| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,590,767 | T→C | 5.3% | intergenic (+59/+40) | acrD → / ← ypfM | aminoglycoside/multidrug efflux system/stress‑induced small enterobacterial protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,590,767 | 0 | T | C | 5.3% | 312.5 / 5.5 | 94 | intergenic (+59/+40) | acrD/ypfM | aminoglycoside/multidrug efflux system/stress‑induced small enterobacterial protein |
| Reads supporting (aligned to +/- strand): ref base T (43/45); new base C (2/3); total (45/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-03 | |||||||||||
ACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACG > NC_000913/2590501‑2591038 | acaagacagtttgatgaCGTCGTTGGAATTTCTCTTAGGCATATGGAGAATGGATACAAGCACGAGTTCCGGTTCCGGTGTTCAGCATGCGGTGGTTACTGGCGTGCTGGGCGTGAGGATTTCGGCCACTATTCTGTCTATTTCCTTCGGGCCGCTGTCCT‑TTG‑TGCTAGTGCGCCGACGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGTCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCg < 4:150901/264‑1 (MQ=255) ttCGGATGAACTGGCGGGCATTTATCTTCGGCGTATTGACAATGGCAACCAGCACGGGTGCCGGTTCCTGTGGTCAGGATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt < 2:479082/274‑1 (MQ=255) cTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAATACCTGCTCAGCGGGTTCTTGCTGTTTGTAc > 2:472242/1‑275 (MQ=255) cTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCGGGTGCGCCGCACCTTCCCTCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGAAATTCCAATGTGTAGCCTTTTTCAACTTTCCGATTCAGAAACTGCTCAGCGGGTTCTTTCTTTTTGtca > 4:193210/1‑273 (MQ=255) cGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTGCAACTTTCCGATTAAGAACCTGCTc > 2:150902/1‑251 (MQ=255) gCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGCTTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTTGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGTCGACATTCCAATGGATCGCCTTTTTCAACTTTCCGATTTAGAACCTGCTCCACTGTTTTTTGCTGTTTgctct > 4:315779/1‑269 (MQ=255) cGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt > 2:342350/1‑243 (MQ=255) cGTATTGCCAATGTGAAGCAGCACGGGTGCCGGTTCCGGGGGGCAGCAGGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGGTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttactta < 1:150649/274‑1 (MQ=255) cGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttactta < 2:315783/274‑1 (MQ=255) cgaagggCAACCAGTACGGGGGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTCCTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTCTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCAt < 2:104614/270‑1 (MQ=255) aTGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TTCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTCCTTACGAAGCATaca > 2:58730/1‑273 (MQ=255) ccAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCCGTTTGTACTTCGTCCCAGGAATTACTTACGAAGCAAAACTTCGAt > 2:110999/1‑274 (MQ=255) tGCCGGTTCCTGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTc < 2:278257/274‑1 (MQ=255) gttcggCATGGGGTGGGTAATGGGGTAATGTGGTGGATGCTTTCGGCCACAATCTTGGCTAGTTGCTTCGTTCGTCTGTTCT‑TTG‑TGCGGGTGCGCCGCCCCTTCCCGCTAAAGCCGGGCCCTGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTGGCCTTTTTCAACTTTCCGATTAAGAACCTGGTCAGCCAGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGtt < 4:110998/269‑1 (MQ=255) ggtCAGCATGCGGGGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGtt < 1:147810/274‑1 (MQ=255) tCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAGAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCGGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCa < 2:211203/274‑1 (MQ=255) tggtgtatctggCGGGATGGTTTCGGGCACAGTTCGGGCAATTTACTTCGTGCGGCTGGTCT‑TTG‑TGGTGCGGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTGTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACct < 4:180415/265‑1 (MQ=255) ggctggaTGGGGGGGAGTATTTCGGCCACTATTCCGGGTATTTACGTCGTTGCGCTGTGCA‑TTT‑TGCTGGGGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTGCCGATTAAGAGCCTGCTCAGCGGGTTCTTGCTGTTTGTACATTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACct < 4:147359/268‑1 (MQ=255) ggCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac < 2:193212/272‑1 (MQ=255) cGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACctctc > 2:147360/1‑275 (MQ=255) aTGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATATAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGCACCTCCTCAGCGGGTTTCTGCTGTTTGTACTTTTCATCAGGAATTACTTCCGAATCATAACTTCGCTCAATCCTTTCAATTTCCCCCGTTCACTTTTCATCATAACAACCTCTCtttc > 4:43681/1‑273 (MQ=255) gCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTCTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACg < 2:111088/247‑1 (MQ=255) cGGGATGATTTCGGCCACTATTCTGGCTATTTACTTTGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGACTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGCTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCAAGTTAACGTTCAACCATAACAACCTTCCTTATAATTa > 2:494785/1‑275 (MQ=255) gggcaagggCTATTTCCTTCGTG‑CGCTTTTTTGTTG‑TGCTGGTGCGACGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGaa < 4:105672/267‑1 (MQ=255) ttCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCCGCTGAGCAGGTTc < 7:251589/149‑1 (MQ=255) cTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt < 6:114985/149‑1 (MQ=255) cTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt < 8:168760/149‑1 (MQ=255) ttACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGt > 2:78912/1‑137 (MQ=255) ttACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCCGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCACTTCCCCAGTTCACGTTCAATCAtca > 1:115103/1‑225 (MQ=255) ttACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCgtgt > 1:3672/1‑274 (MQ=255) ttACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCgt > 2:42676/1‑273 (MQ=255) ttCGTGCCGCTGTTCT‑TTGTTGCT‑GTGCGCCTCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGaa < 4:42676/275‑1 (MQ=255) ttCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt < 5:31774/134‑1 (MQ=255) ttCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGtt > 6:31774/1‑134 (MQ=255) ttCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCtt < 4:78911/137‑1 (MQ=255) ttCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACt > 5:454526/1‑149 (MQ=255) tCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAACAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTAc > 2:105673/1‑252 (MQ=255) cGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACttt < 5:267703/149‑1 (MQ=255) cGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTCTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACttt < 7:10396/149‑1 (MQ=255) cT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAtt > 8:103382/1‑149 (MQ=255) t‑ttG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAtta < 6:454526/149‑1 (MQ=255) tg‑tgCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATtg > 4:153011/1‑275 (MQ=255) cTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATtg < 2:153013/271‑1 (MQ=255) gTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAg > 5:470077/1‑149 (MQ=255) gTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAata > 4:13220/1‑239 (MQ=255) gcgcCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCa < 6:296803/149‑1 (MQ=255) cTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGa < 6:470077/149‑1 (MQ=255) ttCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGAt > 7:69980/1‑149 (MQ=255) ccGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATaaa < 7:120047/149‑1 (MQ=255) aaGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaa > 3:102053/1‑178 (MQ=255) aGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTc < 7:277830/149‑1 (MQ=255) gCCGCGCCCGGAATAAGCAATAAAAAACGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTCTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTcc > 7:269275/1‑149 (MQ=255) gcacgcTCGGGAGAAGCAATAAAGATGGCGACCTCGCAATGTGTAGTGTTATTCAACTTGTCGATTAAGAATAGGTGATGCAGATTATTGCTGTTTTTACTTTGGATCACTACTTTGTAACGAAGAATAACTTCGATACAGTCCGTCCAGTTACCCAGTTCAAGTTCAATCATAACAAGCTAGCTTATCATTATGGGTATGCTACGCAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAATTCt < 4:108973/271‑1 (MQ=255) cgcgCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac < 1:102053/178‑1 (MQ=255) tAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaa > 2:113900/1‑156 (MQ=255) aaaaGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCAc > 7:473358/1‑141 (MQ=255) aaaaGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCAc < 8:473358/141‑1 (MQ=255) aaaggtcccATGCAATTGTTTNNCTTTTNTCAACTTTCCGATTAAGAACCTGCTCAGAGGGTTCTGGCTGTTTTTACTTTGTATCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCg < 3:3672/266‑1 (MQ=255) aaaGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac < 4:113899/156‑1 (MQ=255) tGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACctc < 6:171975/149‑1 (MQ=255) tgctcccctttCAACTGTCCGGTTAAGACCCTGCTCGTCGGGTCGTTGCTGTTTGTACCTGTTGTCAGGGATTTCTGACGAAGCATAACTTCTATAAAGGCTGTCCAGTTTCCCACTTTACGTTCAATCATAACAACCTCTCTTACAAGTATGGGTATTCTACGGAAACCATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCGTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATa < 4:155506/267‑1 (MQ=255) tCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaa > 1:110714/1‑132 (MQ=255) cGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTCGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCa > 1:77573/1‑118 (MQ=255) gCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTAt > 7:220930/1‑149 (MQ=255) ccTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaa > 8:175587/1‑132 (MQ=255) ccTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaa < 7:175587/132‑1 (MQ=255) cTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac < 3:110714/132‑1 (MQ=255) tttttCAACTTTCCGACTAAGAACCTGCTCCGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGTAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGtt < 3:77573/118‑1 (MQ=255) tttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCaa > 2:240186/1‑275 (MQ=255) ttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttact > 2:336145/1‑67 (MQ=255) ttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTaa > 2:520394/1‑181 (MQ=255) aaCTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCt > 8:83556/1‑149 (MQ=255) aCTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACg < 4:336141/67‑1 (MQ=255) aCTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTa < 4:520389/181‑1 (MQ=255) tttCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACg > 7:325857/1‑149 (MQ=255) ttCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCggg > 2:74919/1‑206 (MQ=255) tCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACAGTGGTGAAGCTAATTTACTc > 4:522918/1‑180 (MQ=255) cGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGaaa > 8:256515/1‑149 (MQ=255) gATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAAc < 8:69980/149‑1 (MQ=255) gATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGAc < 4:74918/206‑1 (MQ=255) aTTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGAtt < 2:522923/180‑1 (MQ=255) ttAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACaa < 7:77802/149‑1 (MQ=255) ttAAGAACCTGCTCAGCGGGGTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTgatgat < 2:21547/241‑1 (MQ=255) aaGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAata > 6:74372/1‑149 (MQ=255) aaGAACCCGCTGAGCAGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACg < 7:68546/140‑1 (MQ=255) aaGAACCCGCTGAGCAGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACg > 6:25823/1‑140 (MQ=255) aaGAACCCGCTGAGCAGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACg < 5:25823/140‑1 (MQ=255) aaGAACCCGCTGAGCAGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACg > 8:68546/1‑140 (MQ=255) aGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttacttac > 6:475224/1‑54 (MQ=255) aGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttacttac < 5:475224/54‑1 (MQ=255) gAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAAtata > 5:274736/1‑149 (MQ=255) aaCCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATAc > 8:298470/1‑149 (MQ=255) aCCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATAcc > 5:92861/1‑149 (MQ=255) aCCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACg < 2:43681/275‑1 (MQ=255) tGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCgtg < 7:103382/149‑1 (MQ=255) | ACGCCCGATTCTGATGACCTCGCTGGCATTTATCTTCGGCGTATTGCCAATGGCAACCAGCACGGGTGCCGGTTCCGGTGGTCAGCATGCGGTGGGTACTGGCGTAATGGGCGGGATGATTTCGGCCACTATTCTGGCTATTTACTTCGTGCCGCTGTTCT‑TTG‑TGCTGGTGCGCCGCCGCTTCCCGCTGAAGCCGCGCCCGGAATAAGCAATAAAAAAGGCGACATGCCAATGTGTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTATAATTATGGGTATTCTACGGAAACAATATACCGTGGTGAAGCTAATTTACTCGATTGCTGCGATGACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACG > NC_000913/2590501‑2591038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |