Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,192,129 T→A 5.9% intergenic (‑190/‑79) glgS ← / → yqiJ motility and biofilm regulator/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,192,1290TA 5.9% 231.7 / 3.8 67intergenic (‑190/‑79)glgS/yqiJmotility and biofilm regulator/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base A (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

ACCTTCTGCGTGCATTCTGGCAAAACTACGCGCCAGGAAATCGAAATTATTTAAAGAATTAAGACTATGATCCATAAGCACATCCTCCGGTTATCGCATACCTTTTAAGGCCATATGCTTTTTCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAATTTTTGCGCTTATCTGCGGTCACATGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGC  >  NC_000913/3191865‑3192372
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
aCCTTCTGCGTGCATTCTGGCAAAACTACGCGCCAGGAAATCGAAATTATTTAAAGAATTAAGACTATGATCCATAAGCACATCCTCCGGTTATCGCATACCTTTTAAGGCCATATGCTTTTTCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAac                                                                                                                                                                                                                                              <  2:402111/272‑1 (MQ=255)
                                                                                                                             gATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAacac                                                                                                                                                                                                                                            <  8:456517/149‑1 (MQ=255)
                                                                                                                               tttCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTAATAATAACACAg                                                                                                                                                                                                                                          <  7:246042/149‑1 (MQ=255)
                                                                                                                                     tATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAAt                                                                                                                                                                                                                                    <  5:28438/149‑1 (MQ=255)
                                                                                                                                     tATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTAATAATAAcaca                                                                                                                                                                                                                                           >  6:297183/1‑142 (MQ=255)
                                                                                                                                     tATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTAATAATAAcaca                                                                                                                                                                                                                                           <  5:297183/142‑1 (MQ=255)
                                                                                                                                          cccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                                                                                                                                                                                               >  7:227568/1‑133 (MQ=255)
                                                                                                                                          cccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                                                                                                                                                                                               <  8:227568/133‑1 (MQ=255)
                                                                                                                                          cccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                                                                                                                                                                                                               >  5:50751/1‑149 (MQ=255)
                                                                                                                                          cccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                                                                                                                                                                                                               >  8:126942/1‑149 (MQ=255)
                                                                                                                                                        tGCCTTTAAACATAACGTTCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa                                                                                                                                                                                                                 <  7:205789/149‑1 (MQ=255)
                                                                                                                                                        tGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatt                                                                                                                                                                                                                                                    >  6:202873/1‑114 (MQ=255)
                                                                                                                                                        tGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatt                                                                                                                                                                                                                                                    <  5:202873/114‑1 (MQ=255)
                                                                                                                                                            tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                                                                                                             >  7:325209/1‑149 (MQ=255)
                                                                                                                                                            tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                                                                                                             <  5:38005/149‑1 (MQ=255)
                                                                                                                                                            tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                                                                                                             <  5:293707/149‑1 (MQ=255)
                                                                                                                                                            tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                                                                                                             >  5:276934/1‑149 (MQ=255)
                                                                                                                                                               aaaCATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                                                                                                                                                                                                               >  7:31570/1‑128 (MQ=255)
                                                                                                                                                               aaaCATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                                                                                                                                                                                                               <  8:31570/128‑1 (MQ=255)
                                                                                                                                                               aaaCATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTa                                                                                                                                                                                                          >  8:79970/1‑149 (MQ=255)
                                                                                                                                                                   aTAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAata                                                                                                                                                                                                      >  7:45875/1‑149 (MQ=255)
                                                                                                                                                                         tGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt                                                                                                                                                                                                <  8:357565/149‑1 (MQ=255)
                                                                                                                                                                                   tatTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATgg                                                                                                                                                                                      <  8:51064/149‑1 (MQ=255)
                                                                                                                                                                                          cccGATCACACTTTGTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTgg                                                                                                                                                                               >  7:35643/1‑149 (MQ=255)
                                                                                                                                                                                           ccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAAc                                                                                                                                                                                                                      <  5:428872/109‑1 (MQ=255)
                                                                                                                                                                                           ccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAAc                                                                                                                                                                                                                      >  6:428872/1‑109 (MQ=255)
                                                                                                                                                                                           ccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGa                                                                                                                                                                              <  6:91767/149‑1 (MQ=255)
                                                                                                                                                                                             gATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATaaa                                                                                                                                                                                                                                     <  7:436180/92‑1 (MQ=255)
                                                                                                                                                                                             gATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATaaa                                                                                                                                                                                                                                     >  8:436180/1‑92 (MQ=255)
                                                                                                                                                                                                cacaCTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGat                                                                                                                                                                         <  6:417665/149‑1 (MQ=255)
                                                                                                                                                                                                cacaCTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGat                                                                                                                                                                         <  8:45875/149‑1 (MQ=255)
                                                                                                                                                                                                  cacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATgg                                                                                                                                                                                      >  4:515251/1‑134 (MQ=255)
                                                                                                                                                                                                      ttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt                                                                                                                                                                                  <  2:515256/134‑1 (MQ=255)
                                                                                                                                                                                                                aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTTGc                                                                                                                                                         >  7:390889/1‑149 (MQ=255)
                                                                                                                                                                                                                aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGc                                                                                                                                                         <  8:325209/149‑1 (MQ=255)
                                                                                                                                                                                                                 aaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGcc                                                                                                                                                        <  7:126942/149‑1 (MQ=255)
                                                                                                                                                                                                                      aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc                                                                                                                                                                                                  <  8:188073/102‑1 (MQ=255)
                                                                                                                                                                                                                      aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                                                                                                                   >  6:144088/1‑133 (MQ=255)
                                                                                                                                                                                                                      aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                                                                                                                   <  5:144088/133‑1 (MQ=255)
                                                                                                                                                                                                                      aaaaCCATATTAAAAGACTTTCTCTTACAGAAATTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAATGTAAATATTGc                                                                                                                                                                                                  >  7:188073/1‑102 (MQ=255)
                                                                                                                                                                                                                        aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAg                                                                                                                                                                             >  6:245302/1‑121 (MQ=255)
                                                                                                                                                                                                                        aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAg                                                                                                                                                                             <  5:245302/121‑1 (MQ=255)
                                                                                                                                                                                                                         aCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATaa                                                                                                                                                <  6:50751/149‑1 (MQ=255)
                                                                                                                                                                                                                            atatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAacac                                                                                                                                             >  7:417355/1‑149 (MQ=255)
                                                                                                                                                                                                                                  aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                                                                                                                       >  6:194671/1‑149 (MQ=255)
                                                                                                                                                                                                                                  aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                                                                                                                       >  5:97519/1‑149 (MQ=255)
                                                                                                                                                                                                                                        tttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCtttt                                                                                                                                 >  5:24633/1‑149 (MQ=255)
                                                                                                                                                                                                                                         ttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAAATTTTGCGCTTATCTGCGGTCACATGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGCTTCTATCACCCAAGGTCATATTAGc  >  1:54131/1‑275 (MQ=255)
                                                                                                                                                                                                                                          tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAAt                                                                                                                                                                                        >  6:186911/1‑92 (MQ=255)
                                                                                                                                                                                                                                          tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAAt                                                                                                                                                                                        <  5:186911/92‑1 (MQ=255)
                                                                                                                                                                                                                                            tctTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCt                                                                                                                             >  5:342511/1‑149 (MQ=255)
                                                                                                                                                                                                                                            tctTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCt                                                                                                                             >  6:171436/1‑149 (MQ=255)
                                                                                                                                                                                                                                              ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTAt                                                                                                                           >  7:55863/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTcc                                                                                                                       >  5:245089/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    aaCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCtt                                                                                                                     <  6:276934/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     aCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCttt                                                                                                                    <  6:24633/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     aCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCttt                                                                                                                    <  8:35643/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATg                                                                                                                                                                                       <  7:213484/80‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATg                                                                                                                                                                                       >  8:213484/1‑80 (MQ=255)
                                                                                                                                                                                                                                                        tctcTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTa                                                                                                                 <  6:342511/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGAtt                                                                                                                                                                 >  7:469375/1‑99 (MQ=255)
                                                                                                                                                                                                                                                          tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGAtt                                                                                                                                                                 <  8:469375/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tgtgTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATc                                                                                                        <  8:417355/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tgtgTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATc                                                                                                        >  6:27554/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  gtgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCg                                                                                                       <  7:79970/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tgtTATTATTAACACAGATCAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAATTTTTGCGCTTATCTGCGGTCACATGCTCTCCGGTGCTCTTGATTCACAt                                         >  2:501562/1‑210 (MQ=255)
                                                                                                                                                                                                                                                                   tgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAtt                                                                                                                                                            >  3:20130/1‑95 (MQ=255)
                                                                                                                                                                                                                                                                   tgtTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                                                                                                                       >  2:268831/1‑116 (MQ=255)
                                                                                                                                                                                                                                                                    gtTAATAATAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATNACACCCCTTATCTTTTCGCTATTNNCTTTGTACNTTTGATCGGCCTGTAAGAAATTTTTGCGCTTNTc                                                                             >  3:35436/1‑173 (MQ=255)
                                                                                                                                                                                                                                                                      tattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTGTTTCCTTTGTACTGTTGATCGGCCt                                                                                                   <  8:390889/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTCCTGTTGATCGGCCt                                                                                                   <  6:97519/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       attattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGcc                                                                                                                                                        <  1:20130/95‑1 (MQ=255)
                                                                                                                                                                                                                                                                       attattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCtt                                                                                                                                   <  4:268827/116‑1 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
ACCTTCTGCGTGCATTCTGGCAAAACTACGCGCCAGGAAATCGAAATTATTTAAAGAATTAAGACTATGATCCATAAGCACATCCTCCGGTTATCGCATACCTTTTAAGGCCATATGCTTTTTCTGATTTCAGTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAATTTTTGCGCTTATCTGCGGTCACATGCTCTCCGGTGCTCTTGATGCACATCTTGACCATTATGATTCTATAACCACAGGTCATATTAGC  >  NC_000913/3191865‑3192372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑