Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 443,022 G→A 9.1% intergenic (+25/+29) thiI → / ← yajL tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,0220GA 9.1% 189.5 / 9.1 66intergenic (+25/+29)thiI/yajLtRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base A (3/3);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-03

TCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAT  >  NC_000913/442759‑443281
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                    
tCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACANAACCTGGCTGCTGTGGTGTGAGCGCGGGGGGATGAGACGTCTGCAGGCGCGCTATCTGCGCGAGCAGGGCTTTANAAATGTGAAGGGAAATCGCGCGTATGTTGTNNGTTTTACGGAGCCTCTGGTAAGATGCTAc                                                                                                                                                                                                                                                           >  3:61793/1‑275 (MQ=255)
                   ccTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACact                                                                                                                                                                                                                                        >  2:251470/1‑275 (MQ=255)
                             ctgtCTATCGATGAAAAGGAAGAAAAGCCAAGGAAAGTAGAAGGGATTGATGTGTTTTCTCTGCCATTCAATAAAAGTAGCACCAAATTTGGCGTGATCGACCAGAACAAAACATGGCTGCTGTGGGGTGAGCGCGGGGTGATGAGCCGTCAGCAGGCGCACTATCTGCGCGAGCAGAGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                                                                                                                               <  4:251466/271‑1 (MQ=255)
                             cGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                                                                                                                               <  2:345843/274‑1 (MQ=255)
                                       aTGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCGATCTGCGCGAGCCGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGGTTTTACGTCCCATCTGGTTAAGTGCGTCGTTTGCCGCACTCGACACTACTCGCAAAAATTACAAAt                                                                                                                                                                                                                     >  4:345838/1‑274 (MQ=255)
                                         gAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGAGAGCAGGGCTATAACAATGTTAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATcac                                                                                                                                                                                                                  >  2:355737/1‑273 (MQ=255)
                                                     aaGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCATGCCGCCATCa                                                                                                                                                                                                       >  2:438412/1‑274 (MQ=255)
                                                                        ggATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACAAGAACAAAACCTGGCTGCTGTGGTGGGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                                                                                                                                  <  2:146880/275‑1 (MQ=255)
                                                                                                           aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTTAGCGCGGGGTGATGAGCAGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTACTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCGCTGCCTACATCACCAGTTGTGATGCCACTGCATGAGCTTTGTGCCGCCCAACCACACGGTCGATaa                                                                                                                                                >  4:425234/1‑274 (MQ=255)
                                                                                                           aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGAGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGACCGTAATTGGTGGTTTTTACGACGCATCTGGTCAGAGACGACGTTTGCCGCATCCGAACCTACCCGCACAAATGAGAAATACCAGCCGCCCGCACAGGTGTGGCTCCCCCTTCATGGGCATTTTCCGGCacaaccaaacgggcggt                                                                                                                                                  >  4:305772/1‑263 (MQ=255)
                                                                                                            gCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              >  2:184816/1‑275 (MQ=255)
                                                                                                                  aaTTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGa                                                                                                                                        <  1:61793/275‑1 (MQ=255)
                                                                                                                     ttGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTc                                                                                                                                                                                                                                                                    <  6:396038/149‑1 (MQ=255)
                                                                                                                         cGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAt                                                                                                                                                                                                                                                                <  8:24910/149‑1 (MQ=255)
                                                                                                                         cGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAt                                                                                                                                                                                                                                                                <  5:198478/149‑1 (MQ=255)
                                                                                                                           aTCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGc                                                                                                                                                                                                                                                              <  6:445136/149‑1 (MQ=255)
                                                                                                                            tctcGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCg                                                                                                                                                                                                                                                             <  8:208181/149‑1 (MQ=255)
                                                                                                                                       aCAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                                                                                                                                                                                           >  8:291938/1‑140 (MQ=255)
                                                                                                                                       aCAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                                                                                                                                                                                           <  7:291938/140‑1 (MQ=255)
                                                                                                                                                ggCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCAGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATTATTTTCAGACCAAAGTCGATAGCTGTACCCTGCCCctgg                                                                                                          >  2:169872/1‑275 (MQ=255)
                                                                                                                                                      tgtgGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACtt                                                                                                                                                                                    >  2:134326/1‑195 (MQ=255)
                                                                                                                                                          gtgtGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                                                                                                                                                                                <  4:134325/195‑1 (MQ=255)
                                                                                                                                                           tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                                                                                                                                                                                              >  7:18463/1‑149 (MQ=255)
                                                                                                                                                             tgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGc                                                                                                                                                                                                                                                     >  2:400685/1‑124 (MQ=255)
                                                                                                                                                               aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCg                                                                                                                                                                                                                                             >  1:49546/1‑130 (MQ=255)
                                                                                                                                                               aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATta                                                                                                                                                                                                                          <  6:227732/149‑1 (MQ=255)
                                                                                                                                                                 cgcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCa                                                                                                                                                                                                                                                 <  4:400680/124‑1 (MQ=255)
                                                                                                                                                                   cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGacac                                                                                                                                                                                                                                         <  3:49546/130‑1 (MQ=255)
                                                                                                                                                                                 gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAgac                                                                          >  2:241516/1‑274 (MQ=255)
                                                                                                                                                                                  tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtg                                                                                                                                                                                                >  3:83240/1‑156 (MQ=255)
                                                                                                                                                                                     gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCTTGCCGCCATCACCa                                                                                                                                                                                                    <  6:13616/149‑1 (MQ=255)
                                                                                                                                                                                      cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                                                                                                                                                                                           <  1:83240/156‑1 (MQ=255)
                                                                                                                                                                                          cgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                                                                                                                                                                                               <  8:344060/149‑1 (MQ=255)
                                                                                                                                                                                            ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTACGACACAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTCCCCGTGCATCCCAgacaccccactttt                                                               >  4:220850/1‑263 (MQ=255)
                                                                                                                                                                                             tctTTCGGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTGTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTc                                                              <  2:305776/274‑1 (MQ=255)
                                                                                                                                                                                                        gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                                                                                                                                                                                <  6:291065/149‑1 (MQ=255)
                                                                                                                                                                                                         aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAg                                                                                                                                  >  3:77889/1‑194 (MQ=255)
                                                                                                                                                                                                         aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                                                                                                                                                                                           >  1:91908/1‑136 (MQ=255)
                                                                                                                                                                                                             gggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                                                                                                                              <  1:77889/194‑1 (MQ=255)
                                                                                                                                                                                                             gggCTTTAACAATGTGAAGGTATATCGCCCGAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                                                                                                                                                                                       <  3:91908/136‑1 (MQ=255)
                                                                                                                                                                                                                         tgtgAAGGTATATCGCCCGTTATTTGTGTTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTTCATCCCAGACGACGCGCTTGTCCCGCCATTGTTCGGCGGGAATTTTGTCt                                  >  2:88413/1‑274 (MQ=255)
                                                                                                                                                                                                                         tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATTCCTGCCGCCATCACAAGTTGTGATGCCACTTCATGGGCCTTTTCACGCCCAACCAACCGGTCAATAATTTGCAGACCAAAGTCGAAAGCTGTACCCGCCCCCTGGCCGGCCACACATTTTACCAGTTCATCCCCCAACACGCCCTCTTCCAGCCCCTCTTcggcggcacatctttctt                                 >  4:180839/1‑263 (MQ=255)
                                                                                                                                                                                                                             aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCa                              <  2:220854/274‑1 (MQ=255)
                                                                                                                                                                                                                                gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATgg                                                                                                                                                                               >  8:315632/1‑126 (MQ=255)
                                                                                                                                                                                                                                gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATgg                                                                                                                                                                               <  7:315632/126‑1 (MQ=255)
                                                                                                                                                                                                                                   tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                       >  2:323419/1‑275 (MQ=255)
                                                                                                                                                                                                                                   tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                       >  2:393129/1‑275 (MQ=255)
                                                                                                                                                                                                                                     tCGCTCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGTCGTTTGCCGCATCCGACACTACTCGTAATAAATATAAATCCCTGCCGACATCACCAGTTGTGATGCCACTGCATGGGCTTTTTCACGGCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGGAATGTTGCCCGTGCATCCCAgacg                                                                         <  1:120515/223‑1 (MQ=255)
                                                                                                                                                                                                                                     tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgc                                                                                                                                                                                                            >  2:406598/1‑93 (MQ=255)
                                                                                                                                                                                                                                         ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATc                                                                                                                                                                                                        <  4:406593/93‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCa                                                                                                                                                                                                       <  6:185070/93‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCa                                                                                                                                                                                                       >  5:185070/1‑93 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGAATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGcc                 >  2:473155/1‑274 (MQ=255)
                                                                                                                                                                                                                                           gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACCACTCGTTATAATTATAAATCCCTTCCGCCATCACCAGTTGTGATGTCACTTCATGGGCTTTTGACCGCTCCACCAACAGGCCGATACTTTTCAGACCAACGTCGATAGCGATACCCGGCCCCTGGCTGGTCAGCACTTTTACCCGTGCATCCCAGCCCACCCCCTTGGCCAGACCTTGTTCGGGCGGAATTTTGGTTTTCAAGGTCCGGAACCCgg               >  4:249126/1‑275 (MQ=255)
                                                                                                                                                                                                                                             aaTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAGTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTc             >  2:268066/1‑275 (MQ=255)
                                                                                                                                                                                                                                               tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCg                <  2:249130/270‑1 (MQ=255)
                                                                                                                                                                                                                                                   tgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                                                                                                                       >  4:529966/1‑115 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                                                                                                                   <  2:529971/115‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              <  7:377087/136‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              >  8:377087/1‑136 (MQ=255)
                                                                                                                                                                                                                                                        tttACGTCGCGTCTGGTCCGATGCGCAGTTTGCCGCAGCCGACACTCCTCTTTATAATTATAAATCCCTGCCGCCATCACCATTTGTGATGCCACTTCAGGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAGAGCTGTACCCGGCCCCTGGCTGGGCAGCAATTTTACCCGTGCATCCCTGACGACGCGCTTGGCCAGCCATTGTTCGGCGGAAAGTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAt  <  4:268062/275‑1 (MQ=255)
                                                                                                                                                                                                                                                        tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGGGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAt  <  4:393124/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              tCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                                                                                                                             >  7:91788/1‑146 (MQ=255)
                                                                                                                                                                                                                                                              tCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                                                                                                                             <  8:91788/146‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aTCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCaaa                                                                                                                                   >  5:294455/1‑136 (MQ=255)
                                                                                                                                                                                                                                                                  aTCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCaaa                                                                                                                                   <  6:294455/136‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCCCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg                                                                                                                 >  6:388539/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                    
TCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAT  >  NC_000913/442759‑443281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: