Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 887,392 | T→A | 5.2% | intergenic (‑53/‑31) | ybjJ ← / → rcdA | putative drug efflux MFS transporter, inner membrane protein/transcriptional regulator of csgD and ybiJI; autoregulator |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 887,392 | 0 | T | A | 5.2% | 331.0 / 7.9 | 96 | intergenic (‑53/‑31) | ybjJ/rcdA | putative drug efflux MFS transporter, inner membrane protein/transcriptional regulator of csgD and ybiJI; autoregulator |
Reads supporting (aligned to +/- strand): ref base T (55/36); new base A (3/2); total (58/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-03 |
GCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTG > NC_000913/887121‑887660 | gCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTg < 4:159445/275‑1 (MQ=255) gTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTa < 2:11856/275‑1 (MQ=255) gCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCAAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagag > 2:90112/1‑274 (MQ=255) aTCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATCCGTcgc > 2:129862/1‑274 (MQ=255) cGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCt > 2:438795/1‑275 (MQ=255) agagAACACCGCCCATTTCAGCGATCGAGACAGAGAGAAGATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCgaga < 2:157492/275‑1 (MQ=255) tCAGCGATTGGGAAAGAGAGAAAATCGCGGATAGAAGGGGTACGGGTTGCACAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGAATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACAAAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGc < 4:90111/273‑1 (MQ=255) aGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTNATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTg > 3:68459/1‑207 (MQ=255) aTCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCaga < 1:68459/207‑1 (MQ=255) cgcgGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTCCg > 4:284237/1‑274 (MQ=255) gcgGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAACTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGa > 2:58790/1‑275 (MQ=255) aaGCAGGCGGACGGGTTGCCCAGGACGCCAATAACAGGCCTGGAAAAAAGAAGAACATACACAGCGCCCAGGTTCGGCGGTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACa < 1:12711/274‑1 (MQ=255) aGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACa < 2:463179/274‑1 (MQ=255) aGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATg < 2:284241/273‑1 (MQ=255) cccAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAttt < 8:382995/149‑1 (MQ=255) cccAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagaga > 2:104010/1‑168 (MQ=255) aGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTa > 7:93231/1‑149 (MQ=255) aGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTa > 6:181850/1‑149 (MQ=255) ggACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGaa < 4:104009/168‑1 (MQ=255) gCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAAATGTACACaa < 8:319335/149‑1 (MQ=255) ccATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAAt > 6:235552/1‑149 (MQ=255) cATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGACAAAATTATCAACGCCCCGCTGGAGGCTGTGCACCTTTCCGGAATACATGCTGTTAC‑CACACCCCAAATTGCTAc > 4:228935/1‑274 (MQ=255) aTTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTg > 7:4806/1‑149 (MQ=255) tAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCa < 6:357566/149‑1 (MQ=255) tAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCa < 6:364772/149‑1 (MQ=255) aaCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATAGCag > 7:75751/1‑149 (MQ=255) aCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAAtt > 6:237940/1‑144 (MQ=255) aCAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAAtt < 5:237940/144‑1 (MQ=255) aGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagaga < 6:202616/149‑1 (MQ=255) aGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCaga > 6:247113/1‑147 (MQ=255) aGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCaga < 5:247113/147‑1 (MQ=255) tGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTACCGATACGCAACGGCGAGAACAAATTAACCAGGCCACGCGGGAGGCGGTGAAACTTTACGGAATACCTGCTGTTACGCAC‑CGCAAATTTGACACCCTTGCCGGGGTa > 4:371899/1‑275 (MQ=255) aaaaTAAGAACATAAACCGTGCCCAGGGTGTGCGTTTCAATGCACTACGAGAAGAATGTACGGTCATTGAAAACGTCAAAATAAGAAAAGGGAAGACAACATTATCACGGTAGTGTACATTTGTACACAATTGCAGAGAGATGAAATGa < 8:75751/149‑1 (MQ=255) aagaagAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACa < 5:181850/149‑1 (MQ=255) gaaCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGt > 5:409160/1‑149 (MQ=255) aaCATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTc < 5:456460/149‑1 (MQ=255) aTAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCggatcg > 6:190124/1‑144 (MQ=255) aaaCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCt > 8:220231/1‑149 (MQ=255) aaaCAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGAcc > 2:225734/1‑275 (MQ=255) cgcCCAGGTTCGGCGTTTCAATGCATTCCGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGTAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCATAGAGAGGAAATGCCATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCATGCCACGCTGGAGGCGGTGAAACTTTACGGAATa > 2:157391/1‑209 (MQ=255) cccAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATc > 4:354711/1‑171 (MQ=255) cccAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTAttt > 2:66041/1‑275 (MQ=255) ggTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAgg < 2:354716/171‑1 (MQ=255) cGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGc < 6:111031/149‑1 (MQ=255) gCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGa > 2:30458/1‑272 (MQ=255) gCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGACtt > 2:45134/1‑275 (MQ=255) cGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATGGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCNAAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGTCGAAACTTTAGGGAATATATGCTGTTACGCAC‑CGNAAAATTGCTAACCTGCCCGGGGTACCGTTTNNACCGGTGACCTACACttttttcggca > 3:58033/1‑266 (MQ=255) tCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACAATTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGaaaa > 8:421579/1‑149 (MQ=255) aaTGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGaaaaaa > 6:225089/1‑149 (MQ=255) aTTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATc < 5:235552/149‑1 (MQ=255) ttACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATcc > 6:37622/1‑149 (MQ=255) gTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGc < 8:93231/149‑1 (MQ=255) tGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGcc > 5:43657/1‑149 (MQ=255) agaaTTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTAcc > 2:402935/1‑220 (MQ=255) tttACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTg < 4:402930/220‑1 (MQ=255) acgggtaTAGATAAAGTAAAAATAAGAAAAGGGAAAGGAACAGTAGCAAGGTGTGGGAAATTTGAACAGAATTGGAGAGAGAGGAAATGACAAGCGTCGCGCTAACGATCCGCAACGGCGGGAAAAAATTATCCAGTCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTcag < 4:225730/267‑1 (MQ=255) cGGTCATAGATCACGTCAAAAGAAGAAGAGGGAAGAAAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTcagcag < 2:228939/275‑1 (MQ=255) cGGTCATAGATAACGTCAAAAGAAGAAAGGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACAAAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTcagcag < 4:66040/275‑1 (MQ=255) aggTCATAGATTACGTCAAAATAAGAAGAGGGATAACAACAGTAGCAAGGTTGTGTACATTTGTACAAAATTGCAGAGAGAGGAAATGACTGGCGCCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACTCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTcagcag < 4:210544/274‑1 (MQ=255) tCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGt < 7:220231/149‑1 (MQ=255) aTAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGCGGCGTTCAGCAGtttt > 2:267535/1‑274 (MQ=255) tAGATCACGTAAAAATAAGAAGAGGGAAGACAAAATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTa < 1:58033/274‑1 (MQ=255) gATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAAc > 7:462607/1‑149 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCag > 1:124555/1‑63 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAAt > 2:142898/1‑115 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGaa > 1:80209/1‑153 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACTGCCTTTTACGCAC‑CGCAAATTTCCTACCCTTGCCGGGGTACCGTTGGGCTGGA‑GACCATCCTTTTTTCAGGGAATTGATGTGTTTTTAATGGAGCGTTTCAGCATTTTTCCTgaattc > 4:289852/1‑271 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGAc > 2:236407/1‑214 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTca > 2:210547/1‑258 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATc > 2:54115/1‑275 (MQ=255) cGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATc > 2:280866/1‑275 (MQ=255) gTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACg > 8:167598/1‑149 (MQ=255) cAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCag > 1:132220/1‑60 (MQ=255) aaaaTAAGAAGAGGTAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagaggg < 3:124555/63‑3 (MQ=255) aaaaTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATc < 4:142897/115‑1 (MQ=255) aaaaTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACa < 3:80209/153‑1 (MQ=255) aaaaTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACctac < 4:236403/214‑1 (MQ=255) aaaaTAAGAAAAGGGAAGACAACATTAGCTAGGTTGTGTACATTTGTACACAATTGCAGAAGGATGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAGAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAA‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACt < 2:33164/265‑1 (MQ=255) aTAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagag < 3:132220/60‑1 (MQ=255) gaagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGc > 6:29951/1‑149 (MQ=255) gaagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTATACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTCACGTTGGGATCNATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGGGGCTTTCAGAAGTTTTACTGATATAATGTCACGGCa > 3:108959/1‑275 (MQ=255) aagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGaa > 8:318650/1‑130 (MQ=255) aagaGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGaa < 7:318650/130‑1 (MQ=255) aaGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGc > 5:83518/1‑149 (MQ=255) acaacaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTa > 2:203488/1‑223 (MQ=255) acaacaTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGTGGCGTTCAGCAGTTTTACTGAGATAATGTCCCGGCTATAGCGGGcct > 4:275821/1‑273 (MQ=255) caTTAGCACGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGAGTCGCGCTAACGATCCACAACGGCGAGAAAAAATTATCCAGGCTACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCaa > 6:454555/1‑149 (MQ=255) caTTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTgg < 4:203485/223‑1 (MQ=255) tgcaattGTGTACAAATGTACACAACTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGc > 6:434378/6‑149 (MQ=255) aaGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCt > 6:256249/1‑149 (MQ=255) ggTTGTGTACAATTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGTGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTAc < 7:421579/149‑1 (MQ=255) gTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTAcc > 8:335186/1‑149 (MQ=255) gTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCAAGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAA‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACATACTATTCTTCA‑AGAATTGATGAGTGGTTACTGGAGGCGTCAAGCAGTTTTACTGAGATCATGGCCCGGCACATCCAGGCATTTTTTAGCCATGtt > 4:197784/1‑275 (MQ=255) ttGTGTACAAATGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTAccc > 8:412211/1‑149 (MQ=255) tgtACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTg > 2:441164/1‑275 (MQ=255) tgtACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATAGCAGGCATTTTTTAGCGATTTTAGTg > 2:497857/1‑275 (MQ=255) aTTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCggg > 8:39429/1‑149 (MQ=255) | GCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGAGGAAATGACATGCGTCGCGCTAACGATCCGCAACGGCGAGAAAAAATTATCCAGGCCACGCTGGAGGCGGTGAAACTTTACGGAATACATGCTGTTACGCAC‑CGCAAAATTGCTACCCTTGCCGGGGTACCGTTGGGATCGATGACCTACTATTTTTCA‑GGAATTGATGAGTTGTTACTGGAGGCGTTCAGCAGTTTTACTGAGATCATGTCCCGGCAATATCAGGCATTTTTTAGCGATGTTAGTG > NC_000913/887121‑887660 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |