BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,147,987	G→C	6.1%	E123Q (GAG→CAG)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,987	0	G	C	6.1%	451.2 / 15.3	131	E123Q (GAG→CAG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand): ref base G (61/62); new base C (4/4); total (65/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 134 total aligned reads displayed.

CGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCGGTGCTTAAAACAATCCCTTCTATCAA  >  NC_000913/1147715‑1148249

cGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAg                                                                                                                                                                                                                                                                       >  2:448460/1‑275 (MQ=255)

cGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATAACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCTCGTGCCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCGGTGTCAGTGCCGGAGATACCGGGGCGCTGCTGGGGCTGGCAAACTTATTACCCACGCCCCGGGa                                                                                                                                                                                                                                                                        >  4:68958/1‑274 (MQ=255)

               ttttAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGCGGGAGTTCAATGCGTGTGGCCCTGGTGCTGGTGAAAGAAGGTCGAGCGCAAGCCTTTGTCCGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCCAACTTATTACTCAATCCCCTCGAGGGGCTTGAGCTTCCg                                                                                                                                                                                                                                                        >  4:413193/1‑275 (MQ=255)

                       ggCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGt                                                                                                                                                                                                                                                <  2:372264/275‑1 (MQ=255)

                        gCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTg                                                                                                                                                                                                                                               <  2:413198/275‑1 (MQ=255)

                        gCAATTCCGACGCCACCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCCCTGGTg                                                                                                                                                                                                                                               >  2:100157/1‑275 (MQ=255)

                         cAATTCCGACGCCATCCCGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTGGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGGTGCCCGGCCTTCGCAAGCAATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑a                                                                                                                                                                                                                                             <  2:289724/275‑1 (MQ=255)

                                                          agttGACTTTGGAAAACGTTCGCGGTTGCAGATTTTACCCGCGCATTCAGTTATCGCCAGTGAGGACCGGCCTTCGCAGGCGTTCCGCGCCAGTCGTGGAAGTTAAATGCGCGTGGCCCTGGCGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                                                                                                                                            <  4:53616/272‑1 (MQ=255)

                                                          aGCTGACTTTGAACAACGTTCTCGTCTGCAGATTATTCCTGCGAAGGCAGTTATCGCCAGTGAGGCCCGGCCTTCGCAAGCTATACGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                                                                                                                                            <  4:448455/275‑1 (MQ=255)

                                                           cagtacttgtaacaacGTTCGCGTCTGCAGATGATGCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGACAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                                                                                                                                            <  2:392837/264‑1 (MQ=255)

                                                                         acGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTCTCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGAGCACGCCTGTGTCAGTGCCGGTAATCCCGGGTCGCTGCTGGGGCTGGCACAAGTATTCCTCAAGCACCTGGGGGGGCTTAAACGTCAGGCGCGGTTG‑GCGGTCGTACCCCCTCCGCAAAAGGGACAAACGGGGGTCCTTGaactt                                                                                                                                                                                              >  4:392832/1‑270 (MQ=255)

                                                                                      cAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCAGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑CCGGTATTACCACATCAGCAACAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCgt                                                                                                                                                                                >  2:116041/1‑274 (MQ=255)

                                                                                                   cgcAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCt                                                                                                                                                                                                                                                   >  4:84358/1‑196 (MQ=255)

                                                                                                   cgcAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGcaca                                                                                                                                                                   >  2:79460/1‑275 (MQ=255)

                                                                                                       gtcagtTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTg                                                                                                                                                                                                                                               <  2:84359/196‑1 (MQ=255)

                                                                                                            ttATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTCATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGAGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCCACGTCGTTTTTGACCGCACAATCCTGGt                                                                                                                                                           >  4:59782/1‑274 (MQ=255)

                                                                                                                  ccAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCGAACGTCGATTGTGACAGCACACTGCGGGTGCAAtt                                                                                                                                                     >  2:440474/1‑274 (MQ=255)

                                                                                                                               gCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGTCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAgg                                                                                                                                                                                                                                                                      <  5:200843/149‑1 (MQ=255)

                                                                                                                                ccTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAggg                                                                                                                                                                                                                                                                     <  5:322627/149‑1 (MQ=255)

                                                                                                                                ccTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAggg                                                                                                                                                                                                                                                                     <  8:438777/149‑1 (MQ=255)

                                                                                                                                  ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                                                                                                                                                   >  6:406021/1‑149 (MQ=255)

                                                                                                                                     gCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTg                                                                                                                                                                                                                                                                <  8:391944/149‑1 (MQ=255)

                                                                                                                                     gCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTg                                                                                                                                                                                                                                                                >  5:373946/1‑149 (MQ=255)

                                                                                                                                          cTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTg                                                                                                                                                                                                                                                  >  1:7456/1‑158 (MQ=255)

                                                                                                                                          cTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAACACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACCATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTggtt                                                                                                                            >  1:120006/1‑273 (MQ=255)

                                                                                                                                            aTCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTcc                                                                                                                                                                                                                                                         <  8:259914/149‑1 (MQ=255)

                                                                                                                                             tCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                                                                                                                                                                                                                                                        >  7:45088/1‑149 (MQ=255)

                                                                                                                                             tCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                                                                                                                                                                                                                                                        >  6:352047/1‑149 (MQ=255)

                                                                                                                                                           gggAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGt                                                                                                                                                                                                                                         <  7:438495/149‑1 (MQ=255)

                                                                                                                                                             gAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAt                                                                                                                                                                                                                                                                  <  8:143193/123‑1 (MQ=255)

                                                                                                                                                                ttCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCAAAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGGGGAAATTCCCa                                                                                                      >  2:501331/1‑275 (MQ=255)

                                                                                                                                                                       cgcgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCa                                                                                                                                                                                                                             <  8:257668/149‑1 (MQ=255)

                                                                                                                                                                       cgcgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCa                                                                                                                                                                                                                             <  7:262016/149‑1 (MQ=255)

                                                                                                                                                                        gcgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAg                                                                                                                                                                                                                            >  8:218059/1‑149 (MQ=255)

                                                                                                                                                                        gcgTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGACAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTcgc                                                                                              >  2:288525/1‑275 (MQ=255)

                                                                                                                                                                            ggCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCaaa                                                                                                                                                                                                                        >  5:464033/1‑149 (MQ=255)

                                                                                                                                                                             gCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCaaaa                                                                                                                                                                                                                       <  8:158223/149‑1 (MQ=255)

                                                                                                                                                                               ccTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACcac                                                                                                                                                                                                                                 >  5:175509/1‑137 (MQ=255)

                                                                                                                                                                                cTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAggg                                                                                                                                                                                                                    >  8:311985/1‑149 (MQ=255)

                                                                                                                                                                                        ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACgg                                                                                                                                                                                                            <  6:464033/149‑1 (MQ=255)

                                                                                                                                                                                        ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCCCAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCCCCata                                                                              >  2:232711/1‑275 (MQ=255)

                                                                                                                                                                                        ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCCCAata                                                                              >  2:473398/1‑275 (MQ=255)

                                                                                                                                                                                          tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACggtg                                                                                                                                                                                                          >  5:384321/1‑149 (MQ=255)

                                                                                                                                                                                          tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACggtg                                                                                                                                                                                                          <  6:384321/149‑1 (MQ=255)

                                                                                                                                                                                           gAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACggtgg                                                                                                                                                                                                         >  6:230683/1‑149 (MQ=255)

                                                                                                                                                                                              agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTcc                                                                                                                                                                                                      <  5:344808/149‑1 (MQ=255)

                                                                                                                                                                                               gaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCt                                                                                                                                                                                                     >  5:7644/1‑149 (MQ=255)

                                                                                                                                                                                                aagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCtt                                                                                                                                                                                                    <  6:358755/149‑1 (MQ=255)

                                                                                                                                                                                                 agGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                                                                                                                                                   <  5:17103/149‑1 (MQ=255)

                                                                                                                                                                                                 agGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                                                                                                                                                   >  8:206383/1‑149 (MQ=255)

                                                                                                                                                                                                    tCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACt                                                                                                                                                                                                >  7:101891/1‑149 (MQ=255)

                                                                                                                                                                                                     cgggcgcAAGCCGTTGTCAGTGCCGGTAATACCGGGGGGCTGATGGGGCGGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTgaagaag                                                                  <  4:232707/271‑1 (MQ=255)

                                                                                                                                                                                                     cGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgc                                                                                                                                                                                                                                                      >  6:199547/1‑95 (MQ=255)

                                                                                                                                                                                                      gAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCCGGCAAAATTATTACTCAAGCTCCTGGAGGGGTCTGTGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGACCTTGACCTAGGGGTCAACGTCGAtt                                                                                                                                                                              >  2:390879/1‑165 (MQ=255)

                                                                                                                                                                                                        gcgcAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAgg                                                                                                                                                                                            <  8:101891/149‑1 (MQ=255)

                                                                                                                                                                                                            aaGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGcc                                                                                                                                                                                        >  8:120560/1‑149 (MQ=255)

                                                                                                                                                                                                                cTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGa                                                                                                                                                                                                                                                               <  7:367722/75‑1 (MQ=38)

                                                                                                                                                                                                                  gtgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                                                                                                                                  >  5:340431/1‑149 (MQ=255)

                                                                                                                                                                                                                  gtgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                                                                                                                                  <  5:230683/149‑1 (MQ=255)

                                                                                                                                                                                                                  gtgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                                                                                                                                  >  8:160692/1‑149 (MQ=255)

                                                                                                                                                                                                                   tgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCg                                                                                                                                                                                 >  6:109139/1‑149 (MQ=255)

                                                                                                                                                                                                                     tCAGTGCCGGTAATACCGGGGCGCGGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGCCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGa                                                  <  2:257546/274‑1 (MQ=255)

                                                                                                                                                                                                                      cAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAtt                                                                                                                                                                              <  8:45088/149‑1 (MQ=255)

                                                                                                                                                                                                                        gTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTg                                                                                                                                                          >  1:106816/1‑167 (MQ=255)

                                                                                                                                                                                                                             ggTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTa                                                                                                                                                                                                                                        >  4:537791/1‑84 (MQ=255)

                                                                                                                                                                                                                               tAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGca                                                                                                                                                                     >  6:83156/1‑149 (MQ=255)

                                                                                                                                                                                                                                aaTACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGcac                                                                                                                                                                    >  8:402808/1‑149 (MQ=255)

                                                                                                                                                                                                                                 aTACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTAc                                                                                                                                                                                                                                    <  2:537796/84‑1 (MQ=255)

                                                                                                                                                                                                                                       gggCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAAGATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCa                               >  2:175261/1‑275 (MQ=255)

                                                                                                                                                                                                                                        ggCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGTGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAg                              >  2:163309/1‑275 (MQ=255)

                                                                                                                                                                                                                                          ggatgtgggggCTGTGAAAGTAAGTACTCAAGGCCCTGGCGGGGATTGTGACACCGGGGC‑GGTGTACGGTAGTACAACGTCAGCAAAAAGGCAAAACGGTGGTCCTGGACGTAGGGGCCAACGTCGATTGTGAGAGCAGAATGCTGGTAAAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCGATCCGCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCg                            <  4:295544/268‑1 (MQ=255)

                                                                                                                                                                                                                                          cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTg                                                                                                                                                          <  5:25581/149‑1 (MQ=255)

                                                                                                                                                                                                                                          cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGTAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCg                            <  2:298202/275‑1 (MQ=255)

                                                                                                                                                                                                                                          cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCg                            <  2:350990/275‑1 (MQ=255)

                                                                                                                                                                                                                                          cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTAGTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCg                            <  2:440944/275‑1 (MQ=255)

                                                                                                                                                                                                                                             tGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCt                                                                                                                                       >  2:5512/1‑165 (MQ=255)

                                                                                                                                                                                                                                             tGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGGGCaa                                                                                                                                                       >  6:14560/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   ggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGcc                                                                                                                                                 <  8:467789/149‑1 (MQ=255)

                                                                                                                                                                                                                                                       ggCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGc                                                                                                                             >  2:150607/1‑165 (MQ=255)

                                                                                                                                                                                                                                                         cAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACTTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATg                                                                                                                                           <  7:122828/149‑1 (MQ=255)

                                                                                                                                                                                                                                                           aaaTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGaa                                                                                                                         <  4:150606/165‑1 (MQ=255)

                                                                                                                                                                                                                                                            aaTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGc                                                                                                                                        >  6:145992/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 ttaCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGt                                                                                                                                   <  7:160692/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                  taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTc                                          >  4:442929/1‑237 (MQ=255)

                                                                                                                                                                                                                                                                   aCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCt                                                                                                                                                              >  5:471080/1‑120 (MQ=255)

                                                                                                                                                                                                                                                                     tCATGCACCTGGAGGGGTGGTAGTGTCCGGTGCTTGTG‑ACGTTGTTCCAACATCTGCAAAAGGGAAAAACGGTGGTNCTTGACTTAGGGGCCAACGGCGATTGTGACAGCACAATGCTGGTGAAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCGGTGCTTAAAACAATCCCTTCTATCaa  <  3:120006/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                     tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAt                                                                                                                                                                               <  7:169560/101‑1 (MQ=255)

                                                                                                                                                                                                                                                                     tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg                                                                                                                               >  7:192362/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                      cAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGa                                      <  2:442934/237‑1 (MQ=255)

                                                                                                                                                                                                                                                                       aaGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAtt                                                                                                                                                                              >  1:125889/1‑100 (MQ=255)

                                                                                                                                                                                                                                                                        aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGt                                                                                            >  3:32240/1‑181 (MQ=255)

                                                                                                                                                                                                                                                                        aGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGt                                                                                            >  4:446876/1‑181 (MQ=255)

                                                                                                                                                                                                                                                                           cccTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGaa                                                                                                                         <  8:25379/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                            ccTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                                                                        <  1:32240/181‑1 (MQ=255)

                                                                                                                                                                                                                                                                            ccTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCg                                                                                        <  2:446881/181‑1 (MQ=255)

                                                                                                                                                                                                                                                                             cTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                                                                                                                                            <  6:349417/128‑1 (MQ=255)

                                                                                                                                                                                                                                                                             cTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                                                                                                                                            >  5:349417/1‑128 (MQ=255)

                                                                                                                                                                                                                                                                              tGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCGGTGCTTAAAACAATCCCTTc        >  2:378910/1‑259 (MQ=255)

                                                                                                                                                                                                                                                                               ggAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                                                                                                                                           >  4:530742/1‑63 (MQ=255)

                                                                                                                                                                                                                                                                                gAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                                                                                                                                            >  6:320340/1‑125 (MQ=255)

CGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTG‑ACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCGACAGTATTCGGGATGCCTCAGCGGTGCTTAAAACAATCCCTTCTATCAA  >  NC_000913/1147715‑1148249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`