Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,761,623 | C→A | 5.6% | V640L (GTA→TTA) | yfjK ← | radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,761,623 | 0 | C | A | 5.6% | 492.5 / 13.5 | 142 | V640L (GTA→TTA) | yfjK | radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage |
Reads supporting (aligned to +/- strand): ref base C (64/70); new base A (4/4); total (68/74) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01 |
Only 100 of 143 total aligned reads displayed.
AATGTCGTCTAATACCGAGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTC > NC_000913/2761353‑2761892 | tGTCGTCTAATACCGAGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTTCAAATGTCCTGAGTATCCCGAAGATACTGGCCCAACTCATCCACATCAGCCTAGTAGGAACATTCCAGCAAGTCTACCAGTCGGCGTAACGTCTGAATGGGAATCCCAATCTCTTCTAAGCCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑ATTTACAGCgt > 1:101121/1‑274 (MQ=255) tGTCGTCTAATACCGAGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCACACGCCAATTTGACATTTGTGTAATCGACTTTGCTGCGTATACCACA‑CTTTACAGCgt > 1:108698/1‑274 (MQ=255) aGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTACGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtc > 4:144384/1‑275 (MQ=255) aGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTCCAAATGACCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCAAGCAAGTCTACCAGTCGGTGTAACGTCAGAATGGGAATGCCAATCTCGTAGAAGCCATTGACACTTGGTGGTCGATGAAACCTTTCGAACTCCAATTTCACATTAGTGTACTGCACTTTGCTGCGTATACCACG‑CTTTCCAGAGGGGGGTTTAATTACAtc > 4:388565/1‑275 (MQ=255) tACCCAATCGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGt > 2:203945/1‑275 (MQ=255) cGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa < 2:144385/275‑1 (MQ=255) tACTCCAAATCGACGGAGTACCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAATTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTTTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTGGGGTATAGCCGAAGGTa < 4:394842/275‑1 (MQ=255) cTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCCAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCt > 2:207502/1‑275 (MQ=255) ttacccAAAGTTAGTGGGTCAAATCAACCACATCTACCTCGTGGGAAAAGTCAAGCAATTTTACCAGTCGGTGTAACGGCGTAAGGGGAATGCAGAACTCTCCTAATGTATTGACAACGTGTGGTAGATGGAAGCTTTCGAACGCCAAGTTGACAGGTGTGTAATCGACGTTGCTTCGTATACCACG‑ATGTACAGCGTGGTGTTTAACTACATCCTCCAGCCAGCTGAGCACCTTAGGGAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCaaa < 4:207499/272‑1 (MQ=255) aaGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt < 2:91186/232‑1 (MQ=255) aggtACTGGCTCAACGCAGCCAAATAAGCCTCGTCGGAAAATTCCAGACAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt < 4:395562/272‑1 (MQ=255) aGATACTGGCCTAACTCATCCACATCAGCCTCGTCGGAAAATTCCCGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATTCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt < 2:113264/275‑1 (MQ=255) tGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGc > 2:493400/1‑275 (MQ=255) ggCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGc < 2:388570/274‑1 (MQ=255) tCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag > 4:106432/1‑215 (MQ=255) caTCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGta < 2:106433/215‑1 (MQ=255) aTCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt > 4:260984/1‑275 (MQ=255) cctaggcgGCAAAATCCCGCAAGTCTACCAGTCGGTGTAACGTCGGAATGGGAATTGCAATCCCTTCTAAGGCATTGACACCCGGTAGTAGATGGAAACTTTGGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTGTACCACG‑CTTTACAGCGAGGTGTTTAACTACATCCTCCGTCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtct < 4:203942/267‑1 (MQ=255) tcgtcgGCAAAGTCCCTCAAGTCGACCTGTCGGTGTAACGTCTGAATGGTAATGCCAATCTCTTCTAAGTCATTGATACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGATCACCTTAGGTAGGGTATAGCCGAATGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc < 4:493395/275‑1 (MQ=255) tcgGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAg > 1:154402/1‑183 (MQ=255) tcgGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta > 2:79532/1‑195 (MQ=255) aaaaTTCCAGAAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta < 4:79531/195‑1 (MQ=255) aTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCg > 5:102537/1‑149 (MQ=255) ttCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCgt < 7:227363/149‑1 (MQ=255) tCCAGCAAGTCTACCAGGTGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACAACTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACAGGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCa < 2:331914/275‑1 (MQ=255) ccAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCa < 2:363387/274‑1 (MQ=255) ccAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTGTAAAGCgtgg < 7:455574/149‑1 (MQ=255) aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCgtggtg > 6:231554/1‑149 (MQ=255) aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCgtggtg > 8:365446/1‑149 (MQ=255) aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTGTAAAGCgtggta < 6:455781/149‑2 (MQ=255) ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTGTACAGCg < 1:103190/134‑1 (MQ=255) gTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAATGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtcc < 8:287372/149‑1 (MQ=255) gTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtcc < 5:18820/149‑1 (MQ=255) ggTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtcctcc < 6:1529/149‑1 (MQ=255) ggTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtcctcc < 8:380045/149‑1 (MQ=255) gtAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGttt < 6:396262/133‑1 (MQ=255) tAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGGGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa < 4:484255/155‑1 (MQ=255) gTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtcc < 7:307585/138‑1 (MQ=255) cTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa < 5:432063/149‑1 (MQ=255) aaTGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAg > 2:371020/1‑136 (MQ=255) ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa < 4:371015/136‑1 (MQ=255) ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag < 8:459013/149‑1 (MQ=255) ccAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag < 8:218740/149‑1 (MQ=255) cAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAc > 6:335543/1‑139 (MQ=255) cAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAc < 5:335543/139‑1 (MQ=255) cAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg < 7:57332/149‑1 (MQ=255) ctctTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAATCTNAGCACCTTNGGTAGGGTATAGCCGAAGGTCTTGGTTAtaa > 3:30030/1‑168 (MQ=255) ttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGc > 6:203506/1‑149 (MQ=255) ttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGc < 6:101575/149‑1 (MQ=255) aaGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa > 7:386045/1‑149 (MQ=255) aGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg < 7:365446/149‑1 (MQ=255) ggCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa < 1:21806/167‑1 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGttt < 5:177543/98‑1 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGttt > 6:177543/1‑98 (MQ=255) ttGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTa > 4:391221/1‑100 (MQ=255) ttGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGNCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGAAATGGAAAGAGTACGTTTCTCCACTTCAGAAATCNCCGGTGGTGCATAATCGATCTGATTTTTCANNTATTCCGAATAACTTTCAGCCATTaaaaa > 3:66845/1‑273 (MQ=255) tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTg < 8:242693/149‑1 (MQ=255) tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTg < 8:386045/149‑1 (MQ=255) cacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACAtc < 2:391226/100‑1 (MQ=255) cacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGtt < 6:461850/149‑1 (MQ=255) cacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTa < 2:505792/188‑1 (MQ=255) cTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg > 7:164335/1‑149 (MQ=255) tAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa > 6:140926/1‑149 (MQ=255) gAAACTTTCGAGCGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt > 7:382337/1‑149 (MQ=255) gtataagggaaataaTTTGACATGTGTGTAATTGACTTTGCTTCGTATACCACC‑CGTTACAGCTGGTTGTTTACCTACCTCCTCCATCCAGCTGGGCACCTTAGGTAGGGTATAGCCGAAGGGATTGCTTATGAGCTTCAAAGCATTGTTGAGGTCAAGGTAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCAGATTCGATCTGATTTTTCAGATATTCCTAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGa < 4:243561/262‑1 (MQ=255) cTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt < 8:164335/149‑1 (MQ=255) ttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGa < 5:55641/149‑1 (MQ=255) ttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGa < 5:231554/149‑1 (MQ=255) cGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATcc < 1:66845/275‑1 (MQ=255) aagagacagttaCATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTTTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGt < 2:54757/263‑1 (MQ=255) cGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCaa < 6:406084/149‑1 (MQ=255) tccAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAAt > 8:152928/2‑149 (MQ=255) gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGGGTTTAACTACATCCTCCATCAAGCTGTGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATGGTTGAGGGCAAt > 5:380163/1‑149 (MQ=255) aaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCtt < 2:260988/275‑1 (MQ=255) aTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCt > 4:459891/1‑87 (MQ=255) aTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaa > 8:449888/1‑149 (MQ=255) gACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtagg < 2:459896/87‑1 (MQ=255) cATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTa < 6:14575/149‑1 (MQ=255) tgtAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt > 7:198446/1‑120 (MQ=255) ttAATCGCCGTTACTCCGGATACAACGCCTTTAC‑GCGATGGGTTTAACTACATCCTCCATCAAACTGCGCACCTAAGGGAGGGTATAGCCGAAGGTATTGGTGATGATCATCAAATCAGTGTTGAGGGGAATGGAAAGAATACGTGTGTCCCCTTCAGAAATCCACTGACGTGCATAATCGGTCTGATTTTTCAGATATTCCGAATTACTTTCATCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTGGGCTTAACAGGGACCCCAt < 4:245159/274‑1 (MQ=255) tAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAATGTCGTGCATAATCGATCTGATTTTTCAACTATTACGAATAACTTTCAGCACTTAAATAGCCGGCCAGTTTTTCGATCATGATATCCGTAGGCTTAACaccgggcttt > 4:54756/1‑264 (MQ=255) aTCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGt < 5:297471/142‑1 (MQ=255) aTCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGt > 6:297471/1‑142 (MQ=255) tCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAc > 8:347205/1‑68 (MQ=255) tCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcccc > 5:113343/1‑149 (MQ=255) tttGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt > 5:60007/1‑114 (MQ=255) tgctgcGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg > 1:70362/1‑83 (MQ=255) ctgcGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCt < 4:189004/61‑1 (MQ=255) ttaaacaCCACG‑CTGTAAAGCGTGGTATTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATcc > 7:177356/7‑149 (MQ=255) tataCCACG‑CTTTACAGCGTGGTGTTTGACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGttt > 2:253969/1‑219 (MQ=255) caCCACG‑CTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGa > 6:70404/2‑87 (MQ=255) aCCACG‑CTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt > 6:254124/1‑98 (MQ=255) aCCACG‑CTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt < 5:254124/98‑1 (MQ=255) aCCACG‑CTGTAAAGCGTGGAGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTc > 8:109199/1‑149 (MQ=255) cACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAGCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGGGCTTAAAATCATTGTTGAGGGCAATGGAAAGAGGACGTTTCTCCCCTTCAGAATTCCACTGTCGt < 6:380163/149‑1 (MQ=255) cACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt > 7:370320/1‑149 (MQ=255) aCG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTg > 6:268697/1‑149 (MQ=255) aCG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTg > 6:96614/1‑149 (MQ=255) tttACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa > 4:274261/1‑65 (MQ=255) tACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTc < 2:415876/272‑1 (MQ=255) | AATGTCGTCTAATACCGAGCGCGCGCCTGATAAACATCTGATCAACGTACCCAATCGATCTACTCCAAATGTCCTGAGTATCCCGAAGATACTGGCTCAACTCATCCACATCAGCCTCGTCGGAAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACG‑CTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCATAATCGATCTGATTTTTCAGATATTCCGAATAACTTTCAGCACTTAAATAGCCGGCAAGTTTTGCGATCATGACATCCGTAGGCTTAACAGGGACCCCATTGCGACTGAACGTATGGGGAACCAGTC > NC_000913/2761353‑2761892 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |