Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,676,271 A→T 5.9% intergenic (+163/+19) yhjE → / ← yhjG putative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,2710AT 5.9% 228.5 / 4.4 68intergenic (+163/+19)yhjE/yhjGputative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base A (34/30);  new base T (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

AACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGAATTCCTTTACTCTCCGGATCAATAGTCAAATCCAG  >  NC_000913/3676007‑3676538
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                           
aactaCGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCCACCCGCATCCGGCATGAACAAAGCACCCTTTGCCAACAATCTAAATCTTTTTACTTTCCGGCAGTCAGAAATcccga                                                                                                                                                                                                                                                                   >  4:447512/1‑272 (MQ=255)
            ggTGCGGTGGGGTTATATCTGGCGGGGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGACGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAAGTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatcat                                                                                                                                                                                                                                                          <  4:271612/272‑1 (MQ=255)
                           tatCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGGCATCCGGCAATTATCATCACTTCTTCATCTGCGa                                                                                                                                                                                                                                         >  2:47812/1‑274 (MQ=255)
                                        tggctggcTTTACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAAGAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGac                                                                                                                                                                                                                           <  1:67857/275‑1 (MQ=255)
                                                   aCGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACAGCAGTCGTTGTAATAAGAGTTAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGc                                                                                                                                                                                                                 <  2:135721/274‑1 (MQ=255)
                                                              ccTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCgg                                                                                                                                                                                                      <  2:447517/274‑1 (MQ=255)
                                                                            aTGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCACTTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAGCGTgaagaccgt                                                                                                                                                                                        >  4:319129/1‑267 (MQ=255)
                                                                                  cgcgACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTa                                                                                                                                                                                 >  1:38154/1‑275 (MQ=255)
                                                                                                                     tGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACTATCTAAATCTGTTTAATTGCCGGATGTCAg                                                                                                                                                                                                                                                                            >  5:309264/1‑149 (MQ=255)
                                                                                                                             tACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATc                                                                                                                                                                                                                                                                       <  7:451614/146‑1 (MQ=255)
                                                                                                                             tACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATc                                                                                                                                                                                                                                                                       >  8:451614/1‑146 (MQ=255)
                                                                                                                              cgggttgtCAGGCCGGAATTTCCATACAATATATTGATATCGCAAGGATTTGGAGGCCGGATAAGGCGTTCAAGCCGCATCCGGAATGAACAAGGCAAACTTGGCAGACAATCGAAATCGGTTTAATGGCCGTATGACAGCCATCCGGCAATTATCACCACTTCGTCATCTGCGACAAAATCGTCCGACACTGATGAGCCTCCGCTTCGGAAGGGGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACa                                                                                                                                     <  4:94993/268‑1 (MQ=255)
                                                                                                                                    aTCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACCGaacca                                                                                                                               >  1:99180/1‑275 (MQ=255)
                                                                                                                                     tCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatc                                                                                                                                                                                                                                                            <  6:309264/149‑1 (MQ=255)
                                                                                                                                            tGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCCACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCTACAAAATCGTCCGACACTGATTAGCCTACCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCACCGTTCCCAGTGCCGCAGCAACAGAACCACGGGCaa                                                                                                                        >  4:477038/1‑274 (MQ=255)
                                                                                                                                            tGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGc                                                                                                                                                                                                                                                                   <  7:68563/135‑1 (MQ=255)
                                                                                                                                            tGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGc                                                                                                                                                                                                                                                                   >  8:68563/1‑135 (MQ=255)
                                                                                                                                                tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAg                                                                                                                    <  2:477043/274‑1 (MQ=255)
                                                                                                                                                      tACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGc                                                                                                                                                                                                                                           <  6:49982/149‑1 (MQ=255)
                                                                                                                                                           tatatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTTAGATCAGTGCCAGTAACGCCGCCCCCGGTGTTACCAGCGTTGCCAGTGCCGCAACACCAGCACCCCGGGCAATACGCGGTCCGGCttc                                                                                                        >  4:363630/1‑274 (MQ=255)
                                                                                                                                                               ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATc                                                                                                                                                                                                                                  >  8:253223/1‑149 (MQ=255)
                                                                                                                                                                  aaTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCCATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACGGATTAGCCTCACCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCACCGCCGGTGTTACCAGCGTTGCCAGTGCCGCACCAACACCACCCCCGGCAATCAACGGTCCGGCTTTCACCCCa                                                                                                 >  4:239055/1‑275 (MQ=255)
                                                                                                                                                                   aTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTcc                                                                                                                                                                                                                              >  7:402261/1‑149 (MQ=255)
                                                                                                                                                                       gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCa                                                                                                                                                                                                                                                                             <  5:89552/98‑1 (MQ=255)
                                                                                                                                                                       gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCa                                                                                                                                                                                                                                                                             >  6:89552/1‑98 (MQ=255)
                                                                                                                                                                       gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCt                                                                                                                                                                                                          >  2:73211/1‑165 (MQ=255)
                                                                                                                                                                       gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgca                                                                                                                                           >  1:44308/1‑228 (MQ=255)
                                                                                                                                                                       gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCACCCTg                                                                                            >  1:99065/1‑275 (MQ=255)
                                                                                                                                                                        cAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGGATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGc                                                                                           <  2:324763/275‑1 (MQ=255)
                                                                                                                                                                           ggATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCgg                                                                                                                                                                                                      <  4:73210/165‑1 (MQ=255)
                                                                                                                                                                                  tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCt                                                                                                                                                                                                               <  7:110295/149‑1 (MQ=255)
                                                                                                                                                                                       cGGATAAGGCTTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCACTTATCATCACTTCTTTATCTGCGATAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCAGCCGTTGTTACCAGCGTTGCCAGTGCCGAAGCAACAGCAACACGGGCAATCAGCGGTCCGGCTTGACCACAAGCCTGGGGTTTTTTAAACGt                                                                             >  4:130277/1‑274 (MQ=255)
                                                                                                                                                                                       cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACCAAATCGTCCGACACTGATNAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCACGTGCCGCAGCAACAGNTCAACGGGCAATCACAGGTCCGGCGNTCACCCCAGACCCCGGAACTTTACACgg                                                                             >  3:97713/1‑273 (MQ=255)
                                                                                                                                                                                          aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACAAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCTGTCCGGCTTTCAACCCAGACTGCGGATTTTTAAACGTccc                                                                          >  2:26339/1‑274 (MQ=255)
                                                                                                                                                                                             agtgcttCACGCCGCAGCCGGCATGAACAAAGCACACTTTGCCACCAATCTAAAACTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGc                                                                      <  1:74203/270‑1 (MQ=255)
                                                                                                                                                                                               gCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGACGGTGAGACCAGTACCAGTAACGCCTCCGCCGGTGTTACCAGCGTTCCCAGTGCCCCATCAACGGCCCCCCGGGCAACCCACTGTCCGCCTTTCACCCCACCCTGCGGTCTTTTACACGTCCCCCgta                                                                     >  4:299769/1‑272 (MQ=255)
                                                                                                                                                                                                 gTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                                                                                                                                                >  6:399567/1‑149 (MQ=255)
                                                                                                                                                                                                  ttCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTga                                                                                                                                                                                               <  7:253223/149‑1 (MQ=255)
                                                                                                                                                                                                    cACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACa                                                               >  2:51924/1‑275 (MQ=255)
                                                                                                                                                                                                     aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCg                                                                                                                                                                                                                                                                     >  7:53939/1‑76 (MQ=255)
                                                                                                                                                                                                     aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCg                                                                                                                                                                                                                                                                     <  8:53939/76‑1 (MQ=255)
                                                                                                                                                                                                     aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACa                                                               <  2:319133/274‑1 (MQ=255)
                                                                                                                                                                                                      cgccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATc                                                                                                                                                                                           >  8:38601/1‑149 (MQ=255)
                                                                                                                                                                                                         cgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGt                                                                                                                                                                                        <  8:402261/149‑1 (MQ=255)
                                                                                                                                                                                                         cgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAgcggc                                                          >  2:308028/1‑275 (MQ=255)
                                                                                                                                                                                                              ccGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGaa                                                       <  2:51715/273‑1 (MQ=255)
                                                                                                                                                                                                              ccGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATCAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGaa                                                       <  2:493188/273‑1 (MQ=255)
                                                                                                                                                                                                               cGGCATGACCAAAGCACACTTTGCCAACCATCTAAATCTGTTTAAGTGCCGGATGTCAGACCTCCGGAAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACCGATTAGCCTCCCCTTCGGAAGGAGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACg                                                     <  4:51923/274‑1 (MQ=255)
                                                                                                                                                                                                               cGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACg                                                     <  2:363635/274‑1 (MQ=255)
                                                                                                                                                                                                                             caacatttGCAAACAACTTGAATTTGTGTAATGGCTGGACAGCAGACATCCCGCAATTATCATCACGTTGTCCTCTGCGAGAAAATCGGCCGACACTGATTAGCGTTCGCTTCGGAAGGTGAGATCAGGGACGGTGACGCCGCCGGCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACACCGGACTTCGGATTTTTAAGCGTCCCCCGCACATACAGCGGCGAACGAAGTGTGATAATCCg                                      <  4:65005/270‑1 (MQ=255)
                                                                                                                                                                                                                                acTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCg                                                                                                             >  2:480577/1‑201 (MQ=255)
                                                                                                                                                                                                                                acTTTGCCAAAAATCTAAATCGTTTTAATGTCCGGATTTCAGACAGCCGGCAATTATCACCACTTCTCCATCTGCGAAAAAATCGTCCGACACTCATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGaa                                    <  4:308024/274‑1 (MQ=255)
                                                                                                                                                                                                                                  tttGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCa                                                                                                                                                                                          <  7:64052/122‑1 (MQ=255)
                                                                                                                                                                                                                                  tttGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCa                                                                                                                                                                                          >  8:64052/1‑122 (MQ=255)
                                                                                                                                                                                                                                     gCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGAATTCCtt                              <  2:130278/275‑1 (MQ=255)
                                                                                                                                                                                                                                          caaCCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGtt                                                                                                                                                       >  7:147540/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aTCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCGTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGAATTCCTTTACtctc                       <  1:97713/275‑1 (MQ=255)
                                                                                                                                                                                                                                            aTCTAAATCTGTTTAATTGCCGGATGTAAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGAATTCCTTTACtctc                       <  2:13993/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   tCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGcc                                                                                                                                              >  6:458219/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     tGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCCCATCCAGCGGCGAACGCAGTGTGCTAACCCGAATTCCTTTACCCTCCGGacc                 >  4:3806/1‑270 (MQ=255)
                                                                                                                                                                                                                                                     tGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAgcgg                                                           >  2:501685/1‑230 (MQ=255)
                                                                                                                                                                                                                                                       tttAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAg                                                                                                                                                  >  7:159815/1‑141 (MQ=255)
                                                                                                                                                                                                                                                       tttAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAg                                                                                                                                                  <  8:159815/141‑1 (MQ=255)
                                                                                                                                                                                                                                                               ccGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGc                                                                                                                                                     >  4:480776/1‑130 (MQ=255)
                                                                                                                                                                                                                                                                cGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcac                                                                                                                                 >  6:27004/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 ggATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTCTCACCCCAGCCTGCGGAATTTTAACCGTCCCCCGCCCATACAGCGGCGAACGCAGTGTGTTAATCCGACTTCCTTACCCCTCCGGACCAAtggctaacaccac  >  4:238012/1‑263 (MQ=255)
                                                                                                                                                                                                                                                                   aTGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGt                                                                                                                                                 <  2:480781/130‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCa                                                   <  1:100119/222‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACggg                                                                                                                           >  5:320649/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       cAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGc                                                                                                                          <  7:38601/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                           
AACTACGGGCTAGGTGCGGTGGGGTTATATCTGGCGGCGATGGCTGGCTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAATCAGCGGTCCGGCTTTCACCCCAGCCTGCGGATTTTTAAACGTCCCCCGCACATACAGCGGCGAACGCAGTGTGATAATCCGAATTCCTTTACTCTCCGGATCAATAGTCAAATCCAG  >  NC_000913/3676007‑3676538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: