| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,339,833 | G→A | 5.4% | S78L (TCG→TTG) | mlaF ← | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,339,833 | 0 | G | A | 5.4% | 361.2 / 6.7 | 111 | S78L (TCG→TTG) | mlaF | ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (59/45); new base A (2/4); total (61/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
CCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCT > NC_000913/3339576‑3340107 | ccGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGCAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGa > 1:114446/1‑275 (MQ=255) cgcAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCCCa > 2:166780/1‑274 (MQ=255) tgggatggcgcTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa > 2:300891/8‑274 (MQ=255) cAGCGCTGCCAGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtct < 2:460119/275‑1 (MQ=255) gcTGCACGCCGCGCCATCCAACCGGAAAGTTCCAAAGGCATCAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTATGATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc < 4:300887/275‑1 (MQ=255) cAGAAGGCATTATTTTAGCCGCACCCAGNGNGCCCACGGCCTCAAGCTTCATCATCCCCGTACTATGCAACAAGGGNGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACNCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGa < 3:42799/275‑1 (MQ=255) cgacgcACGGAGCCCCACGCACCCTAGCTNNAACATCAACCGACGATGCAACAATGGCGCGNAGAGTTGGGTATGTTAGCGCAGTGGATAGGCGACGTTGTAAAATACGTTCATATCATTGAACAACGACCCGGACNGAAATAACCTGCTCATCCGGTTGCGCACTGTAGACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAc < 3:60647/270‑1 (MQ=255) cTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGCTCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAcc > 2:12481/1‑274 (MQ=255) tCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATCCCG‑GCGCGAACGAGACATCCCCGCAATATTCCCACCATCGAACAGGATCCCACCATGAACTGGTGCGCTTTGCCCGCCAATCAAGCGGACTCGCGTCGTTTTACCGa > 4:129949/1‑275 (MQ=255) gCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc < 2:346721/223‑1 (MQ=255) tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTGATCCGTTTGCGCACTGTATACAG‑GCGCGaa > 5:309038/1‑149 (MQ=255) catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAAc < 8:125995/149‑1 (MQ=255) atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg > 8:212454/1‑149 (MQ=255) atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg > 8:332774/1‑149 (MQ=255) tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACgg < 5:34897/149‑2 (MQ=255) aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACgaga < 6:289526/149‑1 (MQ=255) ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt < 2:129950/275‑1 (MQ=255) ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat > 6:72679/1‑145 (MQ=255) ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat < 5:72679/148‑4 (MQ=255) tGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACGCCGTGACCTTCCCTCGCGGCAc > 2:510805/1‑275 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 8:50186/1‑149 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 5:135469/1‑149 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat > 6:186641/1‑149 (MQ=255) aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCCCgg > 2:446910/1‑274 (MQ=255) ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtctc < 8:416028/149‑1 (MQ=255) gcgcGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCa < 8:372736/149‑1 (MQ=255) gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt > 8:60195/1‑149 (MQ=255) gTTCGCGCAGTGGATAGTCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCNGAATATTNCCANCATCGANAAGGAt > 3:24497/1‑145 (MQ=255) gcgcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa < 1:24497/145‑1 (MQ=255) aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATc < 7:332774/149‑1 (MQ=255) gTGGATAGGCGACTTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAAGAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGcc < 2:395219/274‑1 (MQ=255) ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg > 5:348281/1‑149 (MQ=255) ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGcc > 4:162151/1‑197 (MQ=255) aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg < 5:186641/149‑1 (MQ=255) aGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt < 2:162153/197‑1 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt > 7:33318/1‑149 (MQ=255) gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt > 6:72181/1‑149 (MQ=255) cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt < 7:50186/149‑1 (MQ=255) gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc < 6:30196/109‑1 (MQ=255) gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa < 1:76239/85‑1 (MQ=255) gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAAGATTAGCCGAGAAGACACGATTGCCCCGCGTaaaccgt > 4:332175/1‑270 (MQ=255) gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACg > 8:143844/1‑87 (MQ=37) tGTCAAATACGTTCATATCAGTGAACAACGCCCAGGACTGAAAGAACATGCGCATCCTTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt < 2:322815/215‑1 (MQ=255) gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc < 7:212454/149‑1 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATCACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCCGCACGGTCAGGGAACTATTATCGACGATGCCGCGATTGCCACGTGTaaaaattaaatc > 1:39390/1‑267 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga < 8:80836/84‑1 (MQ=255) tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga > 7:80836/1‑84 (MQ=255) aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt < 5:402135/133‑1 (MQ=255) aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt > 6:402135/1‑133 (MQ=255) aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATc > 5:158925/1‑149 (MQ=255) tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg > 7:379309/1‑111 (MQ=255) tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg < 8:379309/111‑1 (MQ=255) tCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACTCGTAAAACTGACATCGCGCATATCg > 2:273296/1‑275 (MQ=255) tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGt > 5:384742/1‑149 (MQ=255) tCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAg > 8:267472/1‑149 (MQ=255) cgataacaacGCCCCAGACTGAAATAACATGCTCATCCGTTTGCGCACGGTATACAG‑GCGCTAACGGGACATCGCCGGAATATTCTCACCATCGAAAAGGTGCTCACCAGGATCTGGTGCGATTGGCCCGCCAAGCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAAt < 4:273292/271‑1 (MQ=255) aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg > 6:429885/1‑149 (MQ=255) gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt > 8:273549/1‑149 (MQ=255) tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc > 7:17042/1‑149 (MQ=255) gAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg < 6:232479/149‑1 (MQ=255) aacaacGCCCCGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt > 8:125793/1‑149 (MQ=255) aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCa > 1:83597/1‑169 (MQ=255) cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCCAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATAACGACTAAATTCGccccc > 2:9835/1‑271 (MQ=255) ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc < 1:14813/97‑1 (MQ=255) ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAt < 3:83597/169‑1 (MQ=255) cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa > 5:380537/1‑86 (MQ=255) ccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGAt < 7:108497/149‑1 (MQ=255) cGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa > 1:78891/1‑84 (MQ=255) caaCTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑TCCCGCACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGACGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCt < 2:514111/273‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc < 7:229791/145‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc > 8:229791/1‑145 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc < 5:453857/145‑1 (MQ=255) tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc > 6:453857/1‑145 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc > 5:51822/1‑64 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc < 6:51822/64‑1 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa < 5:281077/122‑1 (MQ=255) aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa > 6:281077/1‑122 (MQ=255) aTAACATGCTCCTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCTGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAAtt < 1:56522/275‑1 (MQ=255) cTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAAGGTCAGGGAAATCTTATCGAAGATGCAGCGATTGCCAAGCGTAAAACTGACATCGCGATTATAGAATCAATTCTCCACACCCTGCCACACAATTCACCATTc > 4:274836/1‑275 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg < 8:61186/92‑1 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg > 7:61186/1‑92 (MQ=255) aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc > 5:307493/1‑149 (MQ=255) gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg < 5:429885/149‑1 (MQ=255) ttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAt < 7:84713/149‑1 (MQ=255) gcACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGGCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTAAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAt < 2:53217/275‑1 (MQ=255) gcACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTc > 7:302990/1‑149 (MQ=255) tGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCg < 8:169630/82‑1 (MQ=255) tGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCg > 7:169630/1‑82 (MQ=255) gTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTcg > 6:98535/1‑149 (MQ=255) aCAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt < 2:45622/116‑1 (MQ=255) aG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAc < 6:348281/149‑1 (MQ=255) cgcgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc > 3:130860/1‑74 (MQ=255) cgcgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCAc > 1:25377/1‑184 (MQ=255) cgcgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTTCTCCAATAATCACCCCTCGTTTTGCGTTGCTTTTTTAAGccaggc > 4:189138/1‑270 (MQ=255) gcgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTc > 8:102005/1‑149 (MQ=255) cgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGNGGCACGGTCAGGGAAATCTTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAANATCGCCACAGCATGCCCCATAATTAACCCCNNCTCTTGCGTTGATTTTATAAATATGgctc > 3:63952/1‑273 (MQ=255) cgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACt > 2:213094/1‑211 (MQ=255) cgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg > 2:74407/1‑267 (MQ=255) cgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgcgc > 2:378497/1‑275 (MQ=255) cgAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGAGCATATCCACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTGTCTa > 2:42424/1‑265 (MQ=255) | CCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCT > NC_000913/3339576‑3340107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |