Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,838,222 A→T 6.5% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,2220AT 6.5% 261.4 / 5.5 75intergenic (‑117/+32)hydN/ascGformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base T (2/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGC  >  NC_000913/2837962‑2838477
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                               
tttAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTTTTATTTAATGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACTATg                                                                                                                                                                                                                                                   >  1:62657/1‑275 (MQ=255)
                  cgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGtt                                                                                                                                                                                                                                 <  2:208522/275‑1 (MQ=255)
                               tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTAACGATGACTTGTTATGACAATGTTTATGGTGAACGa                                                                                                                                                                                                                     >  2:30238/1‑274 (MQ=255)
                                                    tCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTCATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGCTGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGa                                                                                                                                                                                               >  2:451862/1‑275 (MQ=255)
                                                                                acgcAACTTAAAAGGTACGGGAACAAAGACATTTTCTAGCCTCAGGAATGATGAAACCGTTCATCAAATTCTCCAGCAAGGACAGGTAATGCGTCGATTCATTCACAAATCATGCCAGTTTTTAATTTCCTTGTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAGGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGTTCAGTTTTCCGCTGAAGGTTTTcggcg                                                                                                                                                                    <  4:451857/274‑1 (MQ=255)
                                                                                                                            gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGa                                                                                                                                                                                                                                                     <  8:124773/149‑1 (MQ=255)
                                                                                                                                    gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATg                                                                                                                                                                                                                                                   >  7:26866/1‑143 (MQ=255)
                                                                                                                                    gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATg                                                                                                                                                                                                                                                   <  8:26866/143‑1 (MQ=255)
                                                                                                                                    gaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgt                                                                                                                                                                                                                                             <  7:339840/149‑1 (MQ=255)
                                                                                                                                     aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtcgt                                                                                                                                                                                                                                            <  8:127103/149‑4 (MQ=255)
                                                                                                                                     aaaCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAtgtg                                                                                                                                                                                                                                            <  6:88964/149‑1 (MQ=255)
                                                                                                                                            tcatcaAATTCTCCAGCAATGACAGTTGATGCGCAGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTGAGGAAATTAATTTATGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACaa                                                                                                                                                                                                                                     <  7:358424/149‑1 (MQ=255)
                                                                                                                                            tcatcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACaa                                                                                                                                                                                                                                     <  5:277827/149‑1 (MQ=255)
                                                                                                                                            tcatcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaaaa                                                                                                                                      >  1:96594/1‑244 (MQ=255)
                                                                                                                                             catcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAAt                                                                                                                                                                                                                                    <  5:345078/149‑1 (MQ=255)
                                                                                                                                             catcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTcc                                                                                                                  >  2:315262/1‑263 (MQ=255)
                                                                                                                                              atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATg                                                                                                                                                                                                                                   >  7:359769/1‑149 (MQ=255)
                                                                                                                                               tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGt                                                                                                                                                                                                                                  <  5:35049/149‑1 (MQ=255)
                                                                                                                                                 aaaTTCTCCAGAAATGAAAGTTAATGGGCCGGTACATTCAAAAATCATGCCAATTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGAAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGaa                                                                                                              <  4:315258/263‑1 (MQ=255)
                                                                                                                                                  aaTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTa                                                                                                                                                                                                                               >  6:100276/1‑149 (MQ=255)
                                                                                                                                                   aTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTAt                                                                                                                                                                                                                              <  7:73022/149‑1 (MQ=255)
                                                                                                                                                          aGCAATTACAGTTAATGCGCATATACAATCACAAATCATGCCAGTTTTTAATTTAATGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTATAGACGATTACATGTGATGAAAATGTTTATCGCGAAg                                                                                                                                                                                                                       <  8:436107/149‑1 (MQ=255)
                                                                                                                                                              aTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                                                                                                                                   <  8:282732/149‑1 (MQ=255)
                                                                                                                                                              aTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                                                                                                                                   >  8:34879/1‑149 (MQ=255)
                                                                                                                                                                 aCAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAt                                                                                                                                                                                                                <  5:100276/149‑1 (MQ=255)
                                                                                                                                                                   aGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                                                                                                                              <  8:236170/149‑1 (MQ=255)
                                                                                                                                                                                    aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtga                                                                                                                                                                                                          <  8:55310/136‑1 (MQ=255)
                                                                                                                                                                                    aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                                                                                                                                                                             >  5:338893/1‑149 (MQ=255)
                                                                                                                                                                                    aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATc                                                                                                                                                                                             >  7:132626/1‑149 (MQ=255)
                                                                                                                                                                                    aTTCACAAATCATGCCAGTTTTTAATTTACTGTTATATAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagtga                                                                                                                                                                                                          >  7:55310/1‑136 (MQ=255)
                                                                                                                                                                                       cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAg                                                            >  4:260865/1‑275 (MQ=255)
                                                                                                                                                                                           aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGac                                                                                                                                                                                                                                                                >  6:323908/1‑75 (MQ=255)
                                                                                                                                                                                           aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGac                                                                                                                                                                                                                                                                <  5:323908/75‑1 (MQ=255)
                                                                                                                                                                                           aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGttt                                                                                                                                                                                        <  8:250425/147‑1 (MQ=255)
                                                                                                                                                                                           aaTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGttt                                                                                                                                                                                        >  7:250425/1‑147 (MQ=255)
                                                                                                                                                                                            aTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                                                                                                                                        >  8:153586/1‑98 (MQ=255)
                                                                                                                                                                                            aTCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgatga                                                                                                                                                                                                                                        <  7:153586/98‑1 (MQ=255)
                                                                                                                                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgat                                                                                                                                                                                                                                          <  7:26665/90‑1 (MQ=255)
                                                                                                                                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtgat                                                                                                                                                                                                                                          >  8:26665/1‑90 (MQ=255)
                                                                                                                                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGa                                                                                                                                                                                                                     >  8:357091/1‑111 (MQ=255)
                                                                                                                                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGa                                                                                                                                                                                                                     <  7:357091/111‑1 (MQ=255)
                                                                                                                                                                                                  ccAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCACGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGaa                                                                                                                                                                               <  8:361748/149‑1 (MQ=255)
                                                                                                                                                                                                       ttttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGttt                                                                                                                                                                          <  6:169426/149‑1 (MQ=255)
                                                                                                                                                                                                         ttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCTCTGAAGGTTTTc                                                                                                                                                                        <  7:34879/149‑1 (MQ=255)
                                                                                                                                                                                                         ttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc                                                                                                                                                                        <  8:359769/149‑1 (MQ=255)
                                                                                                                                                                                                                       tttAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATc                                                                                                                                                          >  6:448421/1‑149 (MQ=255)
                                                                                                                                                                                                                       tttAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATc                                                                                                                                                          >  5:183115/1‑149 (MQ=255)
                                                                                                                                                                                                                          aaGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                                                                                                                                       <  8:132626/149‑1 (MQ=255)
                                                                                                                                                                                                                            ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTg                                                                                                                                                                                                                                                         <  6:252960/49‑1 (MQ=255)
                                                                                                                                                                                                                            ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTg                                                                                                                                                                                                                                                         >  5:252960/1‑49 (MQ=255)
                                                                                                                                                                                                                            ggAAATTAATTGCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGc                                                                                                                                                     >  7:308162/1‑149 (MQ=255)
                                                                                                                                                                                                                             gAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGcc                                                                                                                                                    >  6:69738/1‑149 (MQ=255)
                                                                                                                                                                                                                             gAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGcc                                                                                                                                                    >  8:2537/1‑149 (MQ=255)
                                                                                                                                                                                                                              aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGtga                                                                                                                                                                                                                                           >  4:517997/1‑61 (MQ=255)
                                                                                                                                                                                                                              aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                                                                                                                                        <  7:432794/80‑1 (MQ=255)
                                                                                                                                                                                                                              aaaTTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                                                                                                                                        >  8:432794/1‑80 (MQ=255)
                                                                                                                                                                                                                               aattaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                                                                                                                               >  6:475341/1‑136 (MQ=255)
                                                                                                                                                                                                                               aattaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTgag                                                                                                                                                               <  5:475341/136‑1 (MQ=255)
                                                                                                                                                                                                                                 ttaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                                                                                                                                                                                            <  6:330290/89‑1 (MQ=255)
                                                                                                                                                                                                                                 ttaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgagt                                                                                                                                                                                                            >  5:330290/1‑89 (MQ=255)
                                                                                                                                                                                                                                 ttaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCg                                                                                                                                                >  5:213856/1‑149 (MQ=255)
                                                                                                                                                                                                                                  taattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAc                                                                                                                                                                                                                                       <  2:518002/61‑1 (MQ=255)
                                                                                                                                                                                                                                  taattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGa                                                                                                                                               <  5:289032/149‑1 (MQ=255)
                                                                                                                                                                                                                                  ggaaagttgaaaTGCAGGAAAAAAGATGTCAGTGACAGTGGTGAAGATGACATGTGATGACAAGGTGTATCGGGAAGGAGCAATGAGTGAGTCGCTGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTTAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCGGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGATGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGc                  <  4:324828/264‑1 (MQ=255)
                                                                                                                                                                                                                                           gaaTGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaaaaa                                                                                                                                      <  7:2537/148‑1 (MQ=255)
                                                                                                                                                                                                                                              tGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCa                                                                                                                                   <  5:69738/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 aGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTAcgc  <  2:260869/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   gAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGacac                      >  2:324832/1‑253 (MQ=255)
                                                                                                                                                                                                                                                   gAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGATCTCGCACCGCTAcgc  >  2:216103/1‑273 (MQ=255)
                                                                                                                                                                                                                                                     aaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTcc                                                                                                                            >  7:28563/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      aaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCa                                                                                                                           >  7:17106/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       aaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaa                                                                                                                          >  6:150921/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        aCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                                                                                                                                       >  5:323565/1‑119 (MQ=255)
                                                                                                                                                                                                                                                        aCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATcccc                                                                                                                                                       <  6:323565/119‑1 (MQ=255)
                                                                                                                                                                                                                                                          gATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                                                                                                                                         >  5:301910/1‑83 (MQ=255)
                                                                                                                                                                                                                                                          gATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                                                                                                                                         <  6:301910/83‑1 (MQ=255)
                                                                                                                                                                                                                                                                aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt                                                                                                                 >  7:185706/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                               
TTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTGCTCTGGCACCGCTACGC  >  NC_000913/2837962‑2838477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: