| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,578,178 | Δ1 bp | 9.1% | coding (200/2280 nt) | maeB ← | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,578,178 | 0 | A | . | 9.1% | 146.4 / 8.9 | 44 | coding (200/2280 nt) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
| Reads supporting (aligned to +/- strand): ref base A (23/17); new base . (2/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCC > NC_000913/2578034‑2578323 | tCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAg < 1:103857/149‑1 (MQ=255) tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc > 3:280692/1‑149 (MQ=255) tCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAAc < 3:279634/149‑1 (MQ=255) cccGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACg > 3:267527/1‑149 (MQ=255) ggCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGt > 4:55088/1‑149 (MQ=255) aaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc < 3:243744/149‑1 (MQ=255) aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa > 4:153402/1‑121 (MQ=255) aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa < 3:153402/121‑1 (MQ=255) aCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa > 4:59285/1‑149 (MQ=255) aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc > 2:231656/1‑149 (MQ=255) aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc > 2:426086/1‑149 (MQ=255) gCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAg < 4:280692/149‑1 (MQ=255) ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTTGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt > 4:106892/1‑149 (MQ=255) ccTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt < 3:89772/149‑1 (MQ=255) gCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCgg < 2:170439/149‑1 (MQ=255) ttCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCaa > 3:153500/1‑149 (MQ=255) aCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTgg > 2:462567/1‑149 (MQ=255) aGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCCTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcgc > 2:391686/1‑149 (MQ=255) ccAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATc > 4:373980/1‑149 (MQ=255) cAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcg > 1:472374/1‑149 (MQ=255) ttGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCGATCTGGCGCtg < 2:453435/149‑1 (MQ=255) cTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt > 3:474248/1‑149 (MQ=255) tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt < 4:310217/92‑1 (MQ=255) tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt > 3:310217/1‑92 (MQ=255) ccccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCNCGCTGTGTTGCCa < 1:426086/149‑1 (MQ=255) ccAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCaga < 1:462567/149‑1 (MQ=255) cAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCagag > 1:277628/1‑149 (MQ=255) ccgTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCttggtt < 1:231656/149‑1 (MQ=255) gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa < 4:246306/79‑1 (MQ=255) gTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCaa > 3:246306/1‑79 (MQ=255) aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc < 1:127451/87‑1 (MQ=255) aCCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc > 2:127451/1‑87 (MQ=255) ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc < 4:267527/149‑1 (MQ=255) ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc > 2:320232/1‑149 (MQ=255) ttAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAAc > 2:26083/1‑149 (MQ=255) tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt > 3:320824‑M1/10‑126 (MQ=255) tacaaatatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt < 4:320824‑M1/117‑1 (MQ=255) gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa > 4:238536/1‑41 (MQ=255) gagaTCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTa < 3:238536/41‑1 (MQ=255) atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg < 1:351321‑M1/93‑1 (MQ=255) atatACCGCC‑CGAGGTAACCTGGTGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAg > 2:351321‑M1/5‑97 (MQ=255) cACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAttt > 2:472352/1‑149 (MQ=255) ACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGAtttt < 3:454969‑M1/149‑1 (MQ=255) GCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTccc > 4:153500‑M1/1‑149 (MQ=255) | TCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCG‑GGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAGAGGCTTGGTTGGAGAAACCTGGATTTTCCC > NC_000913/2578034‑2578323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |