                                                                              cGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                                                                                                                                                                                       >  5:210035/1‑149 (MQ=255)
                                                                                                                                                                                                                 tCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                                                                                                                                                                                                >  1:53386/1‑121 (MQ=255)
                                                                                                                                                                                                                    aCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGa                                                                                                                                                                                 <  5:283113/149‑1 (MQ=255)
                                                                                                                                                                                                                     cGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                                                                                                                                                                                            <  3:53386/121‑1 (MQ=255)
                                                                                                                                                                                                                          tCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGa                                                                                                                                                                                 <  5:461720/143‑1 (MQ=255)
                                                                                                                                                                                                                          tCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGa                                                                                                                                                                                 >  6:461720/1‑143 (MQ=255)
                                                                                                                                                                                                                              aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt                                                                                                                                                                                   >  8:431059/1‑137 (MQ=255)
                                                                                                                                                                                                                              aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt                                                                                                                                                                                   <  7:431059/137‑1 (MQ=255)
                                                                                                                                                                                                                                  ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGa                                                                                                                                                                                 >  8:421388/1‑135 (MQ=255)
                                                                                                                                                                                                                                  ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGa                                                                                                                                                                                 <  7:421388/135‑1 (MQ=255)
                                                                                                                                                                                                                                  ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCaa                                                                                                                                                                   >  7:404361/1‑149 (MQ=255)
                                                                                                                                                                                                                                  ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGGTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTGGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGAACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAAt                                   <  2:451061/275‑1 (MQ=255)
                                                                                                                                                                                                                                   tAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCaaa                                                                                                                                                                  <  7:10885/149‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAAc                                                                                                                                                                 >  5:230107/1‑149 (MQ=255)
                                                                                                                                                                                                                                       cGCCTTCAGAATGGTACCAATCTCAACGTTATCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCAGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCAGGCAGTTGAGTCATTTACAGCAAACCCGACATGGTGGTCAATTGCGTGGACACATTTGCAAAATGCATTTCGAGCCCGCGCACGTTACTGGCTTGCATACAAAAATCAGCCGAAAACCCTTGCCGCCCTTGATGGGCTGGAccatcacaacga                               >  4:429200/1‑268 (MQ=255)
                                                                                                                                                                                                                                           ttCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACtt                                                                                                                                                          >  7:416313/1‑149 (MQ=255)
                                                                                                                                                                                                                                            tCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCCAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCTCCAGCGCACGTTACTGGCCTGCCACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCgaaga                          >  2:135047/1‑274 (MQ=255)
                                                                                                                                                                                                                                            tCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCACCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAgg                                                >  2:473840/1‑252 (MQ=255)
                                                                                                                                                                                                                                             cAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACttgt                                                                                                                                                        <  5:244794/149‑1 (MQ=255)
                                                                                                                                                                                                                                               gAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACttgttg                                                                                                                                                      <  6:230107/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    gTACCAATCTCAAAGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCAt                                                                                                                                                 <  8:404361/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCAtt                                                                                                                                                >  5:115853/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        cAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGAc                                                                                                                                                            >  8:459515/1‑134 (MQ=255)
                                                                                                                                                                                                                                                        cAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGAc                                                                                                                                                            <  7:459515/134‑1 (MQ=255)
                                                                                                                                                                                                                                                          aTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACACAATATCAGCCGTAAACCCTTGCAGTCCCTGCTAGGCCGGTTCATCAGTACGCAAAGAAATGACCGGgcgc             >  4:193639/1‑273 (MQ=255)
                                                                                                                                                                                                                                                               aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCa                                                                                                                                                                                                         >  2:28442/1‑82 (MQ=255)
                                                                                                                                                                                                                                                               aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCCCCGTTACGTTTGCCCTGCAGTGGAGTAATTTCCAGCAACCCCGACTTGTTGGCTCTCTGCGTGGCCCCTCCTGTAAAGTTCATTCCGCGCCAGCGCACGTTACTGGCCTGACACAAACTATCAGCCGTAAAGCCTTGACGTCCCTGAACGTCCGGTTCACAACTACGCGAAGAAATCACCGGACTCGGAAAc       >  4:357850/1‑274 (MQ=255)
                                                                                                                                                                                                                                                               aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCCACATCTGTAAAATTCATTCCGCGCCAGCGCCCGTTACTGGCCTGCCACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCGAAGAAATGCCCGGGCGCGGCCAc       >  1:53457/1‑274 (MQ=255)
                                                                                                                                                                                                                                                               aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCGAAGAAATGACCGGGCGCGGCAAc       >  2:293489/1‑274 (MQ=255)
                                                                                                                                                                                                                                                               aaCGTTTTCCAGCCGTGGCTGGAAGGTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGGAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATATGTAAAATTCATTCGGCGCCAGCGCACGTTACTGTCCTGCAACAAAATATCAGCCGTAAAGCGTTGCAGTCCCTGCTAGGCCGGTTCATCAATACGCGGAGAAATGACCGGGCGCGGCaaa       >  2:10655/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                  gTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACAt                                                                                                                                   >  8:435220/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   ttttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGt                                                                                                                                                                                                     <  4:28442/82‑1 (MQ=255)
                                                                                                                                                                                                                                                                   acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                                                                                                                                                                                                >  7:9806/3‑71 (MQ=39)
                                                                                                                                                                                                                                                                   acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                                                                                                                                                                                                <  8:9806/69‑1 (MQ=39)
                                                                                                                                                                                                                                                                    tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCGAAGAAATGACCGGGCGCGGCAACGTTGa  <  2:193641/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCCTACAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCATGCAGTTGAGTAATTTCCAGCAAACCCTACTTCTTGGTGCGTTGCGTCGCAACATCTGTAAACTTCCTTCCGCGCCAGCCCACGTTCCCTGCCTGCACCAAAATATCAGCCGAAAAGCCTTCGATTCCCTGATTTGCCGGTTCCGCACTACCAGAAGAAATGACCGGGCCCGGCCAcctacc  >  4:118980/1‑269 (MQ=255)
                                                                                                                                                                                                                                                                     ttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTg                                                                                                                                >  7:375136/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCa                                                                                                                                       >  4:419147/1‑141 (MQ=255)
                                                                                                                                                                                                                                                                          gCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACAt                                                                                                                                   <  2:419152/141‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                          
GTCAAGCCGCGTCACGTTATCAAAGTTTTCAGCGGCCTTCACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTCCAGCAAACCCGACTTGTTGGTCATTTGCGTGGCAACATCTGTAAAATTCATTCCGCGCCAGCGCACGTTACTGGCCTGCAACAAAATATCAGCCGTAAAGCCTTGCAGTCCCTGATAGGCCGGTTCATCAATACGCGAAGAAATGACCGGGCGCGGCAACGTTGA  >  NC_000913/2140131‑2140664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: