Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,470,501:1	+T	8.2%	intergenic (+180/+26)	mgtA → / ← ridA	magnesium transporter/enamine/imine deaminase, reaction intermediate detoxification

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,470,501	1	.	T	8.2%	201.1 / 13.4	49	intergenic (+180/+26)	mgtA/ridA	magnesium transporter/enamine/imine deaminase, reaction intermediate detoxification
Reads supporting (aligned to +/- strand):  ref base . (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

GATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTG  >  NC_000913/4470353‑4470647                                                                                                                                                      |                                                                                                                                                   gATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGc                                                                                                                                                     >  1:65719/1‑149 (MQ=255)                   ttttGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTcc                                                                                                                                  >  2:418406/1‑149 (MQ=255)                   ttttGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTcc                                                                                                                                  <  1:418406/149‑1 (MQ=255)                        ttCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCaa                                                                                                                             <  2:54363/149‑1 (MQ=255)                          cATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGa                                                                                                                           <  2:396621/149‑1 (MQ=255)                           aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAttaa                                                                                                                                   >  2:439962/1‑138 (MQ=37)                           aTGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAttaa                                                                                                                                   <  1:439962/140‑3 (MQ=37)                               cGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGc                                                                                                                      <  4:414345/149‑1 (MQ=255)                                  aTGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGACAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGcgac                                                                                                                   <  3:244542/149‑1 (MQ=255)                                     cggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGaa                                                                                                                <  4:317224/149‑1 (MQ=255)                                     cggcgTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGaa                                                                                                                <  3:248059/149‑1 (MQ=255)                                         gTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGc                                                                                                            >  2:94847/1‑149 (MQ=255)                                                 cTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAtta                                                                                                                                    <  1:301702/117‑2 (MQ=25)                                                 cTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGCTATCAT‑GCCCGGAtta                                                                                                                                    >  2:301702/1‑116 (MQ=25)                                                            aacaaATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATc                                                                                         >  3:462800/1‑149 (MQ=255)                                                            aacaaATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATc                                                                                         >  4:441425/1‑149 (MQ=255)                                                                  ttCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGcc                                                                                                                                           >  1:459189/1‑93 (MQ=255)                                                                  ttCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGcc                                                                                                                                           <  2:459189/93‑1 (MQ=255)                                                                     aaTAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCt                                                                                >  3:426188/1‑149 (MQ=255)                                                                       tAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCa                                                                                     <  2:300259/142‑1 (MQ=255)                                                                       tAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCa                                                                                     >  1:300259/1‑142 (MQ=255)                                                                        aaaTTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTc                                                                             <  4:426188/149‑1 (MQ=255)                                                                          aTTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCgg                                                                           <  2:192525/149‑1 (MQ=255)                                                                                      aCGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAAc                                                               <  2:65719/149‑1 (MQ=255)                                                                                        gTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACtt                                                             <  1:94847/149‑1 (MQ=255)                                                                                            gCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAAc                                                         >  2:391407/1‑149 (MQ=255)                                                                                            gCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAAc                                                         >  2:48386/1‑149 (MQ=255)                                                                                             ccTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACg                                                        >  2:181605/1‑149 (MQ=255)                                                                                             ccTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACg                                                        >  1:446703/1‑149 (MQ=255)                                                                                                     gCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACg                                                <  3:410110/149‑1 (MQ=255)                                                                                                          gcgcATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCg                                           <  1:372395/149‑1 (MQ=255)                                                                                                               tCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAg                                      >  3:312388/1‑149 (MQ=255)                                                                                                                      aTTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGt                               <  4:462800/149‑1 (MQ=255)                                                                                                                                 gTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTg                    <  4:312388/149‑1 (MQ=255)                                                                                                                                      caGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTgaagaa               >  3:56680/1‑149 (MQ=255)                                                                                                                                      caGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTgaagaa               <  4:104434/149‑1 (MQ=255)                                                                                                                                       aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGc                                                                                                      <  3:350606/61‑1 (MQ=255)                                                                                                                                       aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGc                                                                                                      >  4:350606/1‑61 (MQ=255)                                                                                                                                       aGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGaagaag              >  3:218430/1‑149 (MQ=255)                                                                                                                                        gACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAgg             <  1:181605/149‑1 (MQ=255)                                                                                                                                         aCTTAATCCGAGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCTAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGc            >  4:309389/1‑149 (MQ=255)                                                                                                                                          cTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGt                     >  1:246953/1‑139 (MQ=255)                                                                                                                                          cTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGt                     <  2:246953/139‑1 (MQ=255)                                                                                                                                           ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          >  3:436067/1‑149 (MQ=255)                                                                                                                                           ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          <  4:218430/149‑1 (MQ=255)                                                                                                                                           ttAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCtt          >  1:277410/1‑149 (MQ=255)                                                                                                                                                  gggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGt                                               >  4:261572/1‑105 (MQ=255)                                                                                                                                                  gggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGt                                               <  3:261572/105‑1 (MQ=255)                                                                                                                                                   ggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTg  <  1:48386/149‑1 (MQ=255)                                                                                                                                                   ggC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTg  <  3:309389/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GATTGTAGACAAAGTGAGTTTTGTTCATGCCGGATGCGGCGTAAATGCCTTATCCGGCCAACAAATTCAATAAATTGCAGGAATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTTTGCGTTTGTCATCAGACTTAATCCGGGC‑ATGATAGCCCGGATTTCCATCAAGATTAGCGACGAACAGCGATCGCTTCGATCTCAATCTTCACGTCTTTCGGCAGACGGGCAACTTCAACGCAAGAACGTGCCGGGAAGGTGGCGTTGTGTTCGGTGAAGAAGGCTTCGTAAGTG  >  NC_000913/4470353‑4470647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑