Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,353 A→G 5.8% R149R (CGT→CGC hybE ← hydrogenase 2‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,3530AG 5.8% 318.1 / 6.4 103R149R (CGT→CGChybEhydrogenase 2‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base A (44/50);  new base G (2/5);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

Only 100 of 103 total aligned reads displayed.

ACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAG  >  NC_000913/3140083‑3140616
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                       
accTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAACACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGCCGCTCTTGCAACGAGACCCCTCATGCATTTTCGCCACTCCTGCGACCCAACCGCAGGGCGCTACGcc                                                                                                                                                                                                                                                                     <  2:237066/275‑1 (MQ=255)
 ccTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATACTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTCCGAACAAAAAGCAGGGCCCGACGcc                                                                                                                                                                                                                                                                     >  4:75800/1‑274 (MQ=255)
      cTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTAGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTATCCTCAACGCAGGAAAGCGCGACAAATTCCAGCCACACGGCGGTGACGCGCTGAAAATAGTGATGCTCCGCCTGCCGTTGTGAAACTTCAACGGAGCTCTGGCCACGGTGCAAAGCATGCATTGTGGCCACTCCCGCTACACCACAGTAGGGCTCCAAGCCCTCCgt                                                                                                                                                                                                                                                               >  4:113128/1‑275 (MQ=255)
      cTGCAATCCCACCACCGCGCGTGCGAGGTNTTAGAGAAGATACGTAAATCGCCCANGTGCGCCACCGTTCCTTGGCAGACAATTTCAAACCTGAAACGGCAGGCGCTCCCCTCAACGCAGGAGAGCGCGCCAAGTTNCAGCCACACTGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgt                                                                                                                                                                                                                                                               <  3:92145/275‑1 (MQ=255)
                 gCACCAAGCCTGGCCGGGTTAATTAACGATATGTAAATCGGACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATcc                                                                                                                                                                                                                                                    <  2:185610/275‑1 (MQ=255)
                       aGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTGCGTTGAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACCTCGTGCTTCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCCCTCCTGCGTCCCAACCACAGGGCGCGACGCCCGGCGTGTGGtcatttccgcttc                                                                                                                                                                                                                                              >  2:58378/1‑263 (MQ=255)
                                            gATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATc                                                                                                                                                                                                                         >  2:111111/1‑275 (MQ=255)
                                                 gTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCCCGACGCCCTg                                                                                                                                                                                                                                                                  >  4:443864/1‑229 (MQ=255)
                                                     accGCACCCTTTCGCAACCGTTCCGTGGCAACCAATTTCAAAACGAAAACGGACGGCGCTCTCCTCAACGCAGAAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAATAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                                                                                                                                                <  2:113129/273‑1 (MQ=255)
                                                     aaacagcccTTGCGCCACCTGTCCTTGGCAAACAATTTCAAAATAAAACGGGACGGGCTCTTCCTCAACGCAGGAGGGCCCGCCAATTTGCAGCCACACGGCGGTGACGCGCTTAACTTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTGGCCACTCCTGCGACCAAACGGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                                                                                                                                                <  4:346847/269‑1 (MQ=255)
                                                     aTCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgtg                                                                                                                                                                                                                                                              <  2:443869/229‑1 (MQ=255)
                                                         cACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTTACTGGCACCGAAAGGATCATTCGTGCGCAGt                                                                                                                                                                                                            >  2:242314/1‑275 (MQ=255)
                                                          aCCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACCCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCTGCGCAAGGATCATTCGTTCGCAGTc                                                                                                                                                                                                           >  2:38903/1‑275 (MQ=255)
                                                           cccTCGCCCCCCCGCTCCGTGCCCACCAANTCCCAACCCAAACCGGCCGGCCCACNCCTCACCGCAGGAGGGCGGGCCCATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGNGAATTTCAACGGCGCTCTGGCAAAGAGACGACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCa                                                                                                                                                                                                          <  3:101209/275‑1 (MQ=255)
                                                               tGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTTCGCa                                                                                                                                                                                                              >  1:101209/1‑267 (MQ=255)
                                                                      ccGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                                                                                                                                                    >  2:346852/1‑254 (MQ=255)
                                                                              tGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGACCATTCGTGCGCAGTCATCCGTCAGGCGTTGGccc                                                                                                                                                                                        >  2:91463/1‑274 (MQ=255)
                                                                                  aGAAAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                                                                                                                                                   <  2:116004/275‑1 (MQ=255)
                                                                                                   aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGCGACAACTCATGCCTTTTCGCCACCCCTGCGACCAAACAGCAGGGCCCGCCGCCCTGCCTGTGGTCCATCCGGATTCGTGAATGGGCGCGAAAGGATCCTTCGTTGCCCGTCACCCGTCAGTCCTTGGCCCCCTGTAAGCGACATGCtttag                                                                                                                                                                   >  4:44385/1‑270 (MQ=255)
                                                                                                                            cgaggcgggcgcccCAATTCCCAGCCCACCGGCGGTGCCCCCCTTAACATCTTGCTTCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAg                                                                                                                                           <  2:55654/267‑1 (MQ=255)
                                                                                                                                     cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGct                                                                                                                                                                                                                                                                <  8:236576/149‑2 (MQ=255)
                                                                                                                                     cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATACATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGct                                                                                                                                                                                                                                                                <  7:325117/149‑2 (MQ=255)
                                                                                                                                        gcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCgtgt                                                                                                                                                                                                                                                             >  6:333201/1‑149 (MQ=255)
                                                                                                                                         ccAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCg                                                                                                                                                <  2:487770/257‑1 (MQ=255)
                                                                                                                                          cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTgg                                                                                                                                                                                                                                                           <  8:235437/149‑1 (MQ=255)
                                                                                                                                           aaTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                                                                                                                                                                                                                          >  5:42784/1‑149 (MQ=255)
                                                                                                                                             tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAATGCGGCTACATCAg                                                                                                                                                  >  2:500605/1‑251 (MQ=255)
                                                                                                                                               tCCAGCCACACGGCGGGGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAaca                                                                                                                         <  1:143847/274‑1 (MQ=255)
                                                                                                                                                ccagcccatgggcggTGACGCGCTGACCATCCTGCTGGTCGGCCTGCCGTTGGAGAATTTCAACGGCGCTCTGGCAAAGAGACACCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACAcc                                                                                                                       <  4:74501/265‑1 (MQ=255)
                                                                                                                                                       cacGGCGGGGACGCGCTTAACATCGTGCTGCGCGGCCAGCCGTTGGATAATTTCAACGGCGCTCTGGAAAAGAGACAACTCATGCATTTTCGCCAATCCTGCGACCAAACGGCAGGGCGCGACGCCCTGCGTGGGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATAGCGAAACACCGTCCAGt                                                                                                                <  2:34707/275‑1 (MQ=255)
                                                                                                                                                        acGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGaaa                                                                                                                           >  2:74502/1‑263 (MQ=255)
                                                                                                                                                            cggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGcc                                                                                                           <  2:75801/275‑1 (MQ=255)
                                                                                                                                                               tGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTgg                                                                                                                                                                                                                                      <  7:122014/149‑1 (MQ=255)
                                                                                                                                                                 aCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCTAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGGACATCCGGATTCGTGACTGGCa                                                                                                                                                                                                                                    >  5:408368/1‑149 (MQ=255)
                                                                                                                                                                   gcgcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGc                                                                                                                                                                                                                                  >  8:266788/1‑149 (MQ=255)
                                                                                                                                                                     gcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                                                                                                                                                                                                                >  7:403072/1‑149 (MQ=255)
                                                                                                                                                                     gcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                                                                                                                                                                                                                >  6:399923/1‑149 (MQ=255)
                                                                                                                                                                      cTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGaa                                                                                                                                                                                                                               >  6:287478/1‑149 (MQ=255)
                                                                                                                                                                        tAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAg                                                                                                                                                                                                                             <  5:333201/149‑1 (MQ=255)
                                                                                                                                                                           catcatGCTGTGCCGTCTGGCGTAGTAAAATATCAACGGCGCTCTGGCAAGGGGATGACGCATGCATTTACGCCACCCCTGGCACCAACCAGCAGGGCGCGGCGCCTTGCGTGTGGTGACTCCGGGTTCGTGACTGGCAGCGAACGGATCATTCTGGCGCAATCATCCGTCAGGCGTTGGCCCTCTTCAATCAACATGCTGGGCGAGAGCGGCGACATCAGCGAACAGGAGGGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCa                                                                                             <  4:440461/274‑1 (MQ=255)
                                                                                                                                                                           cATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGCAt                                                                                                                                                                                                                          >  7:418920/1‑149 (MQ=255)
                                                                                                                                                                           cATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCAACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                                                                                                                                          <  6:196130/149‑1 (MQ=255)
                                                                                                                                                                           actcgtcgtgctCCGCCTGCCCTGGGCTAAATTCACCGGCGCGCTGGAAAAGAGACACGCACTGCATTTTCGCCACTCCTCCGACCAAACAGCAGGGGGAGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCa                                                                                             <  4:111110/266‑1 (MQ=255)
                                                                                                                                                                              cGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCGGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAg                                                                                          <  2:36479/274‑1 (MQ=255)
                                                                                                                                                                                 gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                                                                                                                                                    >  5:460873/1‑149 (MQ=255)
                                                                                                                                                                                   tgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCg                                                                                                                              <  2:98067/233‑1 (MQ=255)
                                                                                                                                                                                   tgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCa                                                                                     <  2:208765/274‑1 (MQ=255)
                                                                                                                                                                                     ctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTTCCATa                                                                                   >  2:129744/1‑274 (MQ=255)
                                                                                                                                                                                      tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGct                                                                                                                                                                                                                                                                <  6:352635/100‑2 (MQ=255)
                                                                                                                                                                                      tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGct                                                                                                                                                                                                                                                                >  5:352635/1‑99 (MQ=255)
                                                                                                                                                                                       ccGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                                                                                                                                              <  6:300327/149‑1 (MQ=255)
                                                                                                                                                                                       ccGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                                                                                                                                              >  5:291516/1‑149 (MQ=255)
                                                                                                                                                                                            tGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCAt                                                                                                                                                                                                         <  6:42784/149‑1 (MQ=255)
                                                                                                                                                                                                    gATAATTTCAACGGCGCTCTTGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCTAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGAcc                                                                    <  1:51746/274‑1 (MQ=255)
                                                                                                                                                                                                     aTAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTgg                                                                                                                                                                                                                                                           >  8:387009/1‑90 (MQ=255)
                                                                                                                                                                                                     aTAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTgg                                                                                                                                                                                                                                                           <  7:387009/90‑1 (MQ=255)
                                                                                                                                                                                                      tAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCg                                                                                                                                                                                               >  7:205977/1‑149 (MQ=255)
                                                                                                                                                                                                             aaCGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGccc                                                                                                                                                                                        >  6:379011/1‑149 (MQ=255)
                                                                                                                                                                                                               cGGAGCTCGGGCAAAGTGAAAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTacac                                                         <  2:213882/274‑1 (MQ=255)
                                                                                                                                                                                                                ggCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCctct                                                                                                                                                                                     <  7:250194/149‑1 (MQ=255)
                                                                                                                                                                                                                  cgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTNGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCCACATCAGCGAACAGGAGAGATATTGCGAAACAACTTCCACTTCCNCACCGGTAAAGGTCACAGTGCCAAANACCAGTTGAAGACACATTTTTtcactccc                                                     >  3:89514/1‑273 (MQ=255)
                                                                                                                                                                                                                    ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACtt                                                   >  2:396032/1‑275 (MQ=255)
                                                                                                                                                                                                                                gaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgt                                                                                                                                                                     <  5:287478/149‑1 (MQ=255)
                                                                                                                                                                                                                                  cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGc                                                                                                                                                                   <  6:460873/149‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                                                                                                                                                  <  5:88286/149‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGGAACACCGTCCAGTTCGCCAACGGCAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCaga                                    >  2:71135/1‑275 (MQ=255)
                                                                                                                                                                                                                                   aaCTCATGCATTTTCGCCACTACTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGCAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTCCGAAAGCGGCGACATCAGCCAACCGGACAGATATTGCGAACCACCGTCCAGTCCGCCAACGGTAAAGGTCATAGTGCCATACGGAAGTTGCAGACCAATTTTTTCACACCCATTGCCCAGAGCCCacg                                    >  4:179044/1‑273 (MQ=255)
                                                                                                                                                                                                                                     cTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCAt                                                                                                                                                                                                                       <  8:321910/94‑1 (MQ=255)
                                                                                                                                                                                                                                     cTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCAt                                                                                                                                                                                                                       >  7:321910/1‑94 (MQ=255)
                                                                                                                                                                                                                                      tCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGaaa                                                                                                                                                               >  7:445465/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGaaa                                                                                                                                                               >  5:469920/1‑149 (MQ=255)
                                                                                                                                                                                                                                        aTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgc                                                                                                                                                             <  8:418920/149‑1 (MQ=255)
                                                                                                                                                                                                                                             aTTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                                                                                                                                         >  7:348993/1‑149 (MQ=255)
                                                                                                                                                                                                                                              ttttCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGa                                                                                                                                                        >  7:94385/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  cGCCACCCCGGCGACCAAAAAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATccgggccg                      <  4:396027/275‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTTCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATccgggccggg                    <  1:89514/275‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATc                                                                                                                                                    <  5:179471/147‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACg                                                                                                       >  2:510310/1‑192 (MQ=255)
                                                                                                                                                                                                                                                     cACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGc                                                                                                                                                 <  6:408368/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCg                                                                                                                                                <  5:314165/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCg                                                                                                                                                <  7:19932/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        tCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGtt                                                                                                                                                                                             >  1:81848/1‑102 (MQ=255)
                                                                                                                                                                                                                                                        tCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                                                                                                                                              >  6:273610/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        tCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTaa                                                                                                   <  4:510305/192‑1 (MQ=255)
                                                                                                                                                                                                                                                            gCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGcc                                                                                                                                                                                         <  3:81848/102‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAg                                                                                                                                                  >  2:168465/1‑140 (MQ=255)
                                                                                                                                                                                                                                                             cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGga                                                                                                                                         <  6:13632/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                                                                                                                                              <  4:168463/140‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cacacgcagGGCGTCGCGCCCTGCTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                                                                                                                                          <  6:432204/121‑1 (MQ=255)
                                                                                                                                                                                                                                                                   caCAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGGGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACt     <  2:484376/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGAc                                                                                                                                                       >  1:152179/1‑129 (MQ=255)
                                                                                                                                                                                                                                                                    cacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGga                                                                                                                                         <  5:48206/139‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGga                                                                                                                                         >  6:48206/4‑142 (MQ=255)
                                                                                                                                                                                                                                                                      agcagGGTGCGACGTCGCGCAGGGGGGAGATACGGGTTAGAGACTGGCAGCGAAAAGATCACTGGTGGGGAGTCATCCGTCAGGAGTTGGCCCTAGTAAATCGACATGCTGTGGGAAAGCTGCGACATAAGCGAACAGGAGGGATACTGCGAAACACCGTACAGGTCGACAACGGTAACGGTCATAGTGCCATACGGCAGTTGCAGACTAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGGTAACTGCACTCAg  <  4:242310/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                      agcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAg  <  2:44385/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                      agcagGGCGCGACGCCCTGCGTGTGGTACAGCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAAGCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAg  <  2:61661/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCa                                                                                                                                                   <  3:152179/129‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGaaa                                                                                                                           <  6:469920/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGaaa                                                                                                                           >  6:46387/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                                cgACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGt                                                                                                                     >  4:320319/1‑151 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                       
ACCTGGCTGCAATCCCAGCACCAAGCCTGGGCGGGTTTATAGACGATATGTAAATCGCACCCTTGCGCCACCGTTCCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGTCCAGTTCGCCAACGGTAAAGGTCATAGTGCCATACGGCAGTTGCAGACCAATTTTTTCACTCACTTTGCGCAGCGGCCAGAGTTGATCCGGGCCGGGGAAGATAACTGCACTCAG  >  NC_000913/3140083‑3140616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: