Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 998,451 T→A 6.8% intergenic (+44/‑39) elfA → / → elfD laminin‑binding fimbrin subunit/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913998,4510TA 6.8% 196.8 / 2.9 59intergenic (+44/‑39)elfA/elfDlaminin‑binding fimbrin subunit/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (30/25);  new base A (2/2);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CCTGCAAATGTATATGAATGATGGTACAACGGCCATCACGCCAGACACAGAAACCGGGAACATTTTGTTGCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGGTGTACGTAACACCAGTCCGGATGTTCCTTATCTTATTCAGTCATGGGTGATGACCCC  >  NC_000913/998230‑998698
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       
ccTGCAAATGTATATGAATGATGGTACAACGGCCATCACGCCAGACACAGAAACCGGGAACATTTTGTTGCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCTTa                                                                                                                                                                                                    >  2:3958/1‑275 (MQ=255)
                  tgataGTACAACGTCCATCACGCCAGAAACAGAAACCGGGAATATTTTGTTGCAGGGTGGAGCACAGTCTTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTTCGGTAAGTTGAATGAGGTAACAAATTTCCATATCAACTATTATTAATAGGACTGATTCATTCTTTTATTAATTAATACCCCTACAGAGCTCGGGAGGGTCTATTCATATCTTTTAAGTGTACCTATTTATGCACACTTGCATTACAAAGGGAATTGTGagc                                                                                                                                                                                  >  4:94810/1‑273 (MQ=255)
                              ggCCATCACGCCCGACACAGAAATCGGGAACATTTTGTTGCAGGATGGAGATCAGACGTTGACGTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTTAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTTTTTTATTAATTAGTACCCCTCCAGGGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAAc                                                                                                                                                                       <  4:341727/274‑1 (MQ=255)
                                                                               gaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAg                                                                                                                                                                                                                                                   <  7:69665/149‑1 (MQ=255)
                                                                                 tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                                                                                                                                 <  6:473772/149‑1 (MQ=255)
                                                                                  cAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                                                                                                                                <  5:392954/149‑1 (MQ=255)
                                                                                   aGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGa                                                                                                                                                                                                                                               >  8:56973/1‑149 (MQ=255)
                                                                                   aGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGa                                                                                                                                                                                                                                               <  5:76427/149‑1 (MQ=255)
                                                                                     aCGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGata                                                                                                                                                                                                                                             >  7:55663/1‑149 (MQ=255)
                                                                                          gACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCat                                                                                                                                                                                                                                        >  5:325623/1‑149 (MQ=255)
                                                                                             ttttAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                                                                                                                                     >  7:365743/1‑149 (MQ=255)
                                                                                             ttttAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                                                                                                                                     >  8:345142/1‑149 (MQ=255)
                                                                                                tAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAttttt                                                                                                                                                                                                                                  <  8:365743/149‑1 (MQ=255)
                                                                                                  aaGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTa                                                                                                                                                                                                                                >  7:75744/1‑149 (MQ=255)
                                                                                                    gTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAag                                                                                                                                                                                                                              >  8:330310/1‑149 (MQ=255)
                                                                                                       gATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGt                                                                                                                                                                                                                           <  5:186914/149‑1 (MQ=255)
                                                                                                        aTTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGtt                                                                                                                                                                                                                                                         >  2:195266/1‑118 (MQ=255)
                                                                                                        aTTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTg                                                                                                                                                                                                                          >  5:336215/1‑149 (MQ=255)
                                                                                                            tatCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTgg                                                                                                                                                                                                                                                     <  4:195264/118‑1 (MQ=255)
                                                                                                            tatCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTa                                                                                                                                                                                                                      <  7:56973/149‑1 (MQ=255)
                                                                                                                                   cAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACaaa                                                                                                                                                                                               <  8:75744/149‑1 (MQ=255)
                                                                                                                                          tgtgAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAAtt                                                                                                                                                                                        >  8:46370/1‑149 (MQ=255)
                                                                                                                                             gAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATtgtg                                                                                                                                                                                     <  8:164533/149‑1 (MQ=255)
                                                                                                                                                          aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTaa                                                                                                                                                                        <  7:330310/149‑1 (MQ=255)
                                                                                                                                                            tttCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACg                                                                                                                                                                      >  5:74464/1‑149 (MQ=255)
                                                                                                                                                               ccAAATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCTGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTAGGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTGATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGGTGTACGTAACACCAGTCCGGAt                                     <  2:94811/275‑1 (MQ=255)
                                                                                                                                                                    ttAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATAtt                                                                                                                                                              >  8:236698/1‑149 (MQ=255)
                                                                                                                                                                         tattatTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCa                                                                                                                                                         >  5:115468/1‑149 (MQ=255)
                                                                                                                                                                           ttattaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgc                                                                                                                                                       <  7:46370/149‑1 (MQ=255)
                                                                                                                                                                             attaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAAc                                                                                                                                                                                                           >  5:187911/1‑95 (MQ=255)
                                                                                                                                                                             attaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAAc                                                                                                                                                                                                           <  6:187911/95‑1 (MQ=255)
                                                                                                                                                                              ttaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgctgc                                                                                                                                                    <  6:336215/149‑1 (MQ=255)
                                                                                                                                                                              ttaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAg                                                                                                                                                        <  6:42588/145‑1 (MQ=255)
                                                                                                                                                                              ttaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAg                                                                                                                                                        >  5:42588/1‑145 (MQ=255)
                                                                                                                                                                                   aGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGGTGTACGTAACACCAGTCCGGATGTTCCTTATCTTATTCAGt                  >  2:13607/1‑274 (MQ=255)
                                                                                                                                                                                     aagtatttAATTTTTTTATTAATTGGTAGCCATCAAGGTGTCTGGAGGTGATATTCTGATTTTTGAAGAGTTACTATTTATTAAAACGTGCATCACAAAGGGAATTGTGACCGTGAGTTTAACTGCAATATGACTCAGCTGCTCATCCGCATGGTCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGGTGTACGTAACACCAGTCCGGATGTTCCTTATCTTATTCAGTCAt               <  4:3958/269‑1 (MQ=255)
                                                                                                                                                                                           ttAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATggg                                                                                                                                       >  6:225246/1‑149 (MQ=255)
                                                                                                                                                                                              aTTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGggcgg                                                                                                                                    >  6:462049/1‑149 (MQ=255)
                                                                                                                                                                                                  ttttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAgg                                                                                                                                >  6:387947/1‑149 (MQ=255)
                                                                                                                                                                                                  ttttATTAATTAGTACCACTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATAGTGACCGTAAGTTTAACGGAAAGATTCCTCAGCTGCTAATCAGCATGGGCGTCAGGTAAAGGAGGAATAGGACTTGCATAAAAAAGTCATGTGTCTTAAGCAGGTGCAGAGCAAATTTAAATGGGTGTACGTAACACCAGTCCGGATGTTCATTTACTTTTTCACTAGTGGGTGATGAcccc  >  4:307501/1‑275 (MQ=255)
                                                                                                                                                                                                    ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTCTGAAAACTTGCATAACAAAGGGAATTGTTACCGTAAGTTNAACGGCAATATTACTCAgctgcc                                                                                                                                                   >  3:33544/1‑127 (MQ=255)
                                                                                                                                                                                                    ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTaa                                                                                                                                                                              >  6:133288/1‑101 (MQ=255)
                                                                                                                                                                                                    ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTaa                                                                                                                                                                              <  5:133288/101‑1 (MQ=255)
                                                                                                                                                                                                    ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTa                                                                                                                              >  8:5832/1‑149 (MQ=255)
                                                                                                                                                                                                      attaattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTaaa                                                                                                                            >  5:17845/1‑149 (MQ=255)
                                                                                                                                                                                                        taattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATc                                                                                                                                               <  1:33544/128‑1 (MQ=255)
                                                                                                                                                                                                           ttaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCgg                                                                                                                       <  8:55663/149‑1 (MQ=255)
                                                                                                                                                                                                              gTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt                                                                                                                    >  7:141049/1‑149 (MQ=255)
                                                                                                                                                                                                               tACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt                                                                                                                   >  7:348218/1‑149 (MQ=255)
                                                                                                                                                                                                                tccccTCCAGAGCACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTg                                                                                                                  >  5:250079/2‑149 (MQ=255)
                                                                                                                                                                                                                tccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTg                                                                                                                  >  6:10281/2‑149 (MQ=255)
                                                                                                                                                                                                                  cccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa                                                                                                                <  6:325623/149‑1 (MQ=255)
                                                                                                                                                                                                                    cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACt                                                                                                              <  7:345142/149‑1 (MQ=255)
                                                                                                                                                                                                                    cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAACttt                                                                  >  4:209465/1‑193 (MQ=255)
                                                                                                                                                                                                                    cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACt                                                              >  3:132702/1‑197 (MQ=255)
                                                                                                                                                                                                                    cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGT‑AAGGCGGGATTGGACTTgca                                                                                                          >  1:25327/1‑152 (MQ=255)
                                                                                                                                                                                                                        aGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgca                                                                                                          <  6:115468/149‑1 (MQ=255)
                                                                                                                                                                                                                        aGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACt                                                              <  2:209468/193‑1 (MQ=255)
                                                                                                                                                                                                                        aGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGgt                                                          <  1:132702/197‑1 (MQ=255)
                                                                                                                                                                                                                        aGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGT‑AAGGCGGGATTGGACTTGCAGCaa                                                                                                      <  3:25327/152‑1 (MQ=255)
                                                                                                                                                                                                                            ttCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTg                                                                                                                  >  4:550639/1‑137 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       
CCTGCAAATGTATATGAATGATGGTACAACGGCCATCACGCCAGACACAGAAACCGGGAACATTTTGTTGCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATTCAGAAGGTGAAGAGCAAATTTCACTGGGTGTACGTAACACCAGTCCGGATGTTCCTTATCTTATTCAGTCATGGGTGATGACCCC  >  NC_000913/998230‑998698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑