Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 770,682 A→C 6.3% intergenic (+71/‑776) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913770,6820AC 6.3% 216.6 / 5.4 63intergenic (+71/‑776)mngB/cydAalpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base A (25/34);  new base C (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

ATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGT  >  NC_000913/770423‑770845
                                                                                                                                                                                                                                                                   |                                                                                                                                                                   
aTTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGAGCTTTGTTCATGCCGGATACGGCATGAACGCgtt                                                                                                                                                      >  2:261885/1‑275 (MQ=255)
   gtACGGGTGTTGAATACGGCTGAATCAGCAACCTGTGATTCGACTGTTGCTTTCAGTGGGGAGGTGATTTCCTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTGCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAtt                                                                                                                                                   <  4:499519/274‑1 (MQ=255)
    tACGGCTGTGTAATCCGGCTGAATCAGAAACCGGTGATGCGACTGTGGCGTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATTATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAtt                                                                                                                                                   <  4:320438/274‑1 (MQ=255)
     aCGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGATTGCAGTCGGAGGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAAGCTATCAGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCg                                                                                                                                                 <  1:139072/275‑1 (MQ=255)
     aCGGCTGGTTAATCCGTCTGAATCAGCAAACTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAAAACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGAGGCCTTATTTACCCGGCCAGTCACGGACGTTCAGTGACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCg                                                                                                                                                 <  2:204393/275‑1 (MQ=255)
            tttAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACa                                                                                                                                          >  2:320442/1‑275 (MQ=255)
                                     gtgATGCGACGGTTGCTTATAGTTGGAACGTGATTTCTTGCTCGGAAACGAGTATGGATGAAAACATTACCACCGAGGAAAATCAAGGTTCAAATATATCGTGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTg                                                                                                                  <  4:242437/274‑1 (MQ=255)
                                         tGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag                                                                                                             <  1:85747/275‑1 (MQ=255)
                                                                     aTTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGtctgt                                                                                  >  1:154606/1‑270 (MQ=255)
                                                                                                 aaCACATTACCACCGAGGAAAATCAAGGTTCAAGTCTATCGGGGTCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCGACAAAAGCAGGCAAATCCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCa                                                       <  2:92347/273‑1 (MQ=255)
                                                                                                       ttACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAg                                               <  2:197259/275‑1 (MQ=255)
                                                                                                        tACCACCGATGAAAATCAATGTTCAATTCTTCCGTGGCCTTTTTTCCCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGc                                              <  2:455050/275‑1 (MQ=255)
                                                                                                            accGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag                                                                                                             <  2:355132/208‑1 (MQ=255)
                                                                                                              cGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGAAACTGGCAAGCGTAAGGCATCCGGTAATTTTTCGTTTATCTTTCCTCTCAAGcccagta                                        >  2:485842/1‑270 (MQ=255)
                                                                                                               gAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATa                                                                                                                                                                     <  5:384713/149‑1 (MQ=255)
                                                                                                                   aaaaTCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGc                                                                                                                                                                 <  6:471866/149‑1 (MQ=255)
                                                                                                                    aaaTCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCa                                                                                                                                                                >  7:239868/1‑149 (MQ=255)
                                                                                                                       acAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCt                               <  2:126783/274‑1 (MQ=255)
                                                                                                                             ttCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCt                                                                                                                                                        <  6:76397/148‑1 (MQ=255)
                                                                                                                             ttCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCt                                                                                                                                                        >  5:76397/1‑148 (MQ=255)
                                                                                                                               cAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTa                                                                                                                                                     <  5:323991/149‑1 (MQ=255)
                                                                                                                                aaaTCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAt                                                                                                                                                    >  7:98097/1‑149 (MQ=255)
                                                                                                                                 aaTCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAtt                                                                                                                                                   >  7:385316/1‑149 (MQ=255)
                                                                                                                                  atcgttCGGGGGCTTGTTTACCCGGCCAATCAGGGACGTTCTGTTACCGGCTTGCCTTAATAACAAGTAAACCGAAGCCACAGATGCGCGGCCAGATTGTGGACAAAGGGCGCTTTGTTCATGCTGGATACGTCATGAACGCTTTATTCAGTATACAAAAGCAGGCAAGTTCAATATATTGCAGAGATGATGTAGTCACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAAc                    <  4:261881/270‑1 (MQ=255)
                                                                                                                                             ccTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa                                                                                                                                                            <  6:9308/128‑1 (MQ=255)
                                                                                                                                             ccTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa                                                                                                                                                            >  5:9308/1‑128 (MQ=255)
                                                                                                                                             ccTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaaaa                                                                                                                                       <  8:239868/149‑1 (MQ=255)
                                                                                                                                               ttttttACCCGGTCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa                                                                                                                                                            <  5:43000/126‑1 (MQ=255)
                                                                                                                                               ttttttACCCGGTCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCATGaa                                                                                                                                                            >  6:43000/1‑126 (MQ=255)
                                                                                                                                               ttttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTAc                                                                                                                                           <  8:280466/143‑1 (MQ=255)
                                                                                                                                               ttttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTAc                                                                                                                                           >  7:280466/1‑143 (MQ=255)
                                                                                                                                               ttttttACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGc                                                                                                                                     >  7:34211/1‑149 (MQ=255)
                                                                                                                                                    tACCCGGCCAGTCACGGCCGTTCAGTTACCGGCTTGCCTGAATAGCAATCTAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGt  <  1:16309/275‑1 (MQ=255)
                                                                                                                                                           ccAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa                                                                                                                         <  8:385316/149‑1 (MQ=255)
                                                                                                                                                               tCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatata                                                                                                                     <  5:97161/149‑1 (MQ=255)
                                                                                                                                                                    gACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCaaa                                                                                                                              >  2:106088/1‑135 (MQ=255)
                                                                                                                                                                    gACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCa                                                                                                                <  8:34211/149‑1 (MQ=255)
                                                                                                                                                                        ttCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCa                                                                                                                          <  4:106087/135‑1 (MQ=255)
                                                                                                                                                                         tCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTa                      >  2:367908/1‑234 (MQ=255)
                                                                                                                                                                          cAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgatg                                                                                                          <  5:49972/149‑1 (MQ=255)
                                                                                                                                                                             ttACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaa                  <  4:367903/234‑1 (MQ=255)
                                                                                                                                                                                     ttGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTTGCTTTATTTTTAAc                    >  1:151613/1‑224 (MQ=255)
                                                                                                                                                                                         cTGAAAAGCAATCAAACCGAAGCCACATATTGGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGGCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaaaa                <  3:151613/224‑1 (MQ=255)
                                                                                                                                                                                               aGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTa                                                                                     >  6:310319/1‑149 (MQ=255)
                                                                                                                                                                                                gCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAg                                                                                    <  5:310319/149‑1 (MQ=255)
                                                                                                                                                                                                   aTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg                                                                                                                                  <  7:353884/100‑1 (MQ=255)
                                                                                                                                                                                                   aTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAgg                                                                                                                                  >  8:353884/1‑100 (MQ=255)
                                                                                                                                                                                                    tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGatgatg                                                                                                       <  6:129001/126‑1 (MQ=255)
                                                                                                                                                                                                    tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGatgatg                                                                                                       >  5:129001/1‑126 (MQ=255)
                                                                                                                                                                                                        aCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAg                                                                            >  7:143732/1‑149 (MQ=255)
                                                                                                                                                                                                               ccACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAAttt                                                                     >  7:239727/1‑149 (MQ=255)
                                                                                                                                                                                                                               aGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatatat                                                                                                                    >  6:365139/1‑86 (MQ=255)
                                                                                                                                                                                                                               aGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAatatat                                                                                                                    <  5:365139/86‑1 (MQ=255)
                                                                                                                                                                                                                                       gACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGcc                                             <  8:98097/149‑1 (MQ=255)
                                                                                                                                                                                                                                        aCAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCa                                            >  8:137137/1‑149 (MQ=255)
                                                                                                                                                                                                                                           aaGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtgt                                   >  1:79692/1‑155 (MQ=255)
                                                                                                                                                                                                                                               gcgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCt                               <  3:79692/155‑1 (MQ=255)
                                                                                                                                                                                                                                                 gcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGc                                                                                                                                 <  2:231166/55‑1 (MQ=255)
                                                                                                                                                                                                                                                  cTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCagag                                                                                                             >  7:421740/1‑74 (MQ=255)
                                                                                                                                                                                                                                                  cTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCaaag                                                                                                             <  8:421740/74‑1 (MQ=255)
                                                                                                                                                                                                                                                        tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                            <  8:143732/149‑3 (MQ=255)
                                                                                                                                                                                                                                                           tGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttgagag                         >  7:154924/1‑144 (MQ=255)
                                                                                                                                                                                                                                                             ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttttt                       >  8:256502/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 aTACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaaa                 >  4:151699/1‑151 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                   
ATTTTACGGCTGTTTAATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCTTGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGT  >  NC_000913/770423‑770845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: