| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,147,549 | C→A | 6.1% | intergenic (+9/‑72) | rpmF → / → plsX | 50S ribosomal subunit protein L32/putative phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,147,549 | 0 | C | A | 6.1% | 321.3 / 9.6 | 102 | intergenic (+9/‑72) | rpmF/plsX | 50S ribosomal subunit protein L32/putative phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (33/61); new base A (2/4); total (36/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAG > NC_000913/1147281‑1147822 | cATTTGGCGGATTACCAAGCTTAAAGCGTAAGTAATTGGTGCCCCCCTTTGGACCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAACCCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCACGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCg < 1:74442/273‑1 (MQ=255) tttGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtgat < 2:240553/275‑1 (MQ=255) taagtaaTTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCACCGCTACGTAATCCCGCATCTGCGTGATGACGCTTAGTGAGGTTTTTCCCCAg > 1:69309/1‑274 (MQ=255) aagtaaTTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTAGCATGCGTCGTTCCCATGACGCCCCTACCGCAGTCACCCGCCTGTCGTTAGCCCAAACTTCTGGTTAACAACACCTACGTACCCACATAACTTCCGACGGTTACTACCCCCGCCGCAAGGTCCTCGCTAAGTCATCACCCATTCGCGTGCTTACGCTTCGCGAGGATGTTACCCAgc > 4:40627/1‑273 (MQ=255) aagtaaTTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCAGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCCGTCACCAGCCTGTCTGTAGAAAAAACTTCGGGTGAAAAACATCTGCGTCACCCCCTCACTGCCGCCGGTTACTACCGCGGCTGCACGGGCATCGTTAACAAATCACGACACTGCAGTATGAATCTTAGGGAGTAttgcacctgggc > 4:382515/1‑265 (MQ=255) agtaaTTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAgg < 2:308364/273‑1 (MQ=255) cTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATAACGCGCTCACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGACAAACACCTGCGTCACCACACCACTGACGACGTGTACTACCGCGGACGCAAGGTCATCGCTAAGTAATCACCCTTCTGCGTCATGAACCTTAGTCACGTTTTTCCCCAGGAAAGTGGGGaaa > 4:218945/1‑274 (MQ=255) ccccGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCCCCCGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGCTAATACCCCCGCCGCAAGGTCACCGCTAAGTAATACAGCATCTGCGTGTTGAAGCTTAGTGAAGATTTTCCACAGGCAAACAGGGAAAgcc > 4:22792/1‑273 (MQ=255) gggATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCCACCGTAAGTAATCACGCATCTGCGTTGTGAACCTTAGTGAGGATTTTCCCCAGGCAAATGGGGAAAGCCCAAACCgggggg > 4:407635/1‑274 (MQ=255) aTAAACCGTAATTGAGGAGTAAGGTCCATGGCCATACAACCGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgac < 2:382520/275‑1 (MQ=255) tAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAACCCGGGCGGCGACGATAcc > 2:178020/1‑273 (MQ=255) aTTGAGGTGTAAGGTCCATGCCCGTAAAACAGAATAACCCAACCCGTTCCAAACTGGGCATGCCTCGTTCCCATGACGCGCTGACCGCACTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGa < 1:120163/275‑1 (MQ=255) ttaaggagTAAGGTCACTGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCAAGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGa < 1:145794/271‑1 (MQ=255) ttGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGa < 2:218948/274‑1 (MQ=255) gagTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGa > 2:115057/1‑214 (MQ=255) aaGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGAt < 4:115056/214‑1 (MQ=255) gtataagaaatagcaaaaGAAGAAACCAACTCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCGGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTG‑GGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt < 2:69100/261‑1 (MQ=255) ttcATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGAAAAAACTGCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg < 2:259231/273‑1 (MQ=255) ggagtgacaaacgAATAACCCAACCCGGTCCAAACGTCGCAAGGTGGGTTCCATTGACGCGTCAACCGCATTAGTCAGTGTGTATGATGACAAAATGTCTGGTGAAAAACACCTGTGGCACCAAGTGACTGCCGACCGTGAGTACCGCGGACGAAATGTCATCTCTAAGTAATCACGCATCTGCGGGATGAAGACTAGTGAGGATTTTCCCGAGGAAACTGGGGAAAGACAAAACCGGGTGGCGACGATACCTTGACACGTCTAACCCTGGCG‑t < 4:470552/262‑1 (MQ=255) ggCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑tt > 2:288808/1‑275 (MQ=255) ccGTAAAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTTCTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTa < 2:396716/274‑1 (MQ=255) tACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCCCGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGAAACGTCCaaa > 4:241561/1‑260 (MQ=255) acaGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTg < 2:241565/261‑1 (MQ=255) cctgTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCaatt > 4:388654/4‑195 (MQ=255) cccGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAACACTTCTGGTGAAAAACACCTGCGGCACCACATCACTGCCGTCGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGCAGCTTAGTGAGGATTTTTCCCAGGCCACTGGGGAACGACCAAACCGGGCGGCGCCGAGACCTTGAC‑CCTCTCAACCTCGCGTTTACATGTATAGGGATGGGTTTTTg > 4:442296/1‑275 (MQ=255) ttCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCTTGCTGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAACGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGCTGTCATGGGAGGGGATTTTGGcc > 2:201315/1‑274 (MQ=255) ttCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgggg < 2:388659/197‑1 (MQ=255) gTGGCAGGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTTTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGa < 2:22792/275‑1 (MQ=255) ggCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCCTCGCTAAGTAATCACGCATCTGCGTGATGAAGCtt > 4:151195/1‑159 (MQ=255) gagaTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTATGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCAACACATCACTGCCGACGGTTACTACCGCGGACGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACa < 2:461328/272‑1 (MQ=255) cATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga < 6:76448/149‑1 (MQ=255) cATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGtga < 8:101390/149‑1 (MQ=255) aTGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgat < 5:345556/149‑1 (MQ=255) tGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTg < 2:151196/159‑1 (MQ=255) gCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgatta < 7:334892/149‑3 (MQ=255) gCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgatta > 8:334892/1‑147 (MQ=255) gtcgtTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgattact < 7:238972/149‑5 (MQ=255) ttCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTa < 5:99225/149‑1 (MQ=255) cATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGa < 6:386793/149‑1 (MQ=255) gACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGa < 6:60042/149‑1 (MQ=255) cggctccccGCGGTCACCACCCTTTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCATCCCCACATCCCTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGATAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCa < 1:61740/268‑1 (MQ=255) cgcTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGAtttt < 6:384574/149‑1 (MQ=255) ccGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAg < 8:391003/149‑1 (MQ=255) ccGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAg < 8:42281/149‑1 (MQ=255) ccGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAg < 6:245593/149‑1 (MQ=255) gTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAAc < 8:104561/149‑1 (MQ=255) tCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACt < 6:225300/149‑1 (MQ=255) cAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTgggg > 5:244211/1‑149 (MQ=255) ccTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATc > 2:303301/1‑103 (MQ=255) ccTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaaa < 7:33276/149‑1 (MQ=255) ccTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaaa < 6:444661/149‑1 (MQ=255) ccTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGATCATCGCTAAGCAATCACGCATTTGCGTGATGAAGCTTAGTGAGGATTTTCCCAAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCGGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAact < 2:269815/275‑1 (MQ=255) gtctgtAGACAGAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGAc < 8:49015/149‑1 (MQ=255) tctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCg < 4:303297/103‑1 (MQ=255) tctgtAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAgg < 1:144845/133‑1 (MQ=255) gACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCg < 7:425873/149‑1 (MQ=255) gACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAACTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGACGGGCTTTTGTCCCTTCCGTGACAGTGCCTGCCGCATTCCCGGCACTTAATTCTCATTCCCAacctccccttct > 4:115351/1‑264 (MQ=255) aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgatta > 5:226316/1‑98 (MQ=255) aaaaCTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCAGATGCGtgatta < 6:226316/100‑3 (MQ=255) ttCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCg < 8:436197/141‑1 (MQ=255) ttCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCg > 7:436197/1‑141 (MQ=255) gTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGcgacg > 4:533324/1‑146 (MQ=255) tGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAc > 8:348631/1‑149 (MQ=255) gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaaa < 7:123150/124‑1 (MQ=255) gAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGaaa > 8:123150/1‑124 (MQ=255) aaaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCt > 6:94696/1‑149 (MQ=255) aaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATAc < 2:533329/146‑1 (MQ=255) aaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCtt < 6:157840/149‑1 (MQ=255) aaaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCtt < 6:244211/149‑1 (MQ=255) aaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTccc > 5:34147/1‑105 (MQ=255) aaaCACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTccc < 6:34147/105‑1 (MQ=255) ccTGCGTCAACACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGGTTAGTGGGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCAt < 2:442301/275‑1 (MQ=255) cTGCTTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATc < 2:363525/275‑1 (MQ=255) cTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGt > 5:463749/1‑149 (MQ=255) accacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAAccc > 7:278457/1‑149 (MQ=255) ccacATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCt < 6:402745/149‑1 (MQ=255) caTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGc > 7:355332/1‑149 (MQ=255) caTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGc < 8:355332/149‑1 (MQ=255) caTCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGc < 5:460545/149‑1 (MQ=255) gCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGAt < 6:390893/149‑1 (MQ=255) tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTcccc < 5:247521/70‑1 (MQ=255) tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTcccc > 6:247521/1‑70 (MQ=255) tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCt < 8:446853/111‑1 (MQ=255) tacCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCt > 7:446853/1‑111 (MQ=255) gcgGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGAtt < 7:348631/149‑1 (MQ=255) cgGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGAGAAAGACCAAACCGCGCGGCGACGATACTTTGACACGTCTAACCCTAGCG‑GTAGATGTCATGGGAGGGGAGTTTGGCTCTTCCGTGACCGGACCTGCAGCAGTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCTGCAATTCAGATGCCGTCACGCCCATACTTCCCAAACCTgctttttactaact > 4:174635/1‑262 (MQ=255) gCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGcc < 5:104136/149‑1 (MQ=255) tCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCg < 6:463749/149‑1 (MQ=255) cGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGt > 2:534853/1‑146 (MQ=255) tAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGt < 6:406948/149‑1 (MQ=255) tAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGt > 5:406948/1‑149 (MQ=255) aaGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACa < 4:534848/146‑1 (MQ=255) cGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTAGGGAAAGTCCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑CTAGATGTCATGGGAGGGGATTTTGTCCCTTCCGTGACAGTCCCTGCAGCATTgga > 1:16728/1‑152 (MQ=255) tcATCTGCGTGATGAAGGTTAGTGAGGATTTTCACCAGGCAACTGGGGAAAGACCAACCCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGcagt < 2:275344/274‑1 (MQ=255) gCATCTGCGTGATGAATCTTAGTGGGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCGTGACACGTCTAACCCTAGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGcagt < 2:174637/275‑1 (MQ=255) gCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTACCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGcagt < 2:350155/275‑1 (MQ=255) tCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAAtt > 3:110510/1‑167 (MQ=255) tCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACCCCCCCTTCTTTTAGTCGGCAATTCCCACGCCATCCCGCCATTCCTTGCCAACGCTTACTTTAAAACACTTTCCCGTCCCCAGATTTTTCCttcccctccct > 4:3388/1‑265 (MQ=255) cTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCa < 5:108530/149‑1 (MQ=255) cTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCa < 6:309950/149‑1 (MQ=255) | CATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTCACCAGCCTGTCTGTAGACAAAACTTCTGGTGAAAAACACCTGCGTCACCACATCACTGCCGACGGTTACTACCGCGGCCGCAAGGTCATCGCTAAGTAATCACGCATCTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCG‑TTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAG > NC_000913/1147281‑1147822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |