| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,960,930 | G→C | 5.9% | S500C (TCC→TGC) | recC ← | exonuclease V (RecBCD complex), gamma chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,960,930 | 0 | G | C | 5.9% | 350.7 / 11.5 | 102 | S500C (TCC→TGC) | recC | exonuclease V (RecBCD complex), gamma chain |
| Reads supporting (aligned to +/- strand): ref base G (48/48); new base C (2/4); total (50/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-03 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGA > NC_000913/2960657‑2961203 | cAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa < 2:278055/275‑1 (MQ=255)cAAACCGGAAACCACCCTTCCAGCGGACGCTCCTGTGCCAGCTCGCGACGCCAGATGTTTAGCTGCAGTAGCAGTGAAGCCATATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACCCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGGGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa < 2:92511/275‑1 (MQ=255) cAACCAGCCCTCCGGCGGACGCTCCAGTGCCAGCCCGTGAGGCCAGATGTTTAGCTGCATTGGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGa < 2:305149/275‑1 (MQ=255) ttCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc < 2:133066/275‑1 (MQ=255) gCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTTCCACTCGCCCTGCGCGCTCCCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCCCTCGAACGTTGTCGTCATCTATGCACCAACGAATGCTGGATTCGTTGACCCCCTGGCGTaa > 2:11061/1‑274 (MQ=255) gACGCTCCTGTGCCAGCCAGCGACGCAAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTATCCAATTAAGACGCTCGATTCATCATAAGGTAGAACCGATTTCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCCACAACATACGCGACAGGCCAAAACGCCAGGGGTTGTGTCCGATGGCGGGGAGTTCCAGCTCGCGAACGTTAACGTCCTTTATGCCCCACCGAAAGCCAGATGAGTTCACCCGCTGGCCTAGATat > 4:460490/1‑274 (MQ=255) cTGTGCCAGCCCGCGACTCCAGACGTTTAGTTTCAATAGCAGTTAAGCCAGATGCCCCACCAGTTCTCCAATTAAGCCGGCGGATTCATCATAAGGTAGAACCGATTGCCACGCGACCTGCGCGCTCTCCATCGCGTAGCCCGACACCATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGcc < 4:277281/275‑1 (MQ=255) gCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGc > 2:277285/1‑263 (MQ=255) cATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCCAACCGCGCCGCCAGCa > 2:170583/1‑275 (MQ=255) cATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGACGGATTCGTTGACCCACTGGCGTACATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCTCCGCCAGCa > 2:144372/1‑275 (MQ=255) gTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGcaca < 2:314384/275‑1 (MQ=255) aaGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTTACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGCCAAACCGCGCCGCCAGCACCGGCACATcc > 2:462334/1‑275 (MQ=255) ccAGATGCCCCACCAGTGCGGCAATTAAGCCGCTCGATTCATCATAAGGGAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAAAAACATACGCGTCAGGCCAAAGCGCAAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGATCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc < 1:87594/213‑1 (MQ=255) tGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGCTGTCAAACCGCGCCGCCAGCCCCGGCACCTCCAGCACCGCCAGCCCATcc > 1:52400/1‑271 (MQ=255) gATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa < 1:103685/173‑1 (MQ=255) gTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGAt < 5:119066/149‑1 (MQ=255) tAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa < 5:290327/147‑1 (MQ=255) tAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa > 6:290327/1‑147 (MQ=255) gagccGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCGAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCg > 8:318799/4‑149 (MQ=255) gAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa < 4:529101/145‑1 (MQ=255) gAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCg > 8:16424/1‑149 (MQ=255) aaCCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGt < 5:417511/149‑1 (MQ=255) aaCCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGt < 5:369192/149‑1 (MQ=255) aCCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGtt < 5:116804/149‑1 (MQ=255) ccGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGGCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATCCGGCATTCGTTg < 6:268760/149‑1 (MQ=255) gATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCa < 2:228174/205‑1 (MQ=255) agtGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACGTACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATa < 2:486450/273‑1 (MQ=255) aTTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACCCAAAACGACTGTCAGGAAGTCATAACACGCTGAt > 2:441095/1‑274 (MQ=255) aTTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGCAAATAACGCAGCCCTTCTTCGGTGATGTTAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATTCTCTGACCAAAAACGACTGTCAGGCAGTGATAACAGGCTGATa > 2:58904/1‑275 (MQ=255) cACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTg < 6:293005/149‑1 (MQ=255) cACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATCCGGCATTCGTTggggcata < 5:327628/148‑8 (MQ=255) cACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATCCGGCATTCGTTggggcata > 6:327628/1‑141 (MQ=255) aCTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTgg > 7:33846/1‑149 (MQ=255) aCTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑c < 2:169151/272‑1 (MQ=255) cGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGt < 6:185220/149‑1 (MQ=255) tGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGAt > 7:414037/1‑126 (MQ=255) tGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGAt < 8:414037/126‑1 (MQ=255) gcTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCa > 6:324376/1‑149 (MQ=255) ctctCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGACGTCATCTATGCCCCAACGAATGCCGGACTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTATGTGATGTCAAACCGCCCCGCCAGCACCGGCACATCCCGCAACGCCAGCACCTCACCTCACACAAACCCACCGACAGGCATTGATAACAGCGAGATAAA‑CGCTTcagggaccagag > 4:226938/1‑263 (MQ=255) tctcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGc > 5:183804/1‑149 (MQ=255) ctcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATTCCGGATTCGTGGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGAGGTCAAACCGCGCCGCCAGCACAGGCACATCCAGCACCGCCAGCACATCCTCAGCCCCAACACTACTGTCAGACAGTAATAACAGGC‑GACAAACCGCTTCCAGTACCGGAtgag > 4:119610/1‑273 (MQ=255) tcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGccc > 6:195780/1‑149 (MQ=255) cATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAAc < 8:195424/140‑1 (MQ=255) cATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAAc > 7:195424/1‑140 (MQ=255) cATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCctt > 8:312860/1‑149 (MQ=255) gCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa < 7:318799/149‑1 (MQ=255) ctcaacaaCATACGCGTCAGGCCAAATCGCGAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGAt < 7:388275/147‑1 (MQ=255) aacaacATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTc > 5:21757/1‑149 (MQ=255) acaacaTACGCGTCAGGCCAAATCGCCAGGTGTGTTGGCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCGTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGc < 2:456309/179‑1 (MQ=255) cagcagaCGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGTGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCGTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGt < 2:140387/269‑1 (MQ=255) tACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATCAA‑CGCTTCAAGTACCGGAGGTGACTGCCGCGCACGACGGTCGGaa > 2:56203/1‑275 (MQ=255) tACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACAACCTCTGACACAAAACGACTGTCAGGCAGCGAATACAGGCTCATAAA‑CGCTTCCCGTACCGGATCTGAATGCGCCGCACGACGTGCGGaa > 4:433823/1‑275 (MQ=255) tCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCg > 2:34204/1‑275 (MQ=255) aTCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAaggtagg < 2:119611/275‑1 (MQ=255) aTCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACAACCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGACGGAAATGGCGTAaggtagg > 2:259450/1‑275 (MQ=255) cAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATc < 5:324376/149‑1 (MQ=255) ggTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCa > 5:23598/1‑149 (MQ=255) tgttgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACaaa > 4:451363/1‑173 (MQ=255) tggtgcccggggccggggAGTTGCGGCTNGNGACCGTGTTCGTCAGTTAGGCCCCAACGAAGGCCGGATGCTGTGCNCCACGAGCGTAACTAACGCAGCCCTGATTCGGGGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGNAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGAAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACGGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGc < 3:52400/269‑1 (MQ=255) gttgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAAc > 7:97820/1‑149 (MQ=255) gttgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAAc < 7:62547/149‑1 (MQ=255) gtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGcc > 5:261989/1‑149 (MQ=255) gtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGcc > 7:443574/1‑149 (MQ=255) gtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGa < 2:451368/173‑1 (MQ=255) gtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑c < 2:7053/203‑1 (MQ=255) tCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTg < 2:433828/275‑1 (MQ=255) cGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGc > 6:123409/1‑149 (MQ=255) ggTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCAACGGCACATCCAGCAACGCCAg > 4:330331/1‑147 (MQ=255) gTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa > 8:356003/1‑103 (MQ=255) gTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa < 7:356003/103‑1 (MQ=255) tGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCa > 4:494945/1‑123 (MQ=255) gCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGcaca < 2:330335/147‑1 (MQ=255) gCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATc > 7:196808/1‑149 (MQ=255) ggggAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCgg < 2:494950/123‑1 (MQ=255) gAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTg < 6:291291/149‑1 (MQ=255) cgcgAACGTTGTCGTCATATATTCCCCAACGAATGCCGGATTCGTGGACCCACTGGCGTAAATAACGCAGACCTTCGTCGGTGATGTCAAACCGCGCCGCCAGCACCGACACACCCAGCAACGCCAACACATCCTCTGCCACACAACGCCTGTCAGGCAGTGATAACAGGCGGATAAA‑CGCTTCCAGTACCGGATGTGAACGCAGCGCCCTACGGTCGGACACCGCATAAGGTAGGTAACGAGCCTCAGTCTCGCAACCACAGACAGCCTGAAt > 4:164885/1‑274 (MQ=255) cgAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACt < 7:421115/149‑1 (MQ=255) cgAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACt < 7:312860/149‑1 (MQ=255) gAACGTTGTCGTCAACTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCAGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATaaa < 2:62152/274‑1 (MQ=255) cgtcATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAg < 2:226942/275‑1 (MQ=255) aTCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATa > 5:87702/1‑149 (MQ=255) tCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATaa < 6:5635/149‑1 (MQ=255) tATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCctct < 7:123228/118‑1 (MQ=255) tATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCctct > 8:123228/1‑118 (MQ=255) tATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACa > 6:369318/1‑149 (MQ=255) tATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACa > 6:192862/1‑149 (MQ=255) tGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGccacca > 2:481072/1‑239 (MQ=255) ccccAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCt < 8:355535/149‑1 (MQ=255) tcAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAgg < 5:461708/144‑1 (MQ=255) tcAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAgg > 6:461708/2‑145 (MQ=255) tcAACGAATCCGGCATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGa > 7:27650/2‑149 (MQ=255) ccAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGAt > 5:12985/1‑139 (MQ=255) ccAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGAt < 6:12985/139‑1 (MQ=255) cAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGAt > 2:494035/1‑138 (MQ=255) cAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGAt > 7:427897/1‑149 (MQ=255) aaCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATa > 6:143937/1‑149 (MQ=255) aaCCAATGCCGGAGTCGGTGACCCACGGGCGTAACTAACGCAGACCTTCCTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCCGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGcc < 4:34204/275‑1 (MQ=255) gAAGGCCGGATTCGTTGACCCACTGACGTAAATACCGCAGCCCTTCTTCGGGGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAAGGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGGGATAACa < 4:494030/138‑1 (MQ=255) ggATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAaggtagg < 2:183236/199‑1 (MQ=255) ggATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCGGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACga < 2:19884/275‑1 (MQ=255) | CAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAA‑CGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGA > NC_000913/2960657‑2961203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |