| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,449,050 | T→A | 8.5% | intergenic (+32/+26) | feaB → / ← tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,449,050 | 0 | T | A | 8.5% | 287.0 / 11.9 | 90 | intergenic (+32/+26) | feaB/tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (44/37); new base A (4/4); total (48/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
GGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAGACCGGTGTCATGAGTAGAACGGTTCGGATATTTGCCTTCCGGG > NC_000913/1448787‑1449313 | ggCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTAGTAATCTGGTTCGCTCATAAGTAAAAAACGGAACCTGGGGCCGtttt > 2:447615/1‑275 (MQ=255) gAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTTGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACa < 2:347991/275‑1 (MQ=255) ggCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACt < 2:461750/275‑1 (MQ=255) aaCTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGATTTTTTGTCTGAAACAATCACTTAt > 2:70669/1‑273 (MQ=255) aaCTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACAGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAACACGGCACCTGGTGCCGATTCTGTGTCTGACACAATCACTTAtc > 2:475398/1‑274 (MQ=255) gtgtCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAg < 2:128749/275‑1 (MQ=255) gggtCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAg < 2:209449/273‑1 (MQ=255) gtCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAgc < 2:70707/274‑1 (MQ=255) tCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGCAACCATCACTTATGTTTTTTAAgagc > 1:322/1‑275 (MQ=255) taGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTCTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATCTTGGCCCCGCCTGGCTGGACGGTTGGTGTGCAACTAAGTCGGTGGGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATGTTTCGTCAGCGCCCCTAGCGTTGGCGTTTCGCCAAAGAAGTTCCAt > 2:179538/1‑275 (MQ=255) actgcctACAGGCAGGGACGGGTATGGG‑AAA‑CAGCCATACCTGAATGGCCGCCAACGTACCCTTTGGTCGGATGAAGCAGTCAGGACCGGGCCGTGATTTTGGCCCCGGCTTGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCCCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGttt < 4:447610/269‑1 (MQ=255) aTCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGCTTGGTGGGATGAAGCAGTCAGGCACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGTACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTCCTTCAGCGCCCCTAGTGTTGGCGTTTCGTCAAAGAAGTTCCATGGtt > 2:374609/1‑274 (MQ=255) tACGGGCAGGGA‑‑GGTGTGGGTAAACCAGCCAACCCTTAATTGACGGCAATGTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGAGTTTGGCCCCGACTGGCTGGACGGTAGATGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagca < 4:374604/274‑1 (MQ=255) tACAGGCAGGGAC‑GGTGTTGGTAAA‑CAGCAATACCTTAATTGACGATAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagcag < 1:114028/275‑1 (MQ=255) aggGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTACTCTGGTTCGCTCATAAGTAAAAAACGGACCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCTCCCCTAGCGTTGGCTATTCGTCAAAGAAGTTCCATGGTTTCCGCAGCGtcttt > 4:75513/1‑271 (MQ=255) aC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagc < 2:75514/262‑1 (MQ=255) aa‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCa > 2:108376/1‑275 (MQ=255) cAGCCATACCTTAATTGACGCTAACTTACCGATTGGCGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGATGGTGTGAACCTAAGTCGGCGTGTGTACGGTATTAATGTGTCTCGCTCACAAGTAAAAAACGGCGCCTGGTGCCGTTTTGTTGTCTGACACAATCACTTATCTTTCTTCAGCGCACTTGCCGTTGGCTTTGCGTAAAAGAAGTTCCATGGTTNNNCCAGGGTGACTAGCCCTtcggtcagacac > 3:156256/1‑269 (MQ=255) aTTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGt > 6:350883/1‑149 (MQ=255) ttGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTg > 8:476018/1‑149 (MQ=255) aCGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTg > 4:477803/1‑199 (MQ=255) ctcTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTTAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGgcg < 1:156256/273‑1 (MQ=255) cGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTca > 2:11473/1‑228 (MQ=255) gCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACAGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCACAGAAGTTCCATGGTTTCAGCAGAGTATGTAACCATTCGGTCGGCCTA‑ATCGGCCACCCTTCGgcga > 2:85917/1‑274 (MQ=255) cTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGttt < 5:240017/149‑1 (MQ=255) tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGt < 2:477808/199‑1 (MQ=255) tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttt < 6:162895/149‑1 (MQ=255) tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttt < 7:451223/149‑1 (MQ=255) tggtggGATGAAGCAGTCAGGAACGGGCCGTGTTTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCCCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGNAGTTCCATGGTTCCATCAGAGTATGTAACCATGCGGTCGGTCTACGTCGGCCCCT‑TTCGTCGCGGGTCACCtgttg > 3:116263/1‑272 (MQ=255) tggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAAt < 7:393866/149‑1 (MQ=255) gATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCAc > 6:217912/1‑149 (MQ=255) aTGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACt > 8:193452/1‑149 (MQ=255) gTCAGGAACGGGCCGTGATTTTGGCGCCGACTGGCTGGACGGTTAGTGTGAACCTAAGTCGGTGTGTGTACGGTATTAAGCTGGTTCGATCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtctttc < 8:6162/149‑1 (MQ=255) tCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtctttct > 8:315616/1‑149 (MQ=255) aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTttcttc > 8:382029/1‑149 (MQ=255) ggAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCa < 7:382029/149‑1 (MQ=255) gAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCa < 6:29317/148‑1 (MQ=255) gAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCa > 5:29317/1‑148 (MQ=255) aaCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAgc > 8:126054/1‑149 (MQ=255) gggCGGTGATTTTTGCCCCGACTGGCTGGACGGTGGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACaa < 1:116263/273‑1 (MQ=255) gCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTTTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaag > 2:497146/1‑174 (MQ=255) ccGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCt < 7:476018/149‑1 (MQ=255) tGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc < 4:497141/174‑1 (MQ=255) gACTGGTTGTACTGGTGGTGTTAAACTAAGTCGGTGTGTGTACGGTATTAATTGGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACTATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑g < 2:479094/275‑1 (MQ=255) tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa < 7:315616/149‑1 (MQ=255) tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa > 7:353990/1‑149 (MQ=255) ttGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCAt < 5:354870/149‑1 (MQ=255) gtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTCGCGTCGGCGTTTCGTCAAAGAAGTTCCATGGTTTca > 4:84601/1‑151 (MQ=255) gtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTGTCCTTCATGTa > 4:127061/1‑275 (MQ=255) aaCTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGGAGTTCCATGGTTTcagcag < 2:84602/151‑1 (MQ=255) tAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCaga < 7:193452/149‑1 (MQ=255) aGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTGGCGTTGGCGTTTCGTCACAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ACCGGCCACTCTTCGGCGCGGACCACTTGTGTGGTCCCGGTGGTCAGCCAGACTACGGCGTCGGTGTTGTCCGGC‑GCTCCGTTACTCTCACATTATTGTCCAAg > 4:347954/1‑275 (MQ=255) aGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAg > 2:60639/1‑275 (MQ=255) aGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAg > 2:83021/1‑275 (MQ=255) ggTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCATAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAGCCATTCGGTCGGCCTA‑ATCGGCCACTCTTCGGCGTGGGCCACATGTGTGGTGCCGGTGGTCATCCAGCAAACGGGTTCGG‑GTTTTGAACCGGNNNCGTTCTCCTGACTGTATTGTCCCAGAcc > 3:156110/1‑274 (MQ=255) gtgtttgtTCGGTATTAATCTGGTTTGCTCATAAGTAAAAAACGGCACCGGGTGCCGTTTTTTTGTCTGAAAAAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAGACCg < 4:83020/274‑1 (MQ=255) gtgtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAGACCg < 2:127062/274‑1 (MQ=255) gtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAcc > 6:17301/1‑149 (MQ=255) tgtgtACGGTATTAATCTGGTTCGCTCATAAGGAAAAAACGGCCCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTAt < 1:156110/259‑1 (MQ=255) tgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAt < 8:196454/149‑1 (MQ=255) gTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCAAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTCTCCTTACTGTATTGTCCAATACCGGTGTCCTGCGtt > 1:17797/1‑274 (MQ=255) tAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACNCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACACCgncgnc > 4:548021/1‑213 (MQ=255) aTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGAGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGTCATA‑CTCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGTGCCTCCCGACAACGGCGTCGGTATTTTCCAGC‑GACTCGTTCTCCATACTGTATTGTCCCAGACCGGTGTCCTGAGTAgccct > 4:218292/1‑270 (MQ=255) cTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGgtg < 2:548026/213‑1 (MQ=255) ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAt < 8:149908/133‑1 (MQ=255) ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAt > 7:149908/1‑133 (MQ=255) gTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTc > 2:285853/1‑114 (MQ=255) gTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGCTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGCTTCAGCAGAGTATGTACCCATtcggtc > 2:78777/1‑138 (MQ=255) ttCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGc < 5:173934/149‑1 (MQ=255) ttCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGc > 6:173934/1‑149 (MQ=255) ttCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGc < 7:80441/149‑1 (MQ=255) gCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagca < 4:285849/114‑1 (MQ=255) cTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtctttctt > 2:429797/1‑63 (MQ=255) tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGtt < 5:22764/78‑1 (MQ=255) tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGtt > 6:22764/1‑78 (MQ=255) tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc > 6:44868/1‑100 (MQ=255) tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc < 5:44868/100‑1 (MQ=255) aTAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCtt < 5:17301/149‑1 (MQ=255) tAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAgc < 4:429792/63‑1 (MQ=255) aaaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCa > 8:330680/1‑92 (MQ=255) aaaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCa < 7:330680/92‑1 (MQ=255) aaaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGtgcg > 7:197845/1‑149 (MQ=255) aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCgg > 7:53937/1‑149 (MQ=255) aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTAGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTTCAGC‑GACTCGTTATACTTACTGTATTGTCCAAGACCGGTGTCATGAGTAGAACGGTTCGGATCTTTGCCTTCCggt > 2:510614/1‑274 (MQ=255) aaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCgg > 8:58020/1‑149 (MQ=255) aaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCa < 8:222781/79‑1 (MQ=255) aaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCa > 7:222781/1‑79 (MQ=255) aaaCGGCACCACGCGGCGTTTTATTGTCTGCAACAATCACTTATCTTTCTGCAGCGCCCCTAGCGTTGGCGTGGCGTCAAACAAGCTCCTTGTTTTCAGCAGCGTATGTAGCCATTCGGTCCGCAAA‑GTCGGCAACTCTTCGCCGCggg > 8:381567/1‑149 (MQ=255) aaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGc > 6:437958/1‑149 (MQ=255) cACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACAt < 5:350883/149‑1 (MQ=255) tGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACAtgtgt > 5:343528/1‑149 (MQ=255) | GGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTCTCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGAC‑GGTGTGGGTAAA‑CAGCCATACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATA‑ATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCCAGC‑GACTCGTTATCCTTACTGTATTGTCCAAGACCGGTGTCATGAGTAGAACGGTTCGGATATTTGCCTTCCGGG > NC_000913/1448787‑1449313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |