Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,778,123 | G→T | 11.0% | intergenic (+341/+23) | ypjF → / ← ypjA | CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,778,123 | 0 | G | T | 11.0% | 319.4 / 23.4 | 101 | intergenic (+341/+23) | ypjF/ypjA | CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter |
Reads supporting (aligned to +/- strand): ref base G (47/42); new base T (4/7); total (52/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.75e-01 |
Only 100 of 103 total aligned reads displayed.
TCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTCAACTTCCATACCGTTC > NC_000913/2777861‑2778395 | tccccCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGGGACCAAAAAGGATACGCAAAGGAGCCGCGGCCCCTGGTA‑AAAGGAAAGCCCACAgccttcacttat > 4:285314/1‑263 (MQ=255) aCATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTCAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGCCATAAAAGGATACGCACAGGAGCCGCCGCTCCGGGTA‑ACTTGAAAGCCCAGAGCCGGGGCCTTTTTCGTTGATGTTAAGaa > 4:78602/1‑274 (MQ=255) ttttCTTTTAGGAACAACAATAGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGAc < 2:149997/275‑1 (MQ=255) ggACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGt > 2:128818/1‑275 (MQ=255) aaaaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCAc < 2:79820/275‑1 (MQ=255) aaTGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGtta < 2:280344/252‑2 (MQ=255) aaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGt > 2:20513/1‑232 (MQ=255) aaaTTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCCCACCAGCTACCGAGTTCCACGGGGAtt > 2:141558/1‑275 (MQ=255) ttGAACTGAAGATTACAATCAATTTAACAACAAGTGACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTc < 2:78603/273‑1 (MQ=255) aaatcaaaaTTACAACACATTTAACAACAAGGTACACAACCAATGCAGGCTCCGCCAGCCCACCAGTCATGATTGGACGGGGTAAGGCCAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCacac < 4:327373/267‑1 (MQ=255) aaGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAACCACCGACCAGACGGTGAGGAGCCATAAAAGGAAACGCAAAGGAGCCGCGGCTCCTGGTA‑ACAGGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGCCCAGTTCAAACCAGCAACCGCGTTCCACGAGGCTTCCAAACCgcc > 4:31695/1‑272 (MQ=255) aagtACAAACAAGTTAACAAAAAGTTAAACAAACAATTCAGACTCCGCCAGCCAACCAATCATGATTGGACGGTGTAAGGACAACACAAACAAAAACAGGAAGTGAGAAGTCACAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACt < 4:128817/272‑1 (MQ=39) tcaatTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACCTAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCCGCTACCGCGTTCCACGGGGATTCCACACCGGCCCTATGGCtat > 1:48238/1‑275 (MQ=255) tttAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACAAAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACgg < 2:190594/245‑1 (MQ=255) acaACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCg > 1:149038/1‑275 (MQ=255) aaCCAATTCAGACACCGCCAGCCCCCCAAGCAGGATTCGACGGGGTAAGGACAACACCAACAAAAACAGGAAGGTAGAAGTCTCAGCAAAACACCGACCAGACGGGGAGGGGACATAAGAGGATGCGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACGGATGTGAGCTTTTGCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCt < 2:52053/275‑1 (MQ=37) gACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTActg > 2:440604/1‑275 (MQ=255) gCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGCCGGTGAGGAGACATACAAGGATACGCAAAGGAGTCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGAGCTTTTTCGTTGACGGTAAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCCCCTACCGTACTGCTGAAt > 1:98044/1‑275 (MQ=255) cAGCCCACCAAACATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGa < 2:238342/275‑1 (MQ=255) cAGACCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGa < 2:31695/275‑1 (MQ=255) aGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGAt < 7:431009/149‑1 (MQ=255) gCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGAtg > 8:268276/1‑149 (MQ=255) ccaccaATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACATCtgtg > 5:386656/1‑149 (MQ=255) caATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCt > 6:94925/1‑149 (MQ=255) tCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACATCTGTGGGCtttc < 8:253147/149‑2 (MQ=255) tCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACATCTGTGGGCtttc < 6:386656/149‑2 (MQ=255) atttgactctgtAAGGCCAACACCAAAAAAAACAGTAAGTCAGCAGTCCCTGACAACCCACGACCATCCGGGGAGGAGCAATAAAAGGATACGCAAATGGGCCGCGGCTCCTGGTACACA‑GAACGCCCACAGATGTGTGCTTTTTCGTGTATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGCTGCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCCTTAATAcctgcc < 4:141557/266‑1 (MQ=255) gACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTgg > 2:283545/1‑274 (MQ=255) aCGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCCTAACGTACTGCTGACTGAGGTTTTAATCCCGGCCTGGt > 1:148322/1‑274 (MQ=255) tgtAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTGTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTcc > 4:334476/1‑191 (MQ=255) tgtAAGGACAACACCAACAAAAACAGGAAGTTAGAAGCCTCAGCAAAACACCGACCAGACGGCGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCAACAGATGTGGGCTTTTTCGTTGATGGTCAGAATGACCAGTTCACACCAGTTACCGCGTCCCACGGGGTGTCAAAACCCNCACTATGCCTCTACCCCACCCCAAGCTGCACCCCTACCCTACTTACGAATGAGGCCTCAATActcacccggcatat > 3:123221/1‑261 (MQ=255) tAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGaa > 5:372747/1‑149 (MQ=255) aGGACAACACCAACAAAAACAGGAAGTTAGAAGTGTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCacac < 2:334480/191‑1 (MQ=255) gACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGAc < 7:268276/149‑1 (MQ=255) acaacaCCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGAcc < 6:372747/149‑1 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGCGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCCCTATGCCNATACCCACACCCAAGATGCCCGCTTCACGCACTGCCCAATGCTGCTTTACTCCCCGCCTGATAAATTccctgtttat > 3:118892/1‑267 (MQ=37) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCCAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAAACGTGGGCTTTTTCGTTCATGGTCAGAACGACCAGTTCACAACAGCTACCGCGTTCCACGGGGATTCCCAACCGCCACTATGGCTAACCCCCACCCCAAGATCCCCGCGTACTGTCCCGCTTCATCAGACCTTAATACCTCCCTTGTAAATccctcctacac > 4:493416/1‑267 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCa > 4:394715/1‑157 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGc > 4:95134/1‑159 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCg > 4:289985/1‑187 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGa > 2:461249/1‑236 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGa > 1:99404/1‑236 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGc > 2:153410/1‑275 (MQ=255) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTACTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCAACACCGGCTCTATGCCTATACCCCACCCCAAGATGCCCGCTTAACGTACTCCTGAAAGAGGCTTTAATACCTGCCTGGAATATTcacaccctgc > 4:399750/1‑266 (MQ=37) acacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGAACAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGACGCGGCTCCTTGTA‑ACATGCAAGCACACAGATGTGGGCTTTTTCAGTGATGGTCAGAAGGACCAGTTCACACCTGTTACCGCGTTCCACAGGGATTATACACCGGAGTTATGACTATAAACCACACAACGAGACGCGATCAACGTACTGCGGAAGAAGGCTTTTAGACCCGCCTGGAATATGCCAcccttgc > 4:224936/1‑269 (MQ=255) cacCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGtt < 5:94925/149‑1 (MQ=255) acCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTc < 8:445990/149‑1 (MQ=255) caacaaAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTa < 2:394720/157‑1 (MQ=255) caacaaAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCAc < 2:289989/187‑1 (MQ=255) caacaaAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGa < 4:461244/236‑1 (MQ=255) aaaaaCAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCa > 7:47884/1‑149 (MQ=255) aCAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACt < 4:122677/180‑1 (MQ=255) aCAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACt > 2:122678/1‑180 (MQ=255) ttAGAAATCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTc > 8:288407/1‑149 (MQ=255) aGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCa > 8:405127/1‑149 (MQ=255) aGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTc > 6:331563/1‑149 (MQ=255) aGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCa < 2:399755/273‑1 (MQ=255) gCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTcc > 8:44440/1‑149 (MQ=255) cGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCa < 7:44440/149‑1 (MQ=255) gACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCAc > 5:259873/1‑149 (MQ=255) aaacacAAAAGGATACGAAAAGGGGCCGCGGGTCCTGGTA‑ACATGAAAGCCCACAGATGTGTGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGa < 4:283541/273‑1 (MQ=255) aCATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATaccccacc < 7:405127/149‑1 (MQ=255) aTAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATaccccacccc > 8:262169/1‑149 (MQ=255) tAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCa > 4:362972/1‑95 (MQ=255) aaGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCAACCCAAGa < 6:259873/149‑1 (MQ=255) aGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGAtgt > 7:57337/1‑51 (MQ=255) aGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGAtgt < 8:57337/51‑1 (MQ=255) aGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTa < 2:362977/95‑1 (MQ=255) actcAAAGGAGCCGCGGCTCCTCGTA‑ACATAAAAGCCCACAGATGTGGACTTTTTCGTCGATGGTCAGAACGACTAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTCTACCCTACTCTAAGACGCCCGCTTAACTTACTGCTGAATGAGGCTTTAATACCCGCCTGGTACCTTCAACGTCTGCCCGACAAATAATTGACCATATTACCTTCACTATCCCCTTTCACCCGGGTCTCCACGACAAACTtttttcc > 4:400382/4‑271 (MQ=18) aCGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACgggg > 4:535191/1‑105 (MQ=255) aCGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATAcctgcc > 2:393567/1‑185 (MQ=255) gCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCt < 8:47884/149‑1 (MQ=255) gCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCt > 8:338533/1‑149 (MQ=255) aaaGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTc < 2:535196/105‑1 (MQ=255) aaaGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGt < 4:393562/185‑1 (MQ=255) ggAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACg > 7:318449/1‑149 (MQ=255) ccGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTTACGAAATTACCGTCACTATTCACTTTCACCCGGtt > 2:24562/1‑242 (MQ=255) cgGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCca < 7:156417/97‑1 (MQ=255) cgGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCca > 8:156417/1‑97 (MQ=255) cgGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCa > 2:293071/1‑274 (MQ=255) cgGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCa > 2:347211/1‑274 (MQ=255) tGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAg > 5:53164/1‑149 (MQ=255) ggTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAgg < 6:53164/149‑1 (MQ=255) gTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGc < 7:338533/149‑1 (MQ=255) tA‑ACATGAAAGCCTGCAGTGGTGGGCTTTTTCGTGGATGGTCAGACCGACCAGTTAACACCAGCTACCGCGTTCCACGGGGATTCCACCCCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGTTTTAATACCTTCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTc < 4:440599/274‑1 (MQ=17) tA‑ACATGAAAGCCCACAGATGTGGTCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTc < 1:123221/274‑1 (MQ=255) tA‑ACATGAAAGCCCACAGATGTGGGCTTTTTAGTTGATGGTCAGAACGACCAGTTCACCCCAGCTACCGCGTTCCACGGGGATTCCACACCGTCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTc < 2:493421/274‑1 (MQ=255) ggggcaggAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTc < 1:114873/268‑1 (MQ=255) a‑aCATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCtt > 5:301535/1‑149 (MQ=255) gaaaaaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTa < 4:321376/91‑1 (MQ=255) gAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCt < 8:424002/143‑1 (MQ=255) gAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCt > 7:424002/1‑143 (MQ=255) gAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATa < 6:301535/149‑1 (MQ=255) aaaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGaa > 5:111391/1‑135 (MQ=255) aaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACcgc < 7:331791/62‑1 (MQ=18) aaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACcgc > 8:331791/1‑62 (MQ=255) aaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGc < 5:227030/94‑1 (MQ=255) aaGCCCACATCTGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGc > 6:227030/1‑94 (MQ=255) aGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGt < 7:288407/149‑1 (MQ=255) gATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCGTTAATACCTGCCTGGCATAGTCCCACTCTGCCCGACAAATAATTTCAGAAACTACCGTCAATCTTNACTTTCACCCGGTTCTCATCGACAAATTCTTTTCGCACATCCTCCCTCTGCCCCGTCTCAATCTCCacacttgtc > 3:114873/1‑268 (MQ=14) | TCCCCCGAAAAAACCTGACATTTTCTTTTAGGACCAACAATGGGACCAAAATGAAAATTGAACTGAAGATTACAATCAATTTAACAACAAGTTACACAACCAATTCAGACTCCGCCAGCCCACCAATCATGATTGGACGGTGTAAGGACAACACCAACAAAAACAGGAAGTTAGAAGTCTCAGCAAAACACCGACCAGACGGTGAGGAGACATAAAAGGATACGCAAAGGAGCCGCGGCTCCTGGTA‑ACATGAAAGCCCACAGATGTGGGCTTTTTCGTTGATGGTCAGAACGACCAGTTCACACCAGCTACCGCGTTCCACGGGGATTCCACACCGGCACTATGGCTATACCCCACCCCAAGATGCCCGCTTAACGTACTGCTGAATGAGGCTTTAATACCTGCCTGGTATATTCCACGTCTGCCCGACAAATAATTGACGAAATTACCGTCACTATTCACTTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCACGGCTCAACTTCCATACCGTTC > NC_000913/2777861‑2778395 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |