Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 443,023 T→C 9.0% intergenic (+26/+28) thiI → / ← yajL tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,0230TC 9.0% 188.1 / 9.6 67intergenic (+26/+28)thiI/yajLtRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base C (3/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-03

TCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAT  >  NC_000913/442759‑443281
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                   
tCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACANAACCTGGCTGCTGTGGTGTGAGCGCGGGGGGATGAGACGTCTGCAGGCGCGCTATCTGCGCGAGCAGGGCTTTANAAATGTGAAGGGAAATCGCGCGTATGTTGTNNGTTTTACGGAGCCTCTGGTAAGATGCTAc                                                                                                                                                                                                                                                           >  3:61793/1‑275 (MQ=255)
                   ccTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACact                                                                                                                                                                                                                                        >  2:251470/1‑275 (MQ=255)
                             ctgtCTATCGATGAAAAGGAAGAAAAGCCAAGGAAAGTAGAAGGGATTGATGTGTTTTCTCTGCCATTCAATAAAAGTAGCACCAAATTTGGCGTGATCGACCAGAACAAAACATGGCTGCTGTGGGGTGAGCGCGGGGTGATGAGCCGTCAGCAGGCGCACTATCTGCGCGAGCAGAGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                                                                                                                               <  4:251466/271‑1 (MQ=255)
                             cGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                                                                                                                               <  2:345843/274‑1 (MQ=255)
                                       aTGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCGATCTGCGCGAGCCGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGGTTTTACGTCCCATCTGGTTAAGTGCGTCGTTTGCCGCACTCGACACTACTCGCAAAAATTACAAAt                                                                                                                                                                                                                     >  4:345838/1‑274 (MQ=255)
                                         gAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGAGAGCAGGGCTATAACAATGTTAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATcac                                                                                                                                                                                                                  >  2:355737/1‑273 (MQ=255)
                                                     aaGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCATGCCGCCATCa                                                                                                                                                                                                       >  2:438412/1‑274 (MQ=255)
                                                                        ggATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACAAGAACAAAACCTGGCTGCTGTGGTGGGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCa                                                                                                                                                                                  <  2:146880/275‑1 (MQ=255)
                                                                                                           aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTTAGCGCGGGGTGATGAGCAGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTACTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCGCTGCCTACATCACCAGTTGT‑GATGCCACTGCATGAGCTTTGTGCCGCCCAACCACACGGTCGATaa                                                                                                                                                >  4:425234/1‑274 (MQ=255)
                                                                                                           aGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGAGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGACCGTAATTGGTGGTTTTTACGACGCATCTGGTCAGAGACGACGTTTGCCGCATCCGAACCTACCCGCACAAATGAGAAATACCAGCCGCCCGCA‑CAGGTGTGGCTCCCCCTTCATGGGCATTTTCCGGCacaaccaaacgggcggt                                                                                                                                                  >  4:305772/1‑263 (MQ=255)
                                                                                                            gCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              >  2:184816/1‑275 (MQ=255)
                                                                                                                  aaTTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGa                                                                                                                                        <  1:61793/275‑1 (MQ=255)
                                                                                                                     ttGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTc                                                                                                                                                                                                                                                                    <  6:396038/149‑1 (MQ=255)
                                                                                                                         cGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAt                                                                                                                                                                                                                                                                <  8:24910/149‑1 (MQ=255)
                                                                                                                         cGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAt                                                                                                                                                                                                                                                                <  5:198478/149‑1 (MQ=255)
                                                                                                                           aTCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGc                                                                                                                                                                                                                                                              <  6:445136/149‑1 (MQ=255)
                                                                                                                            tctcGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCg                                                                                                                                                                                                                                                             <  8:208181/149‑1 (MQ=255)
                                                                                                                                       aCAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                                                                                                                                                                                           >  8:291938/1‑140 (MQ=255)
                                                                                                                                       aCAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                                                                                                                                                                                           <  7:291938/140‑1 (MQ=255)
                                                                                                                                                ggCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCAGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATTATTTTCAGACCAAAGTCGATAGCTGTACCCTGCCCctgg                                                                                                          >  2:169872/1‑275 (MQ=255)
                                                                                                                                                      tgtgGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACtt                                                                                                                                                                                    >  2:134326/1‑195 (MQ=255)
                                                                                                                                                          gtgtGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATg                                                                                                                                                                                <  4:134325/195‑1 (MQ=255)
                                                                                                                                                           tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                                                                                                                                                                                              >  7:18463/1‑149 (MQ=255)
                                                                                                                                                             tgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGc                                                                                                                                                                                                                                                     >  2:400685/1‑124 (MQ=255)
                                                                                                                                                               aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCg                                                                                                                                                                                                                                             >  1:49546/1‑130 (MQ=255)
                                                                                                                                                               aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATta                                                                                                                                                                                                                          <  6:227732/149‑1 (MQ=255)
                                                                                                                                                                 cgcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCa                                                                                                                                                                                                                                                 <  4:400680/124‑1 (MQ=255)
                                                                                                                                                                   cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGacac                                                                                                                                                                                                                                         <  3:49546/130‑1 (MQ=255)
                                                                                                                                                                                 gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAgac                                                                          >  2:241516/1‑274 (MQ=255)
                                                                                                                                                                                  tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtg                                                                                                                                                                                                >  3:83240/1‑156 (MQ=255)
                                                                                                                                                                                     gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCTTGCCGCCATCACCa                                                                                                                                                                                                    <  6:13616/149‑1 (MQ=255)
                                                                                                                                                                                      cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GAt                                                                                                                                                                                           <  1:83240/156‑1 (MQ=255)
                                                                                                                                                                                          cgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                                                                                                                                                                                               <  8:344060/149‑1 (MQ=255)
                                                                                                                                                                                            ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTACGACACAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTCCCCGTGCATCCCAgacaccccactttt                                                               >  4:220850/1‑263 (MQ=255)
                                                                                                                                                                                             tctTTCGGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTGTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTc                                                              <  2:305776/274‑1 (MQ=255)
                                                                                                                                                                                                        gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATg                                                                                                                                                                                <  6:291065/149‑1 (MQ=255)
                                                                                                                                                                                                         aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAg                                                                                                                                  >  3:77889/1‑194 (MQ=255)
                                                                                                                                                                                                         aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCG‑AATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GAt                                                                                                                                                                                           >  1:91908/1‑136 (MQ=255)
                                                                                                                                                                                                             gggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                                                                                                                              <  1:77889/194‑1 (MQ=255)
                                                                                                                                                                                                             gggCTTTAACAATGTGAAGGTATATCGCCCG‑AATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCa                                                                                                                                                                                       <  3:91908/136‑1 (MQ=255)
                                                                                                                                                                                                                         tgtgAAGGTATATCGCCCGTTATTTGTGTTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTTCATCCCAGACGACGCGCTTGTCCCGCCATTGTTCGGCGGGAATTTTGTCt                                  >  2:88413/1‑274 (MQ=255)
                                                                                                                                                                                                                         tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATTCCTGCCGCCATCACAAGTTGT‑GATGCCACTTCATGGGCCTTTTCACGCCCAACCAACCGGTCAATAATTTGCAGACCAAAGTCGAAAGCTGTACCCGCCCCCTGGCCGGCCACACATTTTACCAGTTCATCCCCCAACACGCCCTCTTCCAGCCCCTCTTcggcggcacatctttctt                                 >  4:180839/1‑263 (MQ=255)
                                                                                                                                                                                                                             aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCa                              <  2:220854/274‑1 (MQ=255)
                                                                                                                                                                                                                                gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATgg                                                                                                                                                                               >  8:315632/1‑126 (MQ=255)
                                                                                                                                                                                                                                gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATgg                                                                                                                                                                               <  7:315632/126‑1 (MQ=255)
                                                                                                                                                                                                                                   tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                       >  2:323419/1‑275 (MQ=255)
                                                                                                                                                                                                                                   tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCgg                       >  2:393129/1‑275 (MQ=255)
                                                                                                                                                                                                                                     tCGCTCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGTCGTTTGCCGCATCCGACACTACTCGTAATAAATATAAATCCCTGCCGACATCACCAGTTGT‑GATGCCACTGCATGGGCTTTTTCACGGCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGGAATGTTGCCCGTGCATCCCAgacg                                                                         <  1:120515/223‑1 (MQ=255)
                                                                                                                                                                                                                                     tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgc                                                                                                                                                                                                            >  2:406598/1‑93 (MQ=255)
                                                                                                                                                                                                                                         ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATc                                                                                                                                                                                                        <  4:406593/93‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCa                                                                                                                                                                                                       <  6:185070/93‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCa                                                                                                                                                                                                       >  5:185070/1‑93 (MQ=255)
                                                                                                                                                                                                                                          cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGAATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGcc                 >  2:473155/1‑274 (MQ=255)
                                                                                                                                                                                                                                           gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACCACTCGTTATAATTATAAATCCCTTCCGCCATCACCAGTTGT‑GATGTCACTTCATGGGCTTTTGACCGCTCCACCAACAGGCCGATACTTTTCAGACCAACGTCGATAGCGATACCCGGCCCCTGGCTGGTCAGCACTTTTACCCGTGCATCCCAGCCCACCCCCTTGGCCAGACCTTGTTCGGGCGGAATTTTGGTTTTCAAGGTCCGGAACCCgg               >  4:249126/1‑275 (MQ=255)
                                                                                                                                                                                                                                             aaTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAGTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTc             >  2:268066/1‑275 (MQ=255)
                                                                                                                                                                                                                                               tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCg                <  2:249130/270‑1 (MQ=255)
                                                                                                                                                                                                                                                   tgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTc                                                                                                                                                                       >  4:529966/1‑115 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGc                                                                                                                                                                   <  2:529971/115‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              <  7:377087/136‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                                                                                                                              >  8:377087/1‑136 (MQ=255)
                                                                                                                                                                                                                                                        tttACGTCGCGTCTGGTCCGATGCGCAGTTTGCCGCAGCCGACACTCCTCTTTATAATTATAAATCCCTGCCGCCATCACCATTTGT‑GATGCCACTTCAGGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAGAGCTGTACCCGGCCCCTGGCTGGGCAGCAATTTTACCCGTGCATCCCTGACGACGCGCTTGGCCAGCCATTGTTCGGCGGAAAGTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAt  <  4:268062/275‑1 (MQ=255)
                                                                                                                                                                                                                                                        tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGG‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAt  <  4:393124/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              tCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                                                                                                                             >  7:91788/1‑146 (MQ=255)
                                                                                                                                                                                                                                                              tCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                                                                                                                             <  8:91788/146‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aTCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCaaa                                                                                                                                   >  5:294455/1‑136 (MQ=255)
                                                                                                                                                                                                                                                                  aTCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCaaa                                                                                                                                   <  6:294455/136‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCCCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg                                                                                                                 >  6:388539/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                   
TCGGCCCGAACGACGTGATCCTCGATATCCGTTCTATCGATGAACAGGAAGATAAGCCACTGAAAGTCGAAGGGATTGATGTGGTTTCTCTGCCGTTCTATAAACTGAGCACCAAATTTGGCGATCTCGACCAGAACAAAACCTGGCTGCTGTGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGT‑GATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCATTGTTCGGCGGGAATTTTGTCTTTCAGCGTCGGGAAGCCGGTCATATTACCAAT  >  NC_000913/442759‑443281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑