| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,056,539 | T→A | 6.1% | intergenic (‑200/+86) | glnL ← / ← glnA | sensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,056,539 | 0 | T | A | 6.1% | 507.8 / 18.6 | 149 | intergenic (‑200/+86) | glnL/glnA | sensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base T (72/68); new base A (3/6); total (75/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-04 | |||||||||||
Only 100 of 150 total aligned reads displayed.
AAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCA > NC_000913/4056279‑4056802 | ttAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAA‑GNAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGGGACTCACCTGCTCAGGCCTAANTCATAATCACCGGTTAGTCAGGGAANAGA‑TCATTTTCGGCGCAACCGGCGTGagt > 3:97444/1‑273 (MQ=255) ttAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTGTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTAACACAACATAACCTGGTAGCA‑CGGATAAGCCGCATTTGCGTCGCATCCGGCGTGAt > 4:349588/1‑274 (MQ=255) tAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCAACTCATCCCCGTGTAGGC‑CGGATAAGACGCATTTGCGTCCCACCCGGCGTGAtg > 4:397194/1‑274 (MQ=255) gCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTTCCACCGATCCCAGGcc > 2:363256/1‑275 (MQ=255) ggCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAACAAAGCCCATGCAGAGATGGGCTTCAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCAACCGATCCCGGGCCTGCCAGCGAc > 2:332711/1‑274 (MQ=255) tGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACCCCTTTGTGGCCCATCCGGGCTGATGTGCTTGCCACCCATCCCACGCCTCGCAGACACAGGCGaa > 4:65846/1‑274 (MQ=255) aatttggtataaggAAAAGGTTTCAACACTTTTATGGCATTATAATTTTTTACGGATAACAAGGCGGAAGAATCAGAGAATTGAC‑GGGGAAAAGAGCCCATGCAGGGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGc < 4:494530/262‑1 (MQ=255) tctcCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCCTAGACAGGCGCAAAGTTTCCACGGCAACTaaaa > 1:124825/1‑275 (MQ=255) tctcCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa < 2:349593/275‑1 (MQ=255) aaaGGTTGACCAA‑TTTTATGGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGAATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGAAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 4:363251/273‑1 (MQ=255) aaaGGTTGAACCA‑TTTTAGTGAATTATAGCTTTTTACGGATAAAAAGCGCAGAGCATCAGGGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 4:318228/273‑1 (MQ=255) aCAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 1:90437/275‑1 (MQ=255) aCAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 2:55075/275‑1 (MQ=255) gTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 2:471618/1‑267 (MQ=255) gTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCt > 1:121143/1‑275 (MQ=255) gTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCCCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCCGGGACAGGCGGAAAGTTTCCACGGCCACCaaacaccccgtctct > 4:165762/1‑262 (MQ=255) cACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGc < 2:165764/251‑1 (MQ=255) aCCA‑TTTTAGTGCATTATAGCTATCTAAGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGTCCATGCAGAGATGGGCTACAGCTAGCTGAGAAACTTCACGATAGAGAGCGAACCACATGAACCGGCTTTCCTCATAGTAACATTCTAGGA‑CTGCATAGCAGCATTCGAGTCGCACACGGTGCAATGAGCTTGCCACGTAGACAACGCAGGAATGTGACAGGAGATAAGTTTCCACGGCCACAAAACCAATTaacagccgatg > 4:429955/1‑264 (MQ=255) tAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTCTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATATGCTTGCCACCGAACCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAACt > 2:457226/1‑274 (MQ=255) gggCATTATGGATTTTTACGGATAAAAAGCGCGGAGCATCAGGGAATGGAA‑GAAGAAAAATGCCGATGCAGAGATGGGCTACAGATAGCTGGTAAACTTCACGTTGGAGGGCGACTCACCTGCTCCGGCCGACCACATCATCAGCGTGTAGGC‑CGGCTAAGACGCATGTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCa < 4:74650/272‑1 (MQ=255) tGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCt < 1:97444/271‑1 (MQ=255) cATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 2:304854/1‑250 (MQ=255) tataGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGAGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTACCAGAACCAGGCGAACAGTTTCCCAGGCCACTAAAACACTTAGAAGCTGAATTACATCTCAAAATCTa > 1:11216/1‑275 (MQ=255) aGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt < 4:104124/179‑1 (MQ=255) ttttACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtgtg > 3:77107/1‑178 (MQ=255) tACGGGTAAAAAGCACGAGGCATCAG‑GAAATGACAGGCGCAAAAGGCCCATGTAGGGATTGGCTGTAGATAGCTGAGAAACTTCACGTTGGGTAGCGACTCACCTGCGCCGGCCTACCGCATCAGCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCg < 4:459375/274‑1 (MQ=255) ggATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCAtt > 6:61845/1‑149 (MQ=255) aaGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCg > 7:201750/1‑149 (MQ=255) cgAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCg > 2:510626/1‑153 (MQ=255) cgAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGCTGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 4:303546/1‑222 (MQ=255) gAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATGCGACGCAAATGCGTcttatccg < 6:25563/149‑8 (MQ=255) aaGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATGCGACGCAAATGCGTCTTATCCgg < 7:374164/149‑1 (MQ=255) gCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt < 4:510621/153‑1 (MQ=255) gCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 2:303550/222‑1 (MQ=255) tCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATGCGACGCAAATGCGTcttatccg > 6:115742/1‑136 (MQ=255) tCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATGCGACGCAAATGCGTCTTATCCg < 5:115742/143‑1 (MQ=255) tCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGa > 7:17480/1‑149 (MQ=255) gaATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGc < 6:380133/149‑1 (MQ=255) aaTTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCt < 5:133797/149‑1 (MQ=255) atggAC‑GGAGAAAGAGACCCATGCAGGGAGGGACTAAAGATGGCTGACAAACTCCACGTTGGAGAGCGACTCCCCTNCTCCGGCCGACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGGCGCAGCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTcg < 3:121143/271‑1 (MQ=255) ggAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTc < 5:292323/123‑1 (MQ=255) gagaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCa > 4:509981/1‑125 (MQ=255) aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa < 2:231527/191‑1 (MQ=255) aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg < 8:288461/149‑1 (MQ=255) aGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACACCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAgg < 5:75669/149‑1 (MQ=255) cccATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTTTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATCTGCtt > 7:210393/1‑132 (MQ=255) cccATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCtt < 8:210393/132‑1 (MQ=255) agagATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 2:510279/188‑1 (MQ=255) agagATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCaa < 1:113011/206‑1 (MQ=255) aTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACa > 5:469159/1‑149 (MQ=255) ggCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGc > 7:451375/1‑149 (MQ=255) ggCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCtt > 2:273568/1‑274 (MQ=255) cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa < 5:325556/149‑1 (MQ=255) tACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTACGC‑CGGATGCGACGCAAATGCGTcttat > 6:196667/1‑95 (MQ=37) tACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTACGC‑CGGATGCGACGCAAATGCGTcttat < 5:196667/99‑5 (MQ=37) gATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGt < 8:17480/149‑1 (MQ=255) tAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGttt < 7:319680/149‑1 (MQ=255) aGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTc < 6:260444/149‑1 (MQ=255) cTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCa < 5:427461/149‑1 (MQ=255) tGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCAc > 7:238467/1‑149 (MQ=255) aCAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCCGTGAACACGcc > 2:438775/1‑275 (MQ=255) aCAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGcc > 1:3061/1‑275 (MQ=255) ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAgg < 5:378881/112‑1 (MQ=255) ttCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAgg > 6:378881/1‑112 (MQ=255) aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa < 5:243984/149‑1 (MQ=255) gACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCt < 8:417391/149‑1 (MQ=255) aCTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTg < 6:327941/149‑1 (MQ=255) ccTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagta > 7:367736/1‑149 (MQ=255) cTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg < 8:167264/89‑1 (MQ=255) cGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCa < 6:469159/149‑1 (MQ=255) ccTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAc < 8:367736/149‑1 (MQ=255) ccTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAc < 5:411156/149‑1 (MQ=255) cTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAct > 8:462987/1‑149 (MQ=255) cTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAct > 5:251672/1‑149 (MQ=255) aCCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGc > 6:181092/1‑140 (MQ=255) aCCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGc < 5:181092/140‑1 (MQ=255) aCCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAgttcgttc < 2:83003/275‑1 (MQ=255) ccACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 1:27149/1‑121 (MQ=255) ccACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTa > 7:326654/1‑149 (MQ=255) atcatcACCGAGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTATCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGAt < 2:58393/259‑1 (MQ=255) tcatcaCCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 2:206871/1‑116 (MQ=255) catcaCCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAAc < 6:418944/139‑1 (MQ=255) caCCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 4:206868/116‑1 (MQ=255) aCCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCAt > 2:434106/1‑199 (MQ=255) tgtAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacg > 4:322327/1‑154 (MQ=255) tgtAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCAtcttc > 4:298335/1‑169 (MQ=255) tgtAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCag > 3:17598/1‑181 (MQ=255) gtAGGC‑CGGATGAGACGCATTTGCGTAGCACCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCGGCCAGGGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGAACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCagag < 4:439002/275‑1 (MQ=255) gtAGGC‑CCGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGAGCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCagag < 2:85344/275‑1 (MQ=255) aGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATa < 8:222758/149‑1 (MQ=255) ggC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAGAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTcgcg < 4:54972/171‑1 (MQ=255) ggC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCAtgc < 8:326654/149‑3 (MQ=255) ggC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg < 2:322331/154‑1 (MQ=255) gC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACt > 2:168980/1‑102 (MQ=255) gATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa < 4:168978/102‑1 (MQ=255) aTAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCCGAGCGATGTACGCATCAATTGCTTCGTCAGTTAACACGCCACCGGCCTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCCGGGCTTCTTCCAGGGAGCCTGCa > 2:16010/1‑274 (MQ=255) gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc > 8:116977/1‑149 (MQ=255) gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc > 8:90139/1‑149 (MQ=255) gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCCACCGGATGCGGAGTCATACGCACGCGGTc > 7:122974/1‑149 (MQ=255) aTTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCAtctt > 6:318137/1‑149 (MQ=255) | AAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCA‑TTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGAC‑GGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGC‑CGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCA > NC_000913/4056279‑4056802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |