| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,422,895 | C→G | 6.0% | A17G (GCC→GGC) | ulaE → | L‑xylulose 5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,422,895 | 0 | C | G | 6.0% | 307.6 / 7.3 | 90 | A17G (GCC→GGC) | ulaE | L‑xylulose 5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (42/42); new base G (2/4); total (44/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01 | |||||||||||
TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGT > NC_000913/4422662‑4423158 | ttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGt < 2:158903/275‑1 (MQ=255) ggttatGGGTTTCACAGGCAACGTAAGCGGAGGCTGGGCGGTGGATGGACGGCTGGGGATCAAGGGGATTCCGAGTCACGTCGTAACCGGGGTCGGTAGTAGCGGGGATGACGCTTCTCCGGTGGAAGCCGCACGTCGGTTCAAACGTTCCATCGCGGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTa < 4:468441/271‑1 (MQ=255) caccgtcaccgGAGGCATGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTAGCCGTGATGCCGCCTCTCCGGTGGAAGCCGCACGTCAGTACAAACGTTCCATCGCTTAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTTGCATCTTTGAAAAAGCGCTCTCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTGTGTCGtaa > 1:97899/1‑275 (MQ=255) ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCaa > 2:357157/1‑275 (MQ=255) tttcgaaTTTACGTCTTTATGTCGGGCCGGAGTATCCGGGATGCCGCTTCTCGNGTGGNAGCCGCACGTCAGTTCAAACGTTNCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGtt < 4:504030/200‑1 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCgggg > 3:43089/1‑168 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCGGTCGCGCCCCAACTGGAGCcgc > 1:106938/1‑275 (MQ=255) ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACGGACGATCGCCTGTCGCCCCTCAACTGGAGCcgc > 2:231102/1‑275 (MQ=255) ggATTCACGTCGTTATCGCGAGGCCTAGTATGCGTGGTGGCTGTGCTCCGGTGGAAGACGCACGGCAGTGCAAACCTTACATTGAGGAAGTGGGGGGGTAGGGAGCGGAGATTTTGTCCAAAGAAATCGTGGTAGACAGCGATGAAATATCGCTCCCCGCGGTGGAGTGGTGGCAGAAACGCCTGCAACTGGCAAAAACGTTAGGCATAGATGTAGGCGAAATGTCGGTAGATGAAGCTGACGAGCGCCTGTCGCGCCTCAACTGGAGCCGCGa < 4:4554/273‑1 (MQ=255) gATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTg < 1:43089/168‑1 (MQ=255) gTCTTTACTGCGGGCCGTAGTTTCCGTGAGGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGTGCGGATATGTTGTCCAAACAAATCCCGCTTAGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGTAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc < 2:341841/274‑1 (MQ=255) tttATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc > 7:391082/1‑149 (MQ=255) taactgggggCCGTAGTATACGGGATGGCGCTTTTCCGTTGGAGGCCGTACGTCGGTTCAAACGTTCCATCGCCGAACTGGGGGGCTAAGGAGCGGAGAGGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCGTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg < 4:207075/268‑1 (MQ=255) cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAg > 8:463105/1‑149 (MQ=255) ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAAc > 2:140394/1‑179 (MQ=255) gCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa > 1:45740/1‑274 (MQ=255) ccGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgct < 5:189479/149‑1 (MQ=255) gtagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTa < 4:140393/179‑1 (MQ=255) gtagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCACTCCCCGGCGGGGAGCgcttt < 7:278672/149‑3 (MQ=255) tagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc > 5:452462/1‑149 (MQ=255) agtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct < 5:119258/149‑1 (MQ=255) gtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg < 7:23676/149‑1 (MQ=255) taTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg > 5:242773/1‑149 (MQ=255) acacgggagtccGCGTGTCCGGGGGAAGCCGCACGTCAGGTCAACCGTTCCGTCGCTGAAAGGGGGGGCAAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttgt < 4:352911/265‑1 (MQ=255) tCCGTGATGCCGCTTCTCCGGTGGCAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc > 4:527374/1‑144 (MQ=255) tGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa < 2:527379/144‑1 (MQ=255) ggTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCaaaaa > 6:107167/1‑149 (MQ=255) gAAGCCACACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGtt < 7:42999/149‑1 (MQ=255) aGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGa < 7:467541/111‑1 (MQ=255) aGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGa > 8:467541/1‑111 (MQ=255) gCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCtt < 6:234976/149‑1 (MQ=255) gTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAt > 5:236313/1‑149 (MQ=255) gTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTg < 6:242773/149‑1 (MQ=255) ttCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAttt < 7:225460/146‑1 (MQ=255) ttCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAttt > 8:225460/1‑146 (MQ=255) ttCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGt > 5:449479/1‑149 (MQ=255) ttCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGt < 5:107167/149‑1 (MQ=255) aaaCGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAtt < 5:435604/142‑1 (MQ=255) aaaCGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAtt > 6:435604/1‑142 (MQ=255) ttCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTa < 8:5457/127‑1 (MQ=255) ttCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTa > 7:5457/1‑127 (MQ=255) gCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGa > 7:73949/1‑149 (MQ=255) aaCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCt > 2:76378/1‑274 (MQ=255) aCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa < 6:236313/149‑1 (MQ=255) tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa < 8:73949/149‑1 (MQ=255) tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa < 8:259657/149‑1 (MQ=255) cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGGGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCGGGGAAg > 1:13140/1‑275 (MQ=255) gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTg > 6:336327/1‑149 (MQ=255) aGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcg < 2:328170/275‑1 (MQ=255) cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAcgc‑g > 2:433149/1‑275 (MQ=255) cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCGGCTCATCTTCGTTTCTCGCTGGGGCGTGCAGATGAcgc‑g > 2:227880/1‑275 (MQ=255) cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGAAACGCCTGCAACTGTCAAAAACGTTAGGCTTCGATTTTGTCGACATGTCGGTCGATGAAGCTGACGATCGCCTGTCGCGCCCCAACTGGAGCACCGGGCAGCGTCTGGCGCTGGTCGCTGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCCACGTCGTTTCCCGGTGGGCAGTGAATATGAccag > 4:313221/1‑272 (MQ=255) tatGTTGTCCAAACACATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGTAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTg < 2:313225/274‑1 (MQ=255) tgtggtCCAACCAAAGCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCg < 2:210931/274‑1 (MQ=255) gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCt > 2:161115/1‑249 (MQ=255) gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCt < 4:161113/249‑1 (MQ=255) ttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCaa > 8:270109/1‑149 (MQ=255) gtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGGTTGTTGAAACCGGCATGCACGTTCCGTCCCTGTGCCTTTCTTGCCCGCGTCGTTTCCCGCTGGGGCGTGAAAATGGCCCGGGTGTGGgcg > 4:371463/1‑275 (MQ=255) gtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCCCAACTGGAGCCGCCagnag > 4:550889/1‑163 (MQ=255) ggcaaaacaaaTCCCGGTTGGCTACCATGAAAACGGGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACTTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGgcgc < 2:142028/271‑1 (MQ=255) tCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTg > 5:188280/1‑149 (MQ=255) caaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACt > 7:120382/1‑146 (MQ=255) caaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACt < 8:120382/146‑1 (MQ=255) aaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg > 8:292367/1‑149 (MQ=255) aaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc < 2:550894/163‑1 (MQ=255) aaacaaaTCCCGCTTGGCATCTATGAAAAAGCACTCCCCGGCGGGGAGCgct < 8:83095/52‑1 (MQ=25) aaacaaaTCCCGCTTGGCATCTATGAAAAAGCACTCCCCGGCGGGGAGCgct > 7:83095/1‑52 (MQ=25) aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt > 2:72614/1‑235 (MQ=255) acaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGcc > 5:110966/1‑149 (MQ=255) caaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGc < 2:466829/274‑1 (MQ=255) aaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCg < 4:72613/235‑1 (MQ=255) tCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag < 7:270109/149‑1 (MQ=255) ccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagca > 8:422681/1‑149 (MQ=255) cGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGAtt < 2:301045/275‑1 (MQ=255) ggtgggCATTTATGAAAAGGCGCTCCGNGNGGGGGGGGGCTGGCTGGAACGCTGGCAACTGGCAAAAACGTTAGGNTGCGATTTTGGCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGNAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGAtt < 3:45740/270‑1 (MQ=255) cTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTTAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGGCCGTTCCCGCTGGGCAGTGAAGAAGACGC‑GGTGCGGGCTGAGGGGCTgggagatag > 4:301041/1‑268 (MQ=255) cTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCTTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGAAGTGAAGATGACGC‑GGTGCGGGCGAAGGGGCTGGAGATTAt > 2:285540/1‑275 (MQ=255) ggCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCg < 2:63808/275‑1 (MQ=255) agaagAAAAAGCCCTCCCCGGCGGGGGGTGTGGGCGGGAACGCCTGAAACTTGCAAAAACGTTAGGCTTAGATTTTGNGAAACTTTGGGTGGTTGAAGCTGGCGTTCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGTGCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAg < 3:106938/271‑1 (MQ=255) aTGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg < 6:449479/149‑1 (MQ=255) gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTAt < 2:504915/275‑1 (MQ=255) aaaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTg < 7:47755/121‑1 (MQ=255) aaaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTg > 8:47755/1‑121 (MQ=255) aaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGt > 5:456296/1‑56 (MQ=255) aaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGt < 6:456296/56‑1 (MQ=255) aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCaa > 5:385004/1‑149 (MQ=255) caGCACTCCCCGGCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt < 6:353542/135‑1 (MQ=255) caGCACTCCCCGGCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt > 5:353542/2‑136 (MQ=255) aGCTCTCCCCGCTGGGTAGTGCTGGCTGGAACGCCGGCAACTGGCAAAAACGTTAGGCGTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGTAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGt < 1:13178/275‑1 (MQ=255) cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAAt > 3:65318/1‑144 (MQ=255) tCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCt < 7:101316/97‑1 (MQ=255) tCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCt > 8:101316/1‑97 (MQ=255) ccgccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa < 1:65318/144‑1 (MQ=255) ccgccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt < 6:404822/146‑1 (MQ=255) ccgccgGGGAGTGCTGGCTGGAACACCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt > 5:404822/1‑146 (MQ=255) ccgGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaa > 5:287214/1‑149 (MQ=255) | TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGC‑GGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGT > NC_000913/4422662‑4423158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |